Candidatus Frankia datiscae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Frankiales; Frankiaceae; Frankia

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4063 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F8B5L1|F8B5L1_9ACTN Uncharacterized protein OS=Candidatus Frankia datiscae OX=2716812 GN=FsymDg_1731 PE=4 SV=1
MM1 pKa = 7.65GGFQRR6 pKa = 11.84ACALSDD12 pKa = 3.63LSEE15 pKa = 5.36DD16 pKa = 3.09IPLGVEE22 pKa = 3.76VDD24 pKa = 4.0GEE26 pKa = 4.49PVCLALAGGQVYY38 pKa = 9.9AVRR41 pKa = 11.84DD42 pKa = 3.87EE43 pKa = 4.73CSHH46 pKa = 7.31SDD48 pKa = 3.26VMLSQGEE55 pKa = 4.14IEE57 pKa = 4.33DD58 pKa = 4.17AKK60 pKa = 10.7IEE62 pKa = 4.23CWLHH66 pKa = 6.41GSQFDD71 pKa = 4.19LSTGEE76 pKa = 4.15PLGLPATEE84 pKa = 4.42PVPVYY89 pKa = 9.72PVKK92 pKa = 10.38IDD94 pKa = 3.69GDD96 pKa = 4.11DD97 pKa = 3.48VLVDD101 pKa = 3.58VGEE104 pKa = 4.35SS105 pKa = 3.14

Molecular weight: 11.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F8B0B6|F8B0B6_9ACTN CopG-like domain-containing protein DNA-binding protein OS=Candidatus Frankia datiscae OX=2716812 GN=FsymDg_1251 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AVLSARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.66GRR40 pKa = 11.84SEE42 pKa = 3.94LSAA45 pKa = 4.73

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4063

0

4063

1333214

30

6077

328.1

34.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.781 ± 0.066

0.814 ± 0.011

6.109 ± 0.027

4.894 ± 0.034

2.652 ± 0.02

9.51 ± 0.043

2.247 ± 0.017

3.542 ± 0.026

1.481 ± 0.024

10.034 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.535 ± 0.013

1.721 ± 0.017

6.571 ± 0.036

2.665 ± 0.022

8.836 ± 0.035

5.341 ± 0.029

6.22 ± 0.028

8.819 ± 0.037

1.368 ± 0.013

1.862 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski