Caenorhabditis briggsae

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 21755 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G2J6X4|G2J6X4_CAEBR Protein CBG17140 OS=Caenorhabditis briggsae OX=6238 GN=CBG17140 PE=4 SV=1
MM1 pKa = 7.54PRR3 pKa = 11.84YY4 pKa = 9.99SLLLLIIIPIANGYY18 pKa = 9.46IFDD21 pKa = 4.27PSRR24 pKa = 11.84TRR26 pKa = 11.84KK27 pKa = 9.8VLIVPKK33 pKa = 10.46NKK35 pKa = 10.4NGWHH39 pKa = 7.16DD40 pKa = 3.75SATFAPIDD48 pKa = 4.5PIQIGTQDD56 pKa = 3.41WEE58 pKa = 4.44DD59 pKa = 3.42LTEE62 pKa = 3.92VEE64 pKa = 5.03RR65 pKa = 11.84YY66 pKa = 9.41YY67 pKa = 11.37LEE69 pKa = 4.14TKK71 pKa = 9.02GTLRR75 pKa = 11.84FSGSSAYY82 pKa = 10.27DD83 pKa = 3.24YY84 pKa = 11.67GNQEE88 pKa = 4.46LKK90 pKa = 10.79DD91 pKa = 4.42DD92 pKa = 4.54YY93 pKa = 11.55DD94 pKa = 4.06VSDD97 pKa = 5.15YY98 pKa = 11.02IDD100 pKa = 4.54EE101 pKa = 4.32EE102 pKa = 4.41DD103 pKa = 5.88KK104 pKa = 11.51EE105 pKa = 5.23DD106 pKa = 4.86DD107 pKa = 4.06DD108 pKa = 4.91EE109 pKa = 6.35KK110 pKa = 11.55GSGDD114 pKa = 3.54YY115 pKa = 11.47GDD117 pKa = 3.82VSKK120 pKa = 11.15EE121 pKa = 4.18DD122 pKa = 3.6EE123 pKa = 4.24DD124 pKa = 4.55SEE126 pKa = 5.0YY127 pKa = 11.01EE128 pKa = 4.36GYY130 pKa = 10.9LEE132 pKa = 6.29DD133 pKa = 5.82EE134 pKa = 4.69FDD136 pKa = 5.63DD137 pKa = 4.07YY138 pKa = 12.06DD139 pKa = 3.86NQYY142 pKa = 11.24EE143 pKa = 4.19YY144 pKa = 11.48DD145 pKa = 3.59NYY147 pKa = 11.67

Molecular weight: 17.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8Y3V3|A8Y3V3_CAEBR Protein CBR-SNB-2 OS=Caenorhabditis briggsae OX=6238 GN=snb-2 PE=4 SV=1
LL1 pKa = 6.68ITPSPAPIPALPVPIPHH18 pKa = 7.37RR19 pKa = 11.84PTPIPPPSALIQSPPAPIPPSPASIPPSPAPIPHH53 pKa = 7.35PLAPIPLTPAAIPLPSAPIPPPPAPIQSSPALIPPPPAPIPPPPALIPPPPALIPPPPAPIPPSPAPIPPSPAPIPPPPPAPIPPPPAPIPPPPAPIPPPPAPIPPPPAPIPPPPAPIPPPSSPIPPPPAPIPPPPAPIPPPILRR198 pKa = 11.84FHH200 pKa = 6.61LHH202 pKa = 4.54QLRR205 pKa = 11.84FHH207 pKa = 6.51LHH209 pKa = 4.2QLRR212 pKa = 11.84FHH214 pKa = 6.51LHH216 pKa = 4.2QLRR219 pKa = 11.84FHH221 pKa = 6.51LHH223 pKa = 4.2QLRR226 pKa = 11.84FHH228 pKa = 6.51LHH230 pKa = 4.2QLRR233 pKa = 11.84FHH235 pKa = 6.51LHH237 pKa = 4.2QLRR240 pKa = 11.84FHH242 pKa = 6.72LHH244 pKa = 5.02QLHH247 pKa = 6.23LHH249 pKa = 4.91QLRR252 pKa = 11.84FHH254 pKa = 6.51LHH256 pKa = 4.2QLRR259 pKa = 11.84FHH261 pKa = 6.51LHH263 pKa = 4.2QLRR266 pKa = 11.84FHH268 pKa = 6.42LHH270 pKa = 5.85HH271 pKa = 6.88LRR273 pKa = 11.84FHH275 pKa = 6.37LHH277 pKa = 4.47QLRR280 pKa = 11.84FHH282 pKa = 6.51LHH284 pKa = 4.2QLRR287 pKa = 11.84FHH289 pKa = 6.51LHH291 pKa = 4.2QLRR294 pKa = 11.84FHH296 pKa = 6.51LHH298 pKa = 4.2QLRR301 pKa = 11.84FHH303 pKa = 6.74LHH305 pKa = 4.42QLL307 pKa = 3.48

Molecular weight: 33.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21727

28

21755

8683488

8

13580

399.1

45.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.222 ± 0.02

1.963 ± 0.015

5.288 ± 0.017

6.851 ± 0.026

4.681 ± 0.016

5.342 ± 0.027

2.276 ± 0.009

5.971 ± 0.016

6.432 ± 0.02

8.476 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.639 ± 0.009

4.827 ± 0.012

5.08 ± 0.025

4.16 ± 0.019

5.368 ± 0.018

8.066 ± 0.025

5.788 ± 0.024

6.134 ± 0.015

1.121 ± 0.006

3.118 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski