candidate division MSBL1 archaeon SCGC-AAA382A03

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Euryarchaeota incertae sedis; candidate division MSBL1

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 427 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A133VG05|A0A133VG05_9EURY F420_ligase domain-containing protein OS=candidate division MSBL1 archaeon SCGC-AAA382A03 OX=1698278 GN=AKJ49_00930 PE=4 SV=1
MM1 pKa = 7.42GGTYY5 pKa = 10.64GSLDD9 pKa = 3.7STSGSSPGFSATSEE23 pKa = 4.23TGDD26 pKa = 3.6HH27 pKa = 6.69SSHH30 pKa = 6.97DD31 pKa = 3.72VTVEE35 pKa = 3.78LTVTDD40 pKa = 4.64DD41 pKa = 5.8DD42 pKa = 5.0GATDD46 pKa = 3.62TDD48 pKa = 4.11TATVTVNSQNDD59 pKa = 3.8PPTTSSDD66 pKa = 4.33SITTCEE72 pKa = 4.04NTSGSSDD79 pKa = 3.49VLFNDD84 pKa = 4.25SDD86 pKa = 4.41PEE88 pKa = 4.3GDD90 pKa = 3.43PLVYY94 pKa = 10.77SNVSISTSPSHH105 pKa = 6.57GSASVDD111 pKa = 3.25EE112 pKa = 4.45TAGEE116 pKa = 4.19IVYY119 pKa = 10.69SPNPNYY125 pKa = 10.54NGSDD129 pKa = 3.09SFEE132 pKa = 4.07YY133 pKa = 10.46QISDD137 pKa = 3.56GNGGISTEE145 pKa = 4.41TVSVTVNNVSDD156 pKa = 3.84SPTALFTHH164 pKa = 6.5SSSDD168 pKa = 3.2KK169 pKa = 11.07VLDD172 pKa = 3.53VDD174 pKa = 4.94ASNSSDD180 pKa = 2.94IDD182 pKa = 3.81GSISSYY188 pKa = 6.88EE189 pKa = 4.21WKK191 pKa = 9.82WSSGDD196 pKa = 3.62SFSSGTEE203 pKa = 4.13TDD205 pKa = 2.85SHH207 pKa = 6.55TYY209 pKa = 10.35SSGGEE214 pKa = 3.92YY215 pKa = 9.73TVEE218 pKa = 4.7LRR220 pKa = 11.84VTDD223 pKa = 4.67DD224 pKa = 5.01DD225 pKa = 4.81GATDD229 pKa = 4.1TYY231 pKa = 10.98SQTVTINKK239 pKa = 10.03DD240 pKa = 2.79PDD242 pKa = 3.99APSSPSPSDD251 pKa = 3.41GVKK254 pKa = 10.51LSEE257 pKa = 4.25TSSVTLEE264 pKa = 4.22VTVTDD269 pKa = 4.27PDD271 pKa = 4.27GDD273 pKa = 4.17SMDD276 pKa = 3.42VAFYY280 pKa = 10.98DD281 pKa = 4.49SGGTQIGTTQTGVSDD296 pKa = 3.95GGTTSVSYY304 pKa = 10.65SVSAGNSYY312 pKa = 11.1DD313 pKa = 3.2WYY315 pKa = 11.07AVATDD320 pKa = 4.66DD321 pKa = 5.13EE322 pKa = 5.02GASTQSSTWSFTVKK336 pKa = 10.49PPVDD340 pKa = 3.4EE341 pKa = 4.68SKK343 pKa = 11.32EE344 pKa = 3.94EE345 pKa = 4.1DD346 pKa = 4.88EE347 pKa = 4.61ITQTYY352 pKa = 10.13NLLPILIVLSLSYY365 pKa = 10.76SITLFLSKK373 pKa = 10.22RR374 pKa = 11.84KK375 pKa = 10.04SISTLTHH382 pKa = 4.93KK383 pKa = 10.69RR384 pKa = 11.84IWNVFLLITFLASGILGILLVIRR407 pKa = 11.84INFGFVIPLPFNILFWHH424 pKa = 6.03VEE426 pKa = 3.44AGIAMFVICIFHH438 pKa = 6.57IIEE441 pKa = 4.34RR442 pKa = 11.84FRR444 pKa = 11.84ALLWFLL450 pKa = 4.05

Molecular weight: 47.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A133VG00|A0A133VG00_9EURY PKD domain-containing protein OS=candidate division MSBL1 archaeon SCGC-AAA382A03 OX=1698278 GN=AKJ49_00995 PE=4 SV=1
MM1 pKa = 7.55TNPEE5 pKa = 4.07VEE7 pKa = 4.33PTNNRR12 pKa = 11.84AEE14 pKa = 3.97RR15 pKa = 11.84STRR18 pKa = 11.84KK19 pKa = 9.48IVTLRR24 pKa = 11.84KK25 pKa = 9.59IIGTVRR31 pKa = 11.84SEE33 pKa = 3.71RR34 pKa = 11.84GRR36 pKa = 11.84YY37 pKa = 8.56ILEE40 pKa = 4.54TIMTTIEE47 pKa = 3.53TWKK50 pKa = 10.73ARR52 pKa = 11.84GQNPHH57 pKa = 7.17NEE59 pKa = 3.98MQKK62 pKa = 9.91ILRR65 pKa = 11.84NSS67 pKa = 3.16

Molecular weight: 7.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

427

0

427

103200

55

1225

241.7

27.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.797 ± 0.146

0.953 ± 0.048

5.909 ± 0.106

10.261 ± 0.186

3.933 ± 0.099

6.738 ± 0.131

1.668 ± 0.047

7.548 ± 0.122

8.874 ± 0.174

8.82 ± 0.123

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.198 ± 0.055

4.342 ± 0.073

3.944 ± 0.077

2.492 ± 0.055

5.074 ± 0.118

6.283 ± 0.105

4.828 ± 0.081

6.204 ± 0.096

1.052 ± 0.046

3.082 ± 0.087
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski