Novosphingobium barchaimii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Novosphingobium

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3126 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A147ELI9|A0A147ELI9_9SPHN Cobalamin biosynthesis protein CobW OS=Novosphingobium barchaimii OX=1420591 GN=NS277_02680 PE=3 SV=1
MM1 pKa = 7.43RR2 pKa = 11.84TTFEE6 pKa = 3.98EE7 pKa = 4.41ASVRR11 pKa = 11.84LYY13 pKa = 11.01HH14 pKa = 7.38LDD16 pKa = 3.6DD17 pKa = 5.04AGEE20 pKa = 4.49GGAATTLFYY29 pKa = 11.31GPLSEE34 pKa = 4.94ALAIAAAQDD43 pKa = 3.42EE44 pKa = 4.52ATQDD48 pKa = 3.98GLWIATDD55 pKa = 3.54NDD57 pKa = 3.6VIAYY61 pKa = 10.14LDD63 pKa = 3.96LDD65 pKa = 3.89SS66 pKa = 4.97

Molecular weight: 7.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A147EL05|A0A147EL05_9SPHN RNA polymerase sigma factor RpoH OS=Novosphingobium barchaimii OX=1420591 GN=rpoH PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.45GFRR19 pKa = 11.84ARR21 pKa = 11.84TATVGGRR28 pKa = 11.84KK29 pKa = 8.17VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3126

0

3126

995628

29

3219

318.5

34.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.983 ± 0.067

0.795 ± 0.013

5.843 ± 0.039

5.341 ± 0.041

3.522 ± 0.03

8.869 ± 0.045

1.998 ± 0.023

5.044 ± 0.032

3.059 ± 0.032

9.972 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.442 ± 0.025

2.594 ± 0.034

5.282 ± 0.033

3.359 ± 0.024

7.242 ± 0.043

5.394 ± 0.031

5.363 ± 0.033

7.267 ± 0.039

1.429 ± 0.019

2.2 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski