Amino acid dipepetide frequency for Methylobacillus sp. MM3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	12.627AlaAla: 12.627 ± 0.162	1.076AlaCys: 1.076 ± 0.042	5.512AlaAsp: 5.512 ± 0.087	7.12AlaGlu: 7.12 ± 0.128	3.731AlaPhe: 3.731 ± 0.067	8.345AlaGly: 8.345 ± 0.109	2.457AlaHis: 2.457 ± 0.06	6.027AlaIle: 6.027 ± 0.093	4.072AlaLys: 4.072 ± 0.078	12.088AlaLeu: 12.088 ± 0.15	3.341AlaMet: 3.341 ± 0.066	3.108AlaAsn: 3.108 ± 0.067	4.27AlaPro: 4.27 ± 0.087	4.469AlaGln: 4.469 ± 0.086	6.595AlaArg: 6.595 ± 0.103	5.677AlaSer: 5.677 ± 0.083	5.017AlaThr: 5.017 ± 0.083	7.366AlaVal: 7.366 ± 0.104	1.447AlaTrp: 1.447 ± 0.043	2.553AlaTyr: 2.553 ± 0.055	0.0AlaXaa: 0.0 ± 0.0
Cys	0.915CysAla: 0.915 ± 0.034	0.132CysCys: 0.132 ± 0.013	0.588CysAsp: 0.588 ± 0.028	0.518CysGlu: 0.518 ± 0.024	0.32CysPhe: 0.32 ± 0.021	0.921CysGly: 0.921 ± 0.041	0.301CysHis: 0.301 ± 0.017	0.491CysIle: 0.491 ± 0.025	0.317CysLys: 0.317 ± 0.022	0.817CysLeu: 0.817 ± 0.034	0.228CysMet: 0.228 ± 0.017	0.319CysAsn: 0.319 ± 0.017	0.506CysPro: 0.506 ± 0.026	0.263CysGln: 0.263 ± 0.015	0.602CysArg: 0.602 ± 0.026	0.5CysSer: 0.5 ± 0.027	0.46CysThr: 0.46 ± 0.022	0.651CysVal: 0.651 ± 0.032	0.107CysTrp: 0.107 ± 0.01	0.261CysTyr: 0.261 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	5.809AspAla: 5.809 ± 0.083	0.462AspCys: 0.462 ± 0.022	2.846AspAsp: 2.846 ± 0.061	3.535AspGlu: 3.535 ± 0.064	2.226AspPhe: 2.226 ± 0.056	4.194AspGly: 4.194 ± 0.081	1.171AspHis: 1.171 ± 0.037	3.468AspIle: 3.468 ± 0.067	2.473AspLys: 2.473 ± 0.055	5.15AspLeu: 5.15 ± 0.082	1.471AspMet: 1.471 ± 0.044	1.64AspAsn: 1.64 ± 0.04	2.627AspPro: 2.627 ± 0.056	1.679AspGln: 1.679 ± 0.049	3.115AspArg: 3.115 ± 0.062	2.466AspSer: 2.466 ± 0.057	2.68AspThr: 2.68 ± 0.053	3.718AspVal: 3.718 ± 0.061	0.864AspTrp: 0.864 ± 0.033	1.796AspTyr: 1.796 ± 0.045	0.0AspXaa: 0.0 ± 0.0
Glu	6.661GluAla: 6.661 ± 0.105	0.499GluCys: 0.499 ± 0.027	2.744GluAsp: 2.744 ± 0.059	3.75GluGlu: 3.75 ± 0.091	2.267GluPhe: 2.267 ± 0.06	3.854GluGly: 3.854 ± 0.072	1.544GluHis: 1.544 ± 0.038	4.112GluIle: 4.112 ± 0.068	3.291GluLys: 3.291 ± 0.075	6.376GluLeu: 6.376 ± 0.094	1.84GluMet: 1.84 ± 0.048	2.239GluAsn: 2.239 ± 0.045	2.372GluPro: 2.372 ± 0.056	2.905GluGln: 2.905 ± 0.06	4.343GluArg: 4.343 ± 0.07	3.39GluSer: 3.39 ± 0.061	3.359GluThr: 3.359 ± 0.066	4.22GluVal: 4.22 ± 0.072	0.815GluTrp: 0.815 ± 0.029	1.576GluTyr: 1.576 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	3.808PheAla: 3.808 ± 0.081	0.415PheCys: 0.415 ± 0.022	2.503PheAsp: 2.503 ± 0.056	2.247PheGlu: 2.247 ± 0.056	1.578PhePhe: 1.578 ± 0.05	3.308PheGly: 3.308 ± 0.067	0.881PheHis: 0.881 ± 0.032	2.058PheIle: 2.058 ± 0.054	1.432PheLys: 1.432 ± 0.042	3.425PheLeu: 3.425 ± 0.072	0.94PheMet: 0.94 ± 0.029	1.357PheAsn: 1.357 ± 0.041	1.605PhePro: 1.605 ± 0.054	1.081PheGln: 1.081 ± 0.035	2.064PheArg: 2.064 ± 0.045	2.489PheSer: 2.489 ± 0.043	1.952PheThr: 1.952 ± 0.054	2.719PheVal: 2.719 ± 0.058	0.536PheTrp: 0.536 ± 0.026	1.202PheTyr: 1.202 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	6.868GlyAla: 6.868 ± 0.111	0.883GlyCys: 0.883 ± 0.034	3.83GlyAsp: 3.83 ± 0.063	4.714GlyGlu: 4.714 ± 0.074	3.207GlyPhe: 3.207 ± 0.071	5.778GlyGly: 5.778 ± 0.101	1.943GlyHis: 1.943 ± 0.051	4.965GlyIle: 4.965 ± 0.078	4.285GlyLys: 4.285 ± 0.078	7.905GlyLeu: 7.905 ± 0.101	2.602GlyMet: 2.602 ± 0.058	2.654GlyAsn: 2.654 ± 0.061	2.172GlyPro: 2.172 ± 0.047	2.675GlyGln: 2.675 ± 0.06	4.482GlyArg: 4.482 ± 0.074	4.134GlySer: 4.134 ± 0.072	3.807GlyThr: 3.807 ± 0.079	5.947GlyVal: 5.947 ± 0.097	1.261GlyTrp: 1.261 ± 0.04	2.508GlyTyr: 2.508 ± 0.051	0.0GlyXaa: 0.0 ± 0.0
His	2.804HisAla: 2.804 ± 0.062	0.286HisCys: 0.286 ± 0.018	1.36HisAsp: 1.36 ± 0.044	1.372HisGlu: 1.372 ± 0.039	1.064HisPhe: 1.064 ± 0.036	2.128HisGly: 2.128 ± 0.054	0.819HisHis: 0.819 ± 0.03	1.267HisIle: 1.267 ± 0.043	0.818HisLys: 0.818 ± 0.029	2.507HisLeu: 2.507 ± 0.045	0.58HisMet: 0.58 ± 0.023	0.715HisAsn: 0.715 ± 0.029	1.591HisPro: 1.591 ± 0.046	0.796HisGln: 0.796 ± 0.032	1.434HisArg: 1.434 ± 0.04	1.164HisSer: 1.164 ± 0.036	1.091HisThr: 1.091 ± 0.034	1.544HisVal: 1.544 ± 0.047	0.35HisTrp: 0.35 ± 0.017	0.842HisTyr: 0.842 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	6.875IleAla: 6.875 ± 0.091	0.558IleCys: 0.558 ± 0.029	3.51IleAsp: 3.51 ± 0.068	3.821IleGlu: 3.821 ± 0.078	1.892IlePhe: 1.892 ± 0.054	4.85IleGly: 4.85 ± 0.079	1.296IleHis: 1.296 ± 0.039	2.527IleIle: 2.527 ± 0.061	2.267IleLys: 2.267 ± 0.055	4.975IleLeu: 4.975 ± 0.09	1.158IleMet: 1.158 ± 0.038	1.954IleAsn: 1.954 ± 0.049	2.829IlePro: 2.829 ± 0.057	1.77IleGln: 1.77 ± 0.048	3.241IleArg: 3.241 ± 0.064	3.392IleSer: 3.392 ± 0.073	3.037IleThr: 3.037 ± 0.06	4.121IleVal: 4.121 ± 0.073	0.564IleTrp: 0.564 ± 0.026	1.381IleTyr: 1.381 ± 0.035	0.0IleXaa: 0.0 ± 0.0
Lys	4.217LysAla: 4.217 ± 0.08	0.265LysCys: 0.265 ± 0.018	1.957LysAsp: 1.957 ± 0.049	2.444LysGlu: 2.444 ± 0.058	1.306LysPhe: 1.306 ± 0.036	2.847LysGly: 2.847 ± 0.064	1.026LysHis: 1.026 ± 0.034	2.427LysIle: 2.427 ± 0.054	2.011LysLys: 2.011 ± 0.062	4.675LysLeu: 4.675 ± 0.086	1.161LysMet: 1.161 ± 0.038	1.546LysAsn: 1.546 ± 0.043	2.385LysPro: 2.385 ± 0.062	1.919LysGln: 1.919 ± 0.042	2.646LysArg: 2.646 ± 0.05	2.508LysSer: 2.508 ± 0.045	2.439LysThr: 2.439 ± 0.056	3.085LysVal: 3.085 ± 0.069	0.439LysTrp: 0.439 ± 0.022	0.978LysTyr: 0.978 ± 0.037	0.0LysXaa: 0.0 ± 0.0
Leu	12.526LeuAla: 12.526 ± 0.142	0.926LeuCys: 0.926 ± 0.033	5.915LeuAsp: 5.915 ± 0.083	6.34LeuGlu: 6.34 ± 0.107	3.899LeuPhe: 3.899 ± 0.075	8.072LeuGly: 8.072 ± 0.113	2.697LeuHis: 2.697 ± 0.056	5.488LeuIle: 5.488 ± 0.088	4.742LeuLys: 4.742 ± 0.077	11.763LeuLeu: 11.763 ± 0.155	2.694LeuMet: 2.694 ± 0.058	3.413LeuAsn: 3.413 ± 0.067	5.648LeuPro: 5.648 ± 0.081	4.104LeuGln: 4.104 ± 0.076	6.559LeuArg: 6.559 ± 0.098	6.261LeuSer: 6.261 ± 0.088	5.581LeuThr: 5.581 ± 0.086	6.956LeuVal: 6.956 ± 0.101	1.225LeuTrp: 1.225 ± 0.044	2.443LeuTyr: 2.443 ± 0.06	0.0LeuXaa: 0.0 ± 0.0
Met	2.91MetAla: 2.91 ± 0.06	0.146MetCys: 0.146 ± 0.012	1.374MetAsp: 1.374 ± 0.046	1.583MetGlu: 1.583 ± 0.04	0.84MetPhe: 0.84 ± 0.036	1.867MetGly: 1.867 ± 0.047	0.712MetHis: 0.712 ± 0.03	1.298MetIle: 1.298 ± 0.035	1.305MetLys: 1.305 ± 0.041	3.209MetLeu: 3.209 ± 0.057	0.742MetMet: 0.742 ± 0.033	1.008MetAsn: 1.008 ± 0.036	1.595MetPro: 1.595 ± 0.042	1.325MetGln: 1.325 ± 0.037	1.89MetArg: 1.89 ± 0.044	1.602MetSer: 1.602 ± 0.04	1.534MetThr: 1.534 ± 0.043	1.83MetVal: 1.83 ± 0.053	0.212MetTrp: 0.212 ± 0.018	0.478MetTyr: 0.478 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	3.454AsnAla: 3.454 ± 0.068	0.342AsnCys: 0.342 ± 0.021	1.661AsnAsp: 1.661 ± 0.041	1.767AsnGlu: 1.767 ± 0.048	1.196AsnPhe: 1.196 ± 0.038	2.722AsnGly: 2.722 ± 0.072	0.717AsnHis: 0.717 ± 0.026	2.017AsnIle: 2.017 ± 0.046	1.236AsnLys: 1.236 ± 0.041	3.477AsnLeu: 3.477 ± 0.076	0.851AsnMet: 0.851 ± 0.032	1.114AsnAsn: 1.114 ± 0.043	2.105AsnPro: 2.105 ± 0.05	1.093AsnGln: 1.093 ± 0.032	2.068AsnArg: 2.068 ± 0.044	1.622AsnSer: 1.622 ± 0.045	1.72AsnThr: 1.72 ± 0.046	2.269AsnVal: 2.269 ± 0.051	0.499AsnTrp: 0.499 ± 0.022	0.917AsnTyr: 0.917 ± 0.032	0.0AsnXaa: 0.0 ± 0.0
Pro	5.144ProAla: 5.144 ± 0.09	0.369ProCys: 0.369 ± 0.022	3.212ProAsp: 3.212 ± 0.064	3.893ProGlu: 3.893 ± 0.076	1.791ProPhe: 1.791 ± 0.044	3.742ProGly: 3.742 ± 0.065	1.102ProHis: 1.102 ± 0.039	2.169ProIle: 2.169 ± 0.057	1.752ProLys: 1.752 ± 0.052	4.77ProLeu: 4.77 ± 0.077	1.187ProMet: 1.187 ± 0.034	1.45ProAsn: 1.45 ± 0.042	2.036ProPro: 2.036 ± 0.053	2.024ProGln: 2.024 ± 0.05	2.303ProArg: 2.303 ± 0.059	2.365ProSer: 2.365 ± 0.049	2.049ProThr: 2.049 ± 0.05	3.698ProVal: 3.698 ± 0.067	0.626ProTrp: 0.626 ± 0.027	1.311ProTyr: 1.311 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	4.601GlnAla: 4.601 ± 0.087	0.29GlnCys: 0.29 ± 0.017	1.811GlnAsp: 1.811 ± 0.05	2.31GlnGlu: 2.31 ± 0.06	1.306GlnPhe: 1.306 ± 0.037	2.743GlnGly: 2.743 ± 0.052	1.031GlnHis: 1.031 ± 0.036	2.047GlnIle: 2.047 ± 0.052	1.651GlnLys: 1.651 ± 0.046	4.098GlnLeu: 4.098 ± 0.079	1.048GlnMet: 1.048 ± 0.035	1.144GlnAsn: 1.144 ± 0.036	2.03GlnPro: 2.03 ± 0.05	2.07GlnGln: 2.07 ± 0.059	2.635GlnArg: 2.635 ± 0.061	2.156GlnSer: 2.156 ± 0.054	1.866GlnThr: 1.866 ± 0.053	2.767GlnVal: 2.767 ± 0.053	0.508GlnTrp: 0.508 ± 0.021	1.026GlnTyr: 1.026 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	5.525ArgAla: 5.525 ± 0.099	0.495ArgCys: 0.495 ± 0.024	3.331ArgAsp: 3.331 ± 0.065	4.276ArgGlu: 4.276 ± 0.081	2.58ArgPhe: 2.58 ± 0.051	3.789ArgGly: 3.789 ± 0.069	1.727ArgHis: 1.727 ± 0.045	3.969ArgIle: 3.969 ± 0.074	2.649ArgLys: 2.649 ± 0.061	7.105ArgLeu: 7.105 ± 0.102	1.964ArgMet: 1.964 ± 0.048	2.292ArgAsn: 2.292 ± 0.049	2.522ArgPro: 2.522 ± 0.053	2.855ArgGln: 2.855 ± 0.063	3.902ArgArg: 3.902 ± 0.085	2.997ArgSer: 2.997 ± 0.063	2.683ArgThr: 2.683 ± 0.055	4.088ArgVal: 4.088 ± 0.069	0.87ArgTrp: 0.87 ± 0.032	2.033ArgTyr: 2.033 ± 0.05	0.0ArgXaa: 0.0 ± 0.0
Ser	5.557SerAla: 5.557 ± 0.087	0.534SerCys: 0.534 ± 0.026	2.849SerAsp: 2.849 ± 0.062	3.179SerGlu: 3.179 ± 0.07	2.154SerPhe: 2.154 ± 0.054	5.093SerGly: 5.093 ± 0.069	1.34SerHis: 1.34 ± 0.039	3.106SerIle: 3.106 ± 0.061	2.015SerLys: 2.015 ± 0.06	6.021SerLeu: 6.021 ± 0.09	1.521SerMet: 1.521 ± 0.043	1.73SerAsn: 1.73 ± 0.045	2.61SerPro: 2.61 ± 0.051	1.978SerGln: 1.978 ± 0.05	3.519SerArg: 3.519 ± 0.074	3.168SerSer: 3.168 ± 0.069	2.665SerThr: 2.665 ± 0.06	3.778SerVal: 3.778 ± 0.074	0.716SerTrp: 0.716 ± 0.028	1.45SerTyr: 1.45 ± 0.044	0.0SerXaa: 0.0 ± 0.0
Thr	5.207ThrAla: 5.207 ± 0.071	0.405ThrCys: 0.405 ± 0.023	2.645ThrAsp: 2.645 ± 0.062	2.829ThrGlu: 2.829 ± 0.052	1.854ThrPhe: 1.854 ± 0.042	4.29ThrGly: 4.29 ± 0.072	1.254ThrHis: 1.254 ± 0.037	2.504ThrIle: 2.504 ± 0.052	1.447ThrLys: 1.447 ± 0.041	6.34ThrLeu: 6.34 ± 0.096	1.157ThrMet: 1.157 ± 0.039	1.432ThrAsn: 1.432 ± 0.04	3.018ThrPro: 3.018 ± 0.054	1.827ThrGln: 1.827 ± 0.049	3.024ThrArg: 3.024 ± 0.061	2.634ThrSer: 2.634 ± 0.064	2.48ThrThr: 2.48 ± 0.056	3.859ThrVal: 3.859 ± 0.076	0.572ThrTrp: 0.572 ± 0.028	1.307ThrTyr: 1.307 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	7.652ValAla: 7.652 ± 0.089	0.698ValCys: 0.698 ± 0.028	3.712ValAsp: 3.712 ± 0.075	4.38ValGlu: 4.38 ± 0.065	2.695ValPhe: 2.695 ± 0.062	5.041ValGly: 5.041 ± 0.083	1.476ValHis: 1.476 ± 0.042	4.27ValIle: 4.27 ± 0.078	2.971ValLys: 2.971 ± 0.07	7.566ValLeu: 7.566 ± 0.104	2.037ValMet: 2.037 ± 0.052	2.461ValAsn: 2.461 ± 0.06	3.19ValPro: 3.19 ± 0.06	2.394ValGln: 2.394 ± 0.061	4.196ValArg: 4.196 ± 0.069	4.216ValSer: 4.216 ± 0.075	3.814ValThr: 3.814 ± 0.071	5.44ValVal: 5.44 ± 0.09	0.87ValTrp: 0.87 ± 0.035	1.701ValTyr: 1.701 ± 0.046	0.0ValXaa: 0.0 ± 0.0
Trp	1.015TrpAla: 1.015 ± 0.04	0.132TrpCys: 0.132 ± 0.012	0.604TrpAsp: 0.604 ± 0.026	0.656TrpGlu: 0.656 ± 0.027	0.508TrpPhe: 0.508 ± 0.024	0.805TrpGly: 0.805 ± 0.033	0.406TrpHis: 0.406 ± 0.024	0.635TrpIle: 0.635 ± 0.03	0.576TrpLys: 0.576 ± 0.027	1.936TrpLeu: 1.936 ± 0.056	0.404TrpMet: 0.404 ± 0.021	0.445TrpAsn: 0.445 ± 0.025	0.563TrpPro: 0.563 ± 0.026	0.758TrpGln: 0.758 ± 0.034	0.985TrpArg: 0.985 ± 0.036	0.669TrpSer: 0.669 ± 0.032	0.534TrpThr: 0.534 ± 0.026	0.939TrpVal: 0.939 ± 0.035	0.207TrpTrp: 0.207 ± 0.015	0.344TrpTyr: 0.344 ± 0.025	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.721TyrAla: 2.721 ± 0.058	0.307TyrCys: 0.307 ± 0.018	1.428TyrAsp: 1.428 ± 0.052	1.423TyrGlu: 1.423 ± 0.038	1.188TyrPhe: 1.188 ± 0.036	2.231TyrGly: 2.231 ± 0.051	0.633TyrHis: 0.633 ± 0.03	1.173TyrIle: 1.173 ± 0.032	0.97TyrLys: 0.97 ± 0.04	3.047TyrLeu: 3.047 ± 0.056	0.534TyrMet: 0.534 ± 0.024	0.828TyrAsn: 0.828 ± 0.029	1.318TyrPro: 1.318 ± 0.033	1.152TyrGln: 1.152 ± 0.041	2.049TyrArg: 2.049 ± 0.056	1.574TyrSer: 1.574 ± 0.05	1.315TyrThr: 1.315 ± 0.036	1.785TyrVal: 1.785 ± 0.043	0.428TyrTrp: 0.428 ± 0.023	0.882TyrTyr: 0.882 ± 0.036	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2816 proteins (889654 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---