Sclerotinia sclerotiorum partitivirus S
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C7ENF5|C7ENF5_9VIRU Putative coat protein OS=Sclerotinia sclerotiorum partitivirus S OX=659497 PE=4 SV=1
MM1 pKa = 7.55 SSRR4 pKa = 11.84 SARR7 pKa = 11.84 KK8 pKa = 9.7 VSTQEE13 pKa = 3.83 KK14 pKa = 9.23 TSSQSGKK21 pKa = 9.71 NRR23 pKa = 11.84 ASKK26 pKa = 10.79 KK27 pKa = 9.38 SGKK30 pKa = 9.85 KK31 pKa = 9.31 SAPPEE36 pKa = 4.06 YY37 pKa = 10.7 SVDD40 pKa = 3.84 TPSEE44 pKa = 4.01 QSDD47 pKa = 4.05 VEE49 pKa = 4.17 SDD51 pKa = 3.01 ILSEE55 pKa = 5.06 LEE57 pKa = 4.37 VSDD60 pKa = 5.54 ADD62 pKa = 3.94 DD63 pKa = 3.74 ASRR66 pKa = 11.84 PVPASKK72 pKa = 10.35 SKK74 pKa = 10.82 KK75 pKa = 8.12 GTTRR79 pKa = 11.84 NKK81 pKa = 10.3 KK82 pKa = 9.6 KK83 pKa = 9.57 EE84 pKa = 3.85 KK85 pKa = 10.4 SVASSAGSSIPPYY98 pKa = 10.95 MMMLTALNFFSLTEE112 pKa = 4.06 IEE114 pKa = 4.72 HH115 pKa = 5.12 VTISTYY121 pKa = 10.4 TPSCWSMYY129 pKa = 10.62 AVLDD133 pKa = 3.97 AMHH136 pKa = 7.32 DD137 pKa = 3.87 LVGDD141 pKa = 3.7 NASLRR146 pKa = 11.84 RR147 pKa = 11.84 FCPYY151 pKa = 10.49 YY152 pKa = 10.58 HH153 pKa = 6.48 VALSNIYY160 pKa = 9.99 YY161 pKa = 10.3 GIIFIIQVLRR171 pKa = 11.84 ANQVANNLSQADD183 pKa = 3.85 FQFLRR188 pKa = 11.84 FFEE191 pKa = 5.27 SNFALEE197 pKa = 4.59 EE198 pKa = 4.21 LPVAGPLVLFFQNLAAFKK216 pKa = 10.35 PDD218 pKa = 3.0 GNRR221 pKa = 11.84 FNWVVPHH228 pKa = 5.56 YY229 pKa = 10.99 NNYY232 pKa = 9.86 GPANGANSPRR242 pKa = 11.84 VNAQSTALPQLPQMISLLNLFGASNAALLTAMDD275 pKa = 4.39 TAGQWEE281 pKa = 5.12 PFTFAAGGTIAGFAYY296 pKa = 10.26 GAGFATDD303 pKa = 4.31 AAASDD308 pKa = 3.58 QTLRR312 pKa = 11.84 APGINVPWQHH322 pKa = 6.47 DD323 pKa = 3.73 TAIHH327 pKa = 6.29 RR328 pKa = 11.84 KK329 pKa = 8.82 LVPISRR335 pKa = 11.84 RR336 pKa = 11.84 MSVPTMNGAKK346 pKa = 10.11 DD347 pKa = 3.58 SPRR350 pKa = 11.84 TYY352 pKa = 10.52 CGLDD356 pKa = 3.47 GQFHH360 pKa = 6.91 WFHH363 pKa = 6.53 KK364 pKa = 10.81 AIGAVSQEE372 pKa = 3.77 AKK374 pKa = 10.45 YY375 pKa = 9.94 FAGSTTLANINPSTGPSAVVEE396 pKa = 4.42 TTVAVPAAAIPQATAWYY413 pKa = 9.08 EE414 pKa = 4.07 GYY416 pKa = 9.8 PFNLNLTVNTRR427 pKa = 11.84 VRR429 pKa = 11.84 SISEE433 pKa = 3.87 TDD435 pKa = 3.3 VKK437 pKa = 10.74 IGTFAATCNIITGGNFTRR455 pKa = 11.84 WNGSSVFAGDD465 pKa = 3.57 FFVAATNPINFEE477 pKa = 3.91 NPTEE481 pKa = 4.16 VNAIAMAQPTMTLSLYY497 pKa = 10.05 RR498 pKa = 11.84 PKK500 pKa = 10.82 GGEE503 pKa = 4.06 ANSDD507 pKa = 3.7 DD508 pKa = 3.79
Molecular weight: 54.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.531
IPC2_protein 6.561
IPC_protein 6.766
Toseland 6.839
ProMoST 6.883
Dawson 7.249
Bjellqvist 7.088
Wikipedia 7.22
Rodwell 7.234
Grimsley 6.956
Solomon 7.307
Lehninger 7.307
Nozaki 7.41
DTASelect 7.512
Thurlkill 7.541
EMBOSS 7.6
Sillero 7.629
Patrickios 4.24
IPC_peptide 7.307
IPC2_peptide 7.029
IPC2.peptide.svr19 7.065
Protein with the highest isoelectric point:
>tr|C7ENF5|C7ENF5_9VIRU Putative coat protein OS=Sclerotinia sclerotiorum partitivirus S OX=659497 PE=4 SV=1
MM1 pKa = 7.68 PALNLRR7 pKa = 11.84 YY8 pKa = 9.4 LFSISRR14 pKa = 11.84 SEE16 pKa = 3.85 MKK18 pKa = 10.3 KK19 pKa = 9.69 IRR21 pKa = 11.84 SSHH24 pKa = 5.18 TKK26 pKa = 9.07 VSPRR30 pKa = 11.84 EE31 pKa = 3.61 EE32 pKa = 3.76 ATRR35 pKa = 11.84 FSILKK40 pKa = 9.8 HH41 pKa = 6.48 AILKK45 pKa = 9.76 HH46 pKa = 5.58 GSVGLLNQVLLGKK59 pKa = 10.02 RR60 pKa = 11.84 RR61 pKa = 11.84 SDD63 pKa = 3.36 ASDD66 pKa = 2.95 EE67 pKa = 4.29 RR68 pKa = 11.84 LIQDD72 pKa = 3.18 FHH74 pKa = 8.13 EE75 pKa = 5.12 FEE77 pKa = 4.59 QPVHH81 pKa = 5.79 PVPRR85 pKa = 11.84 DD86 pKa = 3.36 KK87 pKa = 10.99 HH88 pKa = 4.88 YY89 pKa = 11.29 LRR91 pKa = 11.84 ALRR94 pKa = 11.84 VTEE97 pKa = 4.57 KK98 pKa = 10.77 LMKK101 pKa = 9.23 PAKK104 pKa = 8.33 TLHH107 pKa = 6.74 PISFPDD113 pKa = 3.35 LRR115 pKa = 11.84 YY116 pKa = 10.26 YY117 pKa = 10.29 PWTKK121 pKa = 10.41 NVSAEE126 pKa = 3.73 APYY129 pKa = 10.92 NFEE132 pKa = 4.16 KK133 pKa = 10.53 KK134 pKa = 10.23 YY135 pKa = 11.18 EE136 pKa = 3.92 EE137 pKa = 4.28 LLRR140 pKa = 11.84 DD141 pKa = 3.66 KK142 pKa = 11.01 QRR144 pKa = 11.84 LGEE147 pKa = 4.29 IEE149 pKa = 3.98 TATATFHH156 pKa = 6.47 NLEE159 pKa = 4.55 DD160 pKa = 5.21 EE161 pKa = 4.14 IFEE164 pKa = 4.69 DD165 pKa = 4.39 NRR167 pKa = 11.84 YY168 pKa = 9.74 LIHH171 pKa = 7.18 KK172 pKa = 9.06 IKK174 pKa = 10.88 EE175 pKa = 4.01 GDD177 pKa = 3.61 SQFWDD182 pKa = 3.25 KK183 pKa = 11.26 DD184 pKa = 3.75 GKK186 pKa = 9.32 PRR188 pKa = 11.84 PYY190 pKa = 11.05 YY191 pKa = 9.64 HH192 pKa = 6.36 TTLHH196 pKa = 6.06 ARR198 pKa = 11.84 AHH200 pKa = 5.21 VVGHH204 pKa = 6.56 EE205 pKa = 4.23 DD206 pKa = 3.15 ADD208 pKa = 4.06 KK209 pKa = 10.37 IRR211 pKa = 11.84 AVFGVPKK218 pKa = 10.18 LLLMAEE224 pKa = 4.52 NMFIWPLQAYY234 pKa = 8.11 YY235 pKa = 11.04 LNQDD239 pKa = 3.54 TTKK242 pKa = 10.59 HH243 pKa = 5.45 HH244 pKa = 6.57 LLWGNEE250 pKa = 3.5 IMKK253 pKa = 10.57 GGWKK257 pKa = 10.03 KK258 pKa = 10.68 LWGQLQNGRR267 pKa = 11.84 ISRR270 pKa = 11.84 TILSLDD276 pKa = 2.65 WSEE279 pKa = 3.76 FDD281 pKa = 4.32 KK282 pKa = 11.22 RR283 pKa = 11.84 ALHH286 pKa = 6.1 EE287 pKa = 4.76 VIDD290 pKa = 5.01 DD291 pKa = 3.69 VHH293 pKa = 8.26 SMWKK297 pKa = 9.05 SWFDD301 pKa = 3.42 FTHH304 pKa = 6.08 YY305 pKa = 10.45 EE306 pKa = 3.6 PTIFYY311 pKa = 9.59 EE312 pKa = 4.28 KK313 pKa = 11.19 GEE315 pKa = 4.02 IPEE318 pKa = 3.68 PHH320 pKa = 6.54 RR321 pKa = 11.84 RR322 pKa = 11.84 IEE324 pKa = 4.17 NLWIWMTDD332 pKa = 3.28 MVKK335 pKa = 9.96 HH336 pKa = 5.24 YY337 pKa = 9.92 PILQPDD343 pKa = 3.95 GKK345 pKa = 10.64 VYY347 pKa = 9.72 QWTRR351 pKa = 11.84 NGIASGFQQTQLLDD365 pKa = 3.61 SFVNMIMLLTVLSANGINIEE385 pKa = 5.4 HH386 pKa = 7.44 PDD388 pKa = 3.43 FWIKK392 pKa = 10.53 VQGDD396 pKa = 3.66 DD397 pKa = 3.6 SLISIVEE404 pKa = 3.83 RR405 pKa = 11.84 RR406 pKa = 11.84 FQMFGISYY414 pKa = 10.69 LDD416 pKa = 3.46 TLADD420 pKa = 3.6 LASYY424 pKa = 9.58 YY425 pKa = 10.79 FNAKK429 pKa = 10.29 LSVKK433 pKa = 10.17 KK434 pKa = 10.76 SFISDD439 pKa = 3.81 TPQGQYY445 pKa = 10.31 VLGYY449 pKa = 8.67 FNHH452 pKa = 6.15 YY453 pKa = 9.49 GIPYY457 pKa = 10.38 RR458 pKa = 11.84 LDD460 pKa = 4.73 DD461 pKa = 5.11 DD462 pKa = 5.05 LLSHH466 pKa = 6.86 LVFPEE471 pKa = 4.1 RR472 pKa = 11.84 PQRR475 pKa = 11.84 LEE477 pKa = 3.63 EE478 pKa = 4.02 TAASCVGIAMASMGCSKK495 pKa = 10.42 VVYY498 pKa = 9.41 SICDD502 pKa = 3.33 DD503 pKa = 3.88 AYY505 pKa = 9.72 TFITKK510 pKa = 7.67 TLRR513 pKa = 11.84 RR514 pKa = 11.84 PAKK517 pKa = 10.18 AGSLFWLEE525 pKa = 3.71 RR526 pKa = 11.84 AFGYY530 pKa = 9.98 EE531 pKa = 3.85 MPNIAKK537 pKa = 8.89 MPTFEE542 pKa = 4.01 EE543 pKa = 4.29 CLYY546 pKa = 10.9 ASYY549 pKa = 10.19 DD550 pKa = 3.36 IPVRR554 pKa = 11.84 TEE556 pKa = 3.48 NMKK559 pKa = 10.32 QRR561 pKa = 11.84 LWPTNAKK568 pKa = 9.76 AKK570 pKa = 10.34 NGFYY574 pKa = 10.55 FLRR577 pKa = 11.84 HH578 pKa = 5.46 LCC580 pKa = 3.92
Molecular weight: 68.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.348
IPC2_protein 7.702
IPC_protein 7.629
Toseland 7.732
ProMoST 8.156
Dawson 8.375
Bjellqvist 8.478
Wikipedia 8.361
Rodwell 8.404
Grimsley 7.834
Solomon 8.478
Lehninger 8.463
Nozaki 8.536
DTASelect 8.361
Thurlkill 8.419
EMBOSS 8.565
Sillero 8.639
Patrickios 4.368
IPC_peptide 8.463
IPC2_peptide 7.395
IPC2.peptide.svr19 7.547
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1088
508
580
544.0
61.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.824 ± 2.218
0.827 ± 0.026
5.055 ± 0.604
5.239 ± 0.854
5.331 ± 0.119
5.515 ± 0.515
2.941 ± 0.896
5.423 ± 0.587
5.607 ± 0.966
8.272 ± 1.294
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.665 ± 0.199
5.055 ± 1.074
5.607 ± 0.454
3.493 ± 0.033
5.055 ± 0.733
7.813 ± 1.332
5.974 ± 0.859
5.239 ± 0.566
2.022 ± 0.422
4.044 ± 0.587
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here