Amino acid dipepetide frequency for Streptomyces phage Intolerant

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.798AlaAla: 15.798 ± 1.393
0.904AlaCys: 0.904 ± 0.341
7.055AlaAsp: 7.055 ± 0.7
9.527AlaGlu: 9.527 ± 1.137
3.377AlaPhe: 3.377 ± 0.537
10.733AlaGly: 10.733 ± 0.97
1.809AlaHis: 1.809 ± 0.385
3.437AlaIle: 3.437 ± 0.545
8.623AlaLys: 8.623 ± 1.784
10.13AlaLeu: 10.13 ± 0.915
3.678AlaMet: 3.678 ± 0.419
2.593AlaAsn: 2.593 ± 0.376
5.427AlaPro: 5.427 ± 0.704
4.281AlaGln: 4.281 ± 0.799
8.321AlaArg: 8.321 ± 0.727
5.005AlaSer: 5.005 ± 0.65
6.512AlaThr: 6.512 ± 0.631
7.899AlaVal: 7.899 ± 0.75
2.593AlaTrp: 2.593 ± 0.48
3.015AlaTyr: 3.015 ± 0.472
0.0AlaXaa: 0.0 ± 0.0
Cys
1.025CysAla: 1.025 ± 0.233
0.0CysCys: 0.0 ± 0.0
0.965CysAsp: 0.965 ± 0.254
0.965CysGlu: 0.965 ± 0.281
0.181CysPhe: 0.181 ± 0.111
1.387CysGly: 1.387 ± 0.36
0.241CysHis: 0.241 ± 0.128
0.543CysIle: 0.543 ± 0.193
0.362CysLys: 0.362 ± 0.14
0.422CysLeu: 0.422 ± 0.192
0.301CysMet: 0.301 ± 0.152
0.06CysAsn: 0.06 ± 0.069
0.784CysPro: 0.784 ± 0.305
0.301CysGln: 0.301 ± 0.142
0.784CysArg: 0.784 ± 0.301
0.784CysSer: 0.784 ± 0.183
0.543CysThr: 0.543 ± 0.205
0.784CysVal: 0.784 ± 0.241
0.121CysTrp: 0.121 ± 0.097
0.181CysTyr: 0.181 ± 0.097
0.0CysXaa: 0.0 ± 0.0
Asp
7.899AspAla: 7.899 ± 0.822
0.482AspCys: 0.482 ± 0.227
4.04AspAsp: 4.04 ± 0.593
5.125AspGlu: 5.125 ± 0.71
2.713AspPhe: 2.713 ± 0.343
6.211AspGly: 6.211 ± 0.824
0.784AspHis: 0.784 ± 0.207
1.809AspIle: 1.809 ± 0.334
2.834AspLys: 2.834 ± 0.39
4.945AspLeu: 4.945 ± 0.636
1.688AspMet: 1.688 ± 0.321
1.206AspAsn: 1.206 ± 0.4
3.196AspPro: 3.196 ± 0.449
1.447AspGln: 1.447 ± 0.264
4.342AspArg: 4.342 ± 0.417
2.11AspSer: 2.11 ± 0.339
3.075AspThr: 3.075 ± 0.442
4.402AspVal: 4.402 ± 0.531
1.628AspTrp: 1.628 ± 0.303
1.447AspTyr: 1.447 ± 0.254
0.0AspXaa: 0.0 ± 0.0
Glu
7.176GluAla: 7.176 ± 0.774
1.025GluCys: 1.025 ± 0.292
4.342GluAsp: 4.342 ± 0.582
3.618GluGlu: 3.618 ± 0.551
2.171GluPhe: 2.171 ± 0.365
4.764GluGly: 4.764 ± 0.618
1.387GluHis: 1.387 ± 0.278
2.713GluIle: 2.713 ± 0.442
3.437GluLys: 3.437 ± 0.564
5.125GluLeu: 5.125 ± 0.633
1.507GluMet: 1.507 ± 0.293
1.568GluAsn: 1.568 ± 0.274
3.437GluPro: 3.437 ± 0.628
3.558GluGln: 3.558 ± 0.42
4.643GluArg: 4.643 ± 0.711
2.713GluSer: 2.713 ± 0.419
3.799GluThr: 3.799 ± 0.416
4.342GluVal: 4.342 ± 0.508
1.387GluTrp: 1.387 ± 0.329
1.025GluTyr: 1.025 ± 0.271
0.0GluXaa: 0.0 ± 0.0
Phe
2.352PheAla: 2.352 ± 0.366
0.482PheCys: 0.482 ± 0.165
1.809PheAsp: 1.809 ± 0.271
1.869PheGlu: 1.869 ± 0.373
0.904PhePhe: 0.904 ± 0.205
3.075PheGly: 3.075 ± 0.429
0.543PheHis: 0.543 ± 0.177
1.146PheIle: 1.146 ± 0.341
1.507PheLys: 1.507 ± 0.385
2.352PheLeu: 2.352 ± 0.412
0.784PheMet: 0.784 ± 0.196
1.146PheAsn: 1.146 ± 0.323
1.507PhePro: 1.507 ± 0.265
0.965PheGln: 0.965 ± 0.218
2.412PheArg: 2.412 ± 0.364
1.568PheSer: 1.568 ± 0.273
1.809PheThr: 1.809 ± 0.389
2.05PheVal: 2.05 ± 0.375
0.543PheTrp: 0.543 ± 0.17
0.482PheTyr: 0.482 ± 0.16
0.0PheXaa: 0.0 ± 0.0
Gly
9.467GlyAla: 9.467 ± 0.928
0.724GlyCys: 0.724 ± 0.23
5.186GlyAsp: 5.186 ± 0.656
5.789GlyGlu: 5.789 ± 0.508
2.894GlyPhe: 2.894 ± 0.302
9.286GlyGly: 9.286 ± 0.83
1.447GlyHis: 1.447 ± 0.266
3.497GlyIle: 3.497 ± 0.626
5.427GlyLys: 5.427 ± 0.654
6.392GlyLeu: 6.392 ± 0.635
2.412GlyMet: 2.412 ± 0.361
2.713GlyAsn: 2.713 ± 0.419
3.377GlyPro: 3.377 ± 0.489
3.377GlyGln: 3.377 ± 0.476
4.583GlyArg: 4.583 ± 0.538
5.427GlySer: 5.427 ± 0.629
5.728GlyThr: 5.728 ± 0.653
7.477GlyVal: 7.477 ± 0.863
3.075GlyTrp: 3.075 ± 0.551
3.316GlyTyr: 3.316 ± 0.482
0.0GlyXaa: 0.0 ± 0.0
His
2.352HisAla: 2.352 ± 0.336
0.181HisCys: 0.181 ± 0.095
0.663HisAsp: 0.663 ± 0.229
1.025HisGlu: 1.025 ± 0.246
0.543HisPhe: 0.543 ± 0.152
1.869HisGly: 1.869 ± 0.319
0.301HisHis: 0.301 ± 0.128
0.543HisIle: 0.543 ± 0.193
0.965HisLys: 0.965 ± 0.314
1.387HisLeu: 1.387 ± 0.319
0.362HisMet: 0.362 ± 0.146
0.663HisAsn: 0.663 ± 0.206
1.447HisPro: 1.447 ± 0.296
0.301HisGln: 0.301 ± 0.118
1.146HisArg: 1.146 ± 0.289
0.965HisSer: 0.965 ± 0.262
0.663HisThr: 0.663 ± 0.194
0.904HisVal: 0.904 ± 0.228
0.241HisTrp: 0.241 ± 0.129
0.724HisTyr: 0.724 ± 0.203
0.0HisXaa: 0.0 ± 0.0
Ile
4.462IleAla: 4.462 ± 0.592
0.543IleCys: 0.543 ± 0.258
2.593IleAsp: 2.593 ± 0.471
1.99IleGlu: 1.99 ± 0.331
1.206IlePhe: 1.206 ± 0.289
2.653IleGly: 2.653 ± 0.434
0.904IleHis: 0.904 ± 0.224
1.387IleIle: 1.387 ± 0.282
2.593IleLys: 2.593 ± 0.566
2.412IleLeu: 2.412 ± 0.35
0.965IleMet: 0.965 ± 0.241
1.628IleAsn: 1.628 ± 0.303
2.291IlePro: 2.291 ± 0.344
1.206IleGln: 1.206 ± 0.231
3.075IleArg: 3.075 ± 0.438
1.869IleSer: 1.869 ± 0.372
2.472IleThr: 2.472 ± 0.392
3.316IleVal: 3.316 ± 0.484
0.603IleTrp: 0.603 ± 0.174
1.085IleTyr: 1.085 ± 0.216
0.0IleXaa: 0.0 ± 0.0
Lys
9.407LysAla: 9.407 ± 2.613
0.301LysCys: 0.301 ± 0.174
2.533LysAsp: 2.533 ± 0.404
2.472LysGlu: 2.472 ± 0.378
1.025LysPhe: 1.025 ± 0.181
4.643LysGly: 4.643 ± 0.475
1.146LysHis: 1.146 ± 0.355
2.352LysIle: 2.352 ± 0.468
4.04LysLys: 4.04 ± 1.247
5.306LysLeu: 5.306 ± 0.523
1.869LysMet: 1.869 ± 0.274
1.447LysAsn: 1.447 ± 0.25
3.256LysPro: 3.256 ± 0.51
1.628LysGln: 1.628 ± 0.343
3.075LysArg: 3.075 ± 0.439
3.075LysSer: 3.075 ± 0.55
3.739LysThr: 3.739 ± 0.602
3.196LysVal: 3.196 ± 0.36
1.206LysTrp: 1.206 ± 0.282
1.085LysTyr: 1.085 ± 0.326
0.0LysXaa: 0.0 ± 0.0
Leu
9.949LeuAla: 9.949 ± 0.682
0.904LeuCys: 0.904 ± 0.23
5.005LeuAsp: 5.005 ± 0.653
4.583LeuGlu: 4.583 ± 0.752
1.447LeuPhe: 1.447 ± 0.328
6.874LeuGly: 6.874 ± 0.605
1.447LeuHis: 1.447 ± 0.358
3.015LeuIle: 3.015 ± 0.453
5.005LeuLys: 5.005 ± 0.681
4.703LeuLeu: 4.703 ± 0.717
1.688LeuMet: 1.688 ± 0.384
2.05LeuAsn: 2.05 ± 0.404
4.221LeuPro: 4.221 ± 0.517
2.05LeuGln: 2.05 ± 0.324
5.005LeuArg: 5.005 ± 0.592
3.859LeuSer: 3.859 ± 0.532
5.367LeuThr: 5.367 ± 0.683
3.799LeuVal: 3.799 ± 0.585
1.99LeuTrp: 1.99 ± 0.472
1.869LeuTyr: 1.869 ± 0.308
0.0LeuXaa: 0.0 ± 0.0
Met
2.834MetAla: 2.834 ± 0.444
0.181MetCys: 0.181 ± 0.113
1.568MetAsp: 1.568 ± 0.336
1.327MetGlu: 1.327 ± 0.325
0.784MetPhe: 0.784 ± 0.214
1.749MetGly: 1.749 ± 0.243
0.301MetHis: 0.301 ± 0.122
1.266MetIle: 1.266 ± 0.289
1.507MetLys: 1.507 ± 0.301
1.869MetLeu: 1.869 ± 0.309
0.482MetMet: 0.482 ± 0.154
0.543MetAsn: 0.543 ± 0.16
1.869MetPro: 1.869 ± 0.411
0.724MetGln: 0.724 ± 0.229
1.628MetArg: 1.628 ± 0.291
1.99MetSer: 1.99 ± 0.317
2.291MetThr: 2.291 ± 0.372
1.206MetVal: 1.206 ± 0.288
0.121MetTrp: 0.121 ± 0.085
0.543MetTyr: 0.543 ± 0.184
0.0MetXaa: 0.0 ± 0.0
Asn
3.859AsnAla: 3.859 ± 0.858
0.422AsnCys: 0.422 ± 0.206
1.628AsnAsp: 1.628 ± 0.32
1.568AsnGlu: 1.568 ± 0.321
0.844AsnPhe: 0.844 ± 0.236
3.136AsnGly: 3.136 ± 0.421
0.301AsnHis: 0.301 ± 0.126
0.904AsnIle: 0.904 ± 0.219
1.025AsnLys: 1.025 ± 0.249
2.593AsnLeu: 2.593 ± 0.453
0.663AsnMet: 0.663 ± 0.226
1.327AsnAsn: 1.327 ± 0.42
2.713AsnPro: 2.713 ± 0.442
1.266AsnGln: 1.266 ± 0.247
1.146AsnArg: 1.146 ± 0.303
0.965AsnSer: 0.965 ± 0.267
1.688AsnThr: 1.688 ± 0.355
1.507AsnVal: 1.507 ± 0.372
0.422AsnTrp: 0.422 ± 0.139
0.543AsnTyr: 0.543 ± 0.27
0.0AsnXaa: 0.0 ± 0.0
Pro
5.849ProAla: 5.849 ± 0.705
0.543ProCys: 0.543 ± 0.205
3.98ProAsp: 3.98 ± 0.595
4.522ProGlu: 4.522 ± 0.501
1.869ProPhe: 1.869 ± 0.323
5.909ProGly: 5.909 ± 0.743
1.146ProHis: 1.146 ± 0.275
1.568ProIle: 1.568 ± 0.301
3.678ProLys: 3.678 ± 0.492
3.196ProLeu: 3.196 ± 0.49
0.844ProMet: 0.844 ± 0.204
1.507ProAsn: 1.507 ± 0.248
2.834ProPro: 2.834 ± 0.442
1.447ProGln: 1.447 ± 0.2
3.377ProArg: 3.377 ± 0.531
2.653ProSer: 2.653 ± 0.433
3.136ProThr: 3.136 ± 0.388
4.402ProVal: 4.402 ± 0.628
1.266ProTrp: 1.266 ± 0.35
1.507ProTyr: 1.507 ± 0.239
0.0ProXaa: 0.0 ± 0.0
Gln
4.462GlnAla: 4.462 ± 0.687
0.301GlnCys: 0.301 ± 0.147
1.749GlnAsp: 1.749 ± 0.322
1.387GlnGlu: 1.387 ± 0.291
0.965GlnPhe: 0.965 ± 0.234
2.894GlnGly: 2.894 ± 0.562
1.025GlnHis: 1.025 ± 0.247
1.507GlnIle: 1.507 ± 0.381
2.533GlnLys: 2.533 ± 0.731
2.593GlnLeu: 2.593 ± 0.383
0.904GlnMet: 0.904 ± 0.279
1.085GlnAsn: 1.085 ± 0.271
1.688GlnPro: 1.688 ± 0.45
1.809GlnGln: 1.809 ± 0.385
2.291GlnArg: 2.291 ± 0.404
1.568GlnSer: 1.568 ± 0.328
1.869GlnThr: 1.869 ± 0.258
1.869GlnVal: 1.869 ± 0.295
0.663GlnTrp: 0.663 ± 0.2
0.603GlnTyr: 0.603 ± 0.209
0.0GlnXaa: 0.0 ± 0.0
Arg
7.236ArgAla: 7.236 ± 0.711
1.146ArgCys: 1.146 ± 0.335
3.075ArgAsp: 3.075 ± 0.559
5.065ArgGlu: 5.065 ± 0.784
1.93ArgPhe: 1.93 ± 0.454
3.919ArgGly: 3.919 ± 0.548
0.844ArgHis: 0.844 ± 0.21
2.713ArgIle: 2.713 ± 0.461
3.558ArgLys: 3.558 ± 0.537
4.583ArgLeu: 4.583 ± 0.551
1.93ArgMet: 1.93 ± 0.36
1.749ArgAsn: 1.749 ± 0.346
3.678ArgPro: 3.678 ± 0.606
2.412ArgGln: 2.412 ± 0.411
4.703ArgArg: 4.703 ± 0.746
2.894ArgSer: 2.894 ± 0.464
3.256ArgThr: 3.256 ± 0.475
4.462ArgVal: 4.462 ± 0.508
1.688ArgTrp: 1.688 ± 0.351
2.291ArgTyr: 2.291 ± 0.391
0.0ArgXaa: 0.0 ± 0.0
Ser
5.849SerAla: 5.849 ± 0.783
0.362SerCys: 0.362 ± 0.128
3.075SerAsp: 3.075 ± 0.431
3.075SerGlu: 3.075 ± 0.462
1.206SerPhe: 1.206 ± 0.232
6.633SerGly: 6.633 ± 0.708
0.663SerHis: 0.663 ± 0.175
2.472SerIle: 2.472 ± 0.452
2.593SerLys: 2.593 ± 0.466
3.98SerLeu: 3.98 ± 0.434
1.085SerMet: 1.085 ± 0.349
1.507SerAsn: 1.507 ± 0.321
2.834SerPro: 2.834 ± 0.472
1.628SerGln: 1.628 ± 0.31
2.291SerArg: 2.291 ± 0.402
2.231SerSer: 2.231 ± 0.512
2.593SerThr: 2.593 ± 0.345
3.739SerVal: 3.739 ± 0.423
1.146SerTrp: 1.146 ± 0.278
1.568SerTyr: 1.568 ± 0.426
0.0SerXaa: 0.0 ± 0.0
Thr
8.02ThrAla: 8.02 ± 0.596
0.844ThrCys: 0.844 ± 0.274
4.161ThrAsp: 4.161 ± 0.457
3.015ThrGlu: 3.015 ± 0.437
1.387ThrPhe: 1.387 ± 0.273
5.548ThrGly: 5.548 ± 0.552
1.085ThrHis: 1.085 ± 0.226
3.256ThrIle: 3.256 ± 0.518
2.05ThrLys: 2.05 ± 0.356
3.919ThrLeu: 3.919 ± 0.558
1.146ThrMet: 1.146 ± 0.232
1.507ThrAsn: 1.507 ± 0.311
4.402ThrPro: 4.402 ± 0.455
1.025ThrGln: 1.025 ± 0.206
2.955ThrArg: 2.955 ± 0.577
4.281ThrSer: 4.281 ± 0.585
3.437ThrThr: 3.437 ± 0.514
4.764ThrVal: 4.764 ± 0.542
1.447ThrTrp: 1.447 ± 0.304
1.93ThrTyr: 1.93 ± 0.453
0.0ThrXaa: 0.0 ± 0.0
Val
7.718ValAla: 7.718 ± 0.633
0.784ValCys: 0.784 ± 0.233
4.161ValAsp: 4.161 ± 0.534
4.1ValGlu: 4.1 ± 0.527
2.291ValPhe: 2.291 ± 0.357
5.728ValGly: 5.728 ± 0.832
1.146ValHis: 1.146 ± 0.282
2.894ValIle: 2.894 ± 0.474
3.015ValLys: 3.015 ± 0.471
5.065ValLeu: 5.065 ± 0.573
1.688ValMet: 1.688 ± 0.324
2.231ValAsn: 2.231 ± 0.375
4.462ValPro: 4.462 ± 0.524
2.171ValGln: 2.171 ± 0.384
4.281ValArg: 4.281 ± 0.483
3.678ValSer: 3.678 ± 0.443
4.221ValThr: 4.221 ± 0.556
5.367ValVal: 5.367 ± 0.851
1.809ValTrp: 1.809 ± 0.377
2.05ValTyr: 2.05 ± 0.328
0.0ValXaa: 0.0 ± 0.0
Trp
2.231TrpAla: 2.231 ± 0.36
0.362TrpCys: 0.362 ± 0.143
2.05TrpAsp: 2.05 ± 0.363
1.206TrpGlu: 1.206 ± 0.251
0.663TrpPhe: 0.663 ± 0.238
1.628TrpGly: 1.628 ± 0.292
0.482TrpHis: 0.482 ± 0.167
1.628TrpIle: 1.628 ± 0.311
1.327TrpLys: 1.327 ± 0.257
1.628TrpLeu: 1.628 ± 0.361
0.301TrpMet: 0.301 ± 0.143
1.025TrpAsn: 1.025 ± 0.303
0.784TrpPro: 0.784 ± 0.25
1.085TrpGln: 1.085 ± 0.248
1.146TrpArg: 1.146 ± 0.262
1.387TrpSer: 1.387 ± 0.318
1.809TrpThr: 1.809 ± 0.311
1.447TrpVal: 1.447 ± 0.3
0.482TrpTrp: 0.482 ± 0.223
0.422TrpTyr: 0.422 ± 0.147
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.774TyrAla: 2.774 ± 0.344
0.241TyrCys: 0.241 ± 0.119
1.809TyrAsp: 1.809 ± 0.275
1.507TyrGlu: 1.507 ± 0.295
0.904TyrPhe: 0.904 ± 0.225
2.955TyrGly: 2.955 ± 0.49
0.241TyrHis: 0.241 ± 0.136
0.603TyrIle: 0.603 ± 0.185
0.724TyrLys: 0.724 ± 0.161
2.231TyrLeu: 2.231 ± 0.365
0.422TyrMet: 0.422 ± 0.204
0.965TyrAsn: 0.965 ± 0.301
1.025TyrPro: 1.025 ± 0.367
1.085TyrGln: 1.085 ± 0.295
1.93TyrArg: 1.93 ± 0.339
1.507TyrSer: 1.507 ± 0.335
2.171TyrThr: 2.171 ± 0.419
1.93TyrVal: 1.93 ± 0.315
0.663TyrTrp: 0.663 ± 0.163
0.603TyrTyr: 0.603 ± 0.241
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 78 proteins (16585 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski