Amino acid dipepetide frequency for Hydrogenophaga sp. BPS33

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.326AlaAla: 16.326 ± 0.131
1.384AlaCys: 1.384 ± 0.027
6.383AlaAsp: 6.383 ± 0.061
6.418AlaGlu: 6.418 ± 0.06
4.092AlaPhe: 4.092 ± 0.045
10.154AlaGly: 10.154 ± 0.111
3.094AlaHis: 3.094 ± 0.041
5.128AlaIle: 5.128 ± 0.05
3.602AlaLys: 3.602 ± 0.059
15.018AlaLeu: 15.018 ± 0.105
3.634AlaMet: 3.634 ± 0.04
2.912AlaAsn: 2.912 ± 0.044
6.437AlaPro: 6.437 ± 0.075
6.383AlaGln: 6.383 ± 0.06
9.195AlaArg: 9.195 ± 0.083
7.104AlaSer: 7.104 ± 0.074
6.24AlaThr: 6.24 ± 0.063
9.281AlaVal: 9.281 ± 0.076
1.886AlaTrp: 1.886 ± 0.039
2.433AlaTyr: 2.433 ± 0.039
0.0AlaXaa: 0.0 ± 0.0
Cys
1.297CysAla: 1.297 ± 0.026
0.143CysCys: 0.143 ± 0.009
0.529CysAsp: 0.529 ± 0.017
0.558CysGlu: 0.558 ± 0.016
0.322CysPhe: 0.322 ± 0.014
0.991CysGly: 0.991 ± 0.023
0.267CysHis: 0.267 ± 0.012
0.413CysIle: 0.413 ± 0.013
0.24CysLys: 0.24 ± 0.012
0.867CysLeu: 0.867 ± 0.022
0.226CysMet: 0.226 ± 0.011
0.251CysAsn: 0.251 ± 0.012
0.48CysPro: 0.48 ± 0.017
0.263CysGln: 0.263 ± 0.012
0.58CysArg: 0.58 ± 0.019
0.533CysSer: 0.533 ± 0.017
0.534CysThr: 0.534 ± 0.015
0.761CysVal: 0.761 ± 0.02
0.114CysTrp: 0.114 ± 0.007
0.177CysTyr: 0.177 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
7.134AspAla: 7.134 ± 0.069
0.46AspCys: 0.46 ± 0.017
2.632AspAsp: 2.632 ± 0.045
3.022AspGlu: 3.022 ± 0.041
2.032AspPhe: 2.032 ± 0.035
4.631AspGly: 4.631 ± 0.053
1.251AspHis: 1.251 ± 0.03
2.405AspIle: 2.405 ± 0.042
1.538AspLys: 1.538 ± 0.035
5.425AspLeu: 5.425 ± 0.052
1.33AspMet: 1.33 ± 0.028
1.212AspAsn: 1.212 ± 0.025
2.968AspPro: 2.968 ± 0.043
1.622AspGln: 1.622 ± 0.028
3.259AspArg: 3.259 ± 0.045
2.116AspSer: 2.116 ± 0.042
2.641AspThr: 2.641 ± 0.037
4.358AspVal: 4.358 ± 0.053
0.878AspTrp: 0.878 ± 0.021
1.148AspTyr: 1.148 ± 0.028
0.0AspXaa: 0.0 ± 0.0
Glu
6.799GluAla: 6.799 ± 0.062
0.415GluCys: 0.415 ± 0.015
2.348GluAsp: 2.348 ± 0.04
2.514GluGlu: 2.514 ± 0.041
1.698GluPhe: 1.698 ± 0.032
3.858GluGly: 3.858 ± 0.048
1.477GluHis: 1.477 ± 0.033
2.427GluIle: 2.427 ± 0.038
1.875GluLys: 1.875 ± 0.032
5.891GluLeu: 5.891 ± 0.068
1.317GluMet: 1.317 ± 0.024
1.299GluAsn: 1.299 ± 0.024
2.508GluPro: 2.508 ± 0.037
2.584GluGln: 2.584 ± 0.044
4.832GluArg: 4.832 ± 0.065
2.497GluSer: 2.497 ± 0.039
2.559GluThr: 2.559 ± 0.039
4.037GluVal: 4.037 ± 0.051
0.73GluTrp: 0.73 ± 0.021
0.891GluTyr: 0.891 ± 0.022
0.0GluXaa: 0.0 ± 0.0
Phe
4.158PheAla: 4.158 ± 0.046
0.431PheCys: 0.431 ± 0.014
2.422PheAsp: 2.422 ± 0.037
2.18PheGlu: 2.18 ± 0.037
1.283PhePhe: 1.283 ± 0.027
3.177PheGly: 3.177 ± 0.044
0.763PheHis: 0.763 ± 0.019
1.327PheIle: 1.327 ± 0.028
1.119PheLys: 1.119 ± 0.023
2.878PheLeu: 2.878 ± 0.046
0.792PheMet: 0.792 ± 0.021
1.079PheAsn: 1.079 ± 0.025
1.435PhePro: 1.435 ± 0.027
1.085PheGln: 1.085 ± 0.022
1.813PheArg: 1.813 ± 0.033
2.058PheSer: 2.058 ± 0.038
1.882PheThr: 1.882 ± 0.032
2.693PheVal: 2.693 ± 0.041
0.458PheTrp: 0.458 ± 0.016
0.763PheTyr: 0.763 ± 0.018
0.0PheXaa: 0.0 ± 0.0
Gly
9.353GlyAla: 9.353 ± 0.101
0.816GlyCys: 0.816 ± 0.02
3.94GlyAsp: 3.94 ± 0.064
4.426GlyGlu: 4.426 ± 0.054
3.221GlyPhe: 3.221 ± 0.039
7.024GlyGly: 7.024 ± 0.11
2.141GlyHis: 2.141 ± 0.036
3.824GlyIle: 3.824 ± 0.043
3.084GlyLys: 3.084 ± 0.043
8.856GlyLeu: 8.856 ± 0.079
2.417GlyMet: 2.417 ± 0.034
2.144GlyAsn: 2.144 ± 0.056
3.276GlyPro: 3.276 ± 0.042
3.507GlyGln: 3.507 ± 0.044
5.404GlyArg: 5.404 ± 0.06
4.781GlySer: 4.781 ± 0.079
4.553GlyThr: 4.553 ± 0.052
6.884GlyVal: 6.884 ± 0.058
1.428GlyTrp: 1.428 ± 0.031
2.226GlyTyr: 2.226 ± 0.036
0.0GlyXaa: 0.0 ± 0.0
His
3.131HisAla: 3.131 ± 0.048
0.299HisCys: 0.299 ± 0.013
1.22HisAsp: 1.22 ± 0.026
1.256HisGlu: 1.256 ± 0.027
0.893HisPhe: 0.893 ± 0.022
2.262HisGly: 2.262 ± 0.039
0.725HisHis: 0.725 ± 0.02
1.058HisIle: 1.058 ± 0.023
0.631HisLys: 0.631 ± 0.018
2.559HisLeu: 2.559 ± 0.036
0.562HisMet: 0.562 ± 0.017
0.58HisAsn: 0.58 ± 0.017
1.635HisPro: 1.635 ± 0.033
0.831HisGln: 0.831 ± 0.022
1.665HisArg: 1.665 ± 0.034
1.109HisSer: 1.109 ± 0.024
1.319HisThr: 1.319 ± 0.026
1.853HisVal: 1.853 ± 0.034
0.441HisTrp: 0.441 ± 0.014
0.591HisTyr: 0.591 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
5.913IleAla: 5.913 ± 0.054
0.423IleCys: 0.423 ± 0.016
2.912IleAsp: 2.912 ± 0.04
3.009IleGlu: 3.009 ± 0.043
1.122IlePhe: 1.122 ± 0.025
3.987IleGly: 3.987 ± 0.047
0.897IleHis: 0.897 ± 0.023
1.228IleIle: 1.228 ± 0.028
1.331IleLys: 1.331 ± 0.031
3.016IleLeu: 3.016 ± 0.041
0.686IleMet: 0.686 ± 0.021
1.295IleAsn: 1.295 ± 0.031
1.993IlePro: 1.993 ± 0.032
1.399IleGln: 1.399 ± 0.025
2.498IleArg: 2.498 ± 0.037
2.184IleSer: 2.184 ± 0.037
2.458IleThr: 2.458 ± 0.041
3.443IleVal: 3.443 ± 0.044
0.444IleTrp: 0.444 ± 0.015
0.842IleTyr: 0.842 ± 0.022
0.0IleXaa: 0.0 ± 0.0
Lys
4.13LysAla: 4.13 ± 0.059
0.165LysCys: 0.165 ± 0.01
1.636LysAsp: 1.636 ± 0.034
1.423LysGlu: 1.423 ± 0.031
0.822LysPhe: 0.822 ± 0.022
2.439LysGly: 2.439 ± 0.035
0.655LysHis: 0.655 ± 0.017
1.332LysIle: 1.332 ± 0.029
1.284LysLys: 1.284 ± 0.035
3.245LysLeu: 3.245 ± 0.049
0.753LysMet: 0.753 ± 0.024
0.926LysAsn: 0.926 ± 0.021
2.089LysPro: 2.089 ± 0.038
1.128LysGln: 1.128 ± 0.025
2.203LysArg: 2.203 ± 0.036
1.759LysSer: 1.759 ± 0.035
1.99LysThr: 1.99 ± 0.041
2.413LysVal: 2.413 ± 0.037
0.344LysTrp: 0.344 ± 0.014
0.566LysTyr: 0.566 ± 0.016
0.0LysXaa: 0.0 ± 0.0
Leu
14.175LeuAla: 14.175 ± 0.101
1.09LeuCys: 1.09 ± 0.024
5.752LeuAsp: 5.752 ± 0.058
5.214LeuGlu: 5.214 ± 0.057
3.182LeuPhe: 3.182 ± 0.046
8.806LeuGly: 8.806 ± 0.068
2.511LeuHis: 2.511 ± 0.044
4.008LeuIle: 4.008 ± 0.055
3.595LeuLys: 3.595 ± 0.038
10.905LeuLeu: 10.905 ± 0.119
2.617LeuMet: 2.617 ± 0.042
2.953LeuAsn: 2.953 ± 0.039
6.086LeuPro: 6.086 ± 0.07
4.518LeuGln: 4.518 ± 0.057
7.775LeuArg: 7.775 ± 0.073
6.445LeuSer: 6.445 ± 0.055
5.141LeuThr: 5.141 ± 0.057
7.969LeuVal: 7.969 ± 0.08
1.329LeuTrp: 1.329 ± 0.031
1.919LeuTyr: 1.919 ± 0.032
0.0LeuXaa: 0.0 ± 0.0
Met
3.391MetAla: 3.391 ± 0.045
0.205MetCys: 0.205 ± 0.011
1.2MetAsp: 1.2 ± 0.024
1.126MetGlu: 1.126 ± 0.026
0.701MetPhe: 0.701 ± 0.02
2.176MetGly: 2.176 ± 0.039
0.571MetHis: 0.571 ± 0.017
0.832MetIle: 0.832 ± 0.022
1.072MetLys: 1.072 ± 0.026
2.548MetLeu: 2.548 ± 0.036
0.517MetMet: 0.517 ± 0.018
0.943MetAsn: 0.943 ± 0.02
1.619MetPro: 1.619 ± 0.027
1.083MetGln: 1.083 ± 0.027
1.778MetArg: 1.778 ± 0.028
1.632MetSer: 1.632 ± 0.031
1.552MetThr: 1.552 ± 0.028
1.903MetVal: 1.903 ± 0.035
0.225MetTrp: 0.225 ± 0.011
0.331MetTyr: 0.331 ± 0.012
0.0MetXaa: 0.0 ± 0.0
Asn
3.547AsnAla: 3.547 ± 0.046
0.233AsnCys: 0.233 ± 0.011
1.339AsnAsp: 1.339 ± 0.034
1.264AsnGlu: 1.264 ± 0.026
0.901AsnPhe: 0.901 ± 0.023
2.318AsnGly: 2.318 ± 0.045
0.574AsnHis: 0.574 ± 0.018
1.195AsnIle: 1.195 ± 0.028
0.796AsnLys: 0.796 ± 0.025
2.548AsnLeu: 2.548 ± 0.035
0.601AsnMet: 0.601 ± 0.017
0.743AsnAsn: 0.743 ± 0.026
1.897AsnPro: 1.897 ± 0.032
0.89AsnGln: 0.89 ± 0.022
1.718AsnArg: 1.718 ± 0.029
1.19AsnSer: 1.19 ± 0.03
1.559AsnThr: 1.559 ± 0.033
1.974AsnVal: 1.974 ± 0.038
0.375AsnTrp: 0.375 ± 0.014
0.577AsnTyr: 0.577 ± 0.018
0.0AsnXaa: 0.0 ± 0.0
Pro
6.867ProAla: 6.867 ± 0.077
0.376ProCys: 0.376 ± 0.015
3.17ProAsp: 3.17 ± 0.045
3.275ProGlu: 3.275 ± 0.047
1.838ProPhe: 1.838 ± 0.03
4.762ProGly: 4.762 ± 0.056
1.314ProHis: 1.314 ± 0.027
1.965ProIle: 1.965 ± 0.035
1.524ProLys: 1.524 ± 0.032
5.411ProLeu: 5.411 ± 0.064
1.499ProMet: 1.499 ± 0.025
1.46ProAsn: 1.46 ± 0.025
2.768ProPro: 2.768 ± 0.046
2.197ProGln: 2.197 ± 0.039
3.227ProArg: 3.227 ± 0.043
3.163ProSer: 3.163 ± 0.042
2.87ProThr: 2.87 ± 0.037
4.448ProVal: 4.448 ± 0.052
0.796ProTrp: 0.796 ± 0.02
1.197ProTyr: 1.197 ± 0.025
0.0ProXaa: 0.0 ± 0.0
Gln
6.043GlnAla: 6.043 ± 0.072
0.334GlnCys: 0.334 ± 0.012
1.695GlnAsp: 1.695 ± 0.03
1.671GlnGlu: 1.671 ± 0.027
1.151GlnPhe: 1.151 ± 0.026
3.322GlnGly: 3.322 ± 0.047
1.055GlnHis: 1.055 ± 0.025
1.695GlnIle: 1.695 ± 0.033
1.198GlnLys: 1.198 ± 0.027
4.197GlnLeu: 4.197 ± 0.05
1.04GlnMet: 1.04 ± 0.022
0.871GlnAsn: 0.871 ± 0.022
2.308GlnPro: 2.308 ± 0.032
1.977GlnGln: 1.977 ± 0.038
3.849GlnArg: 3.849 ± 0.052
2.078GlnSer: 2.078 ± 0.035
2.073GlnThr: 2.073 ± 0.029
3.058GlnVal: 3.058 ± 0.041
0.691GlnTrp: 0.691 ± 0.019
0.738GlnTyr: 0.738 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
8.119ArgAla: 8.119 ± 0.07
0.639ArgCys: 0.639 ± 0.021
3.662ArgAsp: 3.662 ± 0.048
4.383ArgGlu: 4.383 ± 0.058
2.763ArgPhe: 2.763 ± 0.041
4.689ArgGly: 4.689 ± 0.05
2.063ArgHis: 2.063 ± 0.037
3.28ArgIle: 3.28 ± 0.042
2.047ArgLys: 2.047 ± 0.035
7.966ArgLeu: 7.966 ± 0.079
1.991ArgMet: 1.991 ± 0.036
1.709ArgAsn: 1.709 ± 0.033
3.306ArgPro: 3.306 ± 0.044
3.038ArgGln: 3.038 ± 0.043
4.99ArgArg: 4.99 ± 0.067
3.803ArgSer: 3.803 ± 0.045
3.486ArgThr: 3.486 ± 0.044
5.37ArgVal: 5.37 ± 0.058
1.239ArgTrp: 1.239 ± 0.028
1.698ArgTyr: 1.698 ± 0.03
0.0ArgXaa: 0.0 ± 0.0
Ser
6.843SerAla: 6.843 ± 0.073
0.441SerCys: 0.441 ± 0.015
2.697SerAsp: 2.697 ± 0.038
2.665SerGlu: 2.665 ± 0.038
1.964SerPhe: 1.964 ± 0.037
5.304SerGly: 5.304 ± 0.061
1.348SerHis: 1.348 ± 0.029
2.327SerIle: 2.327 ± 0.037
1.549SerLys: 1.549 ± 0.028
5.726SerLeu: 5.726 ± 0.064
1.443SerMet: 1.443 ± 0.027
1.477SerAsn: 1.477 ± 0.034
3.142SerPro: 3.142 ± 0.042
1.929SerGln: 1.929 ± 0.029
3.643SerArg: 3.643 ± 0.046
3.267SerSer: 3.267 ± 0.058
3.135SerThr: 3.135 ± 0.046
4.198SerVal: 4.198 ± 0.055
0.725SerTrp: 0.725 ± 0.018
1.156SerTyr: 1.156 ± 0.025
0.0SerXaa: 0.0 ± 0.0
Thr
6.236ThrAla: 6.236 ± 0.067
0.456ThrCys: 0.456 ± 0.019
2.566ThrAsp: 2.566 ± 0.034
2.429ThrGlu: 2.429 ± 0.039
1.734ThrPhe: 1.734 ± 0.027
4.65ThrGly: 4.65 ± 0.058
1.301ThrHis: 1.301 ± 0.027
2.026ThrIle: 2.026 ± 0.034
1.222ThrLys: 1.222 ± 0.03
6.305ThrLeu: 6.305 ± 0.067
1.203ThrMet: 1.203 ± 0.024
1.188ThrAsn: 1.188 ± 0.027
3.882ThrPro: 3.882 ± 0.045
2.092ThrGln: 2.092 ± 0.033
3.429ThrArg: 3.429 ± 0.043
2.954ThrSer: 2.954 ± 0.042
3.162ThrThr: 3.162 ± 0.057
4.418ThrVal: 4.418 ± 0.055
0.699ThrTrp: 0.699 ± 0.021
1.061ThrTyr: 1.061 ± 0.027
0.0ThrXaa: 0.0 ± 0.0
Val
9.737ValAla: 9.737 ± 0.078
0.81ValCys: 0.81 ± 0.022
4.227ValAsp: 4.227 ± 0.046
4.111ValGlu: 4.111 ± 0.044
2.778ValPhe: 2.778 ± 0.039
5.836ValGly: 5.836 ± 0.056
1.774ValHis: 1.774 ± 0.032
3.299ValIle: 3.299 ± 0.043
2.44ValLys: 2.44 ± 0.04
8.529ValLeu: 8.529 ± 0.085
1.955ValMet: 1.955 ± 0.032
2.23ValAsn: 2.23 ± 0.039
4.38ValPro: 4.38 ± 0.051
3.075ValGln: 3.075 ± 0.041
5.574ValArg: 5.574 ± 0.054
4.383ValSer: 4.383 ± 0.062
3.947ValThr: 3.947 ± 0.054
6.894ValVal: 6.894 ± 0.073
1.09ValTrp: 1.09 ± 0.024
1.491ValTyr: 1.491 ± 0.026
0.0ValXaa: 0.0 ± 0.0
Trp
1.417TrpAla: 1.417 ± 0.03
0.17TrpCys: 0.17 ± 0.009
0.615TrpAsp: 0.615 ± 0.016
0.538TrpGlu: 0.538 ± 0.017
0.508TrpPhe: 0.508 ± 0.018
1.0TrpGly: 1.0 ± 0.022
0.41TrpHis: 0.41 ± 0.016
0.627TrpIle: 0.627 ± 0.016
0.461TrpLys: 0.461 ± 0.015
1.934TrpLeu: 1.934 ± 0.034
0.439TrpMet: 0.439 ± 0.015
0.412TrpAsn: 0.412 ± 0.013
0.799TrpPro: 0.799 ± 0.02
0.691TrpGln: 0.691 ± 0.021
1.242TrpArg: 1.242 ± 0.025
0.895TrpSer: 0.895 ± 0.022
0.729TrpThr: 0.729 ± 0.017
1.054TrpVal: 1.054 ± 0.025
0.279TrpTrp: 0.279 ± 0.012
0.27TrpTyr: 0.27 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.49TyrAla: 2.49 ± 0.034
0.253TyrCys: 0.253 ± 0.01
1.116TyrAsp: 1.116 ± 0.028
1.074TyrGlu: 1.074 ± 0.026
0.792TyrPhe: 0.792 ± 0.023
1.92TyrGly: 1.92 ± 0.031
0.427TyrHis: 0.427 ± 0.017
0.684TyrIle: 0.684 ± 0.019
0.692TyrLys: 0.692 ± 0.018
2.168TyrLeu: 2.168 ± 0.036
0.383TyrMet: 0.383 ± 0.013
0.555TyrAsn: 0.555 ± 0.018
1.116TyrPro: 1.116 ± 0.024
0.751TyrGln: 0.751 ± 0.024
1.571TyrArg: 1.571 ± 0.032
1.026TyrSer: 1.026 ± 0.024
1.182TyrThr: 1.182 ± 0.023
1.553TyrVal: 1.553 ± 0.028
0.322TyrTrp: 0.322 ± 0.015
0.455TyrTyr: 0.455 ± 0.017
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6055 proteins (2026464 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski