Burkholderia sp. (strain CCGE1003)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Burkholderia; unclassified Burkholderia

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5983 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E1TC63|E1TC63_BURSG OmpA/MotB domain protein OS=Burkholderia sp. (strain CCGE1003) OX=640512 GN=BC1003_1003 PE=4 SV=1
MM1 pKa = 8.03DD2 pKa = 3.32VTDD5 pKa = 3.79YY6 pKa = 11.25KK7 pKa = 11.3SWVCLICGWIYY18 pKa = 10.96NEE20 pKa = 4.45EE21 pKa = 3.98EE22 pKa = 4.14GLPDD26 pKa = 3.52EE27 pKa = 5.54GIPPGTRR34 pKa = 11.84FAAIPEE40 pKa = 4.07DD41 pKa = 4.0WRR43 pKa = 11.84CPLCDD48 pKa = 3.2VGKK51 pKa = 10.89AEE53 pKa = 4.06FAVVEE58 pKa = 4.78FF59 pKa = 4.61

Molecular weight: 6.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E1T9Z5|E1T9Z5_BURSG ABC transporter related protein OS=Burkholderia sp. (strain CCGE1003) OX=640512 GN=BC1003_1920 PE=4 SV=1
MM1 pKa = 7.18SLVRR5 pKa = 11.84VVRR8 pKa = 11.84VVRR11 pKa = 11.84VVRR14 pKa = 11.84VVRR17 pKa = 11.84VVRR20 pKa = 11.84VVRR23 pKa = 11.84VVRR26 pKa = 11.84VVRR29 pKa = 11.84VVRR32 pKa = 11.84VVRR35 pKa = 11.84VVRR38 pKa = 11.84VVHH41 pKa = 6.55AGPVKK46 pKa = 10.36HH47 pKa = 5.36VTHH50 pKa = 6.95RR51 pKa = 11.84SARR54 pKa = 11.84NTRR57 pKa = 11.84QTRR60 pKa = 11.84QKK62 pKa = 10.63GQPTT66 pKa = 3.53

Molecular weight: 7.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5983

0

5983

1998914

24

5768

334.1

36.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.097 ± 0.054

0.924 ± 0.01

5.23 ± 0.021

5.139 ± 0.029

3.64 ± 0.023

8.159 ± 0.037

2.31 ± 0.016

4.566 ± 0.023

3.016 ± 0.028

10.265 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.345 ± 0.014

2.866 ± 0.026

5.078 ± 0.025

3.699 ± 0.021

6.952 ± 0.036

5.852 ± 0.03

5.381 ± 0.03

7.743 ± 0.025

1.337 ± 0.013

2.4 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski