Bacillus phage Bam35c (Bacillus thuringiensis bacteriophage Bam35c)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes; Kalamavirales; Tectiviridae; Betatectivirus; Bacillus virus Bam35

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6X3V8|Q6X3V8_BP35C Uncharacterized protein OS=Bacillus phage Bam35c OX=236750 PE=4 SV=1
MM1 pKa = 7.66LNQFNLPEE9 pKa = 4.45TPGEE13 pKa = 4.3TKK15 pKa = 9.61QEE17 pKa = 4.08RR18 pKa = 11.84LEE20 pKa = 4.11QVADD24 pKa = 3.55QVLDD28 pKa = 3.77HH29 pKa = 6.97LPFPVKK35 pKa = 10.43VLAGNSINSFRR46 pKa = 11.84QALHH50 pKa = 6.71FEE52 pKa = 4.75DD53 pKa = 5.38FDD55 pKa = 3.93EE56 pKa = 5.41QIDD59 pKa = 3.79NGLEE63 pKa = 3.92KK64 pKa = 10.69LKK66 pKa = 10.9EE67 pKa = 4.16VIDD70 pKa = 3.78YY71 pKa = 9.14VQYY74 pKa = 9.34GTYY77 pKa = 9.94PSEE80 pKa = 4.0

Molecular weight: 9.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6X3T9|Q6X3T9_BP35C Lysozyme OS=Bacillus phage Bam35c OX=236750 PE=3 SV=1
MM1 pKa = 7.39VYY3 pKa = 10.41HH4 pKa = 6.5SVTMRR9 pKa = 11.84FVVLYY14 pKa = 10.29LKK16 pKa = 10.49MMLEE20 pKa = 4.68WKK22 pKa = 10.12RR23 pKa = 11.84VTSGNVEE30 pKa = 3.73TRR32 pKa = 11.84MRR34 pKa = 11.84QYY36 pKa = 11.73GLLII40 pKa = 4.13

Molecular weight: 4.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

32

0

32

4934

40

735

154.2

17.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.979 ± 0.424

0.73 ± 0.167

5.29 ± 0.312

7.884 ± 0.827

4.398 ± 0.237

7.337 ± 0.643

1.601 ± 0.16

5.432 ± 0.378

8.897 ± 0.753

8.046 ± 0.402

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.202 ± 0.247

4.743 ± 0.429

4.054 ± 0.343

3.648 ± 0.418

4.662 ± 0.33

5.797 ± 0.495

6.121 ± 0.366

6.79 ± 0.504

1.378 ± 0.201

4.013 ± 0.525
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski