Mycobacterium phage Trouble

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5Z510|S5Z510_9CAUD Minor tail subunit OS=Mycobacterium phage Trouble OX=1340825 GN=32 PE=4 SV=1
MM1 pKa = 7.61GFTLRR6 pKa = 11.84LFGIPVLSLEE16 pKa = 4.13ITGDD20 pKa = 3.64GSAEE24 pKa = 4.06EE25 pKa = 4.65YY26 pKa = 10.37ISLTGGSFEE35 pKa = 4.74LAPEE39 pKa = 3.98EE40 pKa = 4.15PEE42 pKa = 3.9YY43 pKa = 11.13DD44 pKa = 3.29EE45 pKa = 5.61EE46 pKa = 4.95YY47 pKa = 10.82YY48 pKa = 10.93EE49 pKa = 4.22EE50 pKa = 4.6DD51 pKa = 3.1RR52 pKa = 11.84SGFGFGVSS60 pKa = 3.28

Molecular weight: 6.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5Y4P9|S5Y4P9_9CAUD Uncharacterized protein OS=Mycobacterium phage Trouble OX=1340825 GN=48 PE=4 SV=1
MM1 pKa = 7.56SKK3 pKa = 10.41HH4 pKa = 5.59EE5 pKa = 4.26YY6 pKa = 10.39VRR8 pKa = 11.84FTADD12 pKa = 3.2PEE14 pKa = 4.41QFAHH18 pKa = 6.82LWVQGDD24 pKa = 3.74LARR27 pKa = 11.84RR28 pKa = 11.84LGLNRR33 pKa = 11.84VARR36 pKa = 11.84TGEE39 pKa = 4.01GRR41 pKa = 11.84HH42 pKa = 5.75RR43 pKa = 11.84LPDD46 pKa = 3.23VRR48 pKa = 11.84FSQEE52 pKa = 3.84LPGGTVYY59 pKa = 10.53WSVNRR64 pKa = 11.84KK65 pKa = 9.3GFFRR69 pKa = 11.84RR70 pKa = 11.84DD71 pKa = 3.17DD72 pKa = 3.85SLPSGWVQRR81 pKa = 11.84IYY83 pKa = 10.86PRR85 pKa = 11.84VATSFRR91 pKa = 11.84TAEE94 pKa = 3.85

Molecular weight: 10.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

94

0

94

16541

28

823

176.0

19.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.691 ± 0.421

0.707 ± 0.121

6.584 ± 0.221

6.336 ± 0.251

3.174 ± 0.148

8.814 ± 0.569

2.037 ± 0.179

4.849 ± 0.233

4.19 ± 0.248

8.573 ± 0.267

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.134 ± 0.132

3.168 ± 0.149

5.489 ± 0.194

3.253 ± 0.158

6.463 ± 0.37

6.227 ± 0.29

6.251 ± 0.28

7.231 ± 0.225

1.995 ± 0.138

2.835 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski