Penguin siadenovirus A
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A162HSM3|A0A162HSM3_9ADEN ORF8 OS=Penguin siadenovirus A OX=2005055 GN=CSPAdV-2_gp22 PE=4 SV=1
MM1 pKa = 7.92 DD2 pKa = 5.45 SEE4 pKa = 5.59 DD5 pKa = 3.91 EE6 pKa = 4.34 LEE8 pKa = 4.69 GPVMLPPLQVHH19 pKa = 7.12 RR20 pKa = 11.84 ISSTNRR26 pKa = 11.84 FTSGTVLDD34 pKa = 4.89 LPSMTDD40 pKa = 2.57 NCFVYY45 pKa = 10.54 NFKK48 pKa = 10.22 TPTGEE53 pKa = 4.16 TVFIEE58 pKa = 4.58 SNGQFKK64 pKa = 10.45 FSEE67 pKa = 4.65 YY68 pKa = 11.06 NFGTLFSGFNDD79 pKa = 3.24 VTLEE83 pKa = 3.93 MQLDD87 pKa = 3.62 FHH89 pKa = 8.11 YY90 pKa = 10.55 MFDD93 pKa = 4.64 CMLDD97 pKa = 3.9 FKK99 pKa = 10.68 WPNADD104 pKa = 3.76 LEE106 pKa = 4.53 YY107 pKa = 10.91 SVNVYY112 pKa = 7.73 TDD114 pKa = 3.21 RR115 pKa = 11.84 RR116 pKa = 11.84 IAIYY120 pKa = 10.4 DD121 pKa = 4.17 LSNADD126 pKa = 3.56 YY127 pKa = 10.24 WPTILAWFAWQCNSNGINFFQYY149 pKa = 10.43 VRR151 pKa = 11.84 LLNEE155 pKa = 4.68 DD156 pKa = 5.24 DD157 pKa = 4.07 EE158 pKa = 4.88 GCPYY162 pKa = 9.94 GTIFAA167 pKa = 5.2
Molecular weight: 19.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.856
IPC2_protein 3.973
IPC_protein 3.935
Toseland 3.732
ProMoST 4.101
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.846
Rodwell 3.77
Grimsley 3.643
Solomon 3.91
Lehninger 3.872
Nozaki 4.037
DTASelect 4.253
Thurlkill 3.783
EMBOSS 3.859
Sillero 4.062
Patrickios 0.439
IPC_peptide 3.91
IPC2_peptide 4.037
IPC2.peptide.svr19 3.935
Protein with the highest isoelectric point:
>tr|A0A162HSI2|A0A162HSI2_9ADEN Core protein pX OS=Penguin siadenovirus A OX=2005055 GN=CSPAdV-2_gp09 PE=4 SV=1
MM1 pKa = 7.22 TSIVYY6 pKa = 10.4 SPADD10 pKa = 3.3 SRR12 pKa = 11.84 GWGFGNSSMRR22 pKa = 11.84 DD23 pKa = 3.45 YY24 pKa = 11.51 YY25 pKa = 11.25 LIGGALEE32 pKa = 4.36 PSDD35 pKa = 3.52 IYY37 pKa = 9.13 TVRR40 pKa = 11.84 VRR42 pKa = 11.84 EE43 pKa = 3.76 HH44 pKa = 5.23 WRR46 pKa = 11.84 RR47 pKa = 11.84 KK48 pKa = 6.71 QRR50 pKa = 11.84 RR51 pKa = 11.84 KK52 pKa = 9.88 SAAVQATPVVVAPVRR67 pKa = 11.84 RR68 pKa = 11.84 PRR70 pKa = 11.84 RR71 pKa = 11.84 RR72 pKa = 11.84 RR73 pKa = 11.84 AVGVQVPSTRR83 pKa = 11.84 VLRR86 pKa = 11.84 SATRR90 pKa = 11.84 RR91 pKa = 11.84 LAPVVPALPAPAVVPALPAPVAPVAAAIPTVSAPVKK127 pKa = 9.93 RR128 pKa = 11.84 RR129 pKa = 11.84 RR130 pKa = 11.84 IGG132 pKa = 3.09
Molecular weight: 14.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.184
IPC2_protein 10.584
IPC_protein 11.915
Toseland 12.047
ProMoST 12.544
Dawson 12.047
Bjellqvist 12.047
Wikipedia 12.53
Rodwell 11.652
Grimsley 12.091
Solomon 12.544
Lehninger 12.442
Nozaki 12.047
DTASelect 12.047
Thurlkill 12.047
EMBOSS 12.544
Sillero 12.047
Patrickios 11.374
IPC_peptide 12.544
IPC2_peptide 11.535
IPC2.peptide.svr19 9.262
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
22
0
22
7646
57
1108
347.5
39.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.428 ± 0.381
2.106 ± 0.453
5.31 ± 0.261
5.467 ± 0.373
5.166 ± 0.217
4.551 ± 0.446
2.04 ± 0.255
6.762 ± 0.4
5.82 ± 0.693
9.639 ± 0.602
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.354 ± 0.184
6.539 ± 0.325
5.323 ± 0.319
4.578 ± 0.279
4.8 ± 0.481
7.219 ± 0.289
6.474 ± 0.439
5.31 ± 0.364
1.02 ± 0.099
4.094 ± 0.299
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here