Rotavirus G3

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus A

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I6WJT2|I6WJT2_9REOV Structural protein VP3 (Fragment) OS=Rotavirus G3 OX=73036 PE=4 SV=1
MM1 pKa = 7.26YY2 pKa = 10.17GIEE5 pKa = 4.26YY6 pKa = 7.25TTVLTFLISVILLNYY21 pKa = 8.65VLKK24 pKa = 10.73SLTRR28 pKa = 11.84IMDD31 pKa = 4.04FIIYY35 pKa = 9.89RR36 pKa = 11.84FLLIIVILSPLLNAQNYY53 pKa = 8.31GMNLPITGSMDD64 pKa = 3.38TPYY67 pKa = 10.73TNSTRR72 pKa = 11.84EE73 pKa = 4.09EE74 pKa = 4.15VFLTSTLCLYY84 pKa = 10.75YY85 pKa = 8.86PTEE88 pKa = 4.27AATEE92 pKa = 4.11INDD95 pKa = 3.85NSWKK99 pKa = 9.38DD100 pKa = 3.5TLSQLFLIKK109 pKa = 10.23GWPTGSIYY117 pKa = 10.71FKK119 pKa = 10.4DD120 pKa = 3.75YY121 pKa = 10.94TDD123 pKa = 3.33IASFSVDD130 pKa = 3.05PQLYY134 pKa = 9.83CDD136 pKa = 3.92YY137 pKa = 11.01NLVLMKK143 pKa = 10.45YY144 pKa = 10.21DD145 pKa = 3.39ATLQLDD151 pKa = 3.72MSEE154 pKa = 4.89LADD157 pKa = 3.93LLLNEE162 pKa = 4.55WLCNPMDD169 pKa = 3.29ITLYY173 pKa = 10.67YY174 pKa = 9.25YY175 pKa = 10.55QQTDD179 pKa = 3.57EE180 pKa = 4.17ANKK183 pKa = 8.08WISMGSSCTIKK194 pKa = 10.46VCPLNTQTLGIGCLTTDD211 pKa = 2.99TNTFEE216 pKa = 4.37EE217 pKa = 4.82VATAEE222 pKa = 4.34KK223 pKa = 10.74LVITDD228 pKa = 3.22VVDD231 pKa = 4.33GVNHH235 pKa = 6.78KK236 pKa = 11.16LNVTTNTCTIRR247 pKa = 11.84NCKK250 pKa = 9.83KK251 pKa = 10.18LGPRR255 pKa = 11.84EE256 pKa = 4.01NVAVIQVGGPDD267 pKa = 3.65VLDD270 pKa = 3.39ITADD274 pKa = 3.7PTTMPQTEE282 pKa = 3.54RR283 pKa = 11.84MMRR286 pKa = 11.84VNWKK290 pKa = 9.44KK291 pKa = 7.9WWQVFYY297 pKa = 10.66TIVDD301 pKa = 3.57YY302 pKa = 11.59VNQIVQAMSKK312 pKa = 10.19RR313 pKa = 11.84SRR315 pKa = 11.84SLNSAAFYY323 pKa = 11.0YY324 pKa = 10.27RR325 pKa = 11.84VV326 pKa = 3.19

Molecular weight: 37.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I6WA20|I6WA20_9REOV Outer capsid protein VP4 (Fragment) OS=Rotavirus G3 OX=73036 PE=3 SV=1
MM1 pKa = 7.81AEE3 pKa = 4.64LACFCYY9 pKa = 9.75PHH11 pKa = 7.6LEE13 pKa = 3.86NDD15 pKa = 3.51SYY17 pKa = 12.02KK18 pKa = 10.72FIPFNNLAIKK28 pKa = 10.73AMLTAKK34 pKa = 9.94VDD36 pKa = 3.61KK37 pKa = 10.98KK38 pKa = 11.38DD39 pKa = 3.01MDD41 pKa = 3.61KK42 pKa = 10.96FYY44 pKa = 11.34DD45 pKa = 3.78SIIYY49 pKa = 10.01GIAPPPQFKK58 pKa = 10.26KK59 pKa = 10.53RR60 pKa = 11.84YY61 pKa = 5.69NTNDD65 pKa = 2.88NSRR68 pKa = 11.84GMNFEE73 pKa = 4.56TIMFTKK79 pKa = 10.42VAMLICEE86 pKa = 4.59ALNSLKK92 pKa = 9.26VTQANVSNVLSRR104 pKa = 11.84VVSIRR109 pKa = 11.84HH110 pKa = 5.95LEE112 pKa = 3.77NLVISKK118 pKa = 9.17EE119 pKa = 4.07NPQDD123 pKa = 3.4ILFHH127 pKa = 7.08SKK129 pKa = 10.92DD130 pKa = 3.88LLLKK134 pKa = 9.6STLIAIGQSKK144 pKa = 10.2EE145 pKa = 3.61IEE147 pKa = 4.3TTVTAEE153 pKa = 3.64GGKK156 pKa = 9.58IVFQNAAFTMWKK168 pKa = 8.48LTYY171 pKa = 9.93LDD173 pKa = 4.02HH174 pKa = 8.02QLMPILDD181 pKa = 3.72QNFIEE186 pKa = 4.79YY187 pKa = 10.32KK188 pKa = 9.16VTLNEE193 pKa = 4.95DD194 pKa = 3.3KK195 pKa = 10.53PISDD199 pKa = 3.48GHH201 pKa = 6.27VKK203 pKa = 10.31EE204 pKa = 4.95LVAEE208 pKa = 4.51LRR210 pKa = 11.84WQYY213 pKa = 11.87NKK215 pKa = 10.27FAVITHH221 pKa = 6.31GKK223 pKa = 7.43GHH225 pKa = 5.73YY226 pKa = 9.48RR227 pKa = 11.84VVKK230 pKa = 8.49YY231 pKa = 10.96SSVANHH237 pKa = 7.16ADD239 pKa = 3.18RR240 pKa = 11.84VYY242 pKa = 10.85ATFKK246 pKa = 11.04SNVKK250 pKa = 9.52TGVNNDD256 pKa = 3.3FNLLDD261 pKa = 5.32QRR263 pKa = 11.84IIWQNWYY270 pKa = 10.72AFTSSMKK277 pKa = 10.28QGNTLDD283 pKa = 3.36VCKK286 pKa = 10.46RR287 pKa = 11.84LLFQKK292 pKa = 9.69MKK294 pKa = 10.49QEE296 pKa = 4.09KK297 pKa = 10.31NPFKK301 pKa = 10.91GLSTDD306 pKa = 2.99RR307 pKa = 11.84KK308 pKa = 8.2MDD310 pKa = 3.46EE311 pKa = 4.01VSQVGVV317 pKa = 3.32

Molecular weight: 36.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

3302

175

493

300.2

34.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.967 ± 0.399

1.514 ± 0.388

6.027 ± 0.489

6.057 ± 0.649

3.967 ± 0.387

3.301 ± 0.428

1.454 ± 0.24

7.45 ± 0.424

6.875 ± 0.764

9.419 ± 0.451

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.998 ± 0.238

7.571 ± 0.531

3.392 ± 0.42

3.816 ± 0.317

4.543 ± 0.349

7.753 ± 1.095

6.693 ± 0.794

5.815 ± 0.387

1.333 ± 0.195

5.058 ± 0.617
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski