Streptococcus satellite phage Javan646

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZTZ3|A0A4D5ZTZ3_9VIRU Bro-N domain-containing protein OS=Streptococcus satellite phage Javan646 OX=2558802 GN=JavanS646_0012 PE=4 SV=1
MM1 pKa = 8.1DD2 pKa = 4.13KK3 pKa = 10.75QDD5 pKa = 4.61VKK7 pKa = 9.3MTYY10 pKa = 9.56NNPMTFISSMVNAVKK25 pKa = 10.46HH26 pKa = 5.58IMNDD30 pKa = 3.66FNCDD34 pKa = 2.56KK35 pKa = 9.18TTALEE40 pKa = 4.17IYY42 pKa = 10.0KK43 pKa = 10.28LAYY46 pKa = 10.08QIANNQNMKK55 pKa = 10.32EE56 pKa = 4.15VTNMTNTNKK65 pKa = 8.99STAPMPLTEE74 pKa = 4.24LKK76 pKa = 10.53AWVEE80 pKa = 4.4EE81 pKa = 4.25TCEE84 pKa = 4.49LSEE87 pKa = 4.68LLQTKK92 pKa = 9.63DD93 pKa = 3.4YY94 pKa = 11.09KK95 pKa = 11.14SYY97 pKa = 10.96LPEE100 pKa = 4.1EE101 pKa = 4.19KK102 pKa = 10.1QEE104 pKa = 4.02EE105 pKa = 4.69FEE107 pKa = 4.48TIAFSVFNCLEE118 pKa = 4.11SFLMMLEE125 pKa = 4.26DD126 pKa = 5.51NEE128 pKa = 4.86LHH130 pKa = 5.74YY131 pKa = 10.58QPKK134 pKa = 9.84PIEE137 pKa = 4.32EE138 pKa = 4.24EE139 pKa = 3.89SDD141 pKa = 3.52LDD143 pKa = 3.81NQSNEE148 pKa = 3.55MAQYY152 pKa = 10.47QEE154 pKa = 4.05LAKK157 pKa = 10.51EE158 pKa = 4.21VEE160 pKa = 4.61DD161 pKa = 3.63NDD163 pKa = 3.64KK164 pKa = 10.3EE165 pKa = 4.07ARR167 pKa = 11.84EE168 pKa = 4.21IVCYY172 pKa = 10.15EE173 pKa = 3.89YY174 pKa = 11.07DD175 pKa = 3.84DD176 pKa = 5.08TIHH179 pKa = 6.98EE180 pKa = 4.72YY181 pKa = 9.73IDD183 pKa = 3.8KK184 pKa = 9.55RR185 pKa = 11.84TEE187 pKa = 3.8QLKK190 pKa = 9.5EE191 pKa = 3.5QATFEE196 pKa = 4.01EE197 pKa = 5.51LINKK201 pKa = 7.76VALYY205 pKa = 10.26EE206 pKa = 4.58SEE208 pKa = 4.11LLDD211 pKa = 3.57YY212 pKa = 11.28AEE214 pKa = 5.23RR215 pKa = 11.84LLSDD219 pKa = 3.74EE220 pKa = 4.79PLNADD225 pKa = 3.31SEE227 pKa = 4.81TAIRR231 pKa = 11.84TLDD234 pKa = 3.57MLDD237 pKa = 4.78DD238 pKa = 3.91EE239 pKa = 6.32AIDD242 pKa = 3.9LFKK245 pKa = 11.26SVDD248 pKa = 3.26IDD250 pKa = 3.57NEE252 pKa = 4.43YY253 pKa = 10.94QGLEE257 pKa = 4.14YY258 pKa = 11.29YY259 pKa = 8.15NTNLNKK265 pKa = 10.15EE266 pKa = 4.3DD267 pKa = 3.46

Molecular weight: 31.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZXV7|A0A4D5ZXV7_9VIRU Integrase/recombinase OS=Streptococcus satellite phage Javan646 OX=2558802 GN=JavanS646_0015 PE=3 SV=1
MM1 pKa = 7.27GRR3 pKa = 11.84YY4 pKa = 9.18NIHH7 pKa = 6.75PAQGGGHH14 pKa = 4.86YY15 pKa = 10.44HH16 pKa = 6.12NEE18 pKa = 3.29KK19 pKa = 9.9HH20 pKa = 6.12YY21 pKa = 10.81KK22 pKa = 9.61DD23 pKa = 3.59RR24 pKa = 11.84RR25 pKa = 11.84PTLEE29 pKa = 3.63QQEE32 pKa = 4.28EE33 pKa = 4.17QKK35 pKa = 10.79RR36 pKa = 11.84LKK38 pKa = 10.26KK39 pKa = 10.29LKK41 pKa = 8.85KK42 pKa = 9.14KK43 pKa = 10.03RR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84GYY48 pKa = 10.45

Molecular weight: 5.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15

0

15

2478

48

544

165.2

19.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.73 ± 0.422

0.646 ± 0.203

5.932 ± 0.605

8.676 ± 1.048

3.914 ± 0.499

4.076 ± 0.613

1.614 ± 0.343

7.869 ± 0.611

9.08 ± 0.54

10.412 ± 0.691

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.341 ± 0.316

6.376 ± 0.436

3.228 ± 0.507

4.883 ± 0.286

4.439 ± 0.611

4.358 ± 0.475

6.094 ± 0.442

4.52 ± 0.427

0.888 ± 0.122

4.923 ± 0.389
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski