Amino acid dipepetide frequency for Corynebacterium phage Stiles

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.522AlaAla: 20.522 ± 2.602
0.597AlaCys: 0.597 ± 0.181
10.075AlaAsp: 10.075 ± 1.041
9.776AlaGlu: 9.776 ± 1.044
2.687AlaPhe: 2.687 ± 0.443
11.343AlaGly: 11.343 ± 1.375
2.313AlaHis: 2.313 ± 0.468
5.97AlaIle: 5.97 ± 0.679
4.254AlaLys: 4.254 ± 0.646
9.851AlaLeu: 9.851 ± 0.976
3.881AlaMet: 3.881 ± 0.534
2.91AlaAsn: 2.91 ± 0.861
7.164AlaPro: 7.164 ± 0.677
5.075AlaGln: 5.075 ± 0.883
8.209AlaArg: 8.209 ± 1.032
5.522AlaSer: 5.522 ± 0.794
6.343AlaThr: 6.343 ± 0.667
7.985AlaVal: 7.985 ± 0.933
2.985AlaTrp: 2.985 ± 0.71
2.91AlaTyr: 2.91 ± 0.512
0.0AlaXaa: 0.0 ± 0.0
Cys
0.746CysAla: 0.746 ± 0.29
0.075CysCys: 0.075 ± 0.08
0.373CysAsp: 0.373 ± 0.154
0.299CysGlu: 0.299 ± 0.155
0.149CysPhe: 0.149 ± 0.094
0.448CysGly: 0.448 ± 0.197
0.149CysHis: 0.149 ± 0.1
0.224CysIle: 0.224 ± 0.11
0.224CysLys: 0.224 ± 0.13
0.373CysLeu: 0.373 ± 0.167
0.0CysMet: 0.0 ± 0.0
0.224CysAsn: 0.224 ± 0.128
0.224CysPro: 0.224 ± 0.138
0.149CysGln: 0.149 ± 0.102
0.448CysArg: 0.448 ± 0.18
0.448CysSer: 0.448 ± 0.188
0.522CysThr: 0.522 ± 0.215
0.224CysVal: 0.224 ± 0.176
0.373CysTrp: 0.373 ± 0.172
0.149CysTyr: 0.149 ± 0.116
0.0CysXaa: 0.0 ± 0.0
Asp
9.851AspAla: 9.851 ± 0.76
0.224AspCys: 0.224 ± 0.108
5.97AspAsp: 5.97 ± 0.869
4.925AspGlu: 4.925 ± 0.654
1.269AspPhe: 1.269 ± 0.283
7.761AspGly: 7.761 ± 0.831
1.493AspHis: 1.493 ± 0.281
2.836AspIle: 2.836 ± 0.469
1.642AspLys: 1.642 ± 0.537
5.597AspLeu: 5.597 ± 0.678
1.567AspMet: 1.567 ± 0.338
1.493AspAsn: 1.493 ± 0.355
3.731AspPro: 3.731 ± 0.708
2.463AspGln: 2.463 ± 0.387
3.731AspArg: 3.731 ± 0.468
2.388AspSer: 2.388 ± 0.46
4.03AspThr: 4.03 ± 0.796
5.373AspVal: 5.373 ± 0.731
1.642AspTrp: 1.642 ± 0.382
1.493AspTyr: 1.493 ± 0.312
0.0AspXaa: 0.0 ± 0.0
Glu
6.269GluAla: 6.269 ± 0.683
0.373GluCys: 0.373 ± 0.155
2.91GluAsp: 2.91 ± 0.419
4.179GluGlu: 4.179 ± 0.771
1.791GluPhe: 1.791 ± 0.316
3.433GluGly: 3.433 ± 0.605
2.164GluHis: 2.164 ± 0.557
3.507GluIle: 3.507 ± 0.512
2.388GluLys: 2.388 ± 0.449
5.373GluLeu: 5.373 ± 0.621
1.418GluMet: 1.418 ± 0.319
1.716GluAsn: 1.716 ± 0.349
4.478GluPro: 4.478 ± 0.892
3.507GluGln: 3.507 ± 0.405
4.179GluArg: 4.179 ± 0.726
3.806GluSer: 3.806 ± 0.372
3.358GluThr: 3.358 ± 0.557
5.149GluVal: 5.149 ± 0.561
1.194GluTrp: 1.194 ± 0.323
2.687GluTyr: 2.687 ± 0.508
0.0GluXaa: 0.0 ± 0.0
Phe
2.09PheAla: 2.09 ± 0.335
0.299PheCys: 0.299 ± 0.138
2.537PheAsp: 2.537 ± 0.405
1.493PheGlu: 1.493 ± 0.342
0.97PhePhe: 0.97 ± 0.47
3.134PheGly: 3.134 ± 0.439
0.373PheHis: 0.373 ± 0.172
1.493PheIle: 1.493 ± 0.302
0.746PheLys: 0.746 ± 0.275
1.866PheLeu: 1.866 ± 0.407
0.672PheMet: 0.672 ± 0.192
0.448PheAsn: 0.448 ± 0.166
0.821PhePro: 0.821 ± 0.202
0.672PheGln: 0.672 ± 0.219
1.493PheArg: 1.493 ± 0.429
1.269PheSer: 1.269 ± 0.441
1.716PheThr: 1.716 ± 0.333
1.567PheVal: 1.567 ± 0.324
0.597PheTrp: 0.597 ± 0.236
0.597PheTyr: 0.597 ± 0.192
0.0PheXaa: 0.0 ± 0.0
Gly
8.209GlyAla: 8.209 ± 1.216
0.522GlyCys: 0.522 ± 0.196
5.224GlyAsp: 5.224 ± 0.553
5.97GlyGlu: 5.97 ± 0.625
2.239GlyPhe: 2.239 ± 0.443
8.433GlyGly: 8.433 ± 1.35
2.463GlyHis: 2.463 ± 0.457
3.433GlyIle: 3.433 ± 0.812
3.955GlyLys: 3.955 ± 0.682
6.418GlyLeu: 6.418 ± 0.726
3.358GlyMet: 3.358 ± 0.521
2.463GlyAsn: 2.463 ± 0.454
4.328GlyPro: 4.328 ± 0.501
2.985GlyGln: 2.985 ± 0.421
6.119GlyArg: 6.119 ± 0.714
4.328GlySer: 4.328 ± 0.815
5.896GlyThr: 5.896 ± 0.863
6.791GlyVal: 6.791 ± 0.736
1.94GlyTrp: 1.94 ± 0.325
2.015GlyTyr: 2.015 ± 0.363
0.0GlyXaa: 0.0 ± 0.0
His
3.134HisAla: 3.134 ± 0.72
0.299HisCys: 0.299 ± 0.151
1.418HisAsp: 1.418 ± 0.341
1.418HisGlu: 1.418 ± 0.354
0.373HisPhe: 0.373 ± 0.159
1.716HisGly: 1.716 ± 0.303
1.045HisHis: 1.045 ± 0.333
1.269HisIle: 1.269 ± 0.269
0.373HisLys: 0.373 ± 0.142
2.612HisLeu: 2.612 ± 0.55
0.448HisMet: 0.448 ± 0.215
0.224HisAsn: 0.224 ± 0.145
1.642HisPro: 1.642 ± 0.368
1.119HisGln: 1.119 ± 0.329
1.045HisArg: 1.045 ± 0.284
0.746HisSer: 0.746 ± 0.214
1.194HisThr: 1.194 ± 0.32
2.388HisVal: 2.388 ± 0.512
0.299HisTrp: 0.299 ± 0.132
0.821HisTyr: 0.821 ± 0.222
0.0HisXaa: 0.0 ± 0.0
Ile
6.642IleAla: 6.642 ± 0.708
0.224IleCys: 0.224 ± 0.13
4.851IleAsp: 4.851 ± 0.667
2.239IleGlu: 2.239 ± 0.403
1.119IlePhe: 1.119 ± 0.292
4.403IleGly: 4.403 ± 1.138
1.194IleHis: 1.194 ± 0.31
2.612IleIle: 2.612 ± 0.43
1.119IleLys: 1.119 ± 0.305
3.284IleLeu: 3.284 ± 0.483
0.597IleMet: 0.597 ± 0.163
0.97IleAsn: 0.97 ± 0.272
2.463IlePro: 2.463 ± 0.367
1.866IleGln: 1.866 ± 0.439
3.507IleArg: 3.507 ± 0.506
2.313IleSer: 2.313 ± 0.49
3.582IleThr: 3.582 ± 0.78
2.91IleVal: 2.91 ± 0.392
0.448IleTrp: 0.448 ± 0.196
0.896IleTyr: 0.896 ± 0.293
0.0IleXaa: 0.0 ± 0.0
Lys
5.0LysAla: 5.0 ± 0.863
0.075LysCys: 0.075 ± 0.061
1.567LysAsp: 1.567 ± 0.331
1.418LysGlu: 1.418 ± 0.291
0.746LysPhe: 0.746 ± 0.265
2.09LysGly: 2.09 ± 0.409
0.672LysHis: 0.672 ± 0.209
0.97LysIle: 0.97 ± 0.394
1.194LysLys: 1.194 ± 0.354
3.955LysLeu: 3.955 ± 0.514
0.97LysMet: 0.97 ± 0.281
0.597LysAsn: 0.597 ± 0.212
1.269LysPro: 1.269 ± 0.36
1.418LysGln: 1.418 ± 0.295
1.94LysArg: 1.94 ± 0.439
2.239LysSer: 2.239 ± 0.517
2.612LysThr: 2.612 ± 0.414
2.239LysVal: 2.239 ± 0.422
0.522LysTrp: 0.522 ± 0.22
0.896LysTyr: 0.896 ± 0.217
0.0LysXaa: 0.0 ± 0.0
Leu
11.045LeuAla: 11.045 ± 0.922
0.373LeuCys: 0.373 ± 0.173
5.97LeuAsp: 5.97 ± 0.816
3.731LeuGlu: 3.731 ± 0.562
1.866LeuPhe: 1.866 ± 0.316
7.313LeuGly: 7.313 ± 0.744
1.791LeuHis: 1.791 ± 0.422
3.358LeuIle: 3.358 ± 0.531
2.537LeuLys: 2.537 ± 0.435
5.597LeuLeu: 5.597 ± 0.728
1.567LeuMet: 1.567 ± 0.285
2.015LeuAsn: 2.015 ± 0.383
5.224LeuPro: 5.224 ± 0.852
2.239LeuGln: 2.239 ± 0.341
6.493LeuArg: 6.493 ± 0.866
4.03LeuSer: 4.03 ± 0.628
5.224LeuThr: 5.224 ± 0.576
5.075LeuVal: 5.075 ± 0.648
1.716LeuTrp: 1.716 ± 0.365
1.716LeuTyr: 1.716 ± 0.435
0.0LeuXaa: 0.0 ± 0.0
Met
2.91MetAla: 2.91 ± 0.411
0.299MetCys: 0.299 ± 0.149
1.716MetAsp: 1.716 ± 0.328
1.642MetGlu: 1.642 ± 0.499
0.299MetPhe: 0.299 ± 0.148
2.388MetGly: 2.388 ± 0.5
0.448MetHis: 0.448 ± 0.21
0.97MetIle: 0.97 ± 0.256
0.97MetLys: 0.97 ± 0.268
1.418MetLeu: 1.418 ± 0.363
0.149MetMet: 0.149 ± 0.109
0.522MetAsn: 0.522 ± 0.197
1.716MetPro: 1.716 ± 0.285
0.821MetGln: 0.821 ± 0.225
1.94MetArg: 1.94 ± 0.446
1.493MetSer: 1.493 ± 0.32
2.836MetThr: 2.836 ± 0.559
1.194MetVal: 1.194 ± 0.301
0.448MetTrp: 0.448 ± 0.169
0.597MetTyr: 0.597 ± 0.251
0.0MetXaa: 0.0 ± 0.0
Asn
3.731AsnAla: 3.731 ± 0.571
0.0AsnCys: 0.0 ± 0.0
0.97AsnAsp: 0.97 ± 0.268
0.597AsnGlu: 0.597 ± 0.256
0.672AsnPhe: 0.672 ± 0.34
2.463AsnGly: 2.463 ± 0.558
0.821AsnHis: 0.821 ± 0.281
1.269AsnIle: 1.269 ± 0.325
0.224AsnLys: 0.224 ± 0.127
2.463AsnLeu: 2.463 ± 0.341
0.448AsnMet: 0.448 ± 0.168
0.299AsnAsn: 0.299 ± 0.15
2.09AsnPro: 2.09 ± 0.437
0.597AsnGln: 0.597 ± 0.231
2.164AsnArg: 2.164 ± 0.307
0.896AsnSer: 0.896 ± 0.251
0.896AsnThr: 0.896 ± 0.282
1.418AsnVal: 1.418 ± 0.333
0.224AsnTrp: 0.224 ± 0.128
0.448AsnTyr: 0.448 ± 0.183
0.0AsnXaa: 0.0 ± 0.0
Pro
6.866ProAla: 6.866 ± 0.73
0.224ProCys: 0.224 ± 0.129
5.299ProAsp: 5.299 ± 0.572
4.776ProGlu: 4.776 ± 0.76
1.716ProPhe: 1.716 ± 0.279
4.478ProGly: 4.478 ± 0.905
0.896ProHis: 0.896 ± 0.268
2.239ProIle: 2.239 ± 0.34
1.94ProLys: 1.94 ± 0.542
3.657ProLeu: 3.657 ± 0.58
1.343ProMet: 1.343 ± 0.371
1.119ProAsn: 1.119 ± 0.24
2.836ProPro: 2.836 ± 0.747
0.97ProGln: 0.97 ± 0.229
3.657ProArg: 3.657 ± 0.607
2.239ProSer: 2.239 ± 0.409
3.806ProThr: 3.806 ± 0.684
4.328ProVal: 4.328 ± 0.657
1.269ProTrp: 1.269 ± 0.326
0.896ProTyr: 0.896 ± 0.257
0.0ProXaa: 0.0 ± 0.0
Gln
5.0GlnAla: 5.0 ± 0.818
0.224GlnCys: 0.224 ± 0.106
0.746GlnAsp: 0.746 ± 0.242
1.716GlnGlu: 1.716 ± 0.408
0.97GlnPhe: 0.97 ± 0.311
2.91GlnGly: 2.91 ± 0.41
0.522GlnHis: 0.522 ± 0.22
1.343GlnIle: 1.343 ± 0.263
1.567GlnLys: 1.567 ± 0.319
4.03GlnLeu: 4.03 ± 0.429
1.269GlnMet: 1.269 ± 0.335
0.746GlnAsn: 0.746 ± 0.308
1.269GlnPro: 1.269 ± 0.333
1.493GlnGln: 1.493 ± 0.355
2.388GlnArg: 2.388 ± 0.3
1.418GlnSer: 1.418 ± 0.299
2.09GlnThr: 2.09 ± 0.351
3.358GlnVal: 3.358 ± 0.405
1.045GlnTrp: 1.045 ± 0.28
0.597GlnTyr: 0.597 ± 0.25
0.0GlnXaa: 0.0 ± 0.0
Arg
8.06ArgAla: 8.06 ± 0.798
0.597ArgCys: 0.597 ± 0.251
5.448ArgAsp: 5.448 ± 0.688
4.328ArgGlu: 4.328 ± 0.632
2.164ArgPhe: 2.164 ± 0.295
4.925ArgGly: 4.925 ± 0.673
1.716ArgHis: 1.716 ± 0.399
3.731ArgIle: 3.731 ± 0.537
2.836ArgLys: 2.836 ± 0.388
5.075ArgLeu: 5.075 ± 0.687
2.537ArgMet: 2.537 ± 0.36
1.269ArgAsn: 1.269 ± 0.322
3.507ArgPro: 3.507 ± 0.667
2.612ArgGln: 2.612 ± 0.501
6.343ArgArg: 6.343 ± 0.796
3.433ArgSer: 3.433 ± 0.524
3.881ArgThr: 3.881 ± 0.421
5.0ArgVal: 5.0 ± 0.613
2.164ArgTrp: 2.164 ± 0.356
1.343ArgTyr: 1.343 ± 0.334
0.0ArgXaa: 0.0 ± 0.0
Ser
5.075SerAla: 5.075 ± 0.829
0.149SerCys: 0.149 ± 0.153
2.463SerAsp: 2.463 ± 0.526
2.761SerGlu: 2.761 ± 0.448
2.164SerPhe: 2.164 ± 0.485
4.627SerGly: 4.627 ± 0.938
1.493SerHis: 1.493 ± 0.452
2.388SerIle: 2.388 ± 0.329
1.119SerLys: 1.119 ± 0.288
3.06SerLeu: 3.06 ± 0.557
1.418SerMet: 1.418 ± 0.313
1.119SerAsn: 1.119 ± 0.282
2.239SerPro: 2.239 ± 0.448
1.716SerGln: 1.716 ± 0.318
3.806SerArg: 3.806 ± 0.554
2.463SerSer: 2.463 ± 0.446
4.254SerThr: 4.254 ± 0.745
3.507SerVal: 3.507 ± 0.453
1.269SerTrp: 1.269 ± 0.202
1.045SerTyr: 1.045 ± 0.256
0.0SerXaa: 0.0 ± 0.0
Thr
8.507ThrAla: 8.507 ± 0.936
0.299ThrCys: 0.299 ± 0.197
3.433ThrAsp: 3.433 ± 0.715
3.955ThrGlu: 3.955 ± 0.589
1.194ThrPhe: 1.194 ± 0.301
6.045ThrGly: 6.045 ± 0.639
1.343ThrHis: 1.343 ± 0.319
4.179ThrIle: 4.179 ± 0.864
2.09ThrLys: 2.09 ± 0.438
5.224ThrLeu: 5.224 ± 0.67
1.194ThrMet: 1.194 ± 0.318
1.567ThrAsn: 1.567 ± 0.43
3.881ThrPro: 3.881 ± 0.72
1.567ThrGln: 1.567 ± 0.39
3.881ThrArg: 3.881 ± 0.556
3.06ThrSer: 3.06 ± 0.989
4.403ThrThr: 4.403 ± 0.76
5.149ThrVal: 5.149 ± 0.736
1.119ThrTrp: 1.119 ± 0.281
1.791ThrTyr: 1.791 ± 0.438
0.0ThrXaa: 0.0 ± 0.0
Val
10.373ValAla: 10.373 ± 0.843
0.448ValCys: 0.448 ± 0.191
5.075ValAsp: 5.075 ± 0.717
5.821ValGlu: 5.821 ± 0.681
1.567ValPhe: 1.567 ± 0.369
6.119ValGly: 6.119 ± 1.019
1.642ValHis: 1.642 ± 0.357
2.985ValIle: 2.985 ± 0.51
2.164ValLys: 2.164 ± 0.471
5.224ValLeu: 5.224 ± 0.866
0.97ValMet: 0.97 ± 0.319
1.791ValAsn: 1.791 ± 0.371
3.433ValPro: 3.433 ± 0.577
1.866ValGln: 1.866 ± 0.364
5.299ValArg: 5.299 ± 0.719
3.955ValSer: 3.955 ± 0.482
4.851ValThr: 4.851 ± 0.631
5.0ValVal: 5.0 ± 0.661
2.015ValTrp: 2.015 ± 0.351
1.269ValTyr: 1.269 ± 0.313
0.0ValXaa: 0.0 ± 0.0
Trp
3.433TrpAla: 3.433 ± 0.546
0.299TrpCys: 0.299 ± 0.145
1.567TrpAsp: 1.567 ± 0.37
1.567TrpGlu: 1.567 ± 0.352
0.373TrpPhe: 0.373 ± 0.178
1.269TrpGly: 1.269 ± 0.334
0.448TrpHis: 0.448 ± 0.18
1.269TrpIle: 1.269 ± 0.385
0.373TrpLys: 0.373 ± 0.153
2.164TrpLeu: 2.164 ± 0.457
0.522TrpMet: 0.522 ± 0.195
0.821TrpAsn: 0.821 ± 0.247
1.269TrpPro: 1.269 ± 0.336
0.821TrpGln: 0.821 ± 0.245
2.015TrpArg: 2.015 ± 0.276
0.746TrpSer: 0.746 ± 0.209
1.269TrpThr: 1.269 ± 0.353
1.343TrpVal: 1.343 ± 0.464
0.448TrpTrp: 0.448 ± 0.16
0.299TrpTyr: 0.299 ± 0.181
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.985TyrAla: 2.985 ± 0.389
0.224TyrCys: 0.224 ± 0.175
1.866TyrAsp: 1.866 ± 0.356
1.269TyrGlu: 1.269 ± 0.263
0.522TyrPhe: 0.522 ± 0.196
1.791TyrGly: 1.791 ± 0.43
0.597TyrHis: 0.597 ± 0.219
1.194TyrIle: 1.194 ± 0.346
0.672TyrLys: 0.672 ± 0.243
1.642TyrLeu: 1.642 ± 0.4
0.373TyrMet: 0.373 ± 0.178
0.373TyrAsn: 0.373 ± 0.183
0.97TyrPro: 0.97 ± 0.28
0.746TyrGln: 0.746 ± 0.264
2.313TyrArg: 2.313 ± 0.399
1.343TyrSer: 1.343 ± 0.354
1.119TyrThr: 1.119 ± 0.279
1.866TyrVal: 1.866 ± 0.357
0.597TyrTrp: 0.597 ± 0.257
0.075TyrTyr: 0.075 ± 0.071
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (13401 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski