Harrison Dam virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Sunrhavirus; Harrison sunrhavirus

Average proteome isoelectric point is 7.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0V1I6|A0A0A0V1I6_9RHAB Isoform of A0A0A0V5M3 C' protein OS=Harrison Dam virus OX=1569259 GN=P PE=4 SV=1
MM1 pKa = 7.49LSSKK5 pKa = 8.41TVEE8 pKa = 4.21NLTKK12 pKa = 10.62YY13 pKa = 10.3QEE15 pKa = 4.24AVQNEE20 pKa = 4.49PDD22 pKa = 4.26PSDD25 pKa = 3.87GDD27 pKa = 4.07LGDD30 pKa = 4.29SEE32 pKa = 6.13SMPQLEE38 pKa = 4.26LSGGVSRR45 pKa = 11.84EE46 pKa = 3.69SSAQLVEE53 pKa = 4.86FMQRR57 pKa = 11.84SNLADD62 pKa = 3.74GGACDD67 pKa = 5.52VPYY70 pKa = 10.1PWTADD75 pKa = 3.7SIHH78 pKa = 6.74NDD80 pKa = 2.58AFVNPEE86 pKa = 3.85GEE88 pKa = 4.17EE89 pKa = 4.04CRR91 pKa = 11.84DD92 pKa = 4.07LIDD95 pKa = 5.35EE96 pKa = 4.57SDD98 pKa = 3.67CGEE101 pKa = 4.12NSWSGRR107 pKa = 11.84RR108 pKa = 11.84SRR110 pKa = 11.84QIYY113 pKa = 8.57EE114 pKa = 3.7QGFEE118 pKa = 4.17DD119 pKa = 4.39GVRR122 pKa = 11.84EE123 pKa = 3.99VGRR126 pKa = 11.84QFRR129 pKa = 11.84MLQEE133 pKa = 3.84EE134 pKa = 4.86GFPFEE139 pKa = 4.28FDD141 pKa = 3.73YY142 pKa = 11.44VDD144 pKa = 3.76GRR146 pKa = 11.84IKK148 pKa = 10.57VKK150 pKa = 10.57KK151 pKa = 8.28ITCDD155 pKa = 3.16QRR157 pKa = 11.84ILIRR161 pKa = 11.84KK162 pKa = 8.51LIEE165 pKa = 4.31GGFFEE170 pKa = 5.6HH171 pKa = 7.34LSTDD175 pKa = 3.31MAVKK179 pKa = 10.09QKK181 pKa = 10.14SVKK184 pKa = 9.42PDD186 pKa = 3.18QPVAPLHH193 pKa = 6.13QILKK197 pKa = 8.67EE198 pKa = 4.14AKK200 pKa = 9.94KK201 pKa = 8.6PTQKK205 pKa = 10.98SMQNQEE211 pKa = 4.32EE212 pKa = 4.91KK213 pKa = 10.62FQQPLNTNSKK223 pKa = 8.83SAAMEE228 pKa = 3.92YY229 pKa = 10.39RR230 pKa = 11.84LSFCGDD236 pKa = 3.23DD237 pKa = 3.52TLTMEE242 pKa = 5.05EE243 pKa = 4.79FIEE246 pKa = 4.26KK247 pKa = 10.33ANRR250 pKa = 11.84GLHH253 pKa = 5.04ICKK256 pKa = 10.0DD257 pKa = 3.38GKK259 pKa = 10.55NRR261 pKa = 11.84FVDD264 pKa = 4.75LDD266 pKa = 3.99DD267 pKa = 4.92FCPQKK272 pKa = 10.34IRR274 pKa = 11.84EE275 pKa = 4.17RR276 pKa = 11.84KK277 pKa = 9.51ADD279 pKa = 4.01LLTLEE284 pKa = 4.29EE285 pKa = 4.61WLMCLYY291 pKa = 10.89

Molecular weight: 33.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0V7W9|A0A0A0V7W9_9RHAB Isoform of A0A0A0V5M3 C'' protein OS=Harrison Dam virus OX=1569259 GN=P PE=4 SV=1
MM1 pKa = 7.75RR2 pKa = 11.84SEE4 pKa = 5.14DD5 pKa = 4.03LDD7 pKa = 4.15SEE9 pKa = 4.35INRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84ILRR17 pKa = 11.84ASIHH21 pKa = 5.2RR22 pKa = 11.84HH23 pKa = 4.58GGEE26 pKa = 3.94AEE28 pKa = 3.82ISQTRR33 pKa = 11.84STSSPVASNPEE44 pKa = 3.96RR45 pKa = 11.84SQKK48 pKa = 10.83ANPEE52 pKa = 3.8IYY54 pKa = 10.35AEE56 pKa = 4.03PRR58 pKa = 11.84GKK60 pKa = 10.62VSTTFEE66 pKa = 4.32YY67 pKa = 10.66EE68 pKa = 3.97FKK70 pKa = 10.47IGRR73 pKa = 11.84DD74 pKa = 3.87GISSKK79 pKa = 10.75LLWGRR84 pKa = 11.84HH85 pKa = 4.33INNGGIYY92 pKa = 9.72RR93 pKa = 11.84KK94 pKa = 9.7SEE96 pKa = 3.5SWTTYY101 pKa = 10.25LL102 pKa = 5.1

Molecular weight: 11.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

4

9

3775

19

2074

419.4

48.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.841 ± 0.813

2.04 ± 0.177

5.669 ± 0.527

7.046 ± 0.451

4.795 ± 0.27

5.589 ± 0.49

2.093 ± 0.263

8.477 ± 0.639

8.185 ± 0.428

9.642 ± 0.544

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.358 ± 0.216

5.695 ± 0.43

3.656 ± 0.261

3.576 ± 0.407

5.113 ± 0.29

7.523 ± 0.828

4.874 ± 0.198

4.371 ± 0.29

1.854 ± 0.25

3.603 ± 0.425
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski