Torque teno virus (isolate Human/China/CT23F/2001) (TTV)
Average proteome isoelectric point is 8.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q8V7J0|CAPSD_TTVV6 Capsid protein OS=Torque teno virus (isolate Human/China/CT23F/2001) OX=687366 GN=ORF1 PE=3 SV=1
MM1 pKa = 7.69 PWRR4 pKa = 11.84 PPAHH8 pKa = 5.98 SVPGRR13 pKa = 11.84 EE14 pKa = 4.23 GQFYY18 pKa = 10.42 AATFHH23 pKa = 6.52 AHH25 pKa = 7.07 AAFCGCGGFIEE36 pKa = 4.92 HH37 pKa = 7.13 LNSIHH42 pKa = 6.81 PRR44 pKa = 11.84 FLGAGGPPPPPPALRR59 pKa = 11.84 RR60 pKa = 11.84 ALPAPEE66 pKa = 5.03 GPGGPPQHH74 pKa = 6.91 APPNPPPEE82 pKa = 5.29 GDD84 pKa = 3.39 HH85 pKa = 5.59 QPPRR89 pKa = 11.84 RR90 pKa = 11.84 GGGAGGAGDD99 pKa = 3.84 GHH101 pKa = 8.29 AGDD104 pKa = 4.26 GDD106 pKa = 3.72 AAEE109 pKa = 4.64 EE110 pKa = 4.15 YY111 pKa = 10.97 GPEE114 pKa = 4.51 DD115 pKa = 4.92 LDD117 pKa = 4.72 LLFAAAAEE125 pKa = 4.26 DD126 pKa = 4.55 DD127 pKa = 3.84 MM128 pKa = 7.94
Molecular weight: 13.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.999
IPC2_protein 5.016
IPC_protein 4.952
Toseland 5.067
ProMoST 5.169
Dawson 5.041
Bjellqvist 5.13
Wikipedia 4.952
Rodwell 4.965
Grimsley 5.041
Solomon 5.041
Lehninger 5.003
Nozaki 5.207
DTASelect 5.372
Thurlkill 5.105
EMBOSS 5.08
Sillero 5.27
Patrickios 3.694
IPC_peptide 5.041
IPC2_peptide 5.283
IPC2.peptide.svr19 5.14
Protein with the highest isoelectric point:
>sp|Q8V7J2|ORF24_TTVV6 Uncharacterized ORF2/4 protein OS=Torque teno virus (isolate Human/China/CT23F/2001) OX=687366 GN=ORF2/4 PE=4 SV=1
MM1 pKa = 7.69 PWRR4 pKa = 11.84 PPAHH8 pKa = 5.98 SVPGRR13 pKa = 11.84 EE14 pKa = 4.23 GQFYY18 pKa = 10.42 AATFHH23 pKa = 6.52 AHH25 pKa = 7.07 AAFCGCGGFIEE36 pKa = 4.92 HH37 pKa = 7.13 LNSIHH42 pKa = 6.81 PRR44 pKa = 11.84 FLGAGGPPPPPPALRR59 pKa = 11.84 RR60 pKa = 11.84 ALPAPEE66 pKa = 5.03 GPGGPPQHH74 pKa = 6.91 APPNPPPEE82 pKa = 5.29 GDD84 pKa = 3.39 HH85 pKa = 5.59 QPPRR89 pKa = 11.84 RR90 pKa = 11.84 GGGAGGAGDD99 pKa = 3.84 GHH101 pKa = 8.29 AGDD104 pKa = 4.26 GDD106 pKa = 3.72 AAEE109 pKa = 4.64 EE110 pKa = 4.15 YY111 pKa = 10.97 GPEE114 pKa = 4.51 DD115 pKa = 4.92 LDD117 pKa = 4.72 LLFAAAAEE125 pKa = 4.43 DD126 pKa = 4.04 DD127 pKa = 4.18 MSFKK131 pKa = 10.92 APSSRR136 pKa = 11.84 HH137 pKa = 3.71 QTRR140 pKa = 11.84 GPGRR144 pKa = 11.84 RR145 pKa = 11.84 AKK147 pKa = 10.26 KK148 pKa = 9.96 RR149 pKa = 11.84 LRR151 pKa = 11.84 FSPGSPRR158 pKa = 11.84 QPRR161 pKa = 11.84 LGGEE165 pKa = 4.38 SRR167 pKa = 11.84 RR168 pKa = 11.84 SPSPRR173 pKa = 11.84 RR174 pKa = 11.84 TSTPKK179 pKa = 10.0 RR180 pKa = 11.84 KK181 pKa = 9.8 RR182 pKa = 11.84 GATPQAAPAAKK193 pKa = 6.9 TTPASPQTRR202 pKa = 11.84 TPSPVRR208 pKa = 11.84 RR209 pKa = 11.84 RR210 pKa = 11.84 TQTEE214 pKa = 3.74 EE215 pKa = 4.3 GSPHH219 pKa = 6.97 RR220 pKa = 11.84 PPPYY224 pKa = 9.3 IAPPPIVEE232 pKa = 5.29 DD233 pKa = 3.62 LLFPNTQKK241 pKa = 10.86 KK242 pKa = 10.29 KK243 pKa = 10.52 KK244 pKa = 9.61 FSKK247 pKa = 10.52 FDD249 pKa = 3.46 WEE251 pKa = 4.46 TEE253 pKa = 4.0 AQLAACFDD261 pKa = 3.52 RR262 pKa = 11.84 PMRR265 pKa = 11.84 FFPSDD270 pKa = 3.37 LPTYY274 pKa = 9.5 PWLPKK279 pKa = 10.41 KK280 pKa = 9.29 PTTQTTFRR288 pKa = 11.84 VSFQLKK294 pKa = 10.05 APQQ297 pKa = 3.2
Molecular weight: 32.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.308
IPC2_protein 9.472
IPC_protein 9.926
Toseland 10.613
ProMoST 10.204
Dawson 10.687
Bjellqvist 10.35
Wikipedia 10.862
Rodwell 10.95
Grimsley 10.73
Solomon 10.789
Lehninger 10.76
Nozaki 10.584
DTASelect 10.35
Thurlkill 10.599
EMBOSS 10.994
Sillero 10.613
Patrickios 10.672
IPC_peptide 10.789
IPC2_peptide 9.194
IPC2.peptide.svr19 8.662
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1450
128
745
362.5
40.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.448 ± 2.001
1.448 ± 0.128
4.552 ± 0.26
4.897 ± 0.457
4.621 ± 0.329
8.069 ± 1.549
3.172 ± 0.477
2.69 ± 0.852
5.379 ± 0.724
7.034 ± 0.951
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.724 ± 0.269
2.207 ± 0.346
11.448 ± 2.45
4.414 ± 0.45
9.034 ± 0.81
6.966 ± 1.283
6.276 ± 0.875
3.034 ± 0.745
2.138 ± 0.765
3.448 ± 1.001
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here