Rhizopus stolonifer (Rhizopus nigricans)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Mucoromycota; Mucoromycotina; Mucoromycetes; Mucorales; Mucorineae; Rhizopodaceae; Rhizopus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13951 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A367KJ65|A0A367KJ65_RHIST GPI ethanolamine phosphate transferase 3 OS=Rhizopus stolonifer OX=4846 GN=CU098_006391 PE=3 SV=1
SS1 pKa = 7.19SDD3 pKa = 3.14SLTTVTSDD11 pKa = 2.73ILGVPTTYY19 pKa = 9.53VTTVPSSTTITSTYY33 pKa = 6.72TTLSDD38 pKa = 3.39VTFTSTTTVISDD50 pKa = 3.69TLSPSTTTGTSTYY63 pKa = 7.52TTLTTLSSTTTFTTTGANTIIARR86 pKa = 11.84FKK88 pKa = 10.96SFSIPSAATTTTTTLTTTSVSLQPTTSTTIALATVSTPTTSTVTSTSTVVLDD140 pKa = 3.61TLTITVLAPNAAVATIDD157 pKa = 3.27TTTIITTTTSPSITLTTTLTTTPTTTSTTTTLVVPATTTTVSTTPTTTTTTTTTVTSTTNVITATGTLTVPSTTTTTITTTSTSVVPAPITSTSVIVLVV256 pKa = 3.97

Molecular weight: 25.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A367IQD4|A0A367IQD4_RHIST Uncharacterized protein (Fragment) OS=Rhizopus stolonifer OX=4846 GN=CU098_007499 PE=4 SV=1
LL1 pKa = 7.46LLLLLTLSPQLQQIVALVLKK21 pKa = 8.58LQPNLLKK28 pKa = 10.84LQLLKK33 pKa = 10.81KK34 pKa = 9.45VTVLLKK40 pKa = 10.74LPSFSVQLNFLPQLPKK56 pKa = 11.09LLMAQLKK63 pKa = 10.41LLFLLPQLIPLTMVLLPQLIPLTMVLLPQLIPLIMALLLLLTRR106 pKa = 11.84RR107 pKa = 11.84SLVPKK112 pKa = 9.44PNQVNMLLPQLLTRR126 pKa = 11.84RR127 pKa = 11.84SLAQRR132 pKa = 11.84RR133 pKa = 11.84PLLLSTRR140 pKa = 11.84KK141 pKa = 10.14SLVLRR146 pKa = 11.84RR147 pKa = 11.84AKK149 pKa = 10.32QPLLLNTRR157 pKa = 11.84RR158 pKa = 11.84NLAPKK163 pKa = 9.87RR164 pKa = 11.84LSVLPAVLQRR174 pKa = 11.84PLPRR178 pKa = 11.84SFTLQSHH185 pKa = 6.39

Molecular weight: 21.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13951

0

13951

5779670

50

5727

414.3

46.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.361 ± 0.021

1.383 ± 0.008

5.662 ± 0.015

6.762 ± 0.027

4.069 ± 0.015

4.955 ± 0.02

2.572 ± 0.009

6.123 ± 0.016

6.549 ± 0.022

9.425 ± 0.022

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.481 ± 0.009

4.723 ± 0.014

4.689 ± 0.018

4.712 ± 0.019

4.86 ± 0.014

7.986 ± 0.022

6.089 ± 0.017

6.012 ± 0.015

1.179 ± 0.007

3.408 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski