Streptococcus phage Javan422

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6B401|A0A4D6B401_9CAUD Uncharacterized protein OS=Streptococcus phage Javan422 OX=2548158 GN=Javan422_0041 PE=4 SV=1
MM1 pKa = 6.25TTALFLLRR9 pKa = 11.84CVEE12 pKa = 4.13VGISISDD19 pKa = 4.07LDD21 pKa = 4.17LLSIGMVLDD30 pKa = 3.53IWTEE34 pKa = 3.82KK35 pKa = 11.2ANDD38 pKa = 3.45SVKK41 pKa = 9.62YY42 pKa = 10.68SRR44 pKa = 11.84VAGQAEE50 pKa = 4.08FDD52 pKa = 3.73KK53 pKa = 11.39FF54 pKa = 3.5

Molecular weight: 6.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6B394|A0A4D6B394_9CAUD DNA primase/helicase OS=Streptococcus phage Javan422 OX=2548158 GN=Javan422_0007 PE=4 SV=1
MM1 pKa = 7.37KK2 pKa = 9.98HH3 pKa = 4.79QLKK6 pKa = 10.54VSVSKK11 pKa = 9.83TPSLEE16 pKa = 4.19KK17 pKa = 10.61MMTCRR22 pKa = 11.84KK23 pKa = 9.86LSIRR27 pKa = 11.84EE28 pKa = 3.76KK29 pKa = 9.91ILRR32 pKa = 11.84FFFGKK37 pKa = 10.27KK38 pKa = 8.28QDD40 pKa = 3.93LVVLIPSDD48 pKa = 4.2RR49 pKa = 11.84ISEE52 pKa = 4.14VAISKK57 pKa = 10.28KK58 pKa = 10.73GEE60 pKa = 4.1SNEE63 pKa = 3.91

Molecular weight: 7.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

12335

38

935

274.1

31.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.967 ± 0.386

1.086 ± 0.168

5.553 ± 0.285

7.604 ± 0.419

3.859 ± 0.196

6.194 ± 0.37

1.881 ± 0.151

7.604 ± 0.316

8.626 ± 0.361

8.804 ± 0.305

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.4 ± 0.148

5.432 ± 0.272

2.886 ± 0.206

4.037 ± 0.225

4.062 ± 0.293

6.923 ± 0.328

5.918 ± 0.509

6.096 ± 0.254

1.338 ± 0.133

3.729 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski