Mycobacterium phage Hamulus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5XXW2|S5XXW2_9CAUD Uncharacterized protein OS=Mycobacterium phage Hamulus OX=1340824 GN=75 PE=4 SV=1
MM1 pKa = 6.72NTSTNRR7 pKa = 11.84EE8 pKa = 4.05YY9 pKa = 10.46PSYY12 pKa = 11.1AGDD15 pKa = 3.75LALTLDD21 pKa = 4.0TSGDD25 pKa = 3.51TAKK28 pKa = 9.3ITVAEE33 pKa = 4.12NDD35 pKa = 3.29NAVTVDD41 pKa = 4.45LDD43 pKa = 3.74AEE45 pKa = 4.47TARR48 pKa = 11.84EE49 pKa = 3.77LGTRR53 pKa = 11.84VIAYY57 pKa = 9.16NGSPSTWTIEE67 pKa = 3.81PMHH70 pKa = 6.62FGDD73 pKa = 4.99VEE75 pKa = 4.34LTVEE79 pKa = 4.58DD80 pKa = 4.17GQIILALPDD89 pKa = 3.51VTPFDD94 pKa = 5.34LGEE97 pKa = 4.18CDD99 pKa = 3.43IDD101 pKa = 4.22PFVHH105 pKa = 7.68DD106 pKa = 4.54FGQRR110 pKa = 11.84LIVWAGVDD118 pKa = 3.73LAVTADD124 pKa = 3.25AA125 pKa = 5.58

Molecular weight: 13.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5YA20|S5YA20_9CAUD DnaQ-like protein OS=Mycobacterium phage Hamulus OX=1340824 GN=36 PE=4 SV=1
MM1 pKa = 7.46TGPGARR7 pKa = 11.84QRR9 pKa = 11.84IPAPNPITGLSRR21 pKa = 11.84EE22 pKa = 4.16GLAVDD27 pKa = 4.22ATQEE31 pKa = 3.52QWRR34 pKa = 11.84PVVGYY39 pKa = 9.66EE40 pKa = 4.2GMYY43 pKa = 10.11EE44 pKa = 4.0VSDD47 pKa = 3.91LGRR50 pKa = 11.84VRR52 pKa = 11.84SVDD55 pKa = 2.96RR56 pKa = 11.84CVVTKK61 pKa = 10.55SGPRR65 pKa = 11.84TYY67 pKa = 10.39RR68 pKa = 11.84GRR70 pKa = 11.84LLHH73 pKa = 5.53QHH75 pKa = 6.62PDD77 pKa = 2.75GRR79 pKa = 11.84GYY81 pKa = 11.11LRR83 pKa = 11.84ASLSRR88 pKa = 11.84VGDD91 pKa = 3.61KK92 pKa = 10.65PRR94 pKa = 11.84MFKK97 pKa = 9.06VHH99 pKa = 6.88RR100 pKa = 11.84LVLEE104 pKa = 4.17AFVGPRR110 pKa = 11.84PGNLSGCHH118 pKa = 5.25NNGINTDD125 pKa = 3.3NRR127 pKa = 11.84LEE129 pKa = 3.91NLRR132 pKa = 11.84WDD134 pKa = 3.55SHH136 pKa = 4.99TEE138 pKa = 3.56NMLDD142 pKa = 3.6VVRR145 pKa = 11.84HH146 pKa = 5.38GRR148 pKa = 11.84HH149 pKa = 5.83HH150 pKa = 5.07YY151 pKa = 10.47AKK153 pKa = 10.07RR154 pKa = 11.84DD155 pKa = 3.55RR156 pKa = 11.84CPKK159 pKa = 9.06GHH161 pKa = 6.52VFNEE165 pKa = 4.08PNTLISPRR173 pKa = 11.84GARR176 pKa = 11.84VCRR179 pKa = 11.84TCQKK183 pKa = 10.44QYY185 pKa = 10.88NDD187 pKa = 3.98RR188 pKa = 11.84YY189 pKa = 9.46YY190 pKa = 10.02QTACDD195 pKa = 3.81EE196 pKa = 3.94KK197 pKa = 10.6RR198 pKa = 11.84ARR200 pKa = 11.84GWIPKK205 pKa = 9.63RR206 pKa = 11.84EE207 pKa = 3.94RR208 pKa = 11.84TKK210 pKa = 10.93CPLGHH215 pKa = 6.7EE216 pKa = 3.92YY217 pKa = 10.61DD218 pKa = 3.52YY219 pKa = 11.34FYY221 pKa = 11.65VNKK224 pKa = 10.32RR225 pKa = 11.84NGKK228 pKa = 6.05VTRR231 pKa = 11.84HH232 pKa = 6.06CKK234 pKa = 7.53TCRR237 pKa = 11.84AQNHH241 pKa = 5.53RR242 pKa = 11.84NFKK245 pKa = 9.89KK246 pKa = 10.08RR247 pKa = 11.84RR248 pKa = 11.84AGASCVV254 pKa = 3.28

Molecular weight: 29.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

105

0

105

18231

27

1159

173.6

18.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.361 ± 0.456

1.278 ± 0.171

6.944 ± 0.22

5.688 ± 0.298

3.006 ± 0.199

9.007 ± 0.632

2.227 ± 0.194

4.322 ± 0.194

3.368 ± 0.227

7.378 ± 0.21

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.298 ± 0.132

3.445 ± 0.167

6.209 ± 0.204

3.39 ± 0.154

6.9 ± 0.4

5.716 ± 0.269

6.648 ± 0.251

7.065 ± 0.308

2.304 ± 0.164

2.446 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski