Criblamydia sequanensis CRIB-18

Taxonomy: cellular organisms; Bacteria; PVC group; Chlamydiae; Chlamydiia; Parachlamydiales; Criblamydiaceae; Criblamydia; Criblamydia sequanensis

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2418 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A090D0Q2|A0A090D0Q2_9BACT Putative secreted protein OS=Criblamydia sequanensis CRIB-18 OX=1437425 GN=CSEC_0314 PE=4 SV=1
MM1 pKa = 7.71KK2 pKa = 10.68LNILPLEE9 pKa = 4.0EE10 pKa = 5.59RR11 pKa = 11.84IVLDD15 pKa = 3.3AAAAVVIAAPPTATDD30 pKa = 3.24SGDD33 pKa = 3.57AATHH37 pKa = 6.9DD38 pKa = 4.14SSQATDD44 pKa = 3.21SSQEE48 pKa = 3.85SGPAQEE54 pKa = 4.79SGVNVLVISSAVQDD68 pKa = 3.9PQSLQNAAKK77 pKa = 10.44DD78 pKa = 3.73GVKK81 pKa = 9.57TVFYY85 pKa = 10.22DD86 pKa = 3.68ANSTSLTEE94 pKa = 4.31LSQKK98 pKa = 9.46IANALNGQKK107 pKa = 10.33ADD109 pKa = 4.02SIAFAAEE116 pKa = 4.06GNDD119 pKa = 3.27NVLMLNTDD127 pKa = 2.87IMVSDD132 pKa = 5.21NIQSNIQNHH141 pKa = 7.06DD142 pKa = 2.79ISGFWKK148 pKa = 9.62TIGGLLNNGGRR159 pKa = 11.84IDD161 pKa = 5.72LLACDD166 pKa = 3.91VAGDD170 pKa = 3.79SGGQSLVAQIDD181 pKa = 3.63QLIDD185 pKa = 3.38RR186 pKa = 11.84PQDD189 pKa = 3.21GFFATVAASTDD200 pKa = 3.59LTGNAAGGDD209 pKa = 3.4WLLEE213 pKa = 4.07VGNIDD218 pKa = 4.1AKK220 pKa = 10.75SIYY223 pKa = 10.6FNDD226 pKa = 5.03SINNWNHH233 pKa = 4.62NMATAIEE240 pKa = 4.25YY241 pKa = 10.94GLIAALIGALLQPRR255 pKa = 11.84EE256 pKa = 4.17TLEE259 pKa = 4.11DD260 pKa = 3.34TSLSFSYY267 pKa = 10.65ASSGTVFANPDD278 pKa = 3.26FSFTLLTNSINGNTTVTEE296 pKa = 4.66DD297 pKa = 3.63GNVTYY302 pKa = 9.98TPNADD307 pKa = 3.52YY308 pKa = 11.2NNPGFTLGGTINDD321 pKa = 3.7GVSFDD326 pKa = 3.56SATIRR331 pKa = 11.84IVDD334 pKa = 4.24TNPDD338 pKa = 3.52DD339 pKa = 4.55PNSVFAINLPLYY351 pKa = 10.49LRR353 pKa = 11.84VVSVNDD359 pKa = 3.61APIAQNDD366 pKa = 4.05SFSIGQGNEE375 pKa = 3.49LTGNVALNDD384 pKa = 3.58SDD386 pKa = 4.16FQNGAPSEE394 pKa = 4.38NNTPLTVQLVSGPAHH409 pKa = 6.16AASFTLNADD418 pKa = 3.3GTFSYY423 pKa = 10.62TSIGSYY429 pKa = 10.24SGADD433 pKa = 2.95SFTYY437 pKa = 10.5SITDD441 pKa = 3.29SLGGVSEE448 pKa = 4.72ANVALSVISNAPPAVSDD465 pKa = 3.5NYY467 pKa = 10.29IINEE471 pKa = 3.95DD472 pKa = 3.39TTLNGNVTDD481 pKa = 4.34NDD483 pKa = 4.2TFNLGANQTIQLVSGPLVAANFALNSDD510 pKa = 4.01GSFSYY515 pKa = 10.59TPLANLVGVDD525 pKa = 3.28TFSYY529 pKa = 10.28VIKK532 pKa = 8.94TVSGAGITFSNTATVNIAVLPVNDD556 pKa = 4.17NPVAINDD563 pKa = 3.78NYY565 pKa = 10.94NVNEE569 pKa = 4.79DD570 pKa = 3.17GSLAANVLNNDD581 pKa = 2.98TDD583 pKa = 3.68IEE585 pKa = 4.37NNRR588 pKa = 11.84PFSAQLVAGPAHH600 pKa = 6.62AGSFTLNADD609 pKa = 3.43GTFSYY614 pKa = 10.69IPTADD619 pKa = 3.63YY620 pKa = 11.14NGSDD624 pKa = 3.11SFTYY628 pKa = 9.45VTVDD632 pKa = 3.05SLGAISNLATVSIAVASINDD652 pKa = 3.53APVAQDD658 pKa = 2.72VSYY661 pKa = 9.18NTNEE665 pKa = 4.02DD666 pKa = 3.75TPLTGILGVGSDD678 pKa = 4.2SQNGAPNEE686 pKa = 4.49SNTPLTAQLIGGPAHH701 pKa = 7.27ASAFTLNADD710 pKa = 3.67GSFSYY715 pKa = 10.85NPQADD720 pKa = 3.96FNGTDD725 pKa = 3.01SFTYY729 pKa = 10.27AIVDD733 pKa = 3.62SLGAISNTATVSITIDD749 pKa = 3.59SVNDD753 pKa = 3.36APIAQNDD760 pKa = 3.69AFQGNEE766 pKa = 3.96DD767 pKa = 3.47GSINGSVLLNDD778 pKa = 4.47SDD780 pKa = 4.27NQNGAPNEE788 pKa = 4.2NNSPLSAQLVTGPAHH803 pKa = 6.72AGSFTLNADD812 pKa = 3.38GTFTYY817 pKa = 10.23IPSADD822 pKa = 3.73YY823 pKa = 11.04NGSDD827 pKa = 2.98SFTYY831 pKa = 9.37VTLDD835 pKa = 3.29SLGGISNLATVSITINSVNDD855 pKa = 3.38APIAQNDD862 pKa = 4.16SYY864 pKa = 11.51QVNEE868 pKa = 4.2DD869 pKa = 3.18NGLNGNVALNDD880 pKa = 3.64SDD882 pKa = 4.23SQNGAPSEE890 pKa = 4.28NNGPLSAQLVTGPAHH905 pKa = 6.72AGSFTLNADD914 pKa = 3.43GTFSYY919 pKa = 10.62IPAANYY925 pKa = 10.17NGADD929 pKa = 3.15SFTYY933 pKa = 10.57VIVDD937 pKa = 3.55SLGATSNIATVAITINSVNDD957 pKa = 3.5APIVQNDD964 pKa = 3.61SFNIPGNLPYY974 pKa = 10.58SGNVLTNDD982 pKa = 3.68SDD984 pKa = 4.25NQGGAPNEE992 pKa = 4.0NNIPLSAQLVNGPAHH1007 pKa = 5.84ATSFILNADD1016 pKa = 3.64GTFSYY1021 pKa = 10.88QPANGFVGSDD1031 pKa = 2.88TFTYY1035 pKa = 10.4RR1036 pKa = 11.84VTDD1039 pKa = 3.64SLGGTSTIATVTITVAQAASANPILEE1065 pKa = 4.19LKK1067 pKa = 11.02GNINKK1072 pKa = 10.0NYY1074 pKa = 9.34IEE1076 pKa = 4.19NSGPVILSNNVKK1088 pKa = 8.65ITDD1091 pKa = 3.7SDD1093 pKa = 3.7SSNFDD1098 pKa = 3.08GGKK1101 pKa = 8.46LTVQIDD1107 pKa = 3.69QNGQSSDD1114 pKa = 3.06ILSIDD1119 pKa = 3.22NSTKK1123 pKa = 10.54VKK1125 pKa = 10.12TNANQDD1131 pKa = 2.6IYY1133 pKa = 11.73YY1134 pKa = 10.47NGLLVGHH1141 pKa = 5.5YY1142 pKa = 9.29TGGSGLASLEE1152 pKa = 3.74ITFNANATANVVSEE1166 pKa = 4.12VAEE1169 pKa = 4.42RR1170 pKa = 11.84IAYY1173 pKa = 8.97KK1174 pKa = 10.56NSSEE1178 pKa = 4.22NPSTADD1184 pKa = 3.33RR1185 pKa = 11.84SIRR1188 pKa = 11.84YY1189 pKa = 8.39QLSDD1193 pKa = 3.4GDD1195 pKa = 4.23SGISEE1200 pKa = 4.21AQYY1203 pKa = 10.56KK1204 pKa = 8.77IVRR1207 pKa = 11.84VFSVNDD1213 pKa = 3.54APDD1216 pKa = 3.06ISIADD1221 pKa = 3.44NTLTVNRR1228 pKa = 11.84GSITNIGALSGIQISDD1244 pKa = 3.19VDD1246 pKa = 3.69ASAGDD1251 pKa = 3.61RR1252 pKa = 11.84VRR1254 pKa = 11.84VVLKK1258 pKa = 10.84VLVGYY1263 pKa = 11.05LDD1265 pKa = 3.68FTDD1268 pKa = 5.09DD1269 pKa = 5.06LNDD1272 pKa = 3.36LTSIIFKK1279 pKa = 10.44RR1280 pKa = 11.84NPGDD1284 pKa = 3.49GLGSLDD1290 pKa = 3.84FTGTLADD1297 pKa = 3.4VNAALEE1303 pKa = 4.06RR1304 pKa = 11.84LTYY1307 pKa = 10.3RR1308 pKa = 11.84SRR1310 pKa = 11.84TNYY1313 pKa = 9.64IGLDD1317 pKa = 3.36ALTVGVSDD1325 pKa = 4.21MGSSGTFAIPRR1336 pKa = 11.84IDD1338 pKa = 4.99ADD1340 pKa = 3.53WLLLKK1345 pKa = 10.3IVV1347 pKa = 3.84

Molecular weight: 139.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A090D0X6|A0A090D0X6_9BACT Uncharacterized protein OS=Criblamydia sequanensis CRIB-18 OX=1437425 GN=CSEC_2205 PE=4 SV=1
MM1 pKa = 6.88LQEE4 pKa = 4.07YY5 pKa = 10.6VDD7 pKa = 3.23TGRR10 pKa = 11.84RR11 pKa = 11.84KK12 pKa = 7.4TAVASVRR19 pKa = 11.84LRR21 pKa = 11.84SGTGKK26 pKa = 10.33VDD28 pKa = 3.23INGKK32 pKa = 9.9AFEE35 pKa = 4.77EE36 pKa = 4.43YY37 pKa = 10.5FPLDD41 pKa = 3.62MQRR44 pKa = 11.84KK45 pKa = 8.27LALAPLGVVNVTGQFDD61 pKa = 3.87LLIRR65 pKa = 11.84VKK67 pKa = 10.86GGGVEE72 pKa = 4.39GQAEE76 pKa = 4.31AVRR79 pKa = 11.84LGLARR84 pKa = 11.84ALIQVNEE91 pKa = 4.06AFKK94 pKa = 11.25HH95 pKa = 4.43EE96 pKa = 4.37FKK98 pKa = 11.29VLGFLTRR105 pKa = 11.84DD106 pKa = 2.93SRR108 pKa = 11.84MKK110 pKa = 10.08EE111 pKa = 3.46RR112 pKa = 11.84KK113 pKa = 9.15KK114 pKa = 10.69YY115 pKa = 9.99GRR117 pKa = 11.84AGARR121 pKa = 11.84KK122 pKa = 9.15RR123 pKa = 11.84FQFSKK128 pKa = 10.78RR129 pKa = 3.42

Molecular weight: 14.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2418

0

2418

885973

29

4164

366.4

41.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.536 ± 0.045

1.044 ± 0.022

4.893 ± 0.034

7.716 ± 0.05

5.626 ± 0.044

5.873 ± 0.055

1.999 ± 0.02

7.294 ± 0.039

8.269 ± 0.055

11.439 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.932 ± 0.025

4.555 ± 0.039

4.202 ± 0.04

3.288 ± 0.03

4.084 ± 0.032

7.23 ± 0.046

4.655 ± 0.032

5.159 ± 0.04

1.013 ± 0.017

3.194 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski