Gillisia sp. Hel1_33_143

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Gillisia; unclassified Gillisia

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3141 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H1VGS7|A0A1H1VGS7_9FLAO Ammonium transporter OS=Gillisia sp. Hel1_33_143 OX=1336796 GN=SAMN04487764_3186 PE=3 SV=1
MM1 pKa = 7.64KK2 pKa = 10.4NFKK5 pKa = 10.43FLIAFAAMIILLFTYY20 pKa = 10.35CSKK23 pKa = 11.53DD24 pKa = 3.45DD25 pKa = 4.26VNDD28 pKa = 3.6QNSEE32 pKa = 4.33TNSEE36 pKa = 4.1LVSISFDD43 pKa = 3.5AVLKK47 pKa = 10.74DD48 pKa = 3.74FNTNRR53 pKa = 11.84SLTKK57 pKa = 10.19QNQLADD63 pKa = 3.46IPEE66 pKa = 4.54CLDD69 pKa = 4.09NIPSKK74 pKa = 11.14VRR76 pKa = 11.84VALKK80 pKa = 10.21DD81 pKa = 3.62SEE83 pKa = 4.56GAWVSMANGDD93 pKa = 3.42AGEE96 pKa = 4.52FIEE99 pKa = 5.61IPVIANDD106 pKa = 3.22EE107 pKa = 4.44GGWMTKK113 pKa = 9.66EE114 pKa = 4.11SADD117 pKa = 4.21LEE119 pKa = 4.63LPPGDD124 pKa = 3.5YY125 pKa = 9.9TLEE128 pKa = 4.02YY129 pKa = 10.26FAVLDD134 pKa = 3.68TDD136 pKa = 5.41NNVLWIAPRR145 pKa = 11.84EE146 pKa = 3.94NDD148 pKa = 4.22DD149 pKa = 3.6YY150 pKa = 12.04GPANFANFVSDD161 pKa = 4.69ALPITIDD168 pKa = 3.28LRR170 pKa = 11.84AGVKK174 pKa = 10.01KK175 pKa = 10.94YY176 pKa = 10.43IDD178 pKa = 3.99VEE180 pKa = 4.33VLCYY184 pKa = 9.61DD185 pKa = 3.22EE186 pKa = 6.3RR187 pKa = 11.84MADD190 pKa = 3.21EE191 pKa = 4.13YY192 pKa = 11.48GYY194 pKa = 11.4LFFDD198 pKa = 4.92FSQVDD203 pKa = 4.65AITLCLFGNYY213 pKa = 9.47CDD215 pKa = 3.57VTGRR219 pKa = 11.84HH220 pKa = 4.57YY221 pKa = 10.19PARR224 pKa = 11.84FSVEE228 pKa = 3.21AWIFSGDD235 pKa = 3.52LADD238 pKa = 4.74PKK240 pKa = 10.74LGEE243 pKa = 4.32PLTNGILTNEE253 pKa = 3.94TGIYY257 pKa = 10.17EE258 pKa = 4.16DD259 pKa = 4.47TEE261 pKa = 4.38DD262 pKa = 3.49AFARR266 pKa = 11.84PLCIALPNSSDD277 pKa = 3.04EE278 pKa = 3.81DD279 pKa = 3.84TYY281 pKa = 11.6YY282 pKa = 10.13IEE284 pKa = 4.68IKK286 pKa = 10.72LLGFEE291 pKa = 4.73GVYY294 pKa = 9.98TDD296 pKa = 3.93PEE298 pKa = 4.71VPVEE302 pKa = 3.99TLMITDD308 pKa = 4.13TEE310 pKa = 4.35VLDD313 pKa = 4.58LYY315 pKa = 11.07NGEE318 pKa = 4.49SNTYY322 pKa = 7.25YY323 pKa = 10.46HH324 pKa = 6.72FRR326 pKa = 11.84IGCEE330 pKa = 4.04GTEE333 pKa = 3.98EE334 pKa = 4.4CANDD338 pKa = 3.64MDD340 pKa = 5.6CDD342 pKa = 4.98GIADD346 pKa = 4.53DD347 pKa = 5.83VDD349 pKa = 3.81NCPMKK354 pKa = 11.05ANADD358 pKa = 3.65QTDD361 pKa = 3.46TDD363 pKa = 3.8EE364 pKa = 6.14DD365 pKa = 4.62GVGDD369 pKa = 3.98VCDD372 pKa = 3.54NCVNIANADD381 pKa = 3.79QVDD384 pKa = 3.66ADD386 pKa = 3.6QDD388 pKa = 4.44GIGDD392 pKa = 3.76ACEE395 pKa = 4.84DD396 pKa = 4.64LSTDD400 pKa = 3.87DD401 pKa = 6.01DD402 pKa = 5.26GDD404 pKa = 4.05GVPNDD409 pKa = 3.37IDD411 pKa = 3.91EE412 pKa = 4.99CPGTPSGTPVDD423 pKa = 3.47IKK425 pKa = 10.78GCEE428 pKa = 4.25SIQVPGRR435 pKa = 11.84DD436 pKa = 2.63VVVFNDD442 pKa = 4.05ANMFDD447 pKa = 4.49DD448 pKa = 5.67NAMEE452 pKa = 4.84DD453 pKa = 3.64PDD455 pKa = 3.9NIRR458 pKa = 11.84FVEE461 pKa = 4.07NLVTYY466 pKa = 7.59TTTGSRR472 pKa = 11.84NAGDD476 pKa = 3.81VVWIDD481 pKa = 3.38RR482 pKa = 11.84GRR484 pKa = 11.84SAACYY489 pKa = 10.69SNGEE493 pKa = 4.2CDD495 pKa = 3.31EE496 pKa = 5.26DD497 pKa = 3.69GWSKK501 pKa = 10.4MEE503 pKa = 3.83EE504 pKa = 4.39TITNTGLTVTSVFSNAGSLTNIPADD529 pKa = 3.44VKK531 pKa = 11.11VIFLVMPTLQYY542 pKa = 10.31TVAEE546 pKa = 4.19INAFKK551 pKa = 10.97AFAAEE556 pKa = 4.12GGRR559 pKa = 11.84IIFVGEE565 pKa = 3.63HH566 pKa = 5.09SSFYY570 pKa = 10.94FFIDD574 pKa = 3.31VQNQFLLNMGAVLTNTGGLVDD595 pKa = 4.5CGYY598 pKa = 9.08TVIPQSSNRR607 pKa = 11.84VHH609 pKa = 7.31PIMAGITDD617 pKa = 4.09LTIACASVIEE627 pKa = 4.4IGVDD631 pKa = 3.65DD632 pKa = 4.6FALFYY637 pKa = 9.97DD638 pKa = 4.01TTNTKK643 pKa = 10.0VLAGVAKK650 pKa = 10.13IDD652 pKa = 3.5TTPISEE658 pKa = 4.76LKK660 pKa = 10.51ASSIRR665 pKa = 11.84FDD667 pKa = 3.42NPVRR671 pKa = 11.84NRR673 pKa = 11.84NSKK676 pKa = 8.56STSSGIQPP684 pKa = 3.73

Molecular weight: 74.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H1SQJ5|A0A1H1SQJ5_9FLAO Uncharacterized protein OS=Gillisia sp. Hel1_33_143 OX=1336796 GN=SAMN04487764_2365 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.15RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84KK13 pKa = 8.29NKK15 pKa = 9.34HH16 pKa = 4.03GFRR19 pKa = 11.84EE20 pKa = 4.05RR21 pKa = 11.84MASVNGRR28 pKa = 11.84KK29 pKa = 9.21VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.09GRR39 pKa = 11.84KK40 pKa = 7.97KK41 pKa = 10.73LSVSSEE47 pKa = 3.76NRR49 pKa = 11.84HH50 pKa = 4.47KK51 pKa = 10.5HH52 pKa = 4.49

Molecular weight: 6.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3141

0

3141

1034711

34

3446

329.4

37.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.393 ± 0.036

0.687 ± 0.014

5.625 ± 0.04

6.935 ± 0.044

5.178 ± 0.038

6.243 ± 0.046

1.694 ± 0.023

8.155 ± 0.047

7.858 ± 0.056

9.565 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.287 ± 0.025

6.074 ± 0.048

3.304 ± 0.021

3.372 ± 0.024

3.503 ± 0.029

6.696 ± 0.035

5.419 ± 0.053

6.023 ± 0.038

0.99 ± 0.016

3.999 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski