Llama faeces associated circular DNA virus-1
Average proteome isoelectric point is 7.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A160HWJ8|A0A160HWJ8_9VIRU Putative capsid protein OS=Llama faeces associated circular DNA virus-1 OX=1843771 PE=4 SV=1
MM1 pKa = 7.86 PSNPQFFHH9 pKa = 7.65 FCFTLNNYY17 pKa = 8.62 VEE19 pKa = 4.5 EE20 pKa = 4.22 VLDD23 pKa = 4.06 DD24 pKa = 3.97 VEE26 pKa = 4.93 EE27 pKa = 4.32 EE28 pKa = 4.1 DD29 pKa = 4.27 VPRR32 pKa = 11.84 ISAFCEE38 pKa = 3.89 EE39 pKa = 4.43 EE40 pKa = 3.81 GKK42 pKa = 10.62 YY43 pKa = 9.68 WIIGRR48 pKa = 11.84 EE49 pKa = 4.18 VGEE52 pKa = 4.35 SGTPHH57 pKa = 5.68 LQGYY61 pKa = 9.13 VSLRR65 pKa = 11.84 KK66 pKa = 9.36 RR67 pKa = 11.84 RR68 pKa = 11.84 TFVYY72 pKa = 10.61 VRR74 pKa = 11.84 DD75 pKa = 3.82 KK76 pKa = 11.33 LSNRR80 pKa = 11.84 CHH82 pKa = 6.55 IEE84 pKa = 3.7 SSRR87 pKa = 11.84 GTARR91 pKa = 11.84 QNRR94 pKa = 11.84 EE95 pKa = 3.81 YY96 pKa = 10.52 CSKK99 pKa = 10.71 GGNFIEE105 pKa = 4.94 GGSINEE111 pKa = 3.69 GRR113 pKa = 11.84 VRR115 pKa = 11.84 QDD117 pKa = 2.34 RR118 pKa = 11.84 DD119 pKa = 2.82 EE120 pKa = 4.26 AARR123 pKa = 11.84 SFMAAVRR130 pKa = 11.84 RR131 pKa = 11.84 GDD133 pKa = 3.27 QGLVEE138 pKa = 4.45 FADD141 pKa = 4.29 AEE143 pKa = 4.05 PHH145 pKa = 4.11 TWIRR149 pKa = 11.84 HH150 pKa = 4.19 GSNMLRR156 pKa = 11.84 NALSILPPIEE166 pKa = 4.3 RR167 pKa = 11.84 PSISVRR173 pKa = 11.84 WIWGSPGVGKK183 pKa = 10.57 SRR185 pKa = 11.84 IAHH188 pKa = 5.28 EE189 pKa = 4.58 TLPDD193 pKa = 3.72 AYY195 pKa = 11.22 VKK197 pKa = 10.61 DD198 pKa = 4.61 PRR200 pKa = 11.84 TKK202 pKa = 8.07 WWNGYY207 pKa = 8.98 LCQEE211 pKa = 4.71 DD212 pKa = 5.12 VIIDD216 pKa = 3.66 DD217 pKa = 4.93 FGPNGIDD224 pKa = 3.32 INHH227 pKa = 7.46 LLRR230 pKa = 11.84 WFDD233 pKa = 3.45 RR234 pKa = 11.84 YY235 pKa = 10.77 KK236 pKa = 11.0 CLVEE240 pKa = 4.28 NKK242 pKa = 9.85 GGMVALHH249 pKa = 5.86 ATNFIVTSNFHH260 pKa = 6.34 PRR262 pKa = 11.84 DD263 pKa = 3.08 IFKK266 pKa = 10.81 FGDD269 pKa = 3.95 EE270 pKa = 4.81 INPQLPALEE279 pKa = 3.79 RR280 pKa = 11.84 RR281 pKa = 11.84 IVIEE285 pKa = 3.93 EE286 pKa = 3.96 MLL288 pKa = 4.23
Molecular weight: 33.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.782
IPC2_protein 5.728
IPC_protein 5.766
Toseland 6.135
ProMoST 6.097
Dawson 6.008
Bjellqvist 6.02
Wikipedia 6.02
Rodwell 5.995
Grimsley 6.262
Solomon 6.008
Lehninger 6.008
Nozaki 6.275
DTASelect 6.453
Thurlkill 6.465
EMBOSS 6.427
Sillero 6.376
Patrickios 4.304
IPC_peptide 6.033
IPC2_peptide 6.376
IPC2.peptide.svr19 6.336
Protein with the highest isoelectric point:
>tr|A0A160HWJ8|A0A160HWJ8_9VIRU Putative capsid protein OS=Llama faeces associated circular DNA virus-1 OX=1843771 PE=4 SV=1
MM1 pKa = 7.29 AFKK4 pKa = 10.49 RR5 pKa = 11.84 KK6 pKa = 9.11 RR7 pKa = 11.84 VYY9 pKa = 10.71 APRR12 pKa = 11.84 ANAFKK17 pKa = 10.42 KK18 pKa = 10.13 RR19 pKa = 11.84 KK20 pKa = 8.62 SAMRR24 pKa = 11.84 RR25 pKa = 11.84 KK26 pKa = 9.58 RR27 pKa = 11.84 RR28 pKa = 11.84 NSKK31 pKa = 9.82 GGPRR35 pKa = 11.84 VVGYY39 pKa = 8.88 TSLNQKK45 pKa = 9.86 DD46 pKa = 3.42 HH47 pKa = 6.27 TFGFRR52 pKa = 11.84 ARR54 pKa = 11.84 KK55 pKa = 6.74 TSRR58 pKa = 11.84 RR59 pKa = 11.84 AFKK62 pKa = 10.46 KK63 pKa = 10.1 HH64 pKa = 5.74 LWDD67 pKa = 3.29 STLYY71 pKa = 9.69 ANHH74 pKa = 6.49 WRR76 pKa = 11.84 SFSSSQQNVTTPAGTLDD93 pKa = 3.46 TALFAINLTSPIGNTSPFWTAAGGLVPTDD122 pKa = 3.34 IGVTPPKK129 pKa = 10.25 FVGDD133 pKa = 3.21 ITLRR137 pKa = 11.84 GGVWEE142 pKa = 4.66 CIIYY146 pKa = 9.78 NQSTTQDD153 pKa = 2.73 IRR155 pKa = 11.84 FKK157 pKa = 9.68 SWLVRR162 pKa = 11.84 TVDD165 pKa = 3.73 TPNFTPFPATSPISWEE181 pKa = 3.71 PSIAADD187 pKa = 3.94 FVQYY191 pKa = 10.03 IGKK194 pKa = 8.49 PFQSRR199 pKa = 11.84 EE200 pKa = 3.87 VVIEE204 pKa = 3.99 SGNSYY209 pKa = 7.49 TLKK212 pKa = 10.2 GRR214 pKa = 11.84 YY215 pKa = 8.48 RR216 pKa = 11.84 VEE218 pKa = 5.29 KK219 pKa = 10.04 IDD221 pKa = 3.72 QKK223 pKa = 10.04 VQAVAGRR230 pKa = 11.84 QLFLFGLIHH239 pKa = 6.34 NVGSAGAVAQTMNIVTSQNLSFAADD264 pKa = 3.39 AVGTTT269 pKa = 3.9
Molecular weight: 29.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.315
IPC2_protein 9.911
IPC_protein 10.716
Toseland 10.921
ProMoST 10.613
Dawson 10.994
Bjellqvist 10.701
Wikipedia 11.213
Rodwell 11.286
Grimsley 11.038
Solomon 11.125
Lehninger 11.096
Nozaki 10.891
DTASelect 10.701
Thurlkill 10.906
EMBOSS 11.33
Sillero 10.935
Patrickios 10.994
IPC_peptide 11.14
IPC2_peptide 9.531
IPC2.peptide.svr19 8.479
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
557
269
288
278.5
31.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.822 ± 1.333
1.257 ± 0.562
4.847 ± 0.717
5.386 ± 2.239
5.745 ± 0.365
7.72 ± 0.181
2.334 ± 0.538
6.284 ± 0.685
4.847 ± 0.935
5.745 ± 0.343
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.436 ± 0.204
5.206 ± 0.237
5.206 ± 0.001
3.411 ± 0.666
8.618 ± 0.515
7.002 ± 0.747
6.104 ± 2.024
7.002 ± 0.275
2.334 ± 0.066
2.693 ± 0.058
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here