Hubei sobemo-like virus 49

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEH2|A0A1L3KEH2_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 49 OX=1923237 PE=4 SV=1
MM1 pKa = 7.3TSSPGYY7 pKa = 9.38PYY9 pKa = 9.94MRR11 pKa = 11.84EE12 pKa = 3.97ATTNALWLKK21 pKa = 9.37WDD23 pKa = 3.76GVNVDD28 pKa = 3.43SMQKK32 pKa = 9.2QRR34 pKa = 11.84LWHH37 pKa = 6.53DD38 pKa = 3.63VQLVLSDD45 pKa = 3.63EE46 pKa = 4.47FEE48 pKa = 4.7GYY50 pKa = 10.67LRR52 pKa = 11.84VFVKK56 pKa = 10.06QEE58 pKa = 3.47PHH60 pKa = 6.12KK61 pKa = 9.95QRR63 pKa = 11.84KK64 pKa = 7.8MLDD67 pKa = 3.23KK68 pKa = 10.27RR69 pKa = 11.84WRR71 pKa = 11.84LIVASSLPVQVAWHH85 pKa = 5.3MLFYY89 pKa = 10.64QLNDD93 pKa = 3.6LEE95 pKa = 5.36IDD97 pKa = 3.44NSYY100 pKa = 10.43EE101 pKa = 3.84IPSQQGILLVKK112 pKa = 10.29GGWRR116 pKa = 11.84QYY118 pKa = 8.66YY119 pKa = 9.69NSWVEE124 pKa = 3.85RR125 pKa = 11.84GLVCGLDD132 pKa = 3.27KK133 pKa = 11.29SAWDD137 pKa = 3.15WTAPKK142 pKa = 9.41WAIDD146 pKa = 3.29MDD148 pKa = 4.09LEE150 pKa = 4.1LRR152 pKa = 11.84RR153 pKa = 11.84RR154 pKa = 11.84LGRR157 pKa = 11.84GDD159 pKa = 5.96KK160 pKa = 9.55MDD162 pKa = 3.23EE163 pKa = 3.47WHH165 pKa = 7.4RR166 pKa = 11.84IAKK169 pKa = 9.51SLYY172 pKa = 8.33RR173 pKa = 11.84RR174 pKa = 11.84MFEE177 pKa = 4.02DD178 pKa = 3.74PKK180 pKa = 11.14LILSDD185 pKa = 3.34GTVYY189 pKa = 9.3QQKK192 pKa = 9.44VPGIMKK198 pKa = 9.79SGCVNTISTNSHH210 pKa = 5.23CQVFVHH216 pKa = 6.51LAVCIEE222 pKa = 4.44NNLPMYY228 pKa = 8.36PLPVCCGDD236 pKa = 3.71DD237 pKa = 3.29TLQAVVHH244 pKa = 5.97VNDD247 pKa = 3.92LEE249 pKa = 4.07AYY251 pKa = 8.95RR252 pKa = 11.84RR253 pKa = 11.84FGVVVKK259 pKa = 10.47SASDD263 pKa = 3.48NIEE266 pKa = 4.12FVGHH270 pKa = 6.13EE271 pKa = 4.04FDD273 pKa = 5.04ARR275 pKa = 11.84GPQPMYY281 pKa = 10.39ILKK284 pKa = 9.89HH285 pKa = 5.31LKK287 pKa = 9.55KK288 pKa = 10.38VQYY291 pKa = 7.6TTDD294 pKa = 4.09EE295 pKa = 4.21ILSQYY300 pKa = 9.32LDD302 pKa = 3.25SMARR306 pKa = 11.84MYY308 pKa = 9.2THH310 pKa = 6.73SDD312 pKa = 4.06YY313 pKa = 11.45YY314 pKa = 10.5VVWEE318 pKa = 4.36TLAEE322 pKa = 4.01EE323 pKa = 4.46LGIDD327 pKa = 3.58LPMSRR332 pKa = 11.84EE333 pKa = 4.29SYY335 pKa = 9.19TYY337 pKa = 9.7WYY339 pKa = 10.23DD340 pKa = 3.65YY341 pKa = 11.57DD342 pKa = 3.38WW343 pKa = 5.71

Molecular weight: 40.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEH2|A0A1L3KEH2_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 49 OX=1923237 PE=4 SV=1
MM1 pKa = 7.61ACFSDD6 pKa = 3.65MLIGFDD12 pKa = 3.47IEE14 pKa = 4.22PVEE17 pKa = 4.09RR18 pKa = 11.84RR19 pKa = 11.84SLSGVVMDD27 pKa = 4.54GTHH30 pKa = 6.46FVYY33 pKa = 10.65EE34 pKa = 4.23SAKK37 pKa = 11.03DD38 pKa = 3.62LVTTVAQTRR47 pKa = 11.84TKK49 pKa = 11.11GEE51 pKa = 4.0IVLGTMVFGGLVYY64 pKa = 10.35KK65 pKa = 10.39CYY67 pKa = 10.1EE68 pKa = 3.86YY69 pKa = 11.15SVPTKK74 pKa = 10.27ILRR77 pKa = 11.84CVSSVVPGYY86 pKa = 10.1RR87 pKa = 11.84WVKK90 pKa = 10.55ARR92 pKa = 11.84LGKK95 pKa = 10.56YY96 pKa = 9.69DD97 pKa = 3.66VVSAAPRR104 pKa = 11.84RR105 pKa = 11.84MDD107 pKa = 3.02SVLEE111 pKa = 3.95SRR113 pKa = 11.84RR114 pKa = 11.84TGSDD118 pKa = 3.58EE119 pKa = 4.31TPMTPPRR126 pKa = 11.84HH127 pKa = 4.96QAVVAIRR134 pKa = 11.84DD135 pKa = 3.62GDD137 pKa = 3.59IMRR140 pKa = 11.84VLGCAVRR147 pKa = 11.84FNNTLVGPDD156 pKa = 3.55HH157 pKa = 6.77VLGGTPDD164 pKa = 3.08VEE166 pKa = 4.53KK167 pKa = 10.4FVKK170 pKa = 10.75GSQSWVALASRR181 pKa = 11.84EE182 pKa = 4.17RR183 pKa = 11.84IPLDD187 pKa = 3.23TDD189 pKa = 3.93LVAIEE194 pKa = 4.68MSDD197 pKa = 3.73KK198 pKa = 11.05DD199 pKa = 3.71FASIGISACKK209 pKa = 8.43VAPMGNQTEE218 pKa = 4.63FAQIVGPDD226 pKa = 3.16AKK228 pKa = 10.44GTVGQLKK235 pKa = 10.14HH236 pKa = 6.82DD237 pKa = 5.54AICFGRR243 pKa = 11.84LVYY246 pKa = 10.34TGTTIQGYY254 pKa = 9.69SGAAYY259 pKa = 9.56TMGPKK264 pKa = 10.39VMGIHH269 pKa = 5.61QMGGAVNGGYY279 pKa = 9.45SAEE282 pKa = 4.24YY283 pKa = 10.13INNLLLVHH291 pKa = 6.61FKK293 pKa = 10.36QRR295 pKa = 11.84YY296 pKa = 5.82EE297 pKa = 4.39SSPEE301 pKa = 3.69WLLSQYY307 pKa = 10.38EE308 pKa = 4.24AGRR311 pKa = 11.84TMSWKK316 pKa = 9.19RR317 pKa = 11.84TGDD320 pKa = 3.26PSTYY324 pKa = 8.14QLKK327 pKa = 10.64VGGRR331 pKa = 11.84YY332 pKa = 9.86ALVDD336 pKa = 4.16RR337 pKa = 11.84DD338 pKa = 4.38DD339 pKa = 3.51MDD341 pKa = 4.14AAFGEE346 pKa = 4.38HH347 pKa = 5.65WRR349 pKa = 11.84DD350 pKa = 3.56SEE352 pKa = 4.63VIQNKK357 pKa = 9.26RR358 pKa = 11.84DD359 pKa = 3.44VRR361 pKa = 11.84HH362 pKa = 6.49RR363 pKa = 11.84DD364 pKa = 3.49YY365 pKa = 11.41EE366 pKa = 4.49STGAVANFLSNSAVSAPSSSGEE388 pKa = 4.01ANGSRR393 pKa = 11.84NLGALSVVDD402 pKa = 4.6DD403 pKa = 4.19SQGLEE408 pKa = 3.58MSGPQKK414 pKa = 11.03LMAEE418 pKa = 4.24YY419 pKa = 10.65AKK421 pKa = 10.77LSNAQRR427 pKa = 11.84KK428 pKa = 8.38RR429 pKa = 11.84FRR431 pKa = 11.84QLSNLSPRR439 pKa = 11.84PIEE442 pKa = 4.06ATAGQARR449 pKa = 11.84QTNSQSS455 pKa = 2.99

Molecular weight: 49.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

798

343

455

399.0

45.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.642 ± 1.572

1.504 ± 0.17

6.642 ± 0.65

5.138 ± 0.278

2.632 ± 0.207

7.644 ± 1.661

2.256 ± 0.457

3.885 ± 0.136

4.762 ± 0.337

8.145 ± 1.225

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.759 ± 0.223

3.133 ± 0.051

4.135 ± 0.037

4.511 ± 0.308

6.516 ± 0.475

7.895 ± 1.026

4.762 ± 0.673

8.897 ± 0.307

2.381 ± 1.178

4.762 ± 0.943
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski