Escherichia phage NJ01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Kuravirus; Escherichia virus NJ01

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 109 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4ICF5|K4ICF5_9CAUD Uncharacterized protein OS=Escherichia phage NJ01 OX=1237159 GN=NJ01_047 PE=4 SV=1
MM1 pKa = 7.56ALFGGGNSKK10 pKa = 7.64TTSQMIRR17 pKa = 11.84PEE19 pKa = 4.35YY20 pKa = 7.84IQQYY24 pKa = 10.06IDD26 pKa = 3.97QLNQQIGNTSSGDD39 pKa = 3.33YY40 pKa = 10.43VYY42 pKa = 10.96RR43 pKa = 11.84DD44 pKa = 2.98NVGFNNNQTEE54 pKa = 4.16ALNNLAQSGALGTLSQQYY72 pKa = 9.36MDD74 pKa = 4.46AAGQGLGYY82 pKa = 10.18LDD84 pKa = 3.91NAYY87 pKa = 9.77KK88 pKa = 10.68GYY90 pKa = 10.55QSLSGQGGVTGEE102 pKa = 4.27QIGALAGQLYY112 pKa = 10.8DD113 pKa = 4.67DD114 pKa = 5.52DD115 pKa = 5.07AVQAAITANNEE126 pKa = 3.74QVQQNLARR134 pKa = 11.84NALPQLAQQYY144 pKa = 10.54AGQQGSGARR153 pKa = 11.84MAKK156 pKa = 10.07SFAQGDD162 pKa = 4.43ALNQMQGQATDD173 pKa = 3.61ITNSAYY179 pKa = 10.88NSAISQAEE187 pKa = 4.25SILSGNRR194 pKa = 11.84QNQAAALSGLSTIGSNLSNLGQQGANLSQQQMMNQWNAGLQQQQQQQNEE243 pKa = 4.18YY244 pKa = 11.28DD245 pKa = 3.32NAYY248 pKa = 10.94QNAQNAANWGWQDD261 pKa = 3.36INNQLGAAGVLNGALGQTTTTKK283 pKa = 10.35TSGGGGGFLGGAMSGAAAGSSFGPWGALAGGVIGGMASSS322 pKa = 3.71

Molecular weight: 33.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4I3T7|K4I3T7_9CAUD Uncharacterized protein OS=Escherichia phage NJ01 OX=1237159 GN=NJ01_094 PE=4 SV=1
MM1 pKa = 7.66RR2 pKa = 11.84PITMRR7 pKa = 11.84QFWLAKK13 pKa = 9.85IKK15 pKa = 9.25WASGGMTMTALPTRR29 pKa = 11.84QQARR33 pKa = 11.84DD34 pKa = 3.7FKK36 pKa = 10.51KK37 pKa = 9.3TMLTGDD43 pKa = 3.86VVNSISIHH51 pKa = 5.12KK52 pKa = 9.92AKK54 pKa = 9.84EE55 pKa = 3.85YY56 pKa = 10.59SSGRR60 pKa = 11.84VYY62 pKa = 10.76ISGKK66 pKa = 7.19VHH68 pKa = 5.8YY69 pKa = 10.08

Molecular weight: 7.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

109

0

109

17977

35

1473

164.9

18.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.86 ± 0.603

1.163 ± 0.19

6.191 ± 0.247

6.92 ± 0.496

3.677 ± 0.194

6.736 ± 0.423

1.83 ± 0.166

5.763 ± 0.254

6.302 ± 0.384

7.287 ± 0.227

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.865 ± 0.209

5.674 ± 0.25

3.616 ± 0.173

4.127 ± 0.396

5.19 ± 0.285

6.837 ± 0.272

5.952 ± 0.294

7.02 ± 0.314

1.396 ± 0.132

3.593 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski