Arthrobacter phage Herb
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345KLY8|A0A345KLY8_9CAUD Helix-turn-helix DNA-binding domain protein OS=Arthrobacter phage Herb OX=2250411 GN=51 PE=4 SV=1
MM1 pKa = 7.68 INEE4 pKa = 4.75 AAPALIEE11 pKa = 3.84 QLNEE15 pKa = 3.53 VGAEE19 pKa = 3.88 LEE21 pKa = 4.6 RR22 pKa = 11.84 IHH24 pKa = 6.37 EE25 pKa = 4.65 AEE27 pKa = 4.19 GQEE30 pKa = 4.16 PPLTTGAIFLNVRR43 pKa = 11.84 TGRR46 pKa = 11.84 VWFQIQDD53 pKa = 3.32 QSYY56 pKa = 10.6 GSTLWVSKK64 pKa = 10.22 HH65 pKa = 5.32 EE66 pKa = 4.05 RR67 pKa = 11.84 HH68 pKa = 5.94 AAPGEE73 pKa = 4.13 TLVSVEE79 pKa = 4.32 SMEE82 pKa = 4.33 EE83 pKa = 3.99 AINYY87 pKa = 7.6 EE88 pKa = 3.6 WDD90 pKa = 3.05
Molecular weight: 10.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.512
IPC2_protein 4.495
IPC_protein 4.342
Toseland 4.228
ProMoST 4.329
Dawson 4.253
Bjellqvist 4.457
Wikipedia 4.075
Rodwell 4.19
Grimsley 4.139
Solomon 4.253
Lehninger 4.202
Nozaki 4.368
DTASelect 4.406
Thurlkill 4.202
EMBOSS 4.101
Sillero 4.444
Patrickios 3.694
IPC_peptide 4.266
IPC2_peptide 4.444
IPC2.peptide.svr19 4.368
Protein with the highest isoelectric point:
>tr|A0A345KLW8|A0A345KLW8_9CAUD Uncharacterized protein OS=Arthrobacter phage Herb OX=2250411 GN=31 PE=4 SV=1
MM1 pKa = 7.51 EE2 pKa = 4.95 RR3 pKa = 11.84 RR4 pKa = 11.84 NYY6 pKa = 9.74 NHH8 pKa = 7.12 PTSQRR13 pKa = 11.84 IPRR16 pKa = 11.84 KK17 pKa = 9.61 GKK19 pKa = 7.65 EE20 pKa = 3.99 LKK22 pKa = 10.75 GGAKK26 pKa = 10.21 SIRR29 pKa = 11.84 FTADD33 pKa = 2.2 YY34 pKa = 8.73 WAALRR39 pKa = 11.84 RR40 pKa = 11.84 RR41 pKa = 11.84 KK42 pKa = 9.78 QEE44 pKa = 3.67 AQSRR48 pKa = 11.84 LLQRR52 pKa = 11.84 QVRR55 pKa = 11.84 DD56 pKa = 3.14 HH57 pKa = 6.86 LRR59 pKa = 11.84 FLRR62 pKa = 11.84 EE63 pKa = 3.76 DD64 pKa = 3.88 DD65 pKa = 4.02 GRR67 pKa = 11.84 QPMYY71 pKa = 11.28 VFFGSGKK78 pKa = 9.28 EE79 pKa = 3.81 AEE81 pKa = 4.08 LWSRR85 pKa = 11.84 EE86 pKa = 3.76 FMLALLEE93 pKa = 4.38 VGEE96 pKa = 4.34 VWDD99 pKa = 4.54 PRR101 pKa = 11.84 KK102 pKa = 10.51 QMIQARR108 pKa = 11.84 IEE110 pKa = 3.88 RR111 pKa = 11.84 MRR113 pKa = 11.84 GHH115 pKa = 6.87 RR116 pKa = 11.84 AAWWRR121 pKa = 11.84 SGPRR125 pKa = 11.84 TPCRR129 pKa = 11.84 PPTTGTSRR137 pKa = 11.84 RR138 pKa = 11.84 SIRR141 pKa = 11.84 CTPLRR146 pKa = 11.84 TT147 pKa = 3.51
Molecular weight: 17.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.174
IPC2_protein 9.999
IPC_protein 11.359
Toseland 11.506
ProMoST 11.959
Dawson 11.52
Bjellqvist 11.491
Wikipedia 11.974
Rodwell 11.301
Grimsley 11.564
Solomon 11.974
Lehninger 11.886
Nozaki 11.506
DTASelect 11.491
Thurlkill 11.506
EMBOSS 12.003
Sillero 11.506
Patrickios 11.023
IPC_peptide 11.989
IPC2_peptide 10.965
IPC2.peptide.svr19 9.475
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
60
0
60
13985
39
877
233.1
25.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.197 ± 0.297
0.744 ± 0.128
5.72 ± 0.2
6.385 ± 0.39
3.018 ± 0.199
8.845 ± 0.372
1.859 ± 0.18
4.762 ± 0.316
4.87 ± 0.281
8.488 ± 0.317
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.295 ± 0.138
3.618 ± 0.185
4.998 ± 0.233
3.99 ± 0.176
5.821 ± 0.339
6.107 ± 0.317
6.471 ± 0.396
7.229 ± 0.27
2.045 ± 0.194
2.538 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here