Gordonia phage SheckWes

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A515MIJ2|A0A515MIJ2_9CAUD DNA helicase OS=Gordonia phage SheckWes OX=2591117 GN=60 PE=4 SV=1
MM1 pKa = 7.3NKK3 pKa = 10.1KK4 pKa = 10.18LIASIIFAFAIVAAPAGVAAASPCTIDD31 pKa = 3.67IPTGVAPCPPPVISHH46 pKa = 6.6YY47 pKa = 10.12GTDD50 pKa = 3.45PGGDD54 pKa = 3.1HH55 pKa = 7.13HH56 pKa = 6.7GTPDD60 pKa = 3.79QLDD63 pKa = 3.83PPAPNWQYY71 pKa = 11.26DD72 pKa = 3.62IPAPAADD79 pKa = 4.38DD80 pKa = 3.91EE81 pKa = 4.84VV82 pKa = 4.12

Molecular weight: 8.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A515MIE2|A0A515MIE2_9CAUD Portal protein OS=Gordonia phage SheckWes OX=2591117 GN=4 PE=4 SV=1
MM1 pKa = 7.66ANRR4 pKa = 11.84VRR6 pKa = 11.84LNHH9 pKa = 6.18KK10 pKa = 8.35GFRR13 pKa = 11.84AIRR16 pKa = 11.84SAPKK20 pKa = 10.02LVRR23 pKa = 11.84LEE25 pKa = 3.77EE26 pKa = 4.68RR27 pKa = 11.84IGKK30 pKa = 9.96GIADD34 pKa = 4.14RR35 pKa = 11.84ANAGLEE41 pKa = 3.69KK42 pKa = 10.44DD43 pKa = 3.88GYY45 pKa = 10.08IVEE48 pKa = 4.59SKK50 pKa = 10.45EE51 pKa = 3.88GAPRR55 pKa = 11.84PQGRR59 pKa = 11.84HH60 pKa = 4.16RR61 pKa = 11.84VTVYY65 pKa = 10.18TGTPEE70 pKa = 3.98AMKK73 pKa = 10.96DD74 pKa = 3.44NATHH78 pKa = 5.69NTLLRR83 pKa = 11.84EE84 pKa = 4.38FFNARR89 pKa = 11.84RR90 pKa = 3.69

Molecular weight: 10.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

15089

31

1697

201.2

22.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.842 ± 0.579

0.994 ± 0.129

6.72 ± 0.292

6.124 ± 0.345

3.108 ± 0.181

7.94 ± 0.59

2.068 ± 0.18

4.931 ± 0.164

4.898 ± 0.274

7.887 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.677 ± 0.12

3.168 ± 0.159

5.547 ± 0.285

3.877 ± 0.278

6.886 ± 0.325

5.428 ± 0.223

6.091 ± 0.347

6.959 ± 0.21

2.121 ± 0.158

2.737 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski