Capybara microvirus Cap3_SP_457
Average proteome isoelectric point is 5.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W8N8|A0A4P8W8N8_9VIRU Nonstructural protein OS=Capybara microvirus Cap3_SP_457 OX=2585461 PE=4 SV=1
MM1 pKa = 7.47 IKK3 pKa = 10.12 NRR5 pKa = 11.84 SAKK8 pKa = 10.29 DD9 pKa = 3.51 FPTNAGNAIKK19 pKa = 10.31 DD20 pKa = 3.52 VYY22 pKa = 10.68 RR23 pKa = 11.84 PIVQPDD29 pKa = 3.36 GSIILEE35 pKa = 4.29 AYY37 pKa = 9.56 EE38 pKa = 5.32 KK39 pKa = 10.28 IDD41 pKa = 3.62 LQAEE45 pKa = 4.2 IDD47 pKa = 3.93 SHH49 pKa = 8.9 FDD51 pKa = 3.32 EE52 pKa = 7.08 CDD54 pKa = 2.57 MTAIVNRR61 pKa = 11.84 LMQGDD66 pKa = 4.05 LSDD69 pKa = 4.17 FQRR72 pKa = 11.84 SNPLYY77 pKa = 9.06 MDD79 pKa = 3.13 STVFPKK85 pKa = 10.23 TYY87 pKa = 11.14 AEE89 pKa = 3.99 MLQLQIDD96 pKa = 3.84 VDD98 pKa = 3.99 QYY100 pKa = 10.83 FASLPTEE107 pKa = 3.97 VKK109 pKa = 8.78 EE110 pKa = 4.21 TYY112 pKa = 10.44 GYY114 pKa = 11.01 DD115 pKa = 3.32 RR116 pKa = 11.84 NKK118 pKa = 9.82 FFAAIGSDD126 pKa = 2.95 EE127 pKa = 4.06 WFKK130 pKa = 11.62 VLGISNPAAAAVTSEE145 pKa = 4.39 EE146 pKa = 4.53 GVSEE150 pKa = 4.15
Molecular weight: 16.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.35
IPC2_protein 4.279
IPC_protein 4.228
Toseland 4.037
ProMoST 4.317
Dawson 4.19
Bjellqvist 4.38
Wikipedia 4.101
Rodwell 4.05
Grimsley 3.935
Solomon 4.19
Lehninger 4.139
Nozaki 4.304
DTASelect 4.507
Thurlkill 4.062
EMBOSS 4.113
Sillero 4.342
Patrickios 3.681
IPC_peptide 4.19
IPC2_peptide 4.317
IPC2.peptide.svr19 4.273
Protein with the highest isoelectric point:
>tr|A0A4P8W676|A0A4P8W676_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_457 OX=2585461 PE=4 SV=1
MM1 pKa = 7.68 RR2 pKa = 11.84 CIKK5 pKa = 10.71 LMLNTMVVRR14 pKa = 11.84 FRR16 pKa = 11.84 CSIDD20 pKa = 3.94 DD21 pKa = 3.66 YY22 pKa = 11.56 DD23 pKa = 3.58 ILRR26 pKa = 11.84 RR27 pKa = 11.84 EE28 pKa = 3.8 AAKK31 pKa = 10.75 RR32 pKa = 11.84 NMNMSEE38 pKa = 4.22 LFRR41 pKa = 11.84 FLIRR45 pKa = 11.84 RR46 pKa = 11.84 EE47 pKa = 4.06 ALSDD51 pKa = 3.35 DD52 pKa = 3.84 CNIIEE57 pKa = 4.24 EE58 pKa = 4.34 VQIKK62 pKa = 10.34 DD63 pKa = 3.32 CSNYY67 pKa = 10.34 DD68 pKa = 3.34 VRR70 pKa = 11.84 CC71 pKa = 3.61
Molecular weight: 8.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.747
IPC2_protein 6.707
IPC_protein 6.942
Toseland 6.3
ProMoST 7.512
Dawson 7.585
Bjellqvist 7.6
Wikipedia 7.483
Rodwell 7.556
Grimsley 6.211
Solomon 7.688
Lehninger 7.717
Nozaki 8.024
DTASelect 7.688
Thurlkill 7.717
EMBOSS 7.805
Sillero 8.083
Patrickios 4.266
IPC_peptide 7.688
IPC2_peptide 7.556
IPC2.peptide.svr19 7.511
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1539
71
559
219.9
24.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.992 ± 1.99
1.624 ± 0.575
7.147 ± 0.461
4.418 ± 0.89
5.003 ± 0.468
5.913 ± 0.887
2.404 ± 0.678
4.418 ± 0.817
4.808 ± 1.066
8.902 ± 0.957
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.534 ± 0.539
4.094 ± 0.938
4.808 ± 0.669
4.224 ± 0.321
4.613 ± 0.793
9.747 ± 1.429
4.353 ± 0.462
6.303 ± 0.215
1.235 ± 0.272
5.458 ± 0.357
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here