Torque teno virus (isolate Chimpanzee/Japan/Pt-TTV6/2000) (TTV)
Average proteome isoelectric point is 8.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q9DUD0|ORF2_TTVV8 Uncharacterized ORF2 protein OS=Torque teno virus (isolate Chimpanzee/Japan/Pt-TTV6/2000) OX=687343 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.45 HH2 pKa = 7.29 FSKK5 pKa = 10.89 LSRR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.51 KK11 pKa = 10.58 GKK13 pKa = 10.24 VPLPPLPTAPLPPHH27 pKa = 7.05 RR28 pKa = 11.84 PRR30 pKa = 11.84 MSGTWCPPVHH40 pKa = 6.17 NVPGLEE46 pKa = 4.12 RR47 pKa = 11.84 NWYY50 pKa = 7.82 EE51 pKa = 3.69 SCLRR55 pKa = 11.84 SHH57 pKa = 7.32 AAFCGCGDD65 pKa = 4.38 FVSHH69 pKa = 7.22 LNNLANRR76 pKa = 11.84 LGRR79 pKa = 11.84 PPAPRR84 pKa = 11.84 PPGAPQPPAVRR95 pKa = 11.84 ALPALPAPEE104 pKa = 5.17 DD105 pKa = 3.74 RR106 pKa = 11.84 FPNPPGWPGPGGGAAGAGAAAGRR129 pKa = 11.84 DD130 pKa = 3.72 GGDD133 pKa = 3.59 GGDD136 pKa = 4.33 AEE138 pKa = 5.32 PGDD141 pKa = 4.43 EE142 pKa = 5.76 DD143 pKa = 5.86 LDD145 pKa = 3.88 ALFAADD151 pKa = 3.7 EE152 pKa = 4.25
Molecular weight: 15.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.896
IPC2_protein 6.912
IPC_protein 7.132
Toseland 6.898
ProMoST 7.234
Dawson 7.717
Bjellqvist 7.746
Wikipedia 7.644
Rodwell 7.702
Grimsley 6.956
Solomon 7.849
Lehninger 7.878
Nozaki 8.17
DTASelect 7.863
Thurlkill 7.907
EMBOSS 7.995
Sillero 8.2
Patrickios 4.673
IPC_peptide 7.849
IPC2_peptide 7.629
IPC2.peptide.svr19 7.437
Protein with the highest isoelectric point:
>sp|Q9DUD0|ORF2_TTVV8 Uncharacterized ORF2 protein OS=Torque teno virus (isolate Chimpanzee/Japan/Pt-TTV6/2000) OX=687343 GN=ORF2 PE=4 SV=1
MM1 pKa = 7.33 AWPWRR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 WRR11 pKa = 11.84 WRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 RR16 pKa = 11.84 PRR18 pKa = 11.84 WRR20 pKa = 11.84 RR21 pKa = 11.84 WRR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 RR26 pKa = 11.84 TWRR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 PRR33 pKa = 11.84 RR34 pKa = 11.84 AVRR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 VRR42 pKa = 11.84 RR43 pKa = 11.84 RR44 pKa = 11.84 GRR46 pKa = 11.84 GWRR49 pKa = 11.84 RR50 pKa = 11.84 LYY52 pKa = 10.01 RR53 pKa = 11.84 RR54 pKa = 11.84 YY55 pKa = 9.72 RR56 pKa = 11.84 RR57 pKa = 11.84 RR58 pKa = 11.84 RR59 pKa = 11.84 GRR61 pKa = 11.84 RR62 pKa = 11.84 TYY64 pKa = 10.38 KK65 pKa = 10.19 KK66 pKa = 10.56 KK67 pKa = 10.23 PVLTQWQPTVRR78 pKa = 11.84 RR79 pKa = 11.84 RR80 pKa = 11.84 CFIIGYY86 pKa = 8.07 MPLIICGEE94 pKa = 4.01 NLTSKK99 pKa = 10.65 NYY101 pKa = 10.32 ASHH104 pKa = 7.18 ADD106 pKa = 3.85 DD107 pKa = 4.11 FVKK110 pKa = 10.66 DD111 pKa = 3.94 GPFGGGMTTMQFSLRR126 pKa = 11.84 ILYY129 pKa = 10.41 DD130 pKa = 3.14 EE131 pKa = 4.94 FLRR134 pKa = 11.84 FLNIWTHH141 pKa = 5.67 SNQDD145 pKa = 3.02 LDD147 pKa = 3.6 LARR150 pKa = 11.84 YY151 pKa = 9.15 HH152 pKa = 6.45 GFKK155 pKa = 9.77 LTLYY159 pKa = 9.52 RR160 pKa = 11.84 HH161 pKa = 5.57 PTVDD165 pKa = 4.47 FIVIIRR171 pKa = 11.84 NSPPFEE177 pKa = 4.31 DD178 pKa = 4.61 TEE180 pKa = 4.32 LTGPNTHH187 pKa = 7.26 PGMLMLRR194 pKa = 11.84 HH195 pKa = 6.04 KK196 pKa = 10.36 KK197 pKa = 9.84 ILVPSLRR204 pKa = 11.84 TRR206 pKa = 11.84 PSRR209 pKa = 11.84 RR210 pKa = 11.84 HH211 pKa = 6.05 KK212 pKa = 8.25 ITVRR216 pKa = 11.84 IGPPKK221 pKa = 10.27 LFEE224 pKa = 5.05 DD225 pKa = 3.23 KK226 pKa = 10.07 WYY228 pKa = 10.35 SQTDD232 pKa = 3.08 ICDD235 pKa = 3.6 VILATVYY242 pKa = 9.35 ATACDD247 pKa = 3.58 LQYY250 pKa = 11.25 PFGSPLTEE258 pKa = 3.94 NYY260 pKa = 10.05 CISFQVLGSAYY271 pKa = 10.89 NNLISNTLNPNEE283 pKa = 4.08 EE284 pKa = 4.01 QQDD287 pKa = 3.57 IKK289 pKa = 11.23 SAIYY293 pKa = 10.74 NNVNAYY299 pKa = 7.34 LTRR302 pKa = 11.84 ITEE305 pKa = 3.9 SHH307 pKa = 5.93 MANLISKK314 pKa = 9.52 APPKK318 pKa = 10.15 QVLKK322 pKa = 11.05 SSDD325 pKa = 3.11 GSLQTDD331 pKa = 3.34 HH332 pKa = 7.29 NDD334 pKa = 3.26 TQFGGNPYY342 pKa = 8.64 NTNQFTTTTVNKK354 pKa = 9.98 IIQGAQNYY362 pKa = 7.11 LTTIKK367 pKa = 9.04 THH369 pKa = 5.96 LQPNNSAINPNTQWHH384 pKa = 6.54 LEE386 pKa = 4.03 YY387 pKa = 10.58 HH388 pKa = 6.12 AGIYY392 pKa = 9.01 SAPFLSAGRR401 pKa = 11.84 LNPEE405 pKa = 3.63 IKK407 pKa = 10.51 GLYY410 pKa = 8.9 TDD412 pKa = 3.47 ITYY415 pKa = 11.14 NPMMDD420 pKa = 3.55 KK421 pKa = 10.51 GTGNKK426 pKa = 8.6 IWCDD430 pKa = 3.57 SLTKK434 pKa = 10.59 ADD436 pKa = 3.53 MKK438 pKa = 9.87 YY439 pKa = 8.58 TEE441 pKa = 4.69 GRR443 pKa = 11.84 SKK445 pKa = 11.14 YY446 pKa = 10.55 LIEE449 pKa = 5.41 NLPLWAAVWGYY460 pKa = 11.45 LDD462 pKa = 4.26 YY463 pKa = 10.3 CTKK466 pKa = 10.36 TSGDD470 pKa = 3.28 AAFHH474 pKa = 5.35 YY475 pKa = 10.1 NYY477 pKa = 9.99 RR478 pKa = 11.84 VTLISPYY485 pKa = 9.05 TSPMLFNPQDD495 pKa = 3.59 PTKK498 pKa = 10.95 GFVPYY503 pKa = 10.45 SLNFGLGKK511 pKa = 9.64 MPGGKK516 pKa = 9.99 GYY518 pKa = 11.01 VPLRR522 pKa = 11.84 MRR524 pKa = 11.84 ANWYY528 pKa = 9.35 PYY530 pKa = 9.91 FFHH533 pKa = 5.6 QQKK536 pKa = 9.66 VLEE539 pKa = 5.0 AIGMSGPFTYY549 pKa = 10.41 RR550 pKa = 11.84 SDD552 pKa = 3.41 EE553 pKa = 4.16 KK554 pKa = 11.08 KK555 pKa = 10.53 AVLTSRR561 pKa = 11.84 YY562 pKa = 9.32 KK563 pKa = 10.85 FKK565 pKa = 10.27 FTWGGNPVSHH575 pKa = 5.32 QVVRR579 pKa = 11.84 NPCKK583 pKa = 9.65 GTGGASASRR592 pKa = 11.84 KK593 pKa = 7.95 PRR595 pKa = 11.84 SVQVTDD601 pKa = 3.86 PKK603 pKa = 11.35 YY604 pKa = 8.12 NTPEE608 pKa = 4.03 ITTHH612 pKa = 4.32 TWDD615 pKa = 4.03 IRR617 pKa = 11.84 RR618 pKa = 11.84 GWFGKK623 pKa = 10.35 RR624 pKa = 11.84 FIDD627 pKa = 5.16 RR628 pKa = 11.84 VQQQQASPEE637 pKa = 4.16 LLADD641 pKa = 3.68 PPKK644 pKa = 10.55 RR645 pKa = 11.84 PRR647 pKa = 11.84 KK648 pKa = 8.7 EE649 pKa = 3.73 IKK651 pKa = 10.41 GLTEE655 pKa = 4.36 ADD657 pKa = 3.25 QEE659 pKa = 4.3 AEE661 pKa = 4.01 KK662 pKa = 11.11 DD663 pKa = 3.17 SGLRR667 pKa = 11.84 LRR669 pKa = 11.84 QVQPWMSSQEE679 pKa = 4.2 TQSEE683 pKa = 4.47 QEE685 pKa = 4.33 SAPEE689 pKa = 3.96 EE690 pKa = 4.13 QTVEE694 pKa = 3.91 QQLRR698 pKa = 11.84 NQLHH702 pKa = 4.85 TQQLLGFQLRR712 pKa = 11.84 SLMYY716 pKa = 9.84 QVQQTHH722 pKa = 6.6 RR723 pKa = 11.84 NSFIHH728 pKa = 6.81 PLLLPRR734 pKa = 11.84 AA735 pKa = 4.06
Molecular weight: 86.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.312
IPC2_protein 9.75
IPC_protein 10.394
Toseland 10.35
ProMoST 10.101
Dawson 10.54
Bjellqvist 10.262
Wikipedia 10.745
Rodwell 10.804
Grimsley 10.628
Solomon 10.584
Lehninger 10.54
Nozaki 10.35
DTASelect 10.248
Thurlkill 10.394
EMBOSS 10.76
Sillero 10.467
Patrickios 10.365
IPC_peptide 10.584
IPC2_peptide 9.18
IPC2.peptide.svr19 8.61
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
887
152
735
443.5
50.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.088 ± 3.163
1.353 ± 0.572
4.397 ± 0.682
3.72 ± 0.102
3.72 ± 0.193
7.328 ± 2.608
2.706 ± 0.261
4.059 ± 1.816
4.961 ± 1.042
8.23 ± 0.144
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.029 ± 0.319
5.073 ± 0.504
8.794 ± 4.307
4.848 ± 1.874
9.921 ± 0.906
5.411 ± 0.655
6.652 ± 2.387
3.946 ± 0.294
2.593 ± 0.277
4.171 ± 1.572
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here