Plasmodium yoelii yoelii
Average proteome isoelectric point is 7.26
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7741 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q7RHP3|Q7RHP3_PLAYO GAF domain protein OS=Plasmodium yoelii yoelii OX=73239 GN=PY03941 PE=4 SV=1
MM1 pKa = 8.31 DD2 pKa = 4.66 DD3 pKa = 3.95 TEE5 pKa = 5.24 GLAPGNMNDD14 pKa = 4.75 AEE16 pKa = 4.66 GLAPDD21 pKa = 4.95 NMDD24 pKa = 3.8 DD25 pKa = 4.35 TEE27 pKa = 4.89 GLAPGNMDD35 pKa = 3.25 DD36 pKa = 4.65 TEE38 pKa = 4.62 HH39 pKa = 5.4 NHH41 pKa = 4.09 YY42 pKa = 10.22 RR43 pKa = 11.84 YY44 pKa = 10.74 YY45 pKa = 10.27 IDD47 pKa = 4.13 IRR49 pKa = 11.84 LIHH52 pKa = 5.89 YY53 pKa = 8.55
Molecular weight: 6.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.884
IPC_protein 3.821
Toseland 3.617
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.808
Rodwell 3.656
Grimsley 3.528
Solomon 3.808
Lehninger 3.77
Nozaki 3.961
DTASelect 4.202
Thurlkill 3.694
EMBOSS 3.808
Sillero 3.948
Patrickios 0.769
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.84
Protein with the highest isoelectric point:
>tr|Q7RAX0|Q7RAX0_PLAYO N-acetylglucosaminylphosphatidylinositol deacetylase (Fragment) OS=Plasmodium yoelii yoelii OX=73239 GN=PY06379 PE=3 SV=1
SS1 pKa = 6.69 SRR3 pKa = 11.84 FIPRR7 pKa = 11.84 FTRR10 pKa = 11.84 RR11 pKa = 11.84 IKK13 pKa = 10.6 KK14 pKa = 10.15 NKK16 pKa = 9.12
Molecular weight: 2.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 10.76
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.281
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.047
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.065
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7741
0
7741
3378133
8
6636
436.4
51.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.514 ± 0.025
1.745 ± 0.013
5.929 ± 0.023
7.104 ± 0.04
4.785 ± 0.027
3.375 ± 0.027
1.93 ± 0.009
9.646 ± 0.031
11.509 ± 0.04
7.873 ± 0.033
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.909 ± 0.01
12.799 ± 0.072
2.276 ± 0.021
2.797 ± 0.029
2.655 ± 0.021
7.201 ± 0.026
4.298 ± 0.017
3.717 ± 0.021
0.521 ± 0.007
5.366 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here