Mesobacillus persicus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Mesobacillus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4551 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H7WBZ5|A0A1H7WBZ5_9BACI Branched-chain amino acid transport system permease protein OS=Mesobacillus persicus OX=930146 GN=SAMN05192533_101357 PE=4 SV=1
MM1 pKa = 7.73AEE3 pKa = 4.15FMDD6 pKa = 5.19SIDD9 pKa = 3.82YY10 pKa = 10.69EE11 pKa = 3.98IGVPIRR17 pKa = 11.84TFNILDD23 pKa = 3.53TSVTEE28 pKa = 3.95EE29 pKa = 4.65GYY31 pKa = 10.69EE32 pKa = 3.89VLLNIDD38 pKa = 4.02LDD40 pKa = 3.95SGYY43 pKa = 10.69EE44 pKa = 3.93LEE46 pKa = 4.29DD47 pKa = 3.28VKK49 pKa = 10.99MKK51 pKa = 11.01LSFEE55 pKa = 4.33DD56 pKa = 3.47LAGYY60 pKa = 7.2EE61 pKa = 4.27TEE63 pKa = 4.79DD64 pKa = 3.11VHH66 pKa = 8.51EE67 pKa = 4.48GVKK70 pKa = 10.49YY71 pKa = 10.67ALGFFAEE78 pKa = 4.43

Molecular weight: 8.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H7YJ58|A0A1H7YJ58_9BACI Uracil permease OS=Mesobacillus persicus OX=930146 GN=SAMN05192533_10318 PE=3 SV=1
MM1 pKa = 8.17RR2 pKa = 11.84YY3 pKa = 9.79LFLAIVLIPAIEE15 pKa = 3.89IAILLLVGQTIGVPLTFLIIIATGILGAYY44 pKa = 8.53LAKK47 pKa = 10.3QQGLAAIRR55 pKa = 11.84NVQDD59 pKa = 3.18RR60 pKa = 11.84LQRR63 pKa = 11.84GSMPGDD69 pKa = 3.48ALLDD73 pKa = 4.63GICILIGGTLLLTPGFVTDD92 pKa = 4.41LVGFLLLAPPTRR104 pKa = 11.84NYY106 pKa = 9.83FKK108 pKa = 10.71AWLVRR113 pKa = 11.84IFRR116 pKa = 11.84NRR118 pKa = 11.84IDD120 pKa = 3.87RR121 pKa = 11.84GTITIIRR128 pKa = 4.02

Molecular weight: 14.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4551

0

4551

1269223

29

2068

278.9

31.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.969 ± 0.038

0.719 ± 0.011

5.028 ± 0.028

7.813 ± 0.045

4.586 ± 0.034

7.118 ± 0.035

2.046 ± 0.017

7.627 ± 0.03

6.777 ± 0.032

9.868 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.756 ± 0.016

4.373 ± 0.026

3.68 ± 0.021

3.668 ± 0.026

4.107 ± 0.027

5.93 ± 0.028

5.392 ± 0.022

7.108 ± 0.034

1.013 ± 0.014

3.421 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski