Hubei sobemo-like virus 31

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEU2|A0A1L3KEU2_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 31 OX=1923218 PE=4 SV=1
MM1 pKa = 7.42RR2 pKa = 11.84GEE4 pKa = 4.1RR5 pKa = 11.84AEE7 pKa = 4.17LTSLLYY13 pKa = 10.24HH14 pKa = 6.23AQLRR18 pKa = 11.84DD19 pKa = 3.85DD20 pKa = 4.05LVKK23 pKa = 10.88SITEE27 pKa = 3.95HH28 pKa = 6.03EE29 pKa = 4.29KK30 pKa = 11.21GILLNKK36 pKa = 10.33LEE38 pKa = 4.1MQFRR42 pKa = 11.84HH43 pKa = 6.06AKK45 pKa = 7.11WTIPSDD51 pKa = 3.51FMKK54 pKa = 10.29RR55 pKa = 11.84SHH57 pKa = 7.4FDD59 pKa = 3.11RR60 pKa = 11.84VLKK63 pKa = 10.75NIDD66 pKa = 3.43MTSSPGYY73 pKa = 9.63PYY75 pKa = 11.4LLVHH79 pKa = 6.5TNNASFFNCKK89 pKa = 10.0EE90 pKa = 3.72GVLDD94 pKa = 4.83VEE96 pKa = 5.13RR97 pKa = 11.84ADD99 pKa = 4.36QIWNRR104 pKa = 11.84VNSYY108 pKa = 10.43IEE110 pKa = 4.45NKK112 pKa = 10.4LSDD115 pKa = 4.82PIRR118 pKa = 11.84LFVKK122 pKa = 10.06PEE124 pKa = 3.61PHH126 pKa = 6.59NEE128 pKa = 3.35RR129 pKa = 11.84KK130 pKa = 8.74MRR132 pKa = 11.84SGRR135 pKa = 11.84YY136 pKa = 8.78RR137 pKa = 11.84LISSVSIIDD146 pKa = 3.78QIIDD150 pKa = 3.12AMLFGEE156 pKa = 4.48MNEE159 pKa = 3.96IMIHH163 pKa = 5.35NWPMLPTRR171 pKa = 11.84VGWSPYY177 pKa = 8.94YY178 pKa = 10.62GGWKK182 pKa = 9.11QMPYY186 pKa = 11.0AKK188 pKa = 9.21TQMAIDD194 pKa = 3.54KK195 pKa = 7.54TAWDD199 pKa = 3.66WSVQGWLPEE208 pKa = 3.81IVLEE212 pKa = 4.59LRR214 pKa = 11.84KK215 pKa = 9.82RR216 pKa = 11.84LCRR219 pKa = 11.84NLTSDD224 pKa = 4.0WEE226 pKa = 4.2RR227 pKa = 11.84LANWRR232 pKa = 11.84YY233 pKa = 9.44KK234 pKa = 10.98ALFGNPTLVTSGGLFLRR251 pKa = 11.84QKK253 pKa = 10.47QSGVMKK259 pKa = 9.96SGCYY263 pKa = 7.83NTIIDD268 pKa = 4.11NSIMQIVLHH277 pKa = 6.79DD278 pKa = 4.65LVCQTLNIPIGSIIAMGDD296 pKa = 3.33DD297 pKa = 3.77TLQDD301 pKa = 3.27ALFGEE306 pKa = 4.37EE307 pKa = 3.76RR308 pKa = 11.84EE309 pKa = 4.33RR310 pKa = 11.84YY311 pKa = 9.54LEE313 pKa = 4.07EE314 pKa = 3.79LSRR317 pKa = 11.84YY318 pKa = 10.15CNVKK322 pKa = 9.21VCNVSNEE329 pKa = 3.87FCGMHH334 pKa = 6.21FNIDD338 pKa = 3.32GRR340 pKa = 11.84VEE342 pKa = 3.56PVYY345 pKa = 10.23RR346 pKa = 11.84AKK348 pKa = 10.39HH349 pKa = 5.35AYY351 pKa = 8.94KK352 pKa = 9.96ILYY355 pKa = 8.61MDD357 pKa = 5.24DD358 pKa = 4.51SISDD362 pKa = 3.79SMADD366 pKa = 3.53SYY368 pKa = 11.84TLMYY372 pKa = 9.91YY373 pKa = 10.44RR374 pKa = 11.84SRR376 pKa = 11.84LKK378 pKa = 10.82DD379 pKa = 4.03LIDD382 pKa = 3.42SLFRR386 pKa = 11.84KK387 pKa = 8.5MGCEE391 pKa = 3.9VRR393 pKa = 11.84SDD395 pKa = 3.73EE396 pKa = 4.21YY397 pKa = 11.19RR398 pKa = 11.84DD399 pKa = 3.93AIFGG403 pKa = 3.75

Molecular weight: 47.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEU2|A0A1L3KEU2_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 31 OX=1923218 PE=4 SV=1
MM1 pKa = 7.3YY2 pKa = 10.64NVIDD6 pKa = 4.07AFTYY10 pKa = 10.68AVLGYY15 pKa = 10.44AATQAPGLSLAADD28 pKa = 4.11VIWRR32 pKa = 11.84SIKK35 pKa = 9.63WLFNVSFRR43 pKa = 11.84EE44 pKa = 3.93TKK46 pKa = 10.36AYY48 pKa = 9.87LKK50 pKa = 10.35PPTRR54 pKa = 11.84RR55 pKa = 11.84EE56 pKa = 3.98VMRR59 pKa = 11.84DD60 pKa = 2.55WANRR64 pKa = 11.84LVEE67 pKa = 4.55RR68 pKa = 11.84GLAEE72 pKa = 3.86INAIQALEE80 pKa = 3.79WSIIGLVLVLLVLVWKK96 pKa = 10.32RR97 pKa = 11.84GRR99 pKa = 11.84RR100 pKa = 11.84TVHH103 pKa = 7.11RR104 pKa = 11.84IRR106 pKa = 11.84GIKK109 pKa = 9.07YY110 pKa = 7.75EE111 pKa = 3.96AMMANSHH118 pKa = 4.82FHH120 pKa = 5.99EE121 pKa = 5.3GKK123 pKa = 9.68PLEE126 pKa = 4.43CQVAILIPGLLTDD139 pKa = 3.66KK140 pKa = 11.1HH141 pKa = 6.39NGYY144 pKa = 9.83GFRR147 pKa = 11.84VEE149 pKa = 5.07DD150 pKa = 3.94FLVVPKK156 pKa = 10.57HH157 pKa = 4.9VVAGKK162 pKa = 10.43DD163 pKa = 3.34EE164 pKa = 4.31IMLKK168 pKa = 10.55NLFFPEE174 pKa = 4.13KK175 pKa = 10.43KK176 pKa = 10.74LMISTAGRR184 pKa = 11.84IDD186 pKa = 3.38SKK188 pKa = 11.69LNVDD192 pKa = 4.28LSYY195 pKa = 11.42ILLTKK200 pKa = 10.46KK201 pKa = 9.71SWSTVGVSKK210 pKa = 10.97SSIIAGTKK218 pKa = 10.35SLMQSVTIYY227 pKa = 10.44GPKK230 pKa = 10.01GVSEE234 pKa = 4.01GALRR238 pKa = 11.84HH239 pKa = 5.47TNMIGQLAYY248 pKa = 9.47TGSTIPGMSGSVYY261 pKa = 8.8TSEE264 pKa = 3.9GRR266 pKa = 11.84VLGMHH271 pKa = 6.66TGAFEE276 pKa = 4.03KK277 pKa = 10.5KK278 pKa = 10.05INVGLAGSMIKK289 pKa = 10.55EE290 pKa = 3.94EE291 pKa = 4.32VKK293 pKa = 10.9YY294 pKa = 9.36LTVVQLEE301 pKa = 4.29SSEE304 pKa = 4.22EE305 pKa = 3.99LLEE308 pKa = 4.23VAADD312 pKa = 3.53NGDD315 pKa = 3.6WNDD318 pKa = 3.17AMMNGYY324 pKa = 10.53VNDD327 pKa = 4.51KK328 pKa = 10.38YY329 pKa = 10.65SQRR332 pKa = 11.84QYY334 pKa = 11.61EE335 pKa = 3.98LDD337 pKa = 3.35QRR339 pKa = 11.84YY340 pKa = 9.57LEE342 pKa = 4.46DD343 pKa = 3.48KK344 pKa = 10.07KK345 pKa = 11.25AKK347 pKa = 10.08EE348 pKa = 4.35EE349 pKa = 4.02EE350 pKa = 3.94EE351 pKa = 3.82KK352 pKa = 10.79AYY354 pKa = 9.7TKK356 pKa = 10.54RR357 pKa = 11.84FYY359 pKa = 11.42ARR361 pKa = 11.84LASGEE366 pKa = 3.97IDD368 pKa = 4.05DD369 pKa = 4.15WTSDD373 pKa = 3.64DD374 pKa = 4.56FLGEE378 pKa = 4.15SAIKK382 pKa = 10.34LARR385 pKa = 11.84QLVEE389 pKa = 3.5RR390 pKa = 11.84ADD392 pKa = 4.1RR393 pKa = 11.84KK394 pKa = 10.65KK395 pKa = 9.95KK396 pKa = 8.48TIKK399 pKa = 10.66AEE401 pKa = 4.04SSDD404 pKa = 4.07GEE406 pKa = 4.72DD407 pKa = 4.66IPLQDD412 pKa = 3.02MALYY416 pKa = 10.33RR417 pKa = 11.84RR418 pKa = 11.84LTDD421 pKa = 3.14MEE423 pKa = 4.04ATIEE427 pKa = 3.99RR428 pKa = 11.84LAEE431 pKa = 3.66RR432 pKa = 11.84LAFVEE437 pKa = 4.16NRR439 pKa = 11.84LCRR442 pKa = 11.84EE443 pKa = 3.67TAMGQVVGYY452 pKa = 10.19RR453 pKa = 11.84CDD455 pKa = 3.3EE456 pKa = 4.26CRR458 pKa = 11.84TSCTTAEE465 pKa = 4.15KK466 pKa = 11.03LEE468 pKa = 4.04NHH470 pKa = 6.6KK471 pKa = 10.54KK472 pKa = 9.28AAHH475 pKa = 6.14PKK477 pKa = 8.74VEE479 pKa = 4.58KK480 pKa = 10.55YY481 pKa = 9.28PCPSCDD487 pKa = 3.42TVCNTQAKK495 pKa = 9.62LSNHH499 pKa = 5.75MLSNHH504 pKa = 5.51IPKK507 pKa = 9.72IEE509 pKa = 4.52LYY511 pKa = 10.16QCPLCNDD518 pKa = 3.58RR519 pKa = 11.84LEE521 pKa = 5.25KK522 pKa = 9.94EE523 pKa = 4.61ALMQHH528 pKa = 6.31ISKK531 pKa = 9.16HH532 pKa = 4.22QPEE535 pKa = 4.29AAEE538 pKa = 3.95EE539 pKa = 4.01AKK541 pKa = 10.67EE542 pKa = 3.8AVAFLGRR549 pKa = 11.84EE550 pKa = 3.69RR551 pKa = 11.84SSNQSSRR558 pKa = 11.84NGSTSWSAEE567 pKa = 3.75RR568 pKa = 11.84PRR570 pKa = 11.84QADD573 pKa = 3.08QPSPYY578 pKa = 10.08RR579 pKa = 11.84STTEE583 pKa = 3.62YY584 pKa = 10.82LKK586 pKa = 10.77KK587 pKa = 10.43KK588 pKa = 9.7KK589 pKa = 9.67PSSGKK594 pKa = 8.77PQQ596 pKa = 3.09

Molecular weight: 67.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

999

403

596

499.5

57.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.707 ± 1.39

1.802 ± 0.102

5.405 ± 0.723

7.407 ± 0.811

2.703 ± 0.431

5.806 ± 0.194

2.302 ± 0.1

5.906 ± 0.721

7.007 ± 0.865

9.409 ± 0.15

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.704 ± 0.565

4.304 ± 0.507

3.504 ± 0.017

3.203 ± 0.126

6.807 ± 0.356

7.508 ± 0.035

4.605 ± 0.493

5.806 ± 0.332

1.902 ± 0.324

4.204 ± 0.285
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski