Beihai sphaeromadae virus 3
Average proteome isoelectric point is 7.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFI2|A0A1L3KFI2_9VIRU Capsid protein OS=Beihai sphaeromadae virus 3 OX=1922709 PE=4 SV=1
MM1 pKa = 8.01 ANGKK5 pKa = 8.94 QNNQRR10 pKa = 11.84 RR11 pKa = 11.84 RR12 pKa = 11.84 VPPNPNPPKK21 pKa = 9.85 PRR23 pKa = 11.84 ANRR26 pKa = 11.84 APRR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 NMRR34 pKa = 11.84 PSNGNVTLVGANQALQRR51 pKa = 11.84 YY52 pKa = 7.03 NNPTGGHH59 pKa = 5.88 RR60 pKa = 11.84 QTGSDD65 pKa = 4.07 LLTAITIQEE74 pKa = 4.24 TVNAGEE80 pKa = 4.47 LLADD84 pKa = 4.32 IIITPSVAARR94 pKa = 11.84 LEE96 pKa = 4.02 VLAAAWQRR104 pKa = 11.84 LRR106 pKa = 11.84 YY107 pKa = 9.53 NRR109 pKa = 11.84 LTFEE113 pKa = 4.5 VNASASSIVGGEE125 pKa = 3.68 FVAAFVSDD133 pKa = 3.72 PTDD136 pKa = 3.67 RR137 pKa = 11.84 PPLKK141 pKa = 10.84 DD142 pKa = 2.91 ADD144 pKa = 3.78 KK145 pKa = 10.27 WVKK148 pKa = 10.29 AHH150 pKa = 6.85 AGSITSSWWKK160 pKa = 10.14 SMNVRR165 pKa = 11.84 GPCPPQMMYY174 pKa = 10.62 TSFEE178 pKa = 4.22 EE179 pKa = 4.04 NEE181 pKa = 4.17 PRR183 pKa = 11.84 FSSPGRR189 pKa = 11.84 FVVAVVNPPTSAATMSISLSWDD211 pKa = 3.1 VSFTQPSLEE220 pKa = 4.15 TFIEE224 pKa = 4.51 DD225 pKa = 4.13 DD226 pKa = 3.6 NNIYY230 pKa = 10.03 IVNASSRR237 pKa = 11.84 LPISDD242 pKa = 3.89 GSAGQTFNPYY252 pKa = 10.23 LIKK255 pKa = 10.73 EE256 pKa = 4.22 EE257 pKa = 4.31 SDD259 pKa = 3.57 GSFNDD264 pKa = 3.48 NDD266 pKa = 5.22 PITNYY271 pKa = 10.28 LAATDD276 pKa = 4.25 FTPEE280 pKa = 4.17 LPDD283 pKa = 3.87 GVLLQLPHH291 pKa = 7.28 PKK293 pKa = 9.06 TLNSDD298 pKa = 3.77 TGASGAPEE306 pKa = 3.95 NAIVTHH312 pKa = 6.55 IGVADD317 pKa = 3.66 GAGRR321 pKa = 11.84 IGYY324 pKa = 7.37 FLRR327 pKa = 11.84 TEE329 pKa = 3.72 ADD331 pKa = 3.65 YY332 pKa = 10.7 FQLLAGFPFSIRR344 pKa = 11.84 PVVDD348 pKa = 4.68 SINQAGTIYY357 pKa = 10.79 DD358 pKa = 3.83 GEE360 pKa = 4.45 AEE362 pKa = 4.27 NEE364 pKa = 4.4 VATLAAPSRR373 pKa = 11.84 GRR375 pKa = 11.84 RR376 pKa = 11.84 SLTSGPVLSRR386 pKa = 11.84 SSKK389 pKa = 10.53 GRR391 pKa = 11.84 MLSLLNSKK399 pKa = 10.26 RR400 pKa = 11.84 FF401 pKa = 3.42
Molecular weight: 43.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.992
IPC2_protein 7.146
IPC_protein 7.688
Toseland 7.775
ProMoST 7.629
Dawson 8.156
Bjellqvist 7.834
Wikipedia 8.141
Rodwell 8.097
Grimsley 7.614
Solomon 8.507
Lehninger 8.492
Nozaki 8.024
DTASelect 8.112
Thurlkill 8.141
EMBOSS 8.404
Sillero 8.346
Patrickios 4.724
IPC_peptide 8.492
IPC2_peptide 7.307
IPC2.peptide.svr19 7.564
Protein with the highest isoelectric point:
>tr|A0A1L3KFI2|A0A1L3KFI2_9VIRU Capsid protein OS=Beihai sphaeromadae virus 3 OX=1922709 PE=4 SV=1
MM1 pKa = 7.55 EE2 pKa = 5.39 SNVAITVRR10 pKa = 11.84 PNDD13 pKa = 3.24 IGHH16 pKa = 6.51 FFSLRR21 pKa = 11.84 SVASNSLKK29 pKa = 10.59 IGGICLLGVASYY41 pKa = 9.91 KK42 pKa = 10.18 ICTSGWLEE50 pKa = 3.91 KK51 pKa = 10.63 CVEE54 pKa = 4.29 DD55 pKa = 3.86 LRR57 pKa = 11.84 HH58 pKa = 6.61 RR59 pKa = 11.84 IISRR63 pKa = 11.84 NKK65 pKa = 8.95 TVINDD70 pKa = 3.61 AGLVRR75 pKa = 11.84 ATVQKK80 pKa = 10.85 KK81 pKa = 8.47 MEE83 pKa = 4.31 LVSTRR88 pKa = 11.84 VRR90 pKa = 11.84 KK91 pKa = 9.19 GHH93 pKa = 5.17 SHH95 pKa = 4.88 STAAAEE101 pKa = 4.14 RR102 pKa = 11.84 NSATEE107 pKa = 3.72 TMIEE111 pKa = 4.17 VVTANGFEE119 pKa = 4.64 PYY121 pKa = 10.12 VISPSPRR128 pKa = 11.84 EE129 pKa = 3.76 SDD131 pKa = 3.13 LDD133 pKa = 4.26 GIRR136 pKa = 11.84 HH137 pKa = 5.23 FHH139 pKa = 6.36 SLADD143 pKa = 3.6 LRR145 pKa = 11.84 QDD147 pKa = 3.55 YY148 pKa = 11.16 RR149 pKa = 11.84 NDD151 pKa = 3.79 PLTDD155 pKa = 2.94 RR156 pKa = 11.84 HH157 pKa = 6.92 IIVMTDD163 pKa = 1.97 VDD165 pKa = 4.86 YY166 pKa = 11.07 YY167 pKa = 11.9 VNMHH171 pKa = 6.71 SIISLGRR178 pKa = 11.84 PILLYY183 pKa = 9.38 TFQPRR188 pKa = 11.84 VVSGPVIDD196 pKa = 4.64 GFFTITEE203 pKa = 4.29 NIIHH207 pKa = 5.74 YY208 pKa = 9.53 RR209 pKa = 11.84 VNGGKK214 pKa = 9.43 DD215 pKa = 3.32 VKK217 pKa = 10.98 HH218 pKa = 5.69 PTWNYY223 pKa = 8.83 NQDD226 pKa = 3.34 TVFVRR231 pKa = 11.84 DD232 pKa = 3.48 PVTTFWATLRR242 pKa = 11.84 QTLFDD247 pKa = 3.45 VTGISWISAKK257 pKa = 7.19 MHH259 pKa = 6.15 RR260 pKa = 11.84 AFGIGPCGQQLTICTIDD277 pKa = 3.47 QFEE280 pKa = 4.59 LSPNRR285 pKa = 11.84 NIVSIVPFARR295 pKa = 11.84 CRR297 pKa = 11.84 DD298 pKa = 3.71 NLLTATEE305 pKa = 4.38 YY306 pKa = 9.66 GTEE309 pKa = 4.22 LSRR312 pKa = 11.84 TTYY315 pKa = 7.59 QQKK318 pKa = 10.66 SGVPVMNAITYY329 pKa = 10.16 IGDD332 pKa = 3.76 GDD334 pKa = 4.12 PLISLGEE341 pKa = 4.11 EE342 pKa = 4.46 GNCASVQLPLKK353 pKa = 10.26 DD354 pKa = 4.1 LEE356 pKa = 4.51 SLRR359 pKa = 11.84 TAYY362 pKa = 10.36 HH363 pKa = 6.72 LSKK366 pKa = 10.02 TNNLSDD372 pKa = 3.38 TVRR375 pKa = 11.84 RR376 pKa = 11.84 SKK378 pKa = 11.2 RR379 pKa = 11.84 NDD381 pKa = 2.82 KK382 pKa = 9.98 EE383 pKa = 3.43 AAIIHH388 pKa = 6.32 RR389 pKa = 11.84 FLVSEE394 pKa = 4.53 SEE396 pKa = 4.01 LHH398 pKa = 5.73 PVEE401 pKa = 4.09 VHH403 pKa = 6.96 KK404 pKa = 10.66 PGQLARR410 pKa = 11.84 HH411 pKa = 4.95 YY412 pKa = 10.69 QSVEE416 pKa = 3.73 KK417 pKa = 10.99 DD418 pKa = 3.07 HH419 pKa = 7.3 DD420 pKa = 4.7 LDD422 pKa = 3.58 PTEE425 pKa = 3.92 QGKK428 pKa = 10.13 EE429 pKa = 3.91 YY430 pKa = 10.63 ARR432 pKa = 11.84 EE433 pKa = 3.96 YY434 pKa = 11.32 APGPLTQTAVFPSEE448 pKa = 4.37 SISNEE453 pKa = 3.28 RR454 pKa = 11.84 ATIEE458 pKa = 3.93 GRR460 pKa = 11.84 INLPQAKK467 pKa = 9.38 AKK469 pKa = 10.37 AKK471 pKa = 10.23 EE472 pKa = 4.33 SVTPRR477 pKa = 11.84 MRR479 pKa = 11.84 RR480 pKa = 11.84 IARR483 pKa = 11.84 DD484 pKa = 3.64 FVKK487 pKa = 10.66 HH488 pKa = 5.86 LVPEE492 pKa = 4.22 VGIGHH497 pKa = 7.88 PYY499 pKa = 9.64 SVSHH503 pKa = 7.43 VEE505 pKa = 4.11 EE506 pKa = 4.32 QQQKK510 pKa = 8.97 PLQRR514 pKa = 11.84 ARR516 pKa = 11.84 NDD518 pKa = 3.01 ANRR521 pKa = 11.84 FHH523 pKa = 7.63 DD524 pKa = 4.72 AFDD527 pKa = 4.49 MITKK531 pKa = 10.07 AFQKK535 pKa = 10.51 KK536 pKa = 7.59 EE537 pKa = 3.78 AYY539 pKa = 8.25 NAPNHH544 pKa = 6.13 PRR546 pKa = 11.84 NISTVPHH553 pKa = 5.58 GQNVKK558 pKa = 10.69 LSGFTYY564 pKa = 10.53 AFKK567 pKa = 10.51 EE568 pKa = 4.13 SHH570 pKa = 6.71 LKK572 pKa = 8.42 HH573 pKa = 6.02 QEE575 pKa = 3.02 WYY577 pKa = 8.86 MPCNTPSKK585 pKa = 9.84 IASVVQKK592 pKa = 10.39 LAAEE596 pKa = 4.29 SEE598 pKa = 4.45 QLVEE602 pKa = 3.78 TDD604 pKa = 3.24 YY605 pKa = 11.78 SRR607 pKa = 11.84 FDD609 pKa = 3.3 GTFLRR614 pKa = 11.84 FMRR617 pKa = 11.84 EE618 pKa = 3.71 QVEE621 pKa = 4.22 FASYY625 pKa = 10.12 KK626 pKa = 9.32 RR627 pKa = 11.84 WVHH630 pKa = 6.25 KK631 pKa = 10.4 DD632 pKa = 3.27 HH633 pKa = 6.69 IQEE636 pKa = 4.19 LTDD639 pKa = 3.94 LLANEE644 pKa = 4.42 VDD646 pKa = 3.94 SKK648 pKa = 11.68 AVTRR652 pKa = 11.84 KK653 pKa = 9.35 GLKK656 pKa = 8.96 YY657 pKa = 10.61 QPDD660 pKa = 4.03 CTRR663 pKa = 11.84 LSGSALTTDD672 pKa = 3.96 GNSIANAFVSYY683 pKa = 10.39 AANRR687 pKa = 11.84 LDD689 pKa = 3.78 GMNDD693 pKa = 2.72 VEE695 pKa = 3.87 AWQRR699 pKa = 11.84 IGIVYY704 pKa = 10.33 GDD706 pKa = 3.88 DD707 pKa = 3.55 GLRR710 pKa = 11.84 DD711 pKa = 3.74 GTVSDD716 pKa = 4.64 DD717 pKa = 3.84 RR718 pKa = 11.84 LMSTASSLGFEE729 pKa = 4.26 LKK731 pKa = 10.26 IINRR735 pKa = 11.84 ATRR738 pKa = 11.84 GQPVSFLSRR747 pKa = 11.84 IYY749 pKa = 10.77 ADD751 pKa = 3.62 PWSSPASVQTPSRR764 pKa = 11.84 TLLKK768 pKa = 10.35 IHH770 pKa = 6.09 TTCDD774 pKa = 3.21 TQSDD778 pKa = 3.88 IEE780 pKa = 4.26 EE781 pKa = 4.27 VGWAKK786 pKa = 8.65 TQAYY790 pKa = 9.84 LVTDD794 pKa = 4.46 CLTPFISNWCKK805 pKa = 10.29 AYY807 pKa = 10.46 QRR809 pKa = 11.84 NCTAKK814 pKa = 10.24 VINYY818 pKa = 9.67 KK819 pKa = 10.5 DD820 pKa = 3.79 FNDD823 pKa = 3.24 IPFWVRR829 pKa = 11.84 DD830 pKa = 3.19 EE831 pKa = 5.68 DD832 pKa = 4.0 ALQNSWPQDD841 pKa = 3.54 NSEE844 pKa = 3.49 VWYY847 pKa = 9.98 DD848 pKa = 4.27 IVAQDD853 pKa = 3.96 LGVSVGEE860 pKa = 3.96 LHH862 pKa = 7.11 RR863 pKa = 11.84 HH864 pKa = 5.43 LKK866 pKa = 10.77 LLDD869 pKa = 3.93 SYY871 pKa = 11.52 NGPIKK876 pKa = 10.21 DD877 pKa = 4.18 LPRR880 pKa = 11.84 LATALKK886 pKa = 7.6 TQPKK890 pKa = 9.56 LAVAMDD896 pKa = 4.14 GEE898 pKa = 4.59 IHH900 pKa = 7.47 AGPTEE905 pKa = 4.09 QNGQNPTSDD914 pKa = 3.31 QPASDD919 pKa = 4.26 GATKK923 pKa = 10.55 ALPAVGGGKK932 pKa = 8.68 NQSGRR937 pKa = 11.84 TVSVRR942 pKa = 11.84 HH943 pKa = 5.43 QRR945 pKa = 11.84 NAHH948 pKa = 6.24 LRR950 pKa = 11.84 DD951 pKa = 3.5 QRR953 pKa = 11.84 PGSNNKK959 pKa = 8.18 TAGPGHH965 pKa = 7.1 KK966 pKa = 9.52 ISTPNGGTSQSSARR980 pKa = 11.84 TNCAAGRR987 pKa = 11.84 RR988 pKa = 11.84 SAPQVGRR995 pKa = 11.84 KK996 pKa = 6.65 KK997 pKa = 8.76 TRR999 pKa = 11.84 KK1000 pKa = 9.28 AA1001 pKa = 3.15
Molecular weight: 111.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.614
IPC2_protein 7.936
IPC_protein 7.761
Toseland 7.658
ProMoST 8.346
Dawson 8.551
Bjellqvist 8.785
Wikipedia 8.521
Rodwell 8.609
Grimsley 7.746
Solomon 8.609
Lehninger 8.609
Nozaki 8.931
DTASelect 8.565
Thurlkill 8.639
EMBOSS 8.741
Sillero 8.916
Patrickios 4.584
IPC_peptide 8.609
IPC2_peptide 7.614
IPC2.peptide.svr19 7.794
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1402
401
1001
701.0
77.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.131 ± 0.814
1.07 ± 0.419
5.777 ± 0.403
4.85 ± 0.184
3.352 ± 0.326
6.419 ± 0.287
2.71 ± 0.874
5.421 ± 0.221
4.494 ± 1.021
7.204 ± 0.142
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.498 ± 0.126
5.777 ± 0.87
5.991 ± 1.142
4.208 ± 0.493
6.99 ± 0.123
8.345 ± 0.577
6.919 ± 0.222
6.776 ± 0.276
1.284 ± 0.019
2.782 ± 0.274
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here