Pseudooceanicola lipolyticus
Average proteome isoelectric point is 6.18
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5156 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2M8IVR8|A0A2M8IVR8_9RHOB Corrinoid adenosyltransferase OS=Pseudooceanicola lipolyticus OX=2029104 GN=cobO PE=3 SV=1
MM1 pKa = 7.84 KK2 pKa = 10.34 YY3 pKa = 8.85 LTFAAGVFGASAALAQTPEE22 pKa = 4.31 LTVYY26 pKa = 10.09 TYY28 pKa = 11.48 DD29 pKa = 3.62 SFVAEE34 pKa = 5.0 WGPGPQIEE42 pKa = 4.53 TAFEE46 pKa = 4.05 AVCNCDD52 pKa = 3.27 LTLVAAGDD60 pKa = 4.04 GAALLARR67 pKa = 11.84 LQLEE71 pKa = 4.35 GDD73 pKa = 3.32 RR74 pKa = 11.84 TEE76 pKa = 4.96 ADD78 pKa = 3.47 VVLGLDD84 pKa = 3.72 TNLVPAARR92 pKa = 11.84 EE93 pKa = 3.87 TGLFAPHH100 pKa = 6.6 GADD103 pKa = 3.09 ATLDD107 pKa = 4.42 LPITWDD113 pKa = 3.99 DD114 pKa = 3.91 DD115 pKa = 3.86 TFLPYY120 pKa = 10.65 DD121 pKa = 3.12 WGYY124 pKa = 10.36 FAFIHH129 pKa = 5.55 NTDD132 pKa = 4.14 LANVPQDD139 pKa = 3.86 FLEE142 pKa = 4.53 LAASDD147 pKa = 4.19 LSILIQDD154 pKa = 4.48 PRR156 pKa = 11.84 SSTPGLGLLMWVKK169 pKa = 10.34 AAYY172 pKa = 9.95 GDD174 pKa = 3.66 EE175 pKa = 4.63 APAVWAALADD185 pKa = 4.22 NIVTVTKK192 pKa = 10.12 GWSEE196 pKa = 4.19 AYY198 pKa = 10.37 GLFLDD203 pKa = 5.47 GEE205 pKa = 4.66 ADD207 pKa = 3.4 MVLSYY212 pKa = 7.66 TTSPAYY218 pKa = 10.17 HH219 pKa = 7.21 IIAEE223 pKa = 4.6 DD224 pKa = 3.82 DD225 pKa = 3.63 TSKK228 pKa = 11.22 DD229 pKa = 2.86 AAAFDD234 pKa = 4.04 EE235 pKa = 4.31 GHH237 pKa = 6.05 YY238 pKa = 10.6 LQIEE242 pKa = 4.46 LAAPLAGSDD251 pKa = 3.84 QPEE254 pKa = 4.04 LAAQFMQFITTDD266 pKa = 3.28 AFQSVIPTTNWMYY279 pKa = 9.9 PAVMPADD286 pKa = 4.04 GLPAEE291 pKa = 4.94 FDD293 pKa = 3.65 TLVEE297 pKa = 4.17 PRR299 pKa = 11.84 NALLIPEE306 pKa = 4.58 AEE308 pKa = 4.32 VTEE311 pKa = 4.53 LRR313 pKa = 11.84 AQALAEE319 pKa = 3.99 WLDD322 pKa = 3.67 ALSQQ326 pKa = 3.47
Molecular weight: 35.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.643
IPC_protein 3.656
Toseland 3.439
ProMoST 3.821
Dawson 3.643
Bjellqvist 3.808
Wikipedia 3.579
Rodwell 3.478
Grimsley 3.338
Solomon 3.643
Lehninger 3.592
Nozaki 3.757
DTASelect 3.999
Thurlkill 3.49
EMBOSS 3.592
Sillero 3.783
Patrickios 0.871
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.7
Protein with the highest isoelectric point:
>tr|A0A2M8IYF9|A0A2M8IYF9_9RHOB sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC OS=Pseudooceanicola lipolyticus OX=2029104 GN=CVM52_16705 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.43 EE41 pKa = 3.72 LSAA44 pKa = 5.03
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.423
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.092
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5156
0
5156
1548121
22
3102
300.3
32.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.644 ± 0.042
0.905 ± 0.011
5.991 ± 0.034
5.851 ± 0.035
3.736 ± 0.022
8.798 ± 0.037
2.016 ± 0.016
5.058 ± 0.022
2.83 ± 0.025
10.295 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.679 ± 0.018
2.482 ± 0.018
5.191 ± 0.025
3.294 ± 0.02
7.003 ± 0.035
5.088 ± 0.022
5.356 ± 0.026
7.193 ± 0.026
1.35 ± 0.013
2.24 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here