Sewage-associated circular DNA virus-4

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075J462|A0A075J462_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-4 OX=1519393 PE=3 SV=1
MM1 pKa = 7.48ARR3 pKa = 11.84FHH5 pKa = 7.0AKK7 pKa = 8.6TVFLTYY13 pKa = 9.06PQCPVNKK20 pKa = 9.38EE21 pKa = 4.19DD22 pKa = 4.8LLAWLQTLRR31 pKa = 11.84NPIYY35 pKa = 10.11TIVSHH40 pKa = 6.13EE41 pKa = 4.03LHH43 pKa = 6.97EE44 pKa = 5.46DD45 pKa = 4.13GSPHH49 pKa = 5.93LHH51 pKa = 7.02AVVCWQNKK59 pKa = 9.12LDD61 pKa = 4.01IRR63 pKa = 11.84DD64 pKa = 3.73TTFFNYY70 pKa = 9.84EE71 pKa = 3.93NYY73 pKa = 10.13HH74 pKa = 6.41PNIQSARR81 pKa = 11.84SIKK84 pKa = 10.41KK85 pKa = 9.74VIAYY89 pKa = 6.93TKK91 pKa = 10.44KK92 pKa = 10.6DD93 pKa = 3.06GDD95 pKa = 4.02FIEE98 pKa = 6.23AGDD101 pKa = 4.07SPIVDD106 pKa = 4.08TANTWSTAMDD116 pKa = 3.53TATTIKK122 pKa = 10.8EE123 pKa = 3.97FMDD126 pKa = 3.68TVKK129 pKa = 10.65EE130 pKa = 4.32SNPRR134 pKa = 11.84DD135 pKa = 3.6YY136 pKa = 10.92ILQHH140 pKa = 5.82EE141 pKa = 4.27RR142 pKa = 11.84LEE144 pKa = 4.32YY145 pKa = 10.19FAAKK149 pKa = 9.9HH150 pKa = 5.24FKK152 pKa = 10.46KK153 pKa = 9.96DD154 pKa = 3.39TEE156 pKa = 4.44VYY158 pKa = 10.08KK159 pKa = 11.0PNFEE163 pKa = 4.25EE164 pKa = 5.58FILPHH169 pKa = 6.32EE170 pKa = 4.46VEE172 pKa = 3.63AWLNEE177 pKa = 3.84EE178 pKa = 4.21FNSDD182 pKa = 3.62KK183 pKa = 10.85LRR185 pKa = 11.84KK186 pKa = 9.35KK187 pKa = 10.56SLILIGPSKK196 pKa = 10.5LGKK199 pKa = 8.13TEE201 pKa = 3.82WARR204 pKa = 11.84SLGPHH209 pKa = 6.44MYY211 pKa = 10.19FNHH214 pKa = 6.89LANFKK219 pKa = 10.76DD220 pKa = 3.77DD221 pKa = 3.89WDD223 pKa = 5.2DD224 pKa = 3.35EE225 pKa = 4.4AKK227 pKa = 11.1YY228 pKa = 10.58IVFDD232 pKa = 4.84DD233 pKa = 4.08FDD235 pKa = 6.0FDD237 pKa = 5.53FIPNKK242 pKa = 10.23KK243 pKa = 10.25GFFGGQQHH251 pKa = 5.61FQITGKK257 pKa = 9.21YY258 pKa = 7.38MRR260 pKa = 11.84VKK262 pKa = 9.64SVRR265 pKa = 11.84WGKK268 pKa = 10.21CCIYY272 pKa = 9.98CANVTPNIKK281 pKa = 10.62DD282 pKa = 3.41IDD284 pKa = 4.02KK285 pKa = 10.8DD286 pKa = 3.61WYY288 pKa = 11.06NEE290 pKa = 3.6NCVFIDD296 pKa = 3.75VVNKK300 pKa = 9.45FYY302 pKa = 11.39

Molecular weight: 35.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075J462|A0A075J462_9VIRU Replication-associated protein OS=Sewage-associated circular DNA virus-4 OX=1519393 PE=3 SV=1
MM1 pKa = 7.69PKK3 pKa = 8.85YY4 pKa = 10.5AKK6 pKa = 9.73RR7 pKa = 11.84RR8 pKa = 11.84KK9 pKa = 7.23YY10 pKa = 8.7TNRR13 pKa = 11.84AAKK16 pKa = 9.94AFKK19 pKa = 10.43KK20 pKa = 10.23SVPKK24 pKa = 10.46TSTKK28 pKa = 8.57TIRR31 pKa = 11.84RR32 pKa = 11.84IAVKK36 pKa = 10.14AIKK39 pKa = 10.2SQAEE43 pKa = 4.11TKK45 pKa = 10.18NYY47 pKa = 8.55YY48 pKa = 8.03YY49 pKa = 10.64HH50 pKa = 7.45WLVQNQSTAFPYY62 pKa = 10.51AHH64 pKa = 6.54NAFYY68 pKa = 10.44EE69 pKa = 4.28INQGVGDD76 pKa = 3.89NQRR79 pKa = 11.84IGDD82 pKa = 3.88KK83 pKa = 10.74VYY85 pKa = 11.02VSRR88 pKa = 11.84LTYY91 pKa = 10.84KK92 pKa = 10.73LGFTIDD98 pKa = 3.75SNFFAPFGVRR108 pKa = 11.84GDD110 pKa = 3.33IYY112 pKa = 11.05VRR114 pKa = 11.84CLLVQNKK121 pKa = 8.63ARR123 pKa = 11.84QNNGITGFLPTWGGSVLMKK142 pKa = 10.35SGYY145 pKa = 8.16FQNGIVDD152 pKa = 3.99KK153 pKa = 10.94EE154 pKa = 4.21QFTVLRR160 pKa = 11.84DD161 pKa = 3.81RR162 pKa = 11.84NFHH165 pKa = 6.74LSPGNAINGKK175 pKa = 4.98QTYY178 pKa = 8.66YY179 pKa = 11.02RR180 pKa = 11.84NISVRR185 pKa = 11.84WAKK188 pKa = 10.49NIQYY192 pKa = 10.87DD193 pKa = 3.9SDD195 pKa = 3.43NGGYY199 pKa = 8.97MKK201 pKa = 10.74FKK203 pKa = 9.47NTYY206 pKa = 9.8LIYY209 pKa = 10.31QIYY212 pKa = 10.18SPDD215 pKa = 3.58GYY217 pKa = 7.85TTGGFTTNIVHH228 pKa = 7.25NIQFKK233 pKa = 10.89DD234 pKa = 3.43LL235 pKa = 3.76

Molecular weight: 27.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

537

235

302

268.5

31.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.773 ± 0.116

1.304 ± 0.55

6.518 ± 1.418

4.097 ± 1.768

6.704 ± 0.468

5.587 ± 1.566

2.98 ± 0.801

6.704 ± 0.066

9.125 ± 0.118

5.4 ± 0.451

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.49 ± 0.134

7.263 ± 0.783

3.724 ± 0.468

3.911 ± 1.017

4.469 ± 0.933

4.469 ± 0.399

6.518 ± 0.449

5.773 ± 0.116

2.048 ± 0.484

6.145 ± 1.216
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski