Streptococcus satellite phage Javan62

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 7.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZSN4|A0A4D5ZSN4_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan62 OX=2558787 GN=JavanS62_0004 PE=4 SV=1
MM1 pKa = 7.77ADD3 pKa = 3.37KK4 pKa = 11.27KK5 pKa = 11.09EE6 pKa = 3.65FDD8 pKa = 4.13LANEE12 pKa = 3.91RR13 pKa = 11.84AKK15 pKa = 11.2NFGIWLEE22 pKa = 4.04EE23 pKa = 4.33AYY25 pKa = 8.85QTMLDD30 pKa = 4.09FSLEE34 pKa = 4.14NKK36 pKa = 9.55FDD38 pKa = 3.95CYY40 pKa = 10.92SIEE43 pKa = 4.08EE44 pKa = 4.21RR45 pKa = 11.84NQLEE49 pKa = 4.34RR50 pKa = 11.84VLEE53 pKa = 4.07TLMDD57 pKa = 4.18FCDD60 pKa = 3.07MWEE63 pKa = 4.02RR64 pKa = 11.84GQIILASKK72 pKa = 9.62EE73 pKa = 3.85RR74 pKa = 11.84EE75 pKa = 4.13AIEE78 pKa = 3.87

Molecular weight: 9.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZXH5|A0A4D5ZXH5_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan62 OX=2558787 GN=JavanS62_0018 PE=4 SV=1
MM1 pKa = 7.41ARR3 pKa = 11.84YY4 pKa = 9.3NIHH7 pKa = 6.8PAQGGGKK14 pKa = 8.08YY15 pKa = 10.13HH16 pKa = 7.47DD17 pKa = 3.42MKK19 pKa = 10.41IYY21 pKa = 10.59KK22 pKa = 9.83DD23 pKa = 3.91RR24 pKa = 11.84QPKK27 pKa = 10.18ALTHH31 pKa = 6.15EE32 pKa = 4.16EE33 pKa = 3.92QKK35 pKa = 10.63RR36 pKa = 11.84LKK38 pKa = 10.53RR39 pKa = 11.84LTKK42 pKa = 9.88KK43 pKa = 9.78RR44 pKa = 11.84RR45 pKa = 11.84KK46 pKa = 9.73GRR48 pKa = 3.51

Molecular weight: 5.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20

0

20

3076

42

443

153.8

17.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.787 ± 0.504

0.91 ± 0.197

5.527 ± 0.42

6.762 ± 0.582

4.811 ± 0.403

5.267 ± 0.38

2.048 ± 0.297

6.404 ± 0.343

8.713 ± 0.479

10.306 ± 0.515

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.503 ± 0.263

5.234 ± 0.674

3.706 ± 0.446

3.869 ± 0.432

5.689 ± 0.453

5.689 ± 0.394

5.787 ± 0.35

5.202 ± 0.662

0.845 ± 0.153

4.941 ± 0.415
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski