Branchiostoma floridae (Florida lancelet) (Amphioxus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Cephalochordata; Leptocardii; Amphioxiformes; Branchiostomidae; Branchiostoma

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 28544 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|C3ZYJ5|C3ZYJ5_BRAFL Uncharacterized protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_108653 PE=4 SV=1
MM1 pKa = 7.3ATRR4 pKa = 11.84GVEE7 pKa = 3.71QAGVLRR13 pKa = 11.84CSLLVVPGATCRR25 pKa = 11.84AAMRR29 pKa = 11.84GSFSAEE35 pKa = 3.68EE36 pKa = 3.92FSSRR40 pKa = 11.84EE41 pKa = 4.02MVRR44 pKa = 11.84SATLLVAFALLASTAHH60 pKa = 5.74GQFDD64 pKa = 4.66FGNIDD69 pKa = 3.93LDD71 pKa = 3.98GTGDD75 pKa = 3.6PFIILTRR82 pKa = 11.84PPTQAPVANPVVVQLLGQVSDD103 pKa = 5.64LIRR106 pKa = 11.84QSGDD110 pKa = 2.96PDD112 pKa = 3.55GAFFAEE118 pKa = 4.4YY119 pKa = 10.39LVGEE123 pKa = 4.74LATAGITVDD132 pKa = 4.61GLTQGIITSWSNALATEE149 pKa = 4.13EE150 pKa = 4.03NNYY153 pKa = 10.38EE154 pKa = 4.09SLALQFEE161 pKa = 5.06SVGTQLGFNAAQVAAKK177 pKa = 10.22AVADD181 pKa = 3.76YY182 pKa = 11.45NEE184 pKa = 4.32WLNVTAILRR193 pKa = 11.84ALSARR198 pKa = 11.84LQGISPDD205 pKa = 4.0NIGGVAWQISTALSSYY221 pKa = 10.65GYY223 pKa = 9.54PSDD226 pKa = 3.54NASIEE231 pKa = 4.69GIYY234 pKa = 10.31NWTVALVAAGNNTDD248 pKa = 3.37GVAMDD253 pKa = 4.37FYY255 pKa = 11.52GVAAAVGVSPGSLAQDD271 pKa = 3.99LQQLLTAALDD281 pKa = 3.79EE282 pKa = 4.87GEE284 pKa = 4.82LVYY287 pKa = 10.2FYY289 pKa = 11.26LMEE292 pKa = 4.42VKK294 pKa = 10.24QVMSNFNTSYY304 pKa = 10.41IRR306 pKa = 11.84QFSILIAQEE315 pKa = 4.29LNTLGFPLTPEE326 pKa = 3.9NSEE329 pKa = 4.36TIYY332 pKa = 9.85MWTLGLYY339 pKa = 10.16NNNDD343 pKa = 3.09GDD345 pKa = 3.47IGVYY349 pKa = 9.97INQFYY354 pKa = 10.22EE355 pKa = 4.13VAASVNKK362 pKa = 10.17SASEE366 pKa = 3.59LAKK369 pKa = 10.37IVWEE373 pKa = 4.56DD374 pKa = 3.68LTSQLQGGAAVTAHH388 pKa = 7.08LIEE391 pKa = 4.18VRR393 pKa = 11.84NLLRR397 pKa = 11.84LVEE400 pKa = 4.54FSYY403 pKa = 10.91IRR405 pKa = 11.84GLSEE409 pKa = 4.38FIAYY413 pKa = 9.78QLVPLGYY420 pKa = 9.17PVQNDD425 pKa = 3.88TFEE428 pKa = 4.79VMYY431 pKa = 8.79TWTVGMFGNDD441 pKa = 4.42DD442 pKa = 3.85ITPLIQQFYY451 pKa = 11.0DD452 pKa = 3.39VASLVNVSAADD463 pKa = 3.62LALGLKK469 pKa = 10.16DD470 pKa = 4.77ALTAQLTGGMSVVTDD485 pKa = 4.62LIEE488 pKa = 4.23VKK490 pKa = 10.49RR491 pKa = 11.84LLKK494 pKa = 10.07TSEE497 pKa = 3.76FQYY500 pKa = 10.9IDD502 pKa = 4.36DD503 pKa = 4.94LSLLITGALVPYY515 pKa = 9.83GLEE518 pKa = 4.05YY519 pKa = 11.06NNEE522 pKa = 4.43SIASIYY528 pKa = 10.62NWTAGLYY535 pKa = 10.52LSDD538 pKa = 6.08DD539 pKa = 3.26IQPYY543 pKa = 9.43VEE545 pKa = 4.09QFYY548 pKa = 10.85QVAYY552 pKa = 9.08MLNTSAPDD560 pKa = 3.44LAAGLRR566 pKa = 11.84QLLTDD571 pKa = 3.76HH572 pKa = 6.51FAEE575 pKa = 4.48VEE577 pKa = 4.27QVVMDD582 pKa = 4.55LIEE585 pKa = 4.32VKK587 pKa = 10.57RR588 pKa = 11.84LLNTVEE594 pKa = 4.23FKK596 pKa = 11.21YY597 pKa = 11.13VFGLSQLITGALGQYY612 pKa = 9.48GLEE615 pKa = 4.24YY616 pKa = 10.96NNEE619 pKa = 3.94TEE621 pKa = 4.04AFVYY625 pKa = 10.02QWTADD630 pKa = 3.64LYY632 pKa = 10.9NAQDD636 pKa = 3.55DD637 pKa = 4.34FGPQVEE643 pKa = 4.3QFYY646 pKa = 10.83QVAYY650 pKa = 8.38MLNVSVSYY658 pKa = 10.74LSNGLKK664 pKa = 10.04QLLTDD669 pKa = 4.24HH670 pKa = 6.52FAEE673 pKa = 4.54VNQVVTDD680 pKa = 4.0LLEE683 pKa = 4.26VKK685 pKa = 10.33RR686 pKa = 11.84LLGTVEE692 pKa = 3.8FQYY695 pKa = 11.34VYY697 pKa = 10.96DD698 pKa = 4.52LSLLITANLMPYY710 pKa = 9.48GLEE713 pKa = 4.05YY714 pKa = 10.93NNEE717 pKa = 4.38SIAMVYY723 pKa = 10.11GWTAGLYY730 pKa = 8.75FAQNDD735 pKa = 3.24IGPQVEE741 pKa = 4.11QFYY744 pKa = 11.07QLAYY748 pKa = 9.21MLNTSVVDD756 pKa = 3.99LAAGLKK762 pKa = 10.21QILTEE767 pKa = 4.67HH768 pKa = 6.63FDD770 pKa = 3.43KK771 pKa = 11.51VNAVVDD777 pKa = 3.77NLLQVQTLLLTLDD790 pKa = 3.65EE791 pKa = 5.0AFLPGLGQYY800 pKa = 7.78VTGQLATLGFPYY812 pKa = 10.49NDD814 pKa = 3.24EE815 pKa = 4.11TVAQLTQWLTDD826 pKa = 3.11IRR828 pKa = 11.84NGLDD832 pKa = 3.07FGAYY836 pKa = 8.39VRR838 pKa = 11.84QFYY841 pKa = 10.15EE842 pKa = 3.9VAAVVGVPEE851 pKa = 4.39VEE853 pKa = 4.23LSGNLLAGLRR863 pKa = 11.84EE864 pKa = 4.07QIALGEE870 pKa = 3.97AVYY873 pKa = 11.32YY874 pKa = 10.28DD875 pKa = 3.42IFEE878 pKa = 4.89ANQVLPQLSDD888 pKa = 2.95MDD890 pKa = 3.77VANTVNFIINFITDD904 pKa = 3.53RR905 pKa = 11.84GVMVPMDD912 pKa = 4.34LEE914 pKa = 3.87QGIRR918 pKa = 11.84NWTIALAQPSADD930 pKa = 3.72LQPIIQEE937 pKa = 4.17LYY939 pKa = 10.86GVAEE943 pKa = 5.31LINIPVRR950 pKa = 11.84DD951 pKa = 3.79MSGALVDD958 pKa = 3.91HH959 pKa = 6.72LRR961 pKa = 11.84KK962 pKa = 9.2EE963 pKa = 4.57HH964 pKa = 6.63YY965 pKa = 10.17SVPDD969 pKa = 3.8YY970 pKa = 11.29IEE972 pKa = 4.06VVALILQDD980 pKa = 3.44INVAYY985 pKa = 10.05LKK987 pKa = 10.46NVSVEE992 pKa = 3.55ITQILTGLGIPVPAPEE1008 pKa = 4.06LQMTLFEE1015 pKa = 4.33WVEE1018 pKa = 4.22NIALKK1023 pKa = 10.82PEE1025 pKa = 4.47DD1026 pKa = 4.0LPLYY1030 pKa = 9.41IDD1032 pKa = 3.45QFRR1035 pKa = 11.84EE1036 pKa = 3.7VAALVNVNIDD1046 pKa = 3.4EE1047 pKa = 4.37LAYY1050 pKa = 10.44GLLSEE1055 pKa = 4.85VEE1057 pKa = 4.33TLAEE1061 pKa = 4.15AFPDD1065 pKa = 4.06GFSPSVQNILDD1076 pKa = 3.97NLPEE1080 pKa = 4.08LMQLFSTYY1088 pKa = 10.17FPDD1091 pKa = 5.96FYY1093 pKa = 11.55ANVNNQVQQFGMAAWANVSANLTNYY1118 pKa = 9.46FNNAPPEE1125 pKa = 3.85QAMMFEE1131 pKa = 4.38NKK1133 pKa = 10.08LPEE1136 pKa = 3.94IAALLGIDD1144 pKa = 3.42IPANVLQGDD1153 pKa = 4.08ANAIIQYY1160 pKa = 9.48IQSQLPDD1167 pKa = 3.47MYY1169 pKa = 9.98ILSSAIQQALEE1180 pKa = 4.08AMTQQFPAQLVGEE1193 pKa = 4.22LLEE1196 pKa = 4.67VIFGVDD1202 pKa = 3.02IPDD1205 pKa = 4.56DD1206 pKa = 3.86FSFDD1210 pKa = 3.2PTYY1213 pKa = 10.43IVQQIPVFLGSLGVQVPSNYY1233 pKa = 9.77YY1234 pKa = 10.24DD1235 pKa = 2.82IVMAFASFEE1244 pKa = 3.99QTPEE1248 pKa = 3.26WFANNGATIVSPFGISLPDD1267 pKa = 3.7DD1268 pKa = 3.98FQPTEE1273 pKa = 4.12EE1274 pKa = 4.18YY1275 pKa = 10.79VAANLDD1281 pKa = 4.31KK1282 pKa = 10.69IIQTIANQAPQIVGVLYY1299 pKa = 8.77NTTQQIFMQVQQNIAVNSGGDD1320 pKa = 3.06VGMFIQQIIQKK1331 pKa = 9.76VLDD1334 pKa = 4.47LLPGASDD1341 pKa = 3.52YY1342 pKa = 11.48FGFYY1346 pKa = 10.48LPEE1349 pKa = 4.85DD1350 pKa = 3.81YY1351 pKa = 10.51RR1352 pKa = 11.84PEE1354 pKa = 5.1LIYY1357 pKa = 10.36FAEE1360 pKa = 4.04WGINEE1365 pKa = 4.91LFNLIPTKK1373 pKa = 10.87EE1374 pKa = 4.45EE1375 pKa = 3.87FVQMAVQPIAATLAPNDD1392 pKa = 3.31TWIAEE1397 pKa = 4.12LLEE1400 pKa = 4.52DD1401 pKa = 4.2QFSLIYY1407 pKa = 9.75DD1408 pKa = 4.14LNMVYY1413 pKa = 9.75IEE1415 pKa = 4.73HH1416 pKa = 6.58MPDD1419 pKa = 2.84ALKK1422 pKa = 10.37WIGRR1426 pKa = 11.84WLGFEE1431 pKa = 4.67LPDD1434 pKa = 4.38DD1435 pKa = 4.55FKK1437 pKa = 11.72LEE1439 pKa = 4.2PEE1441 pKa = 4.03WFIAQLPMVQAYY1453 pKa = 9.51VEE1455 pKa = 4.19QNAAMWFDD1463 pKa = 3.59QATMMFAEE1471 pKa = 4.65WSEE1474 pKa = 3.96QANMWISGFMTDD1486 pKa = 3.22SQGWNDD1492 pKa = 4.81MVNAWINDD1500 pKa = 3.67LPQTVAGLFNLDD1512 pKa = 3.84TLFMYY1517 pKa = 10.61LDD1519 pKa = 4.1PANAPEE1525 pKa = 4.52WMTMTPPPIPFDD1537 pKa = 3.62AFLTDD1542 pKa = 3.63GCVHH1546 pKa = 7.37AIAFILDD1553 pKa = 3.05VDD1555 pKa = 3.57IPEE1558 pKa = 4.77DD1559 pKa = 3.79YY1560 pKa = 10.87KK1561 pKa = 11.57VDD1563 pKa = 4.18DD1564 pKa = 4.25LSKK1567 pKa = 10.97DD1568 pKa = 3.39FNMLVNKK1575 pKa = 10.33LFMTPPDD1582 pKa = 3.8YY1583 pKa = 10.91EE1584 pKa = 4.37LSFLGEE1590 pKa = 4.45LQWQWEE1596 pKa = 4.56TFFVKK1601 pKa = 10.03PLKK1604 pKa = 10.63LDD1606 pKa = 3.5IPDD1609 pKa = 4.64DD1610 pKa = 4.16FQPSLQYY1617 pKa = 11.1LPDD1620 pKa = 2.97VMMYY1624 pKa = 10.19FGINPPDD1631 pKa = 3.67NFEE1634 pKa = 4.05PTLDD1638 pKa = 3.96FFLMWGKK1645 pKa = 10.16EE1646 pKa = 3.9FMMKK1650 pKa = 10.37LGFVFPEE1657 pKa = 3.88RR1658 pKa = 11.84DD1659 pKa = 3.22MMGARR1664 pKa = 11.84DD1665 pKa = 4.83DD1666 pKa = 4.75DD1667 pKa = 4.05ATDD1670 pKa = 3.46AQAGDD1675 pKa = 3.94VMSSDD1680 pKa = 4.76DD1681 pKa = 3.92MMMMDD1686 pKa = 4.97FAFLEE1691 pKa = 4.25YY1692 pKa = 9.48MAEE1695 pKa = 4.04FFIINQNNWPMIIKK1709 pKa = 9.62EE1710 pKa = 4.34AEE1712 pKa = 3.95TAFPMIAAHH1721 pKa = 6.36FAEE1724 pKa = 5.35AGDD1727 pKa = 4.25KK1728 pKa = 10.6IAMTFMRR1735 pKa = 11.84DD1736 pKa = 3.17VNIILQAYY1744 pKa = 7.22TDD1746 pKa = 4.17YY1747 pKa = 9.55NTKK1750 pKa = 10.15VASNFMNLYY1759 pKa = 9.78EE1760 pKa = 4.85GIYY1763 pKa = 10.59DD1764 pKa = 3.99HH1765 pKa = 6.22VTMRR1769 pKa = 11.84AQVLVKK1775 pKa = 10.57KK1776 pKa = 10.11MMMYY1780 pKa = 9.98VSHH1783 pKa = 5.98MMKK1786 pKa = 10.48LGRR1789 pKa = 11.84HH1790 pKa = 5.41NFQAMVRR1797 pKa = 11.84HH1798 pKa = 5.33LRR1800 pKa = 11.84LRR1802 pKa = 11.84LSIEE1806 pKa = 3.84LHH1808 pKa = 5.76YY1809 pKa = 10.39LAHH1812 pKa = 6.69LAEE1815 pKa = 4.32EE1816 pKa = 4.52FMNNGNNYY1824 pKa = 9.74PILQAIAQQFQQPSHH1839 pKa = 7.08PMMNILSGFLDD1850 pKa = 4.67NYY1852 pKa = 10.0FQSQNKK1858 pKa = 9.07HH1859 pKa = 6.07PIAVLHH1865 pKa = 7.24DD1866 pKa = 4.14YY1867 pKa = 10.64LHH1869 pKa = 6.5SRR1871 pKa = 11.84PSVSTSRR1878 pKa = 11.84PLIDD1882 pKa = 4.89VIANYY1887 pKa = 10.33LDD1889 pKa = 3.89GMSNRR1894 pKa = 11.84PSRR1897 pKa = 11.84PILEE1901 pKa = 4.13QIMNYY1906 pKa = 10.01FSGQQQSAWDD1916 pKa = 3.79DD1917 pKa = 3.51VADD1920 pKa = 5.23FFFGMNNNNQRR1931 pKa = 11.84PLLQQLFDD1939 pKa = 4.03NFFGSNQQQYY1949 pKa = 10.11PVIQAIADD1957 pKa = 3.66NFFGNSQQQSSPVQMLMDD1975 pKa = 3.9YY1976 pKa = 11.46LNGQSNRR1983 pKa = 11.84PNMQSIVQQLLGSSSVINQLFGNNEE2008 pKa = 4.07RR2009 pKa = 11.84PTPQQMLYY2017 pKa = 10.02EE2018 pKa = 4.86HH2019 pKa = 7.21IFGNDD2024 pKa = 3.18GQRR2027 pKa = 11.84PQASLQVLIQQMFGTNRR2044 pKa = 11.84PSPNQIMQQAVQVMFGVQQQGWNTNTFYY2072 pKa = 11.27DD2073 pKa = 5.3DD2074 pKa = 5.52DD2075 pKa = 4.48NQDD2078 pKa = 3.41PLAWLLGDD2086 pKa = 5.28DD2087 pKa = 3.47WMNWFMPEE2095 pKa = 4.71EE2096 pKa = 4.21EE2097 pKa = 5.23EE2098 pKa = 4.29NDD2100 pKa = 3.29NWFSWLFEE2108 pKa = 4.29EE2109 pKa = 5.74EE2110 pKa = 5.26DD2111 pKa = 5.11KK2112 pKa = 11.61EE2113 pKa = 5.21DD2114 pKa = 5.56DD2115 pKa = 3.9NNDD2118 pKa = 2.98WFSWLMGDD2126 pKa = 3.68EE2127 pKa = 4.75DD2128 pKa = 5.1KK2129 pKa = 11.47SSEE2132 pKa = 3.98EE2133 pKa = 4.06DD2134 pKa = 5.37KK2135 pKa = 11.13EE2136 pKa = 4.68DD2137 pKa = 3.93SWLSWLFGEE2146 pKa = 5.4EE2147 pKa = 4.24DD2148 pKa = 4.05KK2149 pKa = 11.6EE2150 pKa = 4.62DD2151 pKa = 4.04DD2152 pKa = 5.51KK2153 pKa = 12.05EE2154 pKa = 6.51DD2155 pKa = 3.92DD2156 pKa = 3.91TQDD2159 pKa = 2.73WWSSWFGGEE2168 pKa = 4.16EE2169 pKa = 4.0EE2170 pKa = 4.72EE2171 pKa = 4.81EE2172 pKa = 4.42EE2173 pKa = 4.54KK2174 pKa = 11.19PEE2176 pKa = 3.64MWYY2179 pKa = 8.08TWFFTPPMEE2188 pKa = 4.02QDD2190 pKa = 3.16KK2191 pKa = 11.21DD2192 pKa = 3.91SSEE2195 pKa = 4.24SMEE2198 pKa = 4.29NNWLSWAQPQNDD2210 pKa = 4.16DD2211 pKa = 4.55QPDD2214 pKa = 3.67TFLTWWLSDD2223 pKa = 3.54DD2224 pKa = 3.54SQMYY2228 pKa = 10.49SGFDD2232 pKa = 3.35HH2233 pKa = 6.93LVKK2236 pKa = 10.52PMQFLRR2242 pKa = 11.84GRR2244 pKa = 11.84QWGG2247 pKa = 3.48

Molecular weight:
254.01 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|C3YYI9|C3YYI9_BRAFL Protein kinase domain-containing protein (Fragment) OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_118351 PE=4 SV=1
MM1 pKa = 7.46AALVILPIMPLRR13 pKa = 11.84ILPIMPLKK21 pKa = 10.34LLPIMPLRR29 pKa = 11.84SLHH32 pKa = 6.49IMPLKK37 pKa = 10.08ILPIMPLRR45 pKa = 11.84LLPIIPQGILPIMPQRR61 pKa = 11.84ILPIMPQRR69 pKa = 11.84ILPIMPQRR77 pKa = 11.84ILSLLLSMIPHH88 pKa = 7.46LALNMNQKK96 pKa = 10.25LLTMISNTLRR106 pKa = 11.84NQPLATTPLQPRR118 pKa = 11.84HH119 pKa = 5.63TIRR122 pKa = 11.84NRR124 pKa = 11.84GIRR127 pKa = 11.84RR128 pKa = 11.84RR129 pKa = 11.84LSTVIITT136 pKa = 3.85

Molecular weight:
15.55 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

28542

2

28544

13248417

49

25209

464.1

51.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.816 ± 0.014

2.499 ± 0.022

5.895 ± 0.022

6.348 ± 0.02

3.553 ± 0.01

6.824 ± 0.019

2.69 ± 0.009

4.333 ± 0.009

5.331 ± 0.019

8.628 ± 0.024

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.434 ± 0.008

3.987 ± 0.012

5.346 ± 0.017

4.43 ± 0.016

5.706 ± 0.015

7.477 ± 0.016

6.467 ± 0.025

6.8 ± 0.013

1.318 ± 0.006

3.116 ± 0.011

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski