Mycobacterium phage KayaCho

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; unclassified Bclasvirinae

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5YNA5|S5YNA5_9CAUD Uncharacterized protein OS=Mycobacterium phage KayaCho OX=1340830 GN=34 PE=4 SV=1
MM1 pKa = 6.88TQPTTVQWGVRR12 pKa = 11.84QEE14 pKa = 3.96VPIIPEE20 pKa = 4.05GEE22 pKa = 4.02MPEE25 pKa = 4.44QIPAPAPINYY35 pKa = 9.81EE36 pKa = 3.76GMTEE40 pKa = 4.06EE41 pKa = 4.13QVAEE45 pKa = 4.43AEE47 pKa = 4.13AQQAIDD53 pKa = 3.65YY54 pKa = 7.9AAWNAVMATWYY65 pKa = 10.48QDD67 pKa = 3.49VLALIAEE74 pKa = 4.43HH75 pKa = 7.15DD76 pKa = 4.04EE77 pKa = 3.49WWQYY81 pKa = 8.71TVATFPDD88 pKa = 3.77EE89 pKa = 4.02ATARR93 pKa = 11.84EE94 pKa = 4.08QLALTRR100 pKa = 11.84EE101 pKa = 4.34ANRR104 pKa = 11.84DD105 pKa = 3.31NAFVRR110 pKa = 11.84SVDD113 pKa = 3.81LVTAPVITWTVVEE126 pKa = 4.32

Molecular weight: 14.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5YNH9|S5YNH9_9CAUD Uncharacterized protein OS=Mycobacterium phage KayaCho OX=1340830 GN=89 PE=4 SV=1
MM1 pKa = 7.41SRR3 pKa = 11.84EE4 pKa = 3.91LLLPLEE10 pKa = 4.32LRR12 pKa = 11.84RR13 pKa = 11.84PPRR16 pKa = 11.84CPGSGEE22 pKa = 3.8WPHH25 pKa = 7.42RR26 pKa = 11.84NFKK29 pKa = 10.04GRR31 pKa = 11.84AEE33 pKa = 4.12CQVCGKK39 pKa = 10.2RR40 pKa = 11.84FAPKK44 pKa = 9.63LDD46 pKa = 3.17GRR48 pKa = 11.84VRR50 pKa = 11.84RR51 pKa = 11.84HH52 pKa = 5.52PAAPRR57 pKa = 11.84YY58 pKa = 9.82LPVRR62 pKa = 11.84VVDD65 pKa = 4.49IPDD68 pKa = 2.97

Molecular weight: 7.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

22503

50

1989

236.9

25.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.683 ± 0.404

1.004 ± 0.147

6.364 ± 0.234

6.119 ± 0.325

2.48 ± 0.106

9.421 ± 0.581

1.751 ± 0.162

4.235 ± 0.141

2.644 ± 0.181

8.048 ± 0.285

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.222 ± 0.108

2.706 ± 0.185

6.497 ± 0.248

3.48 ± 0.144

6.821 ± 0.346

4.933 ± 0.172

7.097 ± 0.243

7.355 ± 0.174

1.978 ± 0.15

2.164 ± 0.129
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski