Hydrangea chlorotic mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B3VKH5|B3VKH5_9VIRU Capsid protein OS=Hydrangea chlorotic mottle virus OX=375546 GN=CP PE=3 SV=2
MM1 pKa = 7.47WGDD4 pKa = 3.71PLSLVVSLLCFALTLYY20 pKa = 10.26FFYY23 pKa = 10.41PSEE26 pKa = 3.98QKK28 pKa = 10.27QCIITLTGEE37 pKa = 4.35SIRR40 pKa = 11.84IAGCEE45 pKa = 3.78LRR47 pKa = 11.84PEE49 pKa = 4.52HH50 pKa = 7.31IEE52 pKa = 4.42AIAKK56 pKa = 9.85LKK58 pKa = 10.59VLSAPLGEE66 pKa = 4.41QAA68 pKa = 5.25

Molecular weight: 7.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B3VKH4|B3VKH4_9VIRU 7 kDa protein OS=Hydrangea chlorotic mottle virus OX=375546 GN=TGB3 PE=3 SV=1
MM1 pKa = 7.91PLTPPPNFAQTYY13 pKa = 7.94LAAAVGISTAILVGLLTRR31 pKa = 11.84STLPHH36 pKa = 6.79VGDD39 pKa = 4.22LQHH42 pKa = 6.7SLPHH46 pKa = 6.05GGRR49 pKa = 11.84YY50 pKa = 8.92RR51 pKa = 11.84DD52 pKa = 3.63GTKK55 pKa = 10.43SVDD58 pKa = 3.3YY59 pKa = 10.78CSPRR63 pKa = 11.84KK64 pKa = 9.9LNSVEE69 pKa = 5.63RR70 pKa = 11.84GFQGQWLIWPLVIVLVGLVIFLSKK94 pKa = 10.42KK95 pKa = 10.11RR96 pKa = 11.84SNCYY100 pKa = 10.38ACGATHH106 pKa = 7.6

Molecular weight: 11.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2749

68

1949

458.2

51.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.057 ± 0.7

2.837 ± 0.639

4.765 ± 0.724

7.13 ± 0.914

5.02 ± 0.681

5.966 ± 0.5

2.692 ± 0.22

5.02 ± 0.769

6.366 ± 0.823

10.004 ± 0.96

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.437 ± 0.584

4.22 ± 0.88

4.292 ± 1.267

3.056 ± 0.22

6.148 ± 0.904

7.093 ± 0.516

4.365 ± 0.384

6.693 ± 0.252

1.091 ± 0.174

3.747 ± 0.433
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski