Giant panda circovirus 1
Average proteome isoelectric point is 8.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A220IGS1|A0A220IGS1_9CIRC Capsid protein OS=Giant panda circovirus 1 OX=2016456 PE=4 SV=1
MM1 pKa = 7.16 SQPEE5 pKa = 4.17 PLLSRR10 pKa = 11.84 SEE12 pKa = 4.01 ASEE15 pKa = 4.31 DD16 pKa = 3.37 GSRR19 pKa = 11.84 SSKK22 pKa = 9.74 SRR24 pKa = 11.84 RR25 pKa = 11.84 QGRR28 pKa = 11.84 YY29 pKa = 7.69 WILTISCDD37 pKa = 3.89 SNPWTPTALLPGQSYY52 pKa = 9.2 VVGQQEE58 pKa = 4.29 VGVGGFKK65 pKa = 9.95 HH66 pKa = 4.96 WQVMVAYY73 pKa = 8.97 SKK75 pKa = 10.95 KK76 pKa = 9.81 VGLRR80 pKa = 11.84 CVQRR84 pKa = 11.84 DD85 pKa = 3.84 FGSCHH90 pKa = 7.2 AEE92 pKa = 3.75 LTYY95 pKa = 10.89 SDD97 pKa = 4.26 SARR100 pKa = 11.84 EE101 pKa = 3.99 YY102 pKa = 10.6 CRR104 pKa = 11.84 KK105 pKa = 9.86 EE106 pKa = 3.75 DD107 pKa = 3.58 TRR109 pKa = 11.84 VEE111 pKa = 4.14 GTQFEE116 pKa = 4.92 FGTLATRR123 pKa = 11.84 RR124 pKa = 11.84 NSEE127 pKa = 4.0 TDD129 pKa = 2.44 WDD131 pKa = 4.46 VIRR134 pKa = 11.84 DD135 pKa = 3.75 AAQLSDD141 pKa = 3.81 LSGVPSDD148 pKa = 3.99 IYY150 pKa = 10.6 VRR152 pKa = 11.84 CYY154 pKa = 10.06 NQLRR158 pKa = 11.84 RR159 pKa = 11.84 IGQDD163 pKa = 2.71 HH164 pKa = 6.92 LRR166 pKa = 11.84 PVGMEE171 pKa = 4.04 RR172 pKa = 11.84 TCHH175 pKa = 5.14 VFWGRR180 pKa = 11.84 TGTGKK185 pKa = 9.99 SRR187 pKa = 11.84 RR188 pKa = 11.84 AWEE191 pKa = 3.97 EE192 pKa = 3.63 AGLCAYY198 pKa = 9.5 PKK200 pKa = 10.71 DD201 pKa = 4.16 PNTKK205 pKa = 9.51 FWDD208 pKa = 4.49 GYY210 pKa = 9.96 RR211 pKa = 11.84 NHH213 pKa = 5.95 EE214 pKa = 4.2 HH215 pKa = 6.16 VVIDD219 pKa = 3.79 EE220 pKa = 3.96 FRR222 pKa = 11.84 GIINISNLLRR232 pKa = 11.84 WLDD235 pKa = 3.8 RR236 pKa = 11.84 YY237 pKa = 9.87 PVIVEE242 pKa = 3.99 VKK244 pKa = 8.23 GSSVVFCARR253 pKa = 11.84 TIWITSNVNPEE264 pKa = 3.54 QWYY267 pKa = 9.59 PDD269 pKa = 3.59 ADD271 pKa = 3.92 QEE273 pKa = 4.72 TKK275 pKa = 10.77 DD276 pKa = 3.36 ALLRR280 pKa = 11.84 RR281 pKa = 11.84 LTVVYY286 pKa = 9.55 FEE288 pKa = 4.61
Molecular weight: 33.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.698
IPC2_protein 6.795
IPC_protein 6.868
Toseland 6.649
ProMoST 7.117
Dawson 7.468
Bjellqvist 7.585
Wikipedia 7.395
Rodwell 7.468
Grimsley 6.707
Solomon 7.527
Lehninger 7.541
Nozaki 7.892
DTASelect 7.658
Thurlkill 7.688
EMBOSS 7.732
Sillero 7.951
Patrickios 4.202
IPC_peptide 7.527
IPC2_peptide 7.337
IPC2.peptide.svr19 7.178
Protein with the highest isoelectric point:
>tr|A0A220IGS1|A0A220IGS1_9CIRC Capsid protein OS=Giant panda circovirus 1 OX=2016456 PE=4 SV=1
MM1 pKa = 7.45 VIGLMSLRR9 pKa = 11.84 PRR11 pKa = 11.84 YY12 pKa = 9.37 RR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 SITPSDD21 pKa = 3.57 VASFAWRR28 pKa = 11.84 NRR30 pKa = 11.84 RR31 pKa = 11.84 TVHH34 pKa = 6.47 NGILRR39 pKa = 11.84 SGEE42 pKa = 3.85 WVRR45 pKa = 11.84 RR46 pKa = 11.84 NAARR50 pKa = 11.84 RR51 pKa = 11.84 SSTSTVSTGGSRR63 pKa = 11.84 RR64 pKa = 11.84 SSLLSTGAASVKK76 pKa = 9.21 RR77 pKa = 11.84 TQLQGGATEE86 pKa = 3.79 RR87 pKa = 11.84 TARR90 pKa = 11.84 VKK92 pKa = 10.55 RR93 pKa = 11.84 YY94 pKa = 9.18 GSKK97 pKa = 10.14 LKK99 pKa = 10.6 KK100 pKa = 8.93 IGRR103 pKa = 11.84 RR104 pKa = 11.84 RR105 pKa = 11.84 VKK107 pKa = 10.24 VSRR110 pKa = 11.84 KK111 pKa = 8.98 LRR113 pKa = 11.84 AKK115 pKa = 10.15 IIEE118 pKa = 4.14 VTDD121 pKa = 3.71 GNKK124 pKa = 9.3 IQGYY128 pKa = 7.98 YY129 pKa = 8.77 QDD131 pKa = 3.95 NRR133 pKa = 11.84 IDD135 pKa = 3.72 VLDD138 pKa = 4.58 PGALGGRR145 pKa = 11.84 QNVEE149 pKa = 3.44 IFPNRR154 pKa = 11.84 GGAASGYY161 pKa = 7.71 MFNYY165 pKa = 10.11 DD166 pKa = 3.44 RR167 pKa = 11.84 VLHH170 pKa = 5.7 AASRR174 pKa = 11.84 LWNTKK179 pKa = 9.89 AAVQNPVYY187 pKa = 10.83 SDD189 pKa = 3.77 ALNFSPNDD197 pKa = 3.46 TVINVEE203 pKa = 4.2 KK204 pKa = 10.56 QWWTFEE210 pKa = 3.86 LRR212 pKa = 11.84 NNSTRR217 pKa = 11.84 TATLKK222 pKa = 9.24 FCKK225 pKa = 9.5 MRR227 pKa = 11.84 MKK229 pKa = 10.5 RR230 pKa = 11.84 GCAQTLPYY238 pKa = 8.89 DD239 pKa = 3.39 TWVNGLAQMTTSGEE253 pKa = 4.27 LVGGATINTMFTMPTLSTQFKK274 pKa = 8.26 TAYY277 pKa = 8.13 KK278 pKa = 10.36 LEE280 pKa = 4.4 VIKK283 pKa = 11.09 VVLEE287 pKa = 4.11 PGQSYY292 pKa = 8.14 TFNIEE297 pKa = 3.94 GPAMTYY303 pKa = 10.1 HH304 pKa = 6.2 GQDD307 pKa = 3.04 FFDD310 pKa = 3.76 NGTYY314 pKa = 10.23 RR315 pKa = 11.84 PVQKK319 pKa = 10.34 QDD321 pKa = 2.93 TLGFVIEE328 pKa = 4.35 HH329 pKa = 6.27 VDD331 pKa = 3.38 MVGTHH336 pKa = 5.64 ATSEE340 pKa = 4.36 GAAGNAGFAPDD351 pKa = 3.49 TTEE354 pKa = 4.41 ANQAQEE360 pKa = 4.01 RR361 pKa = 11.84 VYY363 pKa = 10.33 MRR365 pKa = 11.84 STYY368 pKa = 9.97 HH369 pKa = 6.49 CRR371 pKa = 11.84 LNMPEE376 pKa = 4.0 KK377 pKa = 10.84 VGGISTAAASIFQNGNRR394 pKa = 11.84 VRR396 pKa = 11.84 RR397 pKa = 11.84 TCVDD401 pKa = 2.73 EE402 pKa = 5.1 FMPTTFATTMHH413 pKa = 6.62 RR414 pKa = 11.84 RR415 pKa = 11.84 DD416 pKa = 3.58 EE417 pKa = 4.33 EE418 pKa = 4.44 NPVVDD423 pKa = 4.14 MNN425 pKa = 4.32
Molecular weight: 47.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 9.589
IPC_protein 10.189
Toseland 10.335
ProMoST 10.058
Dawson 10.511
Bjellqvist 10.218
Wikipedia 10.716
Rodwell 10.76
Grimsley 10.584
Solomon 10.57
Lehninger 10.526
Nozaki 10.335
DTASelect 10.204
Thurlkill 10.379
EMBOSS 10.745
Sillero 10.438
Patrickios 10.365
IPC_peptide 10.57
IPC2_peptide 9.107
IPC2.peptide.svr19 8.647
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
713
288
425
356.5
40.35
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.732 ± 1.102
1.683 ± 0.645
4.909 ± 0.79
5.189 ± 0.829
3.506 ± 0.225
7.854 ± 0.331
1.823 ± 0.153
3.927 ± 0.141
4.067 ± 0.35
5.891 ± 0.416
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.525 ± 0.873
4.909 ± 1.05
3.787 ± 0.224
4.208 ± 0.18
9.818 ± 0.056
7.293 ± 0.612
8.135 ± 1.314
7.854 ± 0.282
2.244 ± 0.723
3.647 ± 0.306
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here