Pedilanthus leaf curl virus [Pakistan:Multan:2004]
Average proteome isoelectric point is 8.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B7ZER7|B7ZER7_9GEMI C4 protein OS=Pedilanthus leaf curl virus [Pakistan:Multan:2004] OX=587313 GN=C4 PE=3 SV=1
MM1 pKa = 7.91 PFGTHH6 pKa = 6.6 YY7 pKa = 10.78 ILSTDD12 pKa = 3.02 IQVINIQNPIGTHH25 pKa = 4.86 LTKK28 pKa = 11.04 SMAPPKK34 pKa = 10.14 RR35 pKa = 11.84 FQIYY39 pKa = 9.45 ARR41 pKa = 11.84 NYY43 pKa = 7.9 FLTYY47 pKa = 7.93 PHH49 pKa = 7.15 CSLTKK54 pKa = 10.57 EE55 pKa = 4.09 EE56 pKa = 4.94 ALSQIQALDD65 pKa = 3.39 TPTNKK70 pKa = 9.87 KK71 pKa = 9.82 FIKK74 pKa = 9.65 ICRR77 pKa = 11.84 EE78 pKa = 3.71 LHH80 pKa = 6.25 EE81 pKa = 5.87 DD82 pKa = 4.22 GSPHH86 pKa = 6.11 LHH88 pKa = 6.16 VLIQFEE94 pKa = 4.46 GKK96 pKa = 9.71 YY97 pKa = 9.46 RR98 pKa = 11.84 CQNQRR103 pKa = 11.84 FFDD106 pKa = 4.04 LVSPTRR112 pKa = 11.84 SAHH115 pKa = 4.86 FHH117 pKa = 6.55 PNIQGAKK124 pKa = 9.25 SSSDD128 pKa = 3.11 VKK130 pKa = 11.17 SYY132 pKa = 11.26 LEE134 pKa = 4.04 KK135 pKa = 11.17 DD136 pKa = 3.12 GDD138 pKa = 3.87 TLEE141 pKa = 4.12 WGEE144 pKa = 3.84 FQIDD148 pKa = 3.21 GRR150 pKa = 11.84 SARR153 pKa = 11.84 GGQQSANDD161 pKa = 3.81 AYY163 pKa = 10.71 AAALNAGSKK172 pKa = 10.29 SEE174 pKa = 3.84 ALRR177 pKa = 11.84 VIKK180 pKa = 10.21 EE181 pKa = 4.18 LAPKK185 pKa = 10.3 DD186 pKa = 3.74 YY187 pKa = 11.2 VLQFHH192 pKa = 6.76 NLNANLDD199 pKa = 4.29 RR200 pKa = 11.84 IFTPPVEE207 pKa = 4.62 VYY209 pKa = 10.26 VSPFSSSSFDD219 pKa = 3.41 QVPEE223 pKa = 3.92 EE224 pKa = 4.34 LEE226 pKa = 3.73 VWAAEE231 pKa = 4.11 NVMDD235 pKa = 5.48 AAARR239 pKa = 11.84 PLRR242 pKa = 11.84 PKK244 pKa = 10.58 SIVIEE249 pKa = 4.33 GDD251 pKa = 3.1 SRR253 pKa = 11.84 TGKK256 pKa = 8.52 TMWARR261 pKa = 11.84 SLGPHH266 pKa = 6.71 NYY268 pKa = 10.18 LCGHH272 pKa = 7.38 LDD274 pKa = 4.18 LSPKK278 pKa = 10.02 VYY280 pKa = 10.89 SNEE283 pKa = 3.02 AWYY286 pKa = 10.82 NVIDD290 pKa = 5.29 DD291 pKa = 4.41 VDD293 pKa = 3.61 PHH295 pKa = 5.85 YY296 pKa = 11.0 LKK298 pKa = 10.7 HH299 pKa = 6.2 FKK301 pKa = 10.7 EE302 pKa = 4.46 FMGAQRR308 pKa = 11.84 DD309 pKa = 3.81 WQSNTKK315 pKa = 9.6 YY316 pKa = 10.57 GKK318 pKa = 9.12 PVQIKK323 pKa = 10.41 GGIPTIFLCNPGPNSSYY340 pKa = 11.34 KK341 pKa = 10.62 EE342 pKa = 3.85 FLDD345 pKa = 3.82 EE346 pKa = 4.66 EE347 pKa = 4.61 KK348 pKa = 11.18 NSALKK353 pKa = 10.53 NWALKK358 pKa = 10.24 NAIFVTLEE366 pKa = 3.94 GPLYY370 pKa = 10.17 PGSNQSAAQASQEE383 pKa = 3.81 GDD385 pKa = 3.32 QASTCC390 pKa = 3.84
Molecular weight: 43.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.003
IPC2_protein 5.97
IPC_protein 6.059
Toseland 6.465
ProMoST 6.465
Dawson 6.376
Bjellqvist 6.364
Wikipedia 6.402
Rodwell 6.376
Grimsley 6.62
Solomon 6.376
Lehninger 6.376
Nozaki 6.649
DTASelect 6.839
Thurlkill 6.868
EMBOSS 6.839
Sillero 6.766
Patrickios 4.062
IPC_peptide 6.402
IPC2_peptide 6.678
IPC2.peptide.svr19 6.685
Protein with the highest isoelectric point:
>tr|B7ZER4|B7ZER4_9GEMI Replication enhancer OS=Pedilanthus leaf curl virus [Pakistan:Multan:2004] OX=587313 GN=REn PE=3 SV=1
MM1 pKa = 7.71 SKK3 pKa = 10.35 RR4 pKa = 11.84 PADD7 pKa = 3.87 IIISTPASKK16 pKa = 10.3 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 2.99 SPYY27 pKa = 10.47 ASRR30 pKa = 11.84 AAAPIVRR37 pKa = 11.84 VTKK40 pKa = 10.52 ARR42 pKa = 11.84 AWANRR47 pKa = 11.84 PMNRR51 pKa = 11.84 KK52 pKa = 7.87 PRR54 pKa = 11.84 MYY56 pKa = 10.68 RR57 pKa = 11.84 MYY59 pKa = 10.44 RR60 pKa = 11.84 SPDD63 pKa = 3.21 VPRR66 pKa = 11.84 GCEE69 pKa = 4.34 GPCKK73 pKa = 10.01 VQSFEE78 pKa = 4.1 SRR80 pKa = 11.84 HH81 pKa = 6.13 DD82 pKa = 3.26 IQHH85 pKa = 6.35 IGKK88 pKa = 8.97 VMCVSDD94 pKa = 3.5 VTRR97 pKa = 11.84 GIGLTHH103 pKa = 6.67 RR104 pKa = 11.84 VGKK107 pKa = 9.66 RR108 pKa = 11.84 FCVKK112 pKa = 9.94 SVYY115 pKa = 10.52 VLGKK119 pKa = 9.41 IWMDD123 pKa = 3.35 EE124 pKa = 4.04 NIKK127 pKa = 9.32 TKK129 pKa = 10.6 NHH131 pKa = 5.78 TNSVMFFLVRR141 pKa = 11.84 DD142 pKa = 3.84 RR143 pKa = 11.84 RR144 pKa = 11.84 PVDD147 pKa = 3.13 KK148 pKa = 10.28 PQDD151 pKa = 3.56 FGEE154 pKa = 4.33 VFNMFDD160 pKa = 4.29 NEE162 pKa = 4.11 PSTATVKK169 pKa = 10.38 NVHH172 pKa = 6.5 RR173 pKa = 11.84 DD174 pKa = 3.15 RR175 pKa = 11.84 YY176 pKa = 8.77 QVLRR180 pKa = 11.84 KK181 pKa = 9.41 WYY183 pKa = 8.08 ATVTGGQYY191 pKa = 10.85 ASKK194 pKa = 10.24 EE195 pKa = 3.73 QALVKK200 pKa = 10.65 KK201 pKa = 9.58 FIRR204 pKa = 11.84 VNNYY208 pKa = 7.74 VVYY211 pKa = 9.96 NQQEE215 pKa = 3.8 AGKK218 pKa = 10.1 YY219 pKa = 8.23 EE220 pKa = 4.02 NHH222 pKa = 6.47 TEE224 pKa = 3.99 NALMLYY230 pKa = 7.52 MACTHH235 pKa = 7.06 ASNPVYY241 pKa = 9.86 ATLKK245 pKa = 9.47 IRR247 pKa = 11.84 IYY249 pKa = 10.67 FYY251 pKa = 11.26 DD252 pKa = 3.51 SVSNN256 pKa = 3.9
Molecular weight: 29.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.265
IPC2_protein 9.545
IPC_protein 9.765
Toseland 10.116
ProMoST 9.853
Dawson 10.335
Bjellqvist 10.028
Wikipedia 10.526
Rodwell 10.687
Grimsley 10.409
Solomon 10.365
Lehninger 10.321
Nozaki 10.145
DTASelect 10.028
Thurlkill 10.175
EMBOSS 10.526
Sillero 10.248
Patrickios 10.189
IPC_peptide 10.365
IPC2_peptide 8.887
IPC2.peptide.svr19 8.523
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1148
100
390
191.3
21.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.272 ± 0.739
2.265 ± 0.586
4.617 ± 0.419
4.791 ± 0.523
4.355 ± 0.388
5.226 ± 0.331
3.92 ± 0.759
5.401 ± 0.882
5.575 ± 0.651
6.969 ± 0.851
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.178 ± 0.455
5.314 ± 0.728
5.923 ± 0.463
5.314 ± 0.559
6.707 ± 1.167
8.362 ± 1.03
5.488 ± 0.57
6.359 ± 1.032
1.394 ± 0.239
3.571 ± 0.621
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here