Amino acid dipepetide frequency for Streptomyces sp. SW4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.544AlaAla: 22.544 ± 0.238
1.233AlaCys: 1.233 ± 0.041
8.404AlaAsp: 8.404 ± 0.108
9.248AlaGlu: 9.248 ± 0.164
3.485AlaPhe: 3.485 ± 0.073
14.3AlaGly: 14.3 ± 0.18
3.021AlaHis: 3.021 ± 0.06
3.229AlaIle: 3.229 ± 0.077
2.71AlaLys: 2.71 ± 0.064
14.619AlaLeu: 14.619 ± 0.161
2.6AlaMet: 2.6 ± 0.063
1.607AlaAsn: 1.607 ± 0.048
7.043AlaPro: 7.043 ± 0.131
3.656AlaGln: 3.656 ± 0.075
11.655AlaArg: 11.655 ± 0.159
5.752AlaSer: 5.752 ± 0.097
6.748AlaThr: 6.748 ± 0.094
12.751AlaVal: 12.751 ± 0.135
1.763AlaTrp: 1.763 ± 0.048
2.681AlaTyr: 2.681 ± 0.054
0.0AlaXaa: 0.0 ± 0.0
Cys
1.271CysAla: 1.271 ± 0.043
0.182CysCys: 0.182 ± 0.021
0.482CysAsp: 0.482 ± 0.023
0.462CysGlu: 0.462 ± 0.023
0.247CysPhe: 0.247 ± 0.017
1.144CysGly: 1.144 ± 0.044
0.2CysHis: 0.2 ± 0.018
0.159CysIle: 0.159 ± 0.013
0.113CysLys: 0.113 ± 0.011
0.737CysLeu: 0.737 ± 0.032
0.162CysMet: 0.162 ± 0.014
0.142CysAsn: 0.142 ± 0.013
0.613CysPro: 0.613 ± 0.03
0.153CysGln: 0.153 ± 0.013
0.838CysArg: 0.838 ± 0.038
0.534CysSer: 0.534 ± 0.03
0.541CysThr: 0.541 ± 0.026
0.692CysVal: 0.692 ± 0.027
0.166CysTrp: 0.166 ± 0.014
0.122CysTyr: 0.122 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
7.943AspAla: 7.943 ± 0.127
0.428AspCys: 0.428 ± 0.025
3.667AspAsp: 3.667 ± 0.083
3.883AspGlu: 3.883 ± 0.08
1.524AspPhe: 1.524 ± 0.042
6.504AspGly: 6.504 ± 0.097
1.391AspHis: 1.391 ± 0.043
1.663AspIle: 1.663 ± 0.05
1.205AspLys: 1.205 ± 0.048
6.058AspLeu: 6.058 ± 0.085
0.805AspMet: 0.805 ± 0.034
0.803AspAsn: 0.803 ± 0.033
4.482AspPro: 4.482 ± 0.083
1.409AspGln: 1.409 ± 0.045
5.259AspArg: 5.259 ± 0.083
2.166AspSer: 2.166 ± 0.057
3.015AspThr: 3.015 ± 0.059
4.768AspVal: 4.768 ± 0.085
0.936AspTrp: 0.936 ± 0.034
0.986AspTyr: 0.986 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
7.827GluAla: 7.827 ± 0.155
0.36GluCys: 0.36 ± 0.021
2.914GluAsp: 2.914 ± 0.065
3.974GluGlu: 3.974 ± 0.083
1.392GluPhe: 1.392 ± 0.048
4.359GluGly: 4.359 ± 0.071
1.577GluHis: 1.577 ± 0.044
2.308GluIle: 2.308 ± 0.06
1.652GluLys: 1.652 ± 0.048
6.654GluLeu: 6.654 ± 0.098
0.896GluMet: 0.896 ± 0.03
0.98GluAsn: 0.98 ± 0.038
3.543GluPro: 3.543 ± 0.071
2.227GluGln: 2.227 ± 0.052
5.974GluArg: 5.974 ± 0.094
2.332GluSer: 2.332 ± 0.058
2.894GluThr: 2.894 ± 0.071
4.623GluVal: 4.623 ± 0.081
0.747GluTrp: 0.747 ± 0.031
1.15GluTyr: 1.15 ± 0.04
0.0GluXaa: 0.0 ± 0.0
Phe
3.719PheAla: 3.719 ± 0.067
0.26PheCys: 0.26 ± 0.018
1.838PheAsp: 1.838 ± 0.055
1.472PheGlu: 1.472 ± 0.044
0.912PhePhe: 0.912 ± 0.039
2.951PheGly: 2.951 ± 0.07
0.58PheHis: 0.58 ± 0.029
0.65PheIle: 0.65 ± 0.029
0.444PheLys: 0.444 ± 0.023
2.546PheLeu: 2.546 ± 0.059
0.423PheMet: 0.423 ± 0.024
0.504PheAsn: 0.504 ± 0.024
1.277PhePro: 1.277 ± 0.039
0.658PheGln: 0.658 ± 0.033
2.004PheArg: 2.004 ± 0.049
1.317PheSer: 1.317 ± 0.045
1.899PheThr: 1.899 ± 0.059
2.189PheVal: 2.189 ± 0.052
0.374PheTrp: 0.374 ± 0.023
0.52PheTyr: 0.52 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
11.34GlyAla: 11.34 ± 0.155
0.984GlyCys: 0.984 ± 0.035
5.196GlyAsp: 5.196 ± 0.078
5.145GlyGlu: 5.145 ± 0.077
2.904GlyPhe: 2.904 ± 0.068
8.729GlyGly: 8.729 ± 0.154
2.407GlyHis: 2.407 ± 0.053
3.276GlyIle: 3.276 ± 0.068
2.219GlyLys: 2.219 ± 0.061
9.216GlyLeu: 9.216 ± 0.11
2.072GlyMet: 2.072 ± 0.055
1.604GlyAsn: 1.604 ± 0.041
5.729GlyPro: 5.729 ± 0.126
2.493GlyGln: 2.493 ± 0.061
9.035GlyArg: 9.035 ± 0.123
5.112GlySer: 5.112 ± 0.089
6.106GlyThr: 6.106 ± 0.1
7.61GlyVal: 7.61 ± 0.097
1.619GlyTrp: 1.619 ± 0.047
2.143GlyTyr: 2.143 ± 0.047
0.0GlyXaa: 0.0 ± 0.0
His
2.852HisAla: 2.852 ± 0.063
0.242HisCys: 0.242 ± 0.018
1.322HisAsp: 1.322 ± 0.042
1.215HisGlu: 1.215 ± 0.037
0.617HisPhe: 0.617 ± 0.03
2.636HisGly: 2.636 ± 0.065
0.639HisHis: 0.639 ± 0.033
0.63HisIle: 0.63 ± 0.029
0.396HisLys: 0.396 ± 0.021
2.33HisLeu: 2.33 ± 0.063
0.345HisMet: 0.345 ± 0.023
0.345HisAsn: 0.345 ± 0.021
1.836HisPro: 1.836 ± 0.049
0.604HisGln: 0.604 ± 0.026
2.398HisArg: 2.398 ± 0.058
0.905HisSer: 0.905 ± 0.033
1.313HisThr: 1.313 ± 0.04
1.784HisVal: 1.784 ± 0.048
0.37HisTrp: 0.37 ± 0.021
0.446HisTyr: 0.446 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
4.487IleAla: 4.487 ± 0.091
0.286IleCys: 0.286 ± 0.019
2.068IleAsp: 2.068 ± 0.051
1.868IleGlu: 1.868 ± 0.045
0.67IlePhe: 0.67 ± 0.031
3.402IleGly: 3.402 ± 0.071
0.581IleHis: 0.581 ± 0.025
0.846IleIle: 0.846 ± 0.039
0.699IleLys: 0.699 ± 0.035
2.334IleLeu: 2.334 ± 0.056
0.461IleMet: 0.461 ± 0.025
0.65IleAsn: 0.65 ± 0.032
1.556IlePro: 1.556 ± 0.047
0.646IleGln: 0.646 ± 0.03
2.221IleArg: 2.221 ± 0.049
1.422IleSer: 1.422 ± 0.045
2.02IleThr: 2.02 ± 0.054
2.651IleVal: 2.651 ± 0.065
0.314IleTrp: 0.314 ± 0.018
0.467IleTyr: 0.467 ± 0.025
0.0IleXaa: 0.0 ± 0.0
Lys
2.999LysAla: 2.999 ± 0.073
0.11LysCys: 0.11 ± 0.014
1.256LysAsp: 1.256 ± 0.048
1.277LysGlu: 1.277 ± 0.048
0.423LysPhe: 0.423 ± 0.022
1.72LysGly: 1.72 ± 0.052
0.404LysHis: 0.404 ± 0.026
0.869LysIle: 0.869 ± 0.033
0.952LysLys: 0.952 ± 0.047
1.977LysLeu: 1.977 ± 0.061
0.404LysMet: 0.404 ± 0.025
0.507LysAsn: 0.507 ± 0.029
1.255LysPro: 1.255 ± 0.043
0.699LysGln: 0.699 ± 0.032
1.556LysArg: 1.556 ± 0.049
1.02LysSer: 1.02 ± 0.043
1.18LysThr: 1.18 ± 0.041
1.857LysVal: 1.857 ± 0.059
0.213LysTrp: 0.213 ± 0.02
0.42LysTyr: 0.42 ± 0.026
0.0LysXaa: 0.0 ± 0.0
Leu
15.063LeuAla: 15.063 ± 0.171
0.867LeuCys: 0.867 ± 0.033
6.527LeuAsp: 6.527 ± 0.102
4.965LeuGlu: 4.965 ± 0.092
2.512LeuPhe: 2.512 ± 0.062
8.85LeuGly: 8.85 ± 0.114
2.191LeuHis: 2.191 ± 0.047
3.006LeuIle: 3.006 ± 0.062
2.026LeuLys: 2.026 ± 0.057
11.248LeuLeu: 11.248 ± 0.172
1.692LeuMet: 1.692 ± 0.047
1.487LeuAsn: 1.487 ± 0.044
6.324LeuPro: 6.324 ± 0.094
2.084LeuGln: 2.084 ± 0.048
8.941LeuArg: 8.941 ± 0.115
4.889LeuSer: 4.889 ± 0.082
6.608LeuThr: 6.608 ± 0.093
8.883LeuVal: 8.883 ± 0.114
1.26LeuTrp: 1.26 ± 0.046
1.779LeuTyr: 1.779 ± 0.046
0.0LeuXaa: 0.0 ± 0.0
Met
2.354MetAla: 2.354 ± 0.047
0.149MetCys: 0.149 ± 0.015
0.862MetAsp: 0.862 ± 0.036
0.781MetGlu: 0.781 ± 0.032
0.504MetPhe: 0.504 ± 0.028
1.337MetGly: 1.337 ± 0.04
0.339MetHis: 0.339 ± 0.021
0.689MetIle: 0.689 ± 0.03
0.427MetLys: 0.427 ± 0.022
1.679MetLeu: 1.679 ± 0.047
0.311MetMet: 0.311 ± 0.021
0.414MetAsn: 0.414 ± 0.021
1.173MetPro: 1.173 ± 0.039
0.491MetGln: 0.491 ± 0.025
1.562MetArg: 1.562 ± 0.044
1.34MetSer: 1.34 ± 0.041
1.604MetThr: 1.604 ± 0.043
1.334MetVal: 1.334 ± 0.044
0.222MetTrp: 0.222 ± 0.016
0.314MetTyr: 0.314 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
1.963AsnAla: 1.963 ± 0.055
0.17AsnCys: 0.17 ± 0.015
0.866AsnAsp: 0.866 ± 0.035
0.801AsnGlu: 0.801 ± 0.034
0.408AsnPhe: 0.408 ± 0.024
1.65AsnGly: 1.65 ± 0.05
0.365AsnHis: 0.365 ± 0.023
0.593AsnIle: 0.593 ± 0.027
0.439AsnLys: 0.439 ± 0.026
1.444AsnLeu: 1.444 ± 0.046
0.263AsnMet: 0.263 ± 0.02
0.383AsnAsn: 0.383 ± 0.023
1.247AsnPro: 1.247 ± 0.04
0.438AsnGln: 0.438 ± 0.025
1.206AsnArg: 1.206 ± 0.044
0.795AsnSer: 0.795 ± 0.036
0.937AsnThr: 0.937 ± 0.036
1.301AsnVal: 1.301 ± 0.043
0.268AsnTrp: 0.268 ± 0.018
0.351AsnTyr: 0.351 ± 0.02
0.0AsnXaa: 0.0 ± 0.0
Pro
9.395ProAla: 9.395 ± 0.177
0.496ProCys: 0.496 ± 0.027
4.427ProAsp: 4.427 ± 0.086
4.244ProGlu: 4.244 ± 0.079
1.441ProPhe: 1.441 ± 0.042
7.221ProGly: 7.221 ± 0.116
1.531ProHis: 1.531 ± 0.043
1.133ProIle: 1.133 ± 0.04
0.996ProLys: 0.996 ± 0.042
5.067ProLeu: 5.067 ± 0.078
1.054ProMet: 1.054 ± 0.039
0.858ProAsn: 0.858 ± 0.033
4.028ProPro: 4.028 ± 0.107
1.581ProGln: 1.581 ± 0.048
4.801ProArg: 4.801 ± 0.091
3.187ProSer: 3.187 ± 0.077
3.058ProThr: 3.058 ± 0.069
5.557ProVal: 5.557 ± 0.092
0.872ProTrp: 0.872 ± 0.035
1.351ProTyr: 1.351 ± 0.043
0.0ProXaa: 0.0 ± 0.0
Gln
3.708GlnAla: 3.708 ± 0.071
0.175GlnCys: 0.175 ± 0.018
1.383GlnAsp: 1.383 ± 0.046
1.544GlnGlu: 1.544 ± 0.039
0.607GlnPhe: 0.607 ± 0.028
2.147GlnGly: 2.147 ± 0.059
0.657GlnHis: 0.657 ± 0.032
1.018GlnIle: 1.018 ± 0.037
0.589GlnLys: 0.589 ± 0.034
2.756GlnLeu: 2.756 ± 0.056
0.469GlnMet: 0.469 ± 0.023
0.449GlnAsn: 0.449 ± 0.026
1.594GlnPro: 1.594 ± 0.049
1.191GlnGln: 1.191 ± 0.051
2.433GlnArg: 2.433 ± 0.055
1.113GlnSer: 1.113 ± 0.044
1.227GlnThr: 1.227 ± 0.044
2.185GlnVal: 2.185 ± 0.052
0.406GlnTrp: 0.406 ± 0.021
0.557GlnTyr: 0.557 ± 0.029
0.0GlnXaa: 0.0 ± 0.0
Arg
11.187ArgAla: 11.187 ± 0.146
0.841ArgCys: 0.841 ± 0.036
4.723ArgAsp: 4.723 ± 0.078
5.152ArgGlu: 5.152 ± 0.089
2.322ArgPhe: 2.322 ± 0.055
6.281ArgGly: 6.281 ± 0.106
2.456ArgHis: 2.456 ± 0.057
3.222ArgIle: 3.222 ± 0.069
1.801ArgLys: 1.801 ± 0.052
9.398ArgLeu: 9.398 ± 0.125
1.953ArgMet: 1.953 ± 0.047
1.355ArgAsn: 1.355 ± 0.033
6.303ArgPro: 6.303 ± 0.137
2.365ArgGln: 2.365 ± 0.051
10.015ArgArg: 10.015 ± 0.185
4.289ArgSer: 4.289 ± 0.076
6.025ArgThr: 6.025 ± 0.096
6.361ArgVal: 6.361 ± 0.087
1.446ArgTrp: 1.446 ± 0.044
1.716ArgTyr: 1.716 ± 0.047
0.0ArgXaa: 0.0 ± 0.0
Ser
6.439SerAla: 6.439 ± 0.098
0.493SerCys: 0.493 ± 0.028
2.392SerAsp: 2.392 ± 0.053
2.134SerGlu: 2.134 ± 0.059
1.439SerPhe: 1.439 ± 0.047
5.573SerGly: 5.573 ± 0.108
0.941SerHis: 0.941 ± 0.033
1.258SerIle: 1.258 ± 0.05
0.938SerLys: 0.938 ± 0.041
4.546SerLeu: 4.546 ± 0.078
1.054SerMet: 1.054 ± 0.039
0.742SerAsn: 0.742 ± 0.03
3.106SerPro: 3.106 ± 0.088
1.078SerGln: 1.078 ± 0.036
3.969SerArg: 3.969 ± 0.077
2.856SerSer: 2.856 ± 0.088
2.808SerThr: 2.808 ± 0.076
4.09SerVal: 4.09 ± 0.076
0.887SerTrp: 0.887 ± 0.032
1.055SerTyr: 1.055 ± 0.039
0.0SerXaa: 0.0 ± 0.0
Thr
8.821ThrAla: 8.821 ± 0.113
0.453ThrCys: 0.453 ± 0.021
3.419ThrAsp: 3.419 ± 0.065
3.3ThrGlu: 3.3 ± 0.074
1.546ThrPhe: 1.546 ± 0.044
6.637ThrGly: 6.637 ± 0.094
1.18ThrHis: 1.18 ± 0.041
1.523ThrIle: 1.523 ± 0.051
1.095ThrLys: 1.095 ± 0.042
5.39ThrLeu: 5.39 ± 0.086
0.924ThrMet: 0.924 ± 0.032
0.873ThrAsn: 0.873 ± 0.037
4.035ThrPro: 4.035 ± 0.08
1.208ThrGln: 1.208 ± 0.036
4.276ThrArg: 4.276 ± 0.074
2.984ThrSer: 2.984 ± 0.072
3.676ThrThr: 3.676 ± 0.078
5.949ThrVal: 5.949 ± 0.097
0.866ThrTrp: 0.866 ± 0.036
1.211ThrTyr: 1.211 ± 0.042
0.0ThrXaa: 0.0 ± 0.0
Val
10.9ValAla: 10.9 ± 0.113
0.866ValCys: 0.866 ± 0.031
4.998ValAsp: 4.998 ± 0.082
4.874ValGlu: 4.874 ± 0.085
2.493ValPhe: 2.493 ± 0.058
6.416ValGly: 6.416 ± 0.1
2.037ValHis: 2.037 ± 0.054
2.63ValIle: 2.63 ± 0.067
1.71ValLys: 1.71 ± 0.057
9.485ValLeu: 9.485 ± 0.11
1.427ValMet: 1.427 ± 0.044
1.503ValAsn: 1.503 ± 0.046
5.37ValPro: 5.37 ± 0.082
2.069ValGln: 2.069 ± 0.06
7.873ValArg: 7.873 ± 0.107
4.081ValSer: 4.081 ± 0.07
5.639ValThr: 5.639 ± 0.079
8.121ValVal: 8.121 ± 0.102
1.12ValTrp: 1.12 ± 0.043
1.652ValTyr: 1.652 ± 0.047
0.0ValXaa: 0.0 ± 0.0
Trp
1.598TrpAla: 1.598 ± 0.05
0.192TrpCys: 0.192 ± 0.015
0.732TrpAsp: 0.732 ± 0.032
0.707TrpGlu: 0.707 ± 0.029
0.519TrpPhe: 0.519 ± 0.029
0.952TrpGly: 0.952 ± 0.032
0.355TrpHis: 0.355 ± 0.022
0.51TrpIle: 0.51 ± 0.03
0.323TrpLys: 0.323 ± 0.02
1.715TrpLeu: 1.715 ± 0.054
0.309TrpMet: 0.309 ± 0.018
0.334TrpAsn: 0.334 ± 0.021
0.797TrpPro: 0.797 ± 0.036
0.578TrpGln: 0.578 ± 0.028
1.379TrpArg: 1.379 ± 0.044
0.846TrpSer: 0.846 ± 0.033
1.005TrpThr: 1.005 ± 0.035
1.0TrpVal: 1.0 ± 0.037
0.332TrpTrp: 0.332 ± 0.021
0.311TrpTyr: 0.311 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.639TyrAla: 2.639 ± 0.06
0.175TyrCys: 0.175 ± 0.013
1.42TyrAsp: 1.42 ± 0.038
1.337TyrGlu: 1.337 ± 0.05
0.573TyrPhe: 0.573 ± 0.025
2.132TyrGly: 2.132 ± 0.049
0.374TyrHis: 0.374 ± 0.021
0.434TyrIle: 0.434 ± 0.025
0.364TyrLys: 0.364 ± 0.022
2.002TyrLeu: 2.002 ± 0.053
0.261TyrMet: 0.261 ± 0.019
0.346TyrAsn: 0.346 ± 0.022
0.989TyrPro: 0.989 ± 0.032
0.58TyrGln: 0.58 ± 0.028
1.818TyrArg: 1.818 ± 0.048
0.858TyrSer: 0.858 ± 0.032
1.063TyrThr: 1.063 ± 0.035
1.557TyrVal: 1.557 ± 0.043
0.314TyrTrp: 0.314 ± 0.018
0.406TyrTyr: 0.406 ± 0.024
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3180 proteins (811002 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski