Sphingomonas phage Scott

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Scottvirus; Sphingomonas virus Scott

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A346FDC2|A0A346FDC2_9CAUD Internal virion protein OS=Sphingomonas phage Scott OX=2282912 GN=SPS_25 PE=4 SV=1
MM1 pKa = 8.12ADD3 pKa = 3.19TDD5 pKa = 4.44LYY7 pKa = 11.06SVNIFPGNGTQTNFEE22 pKa = 3.84ISFAGGYY29 pKa = 9.5ISRR32 pKa = 11.84EE33 pKa = 3.79HH34 pKa = 5.87VKK36 pKa = 10.28AYY38 pKa = 10.83LRR40 pKa = 11.84IGQGAEE46 pKa = 4.16TPVSLEE52 pKa = 3.79WMDD55 pKa = 4.5DD56 pKa = 3.17NVVRR60 pKa = 11.84YY61 pKa = 7.8TPAPPLNSVLVVYY74 pKa = 10.79RR75 pKa = 11.84EE76 pKa = 4.11TPKK79 pKa = 10.92DD80 pKa = 3.28KK81 pKa = 10.81PLADD85 pKa = 4.72FSNGAVLTEE94 pKa = 4.0SSLDD98 pKa = 3.55INAKK102 pKa = 8.65QAVFIAAEE110 pKa = 4.11AQDD113 pKa = 3.86NGTEE117 pKa = 4.15RR118 pKa = 11.84ITNVEE123 pKa = 3.95KK124 pKa = 11.01QSIKK128 pKa = 10.56SVNGGLVGSGFVVVGSDD145 pKa = 3.87GVASGQDD152 pKa = 3.2FLNIVKK158 pKa = 8.91TATPVADD165 pKa = 4.27DD166 pKa = 4.63GAWGSAFADD175 pKa = 3.8DD176 pKa = 5.04GVWGG180 pKa = 3.96

Molecular weight: 19.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A346FDA1|A0A346FDA1_9CAUD Uncharacterized protein OS=Sphingomonas phage Scott OX=2282912 GN=SPS_4 PE=4 SV=1
MM1 pKa = 7.51TSLLLRR7 pKa = 11.84GTRR10 pKa = 11.84ALVSLIVSLHH20 pKa = 5.11VLLLRR25 pKa = 11.84FTTVLADD32 pKa = 3.74RR33 pKa = 11.84KK34 pKa = 9.56VAKK37 pKa = 9.64NDD39 pKa = 2.85KK40 pKa = 10.09RR41 pKa = 11.84VRR43 pKa = 11.84QAQNGVEE50 pKa = 4.66FARR53 pKa = 11.84QALQYY58 pKa = 10.54AKK60 pKa = 10.78LSVRR64 pKa = 11.84EE65 pKa = 4.52FKK67 pKa = 10.44DD68 pKa = 3.02QHH70 pKa = 7.85IIAQNEE76 pKa = 3.76ARR78 pKa = 11.84NTRR81 pKa = 11.84VAADD85 pKa = 3.97AEE87 pKa = 4.87CKK89 pKa = 10.54FFGRR93 pKa = 11.84SLL95 pKa = 3.44

Molecular weight: 10.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

13170

38

1299

258.2

28.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.078 ± 0.49

0.896 ± 0.173

6.31 ± 0.207

5.414 ± 0.282

3.584 ± 0.186

8.519 ± 0.436

1.549 ± 0.185

4.692 ± 0.176

5.133 ± 0.317

8.307 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.756 ± 0.199

4.252 ± 0.204

4.457 ± 0.223

4.184 ± 0.261

6.052 ± 0.335

5.611 ± 0.308

5.915 ± 0.331

6.697 ± 0.311

1.503 ± 0.165

3.09 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski