Gigaspora margarita mitovirus 3
Average proteome isoelectric point is 8.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2L0VZH5|A0A2L0VZH5_9VIRU RNA-dependent RNA polymerase OS=Gigaspora margarita mitovirus 3 OX=2082667 PE=4 SV=1
MM1 pKa = 7.48 IRR3 pKa = 11.84 LLINGPVPFGVTRR16 pKa = 11.84 GAVNLSLINLQNALFSTSCVILRR39 pKa = 11.84 KK40 pKa = 10.2 YY41 pKa = 8.97 EE42 pKa = 3.77 VWTNLAKK49 pKa = 10.58 RR50 pKa = 11.84 HH51 pKa = 5.09 GFAPAEE57 pKa = 4.23 VIEE60 pKa = 4.32 ASALGAYY67 pKa = 9.18 VVVDD71 pKa = 3.86 PMNKK75 pKa = 9.66 SSLLALSPMAYY86 pKa = 9.59 QKK88 pKa = 11.44 LLGQTMSTEE97 pKa = 4.0 NLSLILLLSPLDD109 pKa = 3.64 EE110 pKa = 4.89 LEE112 pKa = 4.23 RR113 pKa = 11.84 SAAEE117 pKa = 4.09 ASPEE121 pKa = 4.05 ATKK124 pKa = 10.8 SPPLKK129 pKa = 10.69 GFVNKK134 pKa = 10.18 ISLDD138 pKa = 3.6 LEE140 pKa = 4.09 RR141 pKa = 11.84 LKK143 pKa = 11.05 EE144 pKa = 4.22 RR145 pKa = 11.84 IQTGRR150 pKa = 11.84 LTKK153 pKa = 10.21 GVRR156 pKa = 11.84 WPSWSLGYY164 pKa = 9.67 PFQGYY169 pKa = 9.29 QGLGCRR175 pKa = 11.84 LSDD178 pKa = 3.4 GRR180 pKa = 11.84 LILSRR185 pKa = 11.84 PSEE188 pKa = 4.05 FSAIFQRR195 pKa = 11.84 WASRR199 pKa = 11.84 LHH201 pKa = 5.58 FWSGSSRR208 pKa = 11.84 GRR210 pKa = 11.84 RR211 pKa = 11.84 FRR213 pKa = 11.84 SSLWVVTNHH222 pKa = 5.85 LQTVLKK228 pKa = 10.71 HH229 pKa = 5.53 SGPYY233 pKa = 10.23 GLALYY238 pKa = 10.25 LKK240 pKa = 9.8 ASYY243 pKa = 10.66 NIVLRR248 pKa = 11.84 YY249 pKa = 9.6 VAGQPFEE256 pKa = 4.58 HH257 pKa = 7.22 AFTQCGVPVQLQNGLPKK274 pKa = 10.4 FLPRR278 pKa = 11.84 EE279 pKa = 3.69 WRR281 pKa = 11.84 VAIRR285 pKa = 11.84 SGSPRR290 pKa = 11.84 LLRR293 pKa = 11.84 IVLSLLYY300 pKa = 10.18 SYY302 pKa = 11.16 KK303 pKa = 10.53 GFKK306 pKa = 8.19 ATKK309 pKa = 8.28 PKK311 pKa = 10.09 PVAEE315 pKa = 3.9 IFKK318 pKa = 10.25 NISLAAHH325 pKa = 5.9 QPDD328 pKa = 5.36 DD329 pKa = 4.43 IIAFSKK335 pKa = 10.84 FCFEE339 pKa = 4.04 FLKK342 pKa = 11.0 NRR344 pKa = 11.84 ISPISEE350 pKa = 4.05 GEE352 pKa = 4.17 LNPPSLPASLKK363 pKa = 10.54 AGPNGPLSALAYY375 pKa = 10.34 GADD378 pKa = 3.43 AYY380 pKa = 10.9 AWIQRR385 pKa = 11.84 EE386 pKa = 4.13 SAPYY390 pKa = 8.84 QPSVPGPLEE399 pKa = 4.03 WVQHH403 pKa = 5.8 FGLDD407 pKa = 2.98 KK408 pKa = 10.24 WEE410 pKa = 4.29 KK411 pKa = 10.44 KK412 pKa = 9.31 FRR414 pKa = 11.84 AYY416 pKa = 10.95 SDD418 pKa = 3.15 WFLKK422 pKa = 10.62 RR423 pKa = 11.84 GFNHH427 pKa = 5.57 QFNVTFPPPTRR438 pKa = 11.84 KK439 pKa = 10.17 GFGVLKK445 pKa = 10.54 LKK447 pKa = 10.41 IALGLNVPEE456 pKa = 4.78 SKK458 pKa = 10.72 SIALNPKK465 pKa = 8.92 FAFHH469 pKa = 7.04 RR470 pKa = 11.84 RR471 pKa = 11.84 TVSYY475 pKa = 10.0 TPTRR479 pKa = 11.84 VEE481 pKa = 4.24 DD482 pKa = 4.27 VEE484 pKa = 5.01 ALQCLGLLLPVGRR497 pKa = 11.84 VHH499 pKa = 6.76 VLWEE503 pKa = 4.0 AAGKK507 pKa = 7.82 TRR509 pKa = 11.84 VIAMMDD515 pKa = 3.94 GLRR518 pKa = 11.84 QALLRR523 pKa = 11.84 PVHH526 pKa = 6.15 LAMFRR531 pKa = 11.84 RR532 pKa = 11.84 IDD534 pKa = 3.83 KK535 pKa = 10.84 IFGFCSGLYY544 pKa = 9.18 DD545 pKa = 3.08 QSGAVRR551 pKa = 11.84 SFAALNNRR559 pKa = 11.84 EE560 pKa = 4.09 VFSYY564 pKa = 10.47 DD565 pKa = 2.51 ISAATDD571 pKa = 3.86 TIPYY575 pKa = 9.76 QLYY578 pKa = 8.91 FPLMEE583 pKa = 4.55 FLLGKK588 pKa = 8.87 TGAVLWNHH596 pKa = 5.95 MVRR599 pKa = 11.84 GHH601 pKa = 6.33 PFIHH605 pKa = 6.93 LEE607 pKa = 4.33 GGDD610 pKa = 3.92 EE611 pKa = 3.95 PWAQRR616 pKa = 11.84 LAGSSSYY623 pKa = 10.82 YY624 pKa = 9.26 YY625 pKa = 10.61 EE626 pKa = 4.96 RR627 pKa = 11.84 GQPMGGYY634 pKa = 9.96 SSFAALDD641 pKa = 3.84 MLHH644 pKa = 6.74 HH645 pKa = 7.09 LIVQYY650 pKa = 10.96 SAFEE654 pKa = 4.12 AGYY657 pKa = 8.37 DD658 pKa = 3.78 TLNKK662 pKa = 9.88 TFDD665 pKa = 3.26 NYY667 pKa = 10.83 RR668 pKa = 11.84 ILGDD672 pKa = 3.74 DD673 pKa = 3.63 VVIGDD678 pKa = 3.48 VRR680 pKa = 11.84 VAKK683 pKa = 9.91 TYY685 pKa = 11.11 LNFMRR690 pKa = 11.84 TWNIPISEE698 pKa = 4.25 NKK700 pKa = 10.33 SLVSSKK706 pKa = 11.01 GVFQFLSEE714 pKa = 4.01 VFRR717 pKa = 11.84 GGEE720 pKa = 4.15 CLSPLSFRR728 pKa = 11.84 ADD730 pKa = 3.64 YY731 pKa = 10.43 QSKK734 pKa = 8.02 TFLEE738 pKa = 4.41 RR739 pKa = 11.84 FSFAATAVSRR749 pKa = 11.84 GWSSGLAMEE758 pKa = 5.02 GFGRR762 pKa = 11.84 LILSKK767 pKa = 10.89 DD768 pKa = 3.27 QFLSEE773 pKa = 3.22 IHH775 pKa = 7.07 RR776 pKa = 11.84 YY777 pKa = 8.66 RR778 pKa = 11.84 NRR780 pKa = 11.84 RR781 pKa = 11.84 PSAFIYY787 pKa = 10.59 SLLFALVTFAGEE799 pKa = 4.18 AGWALGSWLHH809 pKa = 5.92 NVVAHH814 pKa = 7.25 DD815 pKa = 4.96 PFPQLLSNKK824 pKa = 7.69 EE825 pKa = 4.09 LEE827 pKa = 4.48 SVEE830 pKa = 4.22 SKK832 pKa = 11.2 NFVFTILRR840 pKa = 11.84 QILFTEE846 pKa = 4.72 AGRR849 pKa = 11.84 IHH851 pKa = 6.98 EE852 pKa = 4.19 LQKK855 pKa = 11.11 AEE857 pKa = 4.16 SGVNQTRR864 pKa = 11.84 QFLDD868 pKa = 3.71 FGSIEE873 pKa = 3.91 FHH875 pKa = 6.95 NDD877 pKa = 2.19 IEE879 pKa = 4.72 NDD881 pKa = 3.43 VDD883 pKa = 4.9 SLWLLARR890 pKa = 11.84 EE891 pKa = 4.43 GKK893 pKa = 8.11 TLLEE897 pKa = 4.55 SADD900 pKa = 3.74 VTPDD904 pKa = 2.7 WVTSAFSYY912 pKa = 9.98 LRR914 pKa = 11.84 KK915 pKa = 9.97 VSGIIPRR922 pKa = 11.84 LSATGVVHH930 pKa = 6.72 PLDD933 pKa = 3.88 YY934 pKa = 10.98 LVLMKK939 pKa = 8.87 TTEE942 pKa = 3.97 DD943 pKa = 3.29 RR944 pKa = 11.84 NNIHH948 pKa = 7.14 KK949 pKa = 10.22 VNQWSPTNHH958 pKa = 6.65 DD959 pKa = 3.27 AEE961 pKa = 4.62 EE962 pKa = 4.07 IFRR965 pKa = 11.84 VRR967 pKa = 11.84 MVDD970 pKa = 3.26 TTFSLIKK977 pKa = 10.4 SNPYY981 pKa = 8.01 YY982 pKa = 10.8 HH983 pKa = 7.13 EE984 pKa = 4.31 VLTGISSPYY993 pKa = 10.21 GLALRR998 pKa = 11.84 PVPEE1002 pKa = 4.27 EE1003 pKa = 3.89 GRR1005 pKa = 11.84 PSGSSS1010 pKa = 2.91
Molecular weight: 113.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.819
IPC2_protein 8.873
IPC_protein 8.799
Toseland 9.341
ProMoST 9.224
Dawson 9.677
Bjellqvist 9.443
Wikipedia 9.882
Rodwell 9.853
Grimsley 9.78
Solomon 9.706
Lehninger 9.648
Nozaki 9.443
DTASelect 9.414
Thurlkill 9.502
EMBOSS 9.809
Sillero 9.619
Patrickios 4.762
IPC_peptide 9.692
IPC2_peptide 8.083
IPC2.peptide.svr19 7.779
Protein with the highest isoelectric point:
>tr|A0A2L0VZH5|A0A2L0VZH5_9VIRU RNA-dependent RNA polymerase OS=Gigaspora margarita mitovirus 3 OX=2082667 PE=4 SV=1
MM1 pKa = 7.48 IRR3 pKa = 11.84 LLINGPVPFGVTRR16 pKa = 11.84 GAVNLSLINLQNALFSTSCVILRR39 pKa = 11.84 KK40 pKa = 10.2 YY41 pKa = 8.97 EE42 pKa = 3.77 VWTNLAKK49 pKa = 10.58 RR50 pKa = 11.84 HH51 pKa = 5.09 GFAPAEE57 pKa = 4.23 VIEE60 pKa = 4.32 ASALGAYY67 pKa = 9.18 VVVDD71 pKa = 3.86 PMNKK75 pKa = 9.66 SSLLALSPMAYY86 pKa = 9.59 QKK88 pKa = 11.44 LLGQTMSTEE97 pKa = 4.0 NLSLILLLSPLDD109 pKa = 3.64 EE110 pKa = 4.89 LEE112 pKa = 4.23 RR113 pKa = 11.84 SAAEE117 pKa = 4.09 ASPEE121 pKa = 4.05 ATKK124 pKa = 10.8 SPPLKK129 pKa = 10.69 GFVNKK134 pKa = 10.18 ISLDD138 pKa = 3.6 LEE140 pKa = 4.09 RR141 pKa = 11.84 LKK143 pKa = 11.05 EE144 pKa = 4.22 RR145 pKa = 11.84 IQTGRR150 pKa = 11.84 LTKK153 pKa = 10.21 GVRR156 pKa = 11.84 WPSWSLGYY164 pKa = 9.67 PFQGYY169 pKa = 9.29 QGLGCRR175 pKa = 11.84 LSDD178 pKa = 3.4 GRR180 pKa = 11.84 LILSRR185 pKa = 11.84 PSEE188 pKa = 4.05 FSAIFQRR195 pKa = 11.84 WASRR199 pKa = 11.84 LHH201 pKa = 5.58 FWSGSSRR208 pKa = 11.84 GRR210 pKa = 11.84 RR211 pKa = 11.84 FRR213 pKa = 11.84 SSLWVVTNHH222 pKa = 5.85 LQTVLKK228 pKa = 10.71 HH229 pKa = 5.53 SGPYY233 pKa = 10.23 GLALYY238 pKa = 10.25 LKK240 pKa = 9.8 ASYY243 pKa = 10.66 NIVLRR248 pKa = 11.84 YY249 pKa = 9.6 VAGQPFEE256 pKa = 4.58 HH257 pKa = 7.22 AFTQCGVPVQLQNGLPKK274 pKa = 10.4 FLPRR278 pKa = 11.84 EE279 pKa = 3.69 WRR281 pKa = 11.84 VAIRR285 pKa = 11.84 SGSPRR290 pKa = 11.84 LLRR293 pKa = 11.84 IVLSLLYY300 pKa = 10.18 SYY302 pKa = 11.16 KK303 pKa = 10.53 GFKK306 pKa = 8.19 ATKK309 pKa = 8.28 PKK311 pKa = 10.09 PVAEE315 pKa = 3.9 IFKK318 pKa = 10.25 NISLAAHH325 pKa = 5.9 QPDD328 pKa = 5.36 DD329 pKa = 4.43 IIAFSKK335 pKa = 10.84 FCFEE339 pKa = 4.04 FLKK342 pKa = 11.0 NRR344 pKa = 11.84 ISPISEE350 pKa = 4.05 GEE352 pKa = 4.17 LNPPSLPASLKK363 pKa = 10.54 AGPNGPLSALAYY375 pKa = 10.34 GADD378 pKa = 3.43 AYY380 pKa = 10.9 AWIQRR385 pKa = 11.84 EE386 pKa = 4.13 SAPYY390 pKa = 8.84 QPSVPGPLEE399 pKa = 4.03 WVQHH403 pKa = 5.8 FGLDD407 pKa = 2.98 KK408 pKa = 10.24 WEE410 pKa = 4.29 KK411 pKa = 10.44 KK412 pKa = 9.31 FRR414 pKa = 11.84 AYY416 pKa = 10.95 SDD418 pKa = 3.15 WFLKK422 pKa = 10.62 RR423 pKa = 11.84 GFNHH427 pKa = 5.57 QFNVTFPPPTRR438 pKa = 11.84 KK439 pKa = 10.17 GFGVLKK445 pKa = 10.54 LKK447 pKa = 10.41 IALGLNVPEE456 pKa = 4.78 SKK458 pKa = 10.72 SIALNPKK465 pKa = 8.92 FAFHH469 pKa = 7.04 RR470 pKa = 11.84 RR471 pKa = 11.84 TVSYY475 pKa = 10.0 TPTRR479 pKa = 11.84 VEE481 pKa = 4.24 DD482 pKa = 4.27 VEE484 pKa = 5.01 ALQCLGLLLPVGRR497 pKa = 11.84 VHH499 pKa = 6.76 VLWEE503 pKa = 4.0 AAGKK507 pKa = 7.82 TRR509 pKa = 11.84 VIAMMDD515 pKa = 3.94 GLRR518 pKa = 11.84 QALLRR523 pKa = 11.84 PVHH526 pKa = 6.15 LAMFRR531 pKa = 11.84 RR532 pKa = 11.84 IDD534 pKa = 3.83 KK535 pKa = 10.84 IFGFCSGLYY544 pKa = 9.18 DD545 pKa = 3.08 QSGAVRR551 pKa = 11.84 SFAALNNRR559 pKa = 11.84 EE560 pKa = 4.09 VFSYY564 pKa = 10.47 DD565 pKa = 2.51 ISAATDD571 pKa = 3.86 TIPYY575 pKa = 9.76 QLYY578 pKa = 8.91 FPLMEE583 pKa = 4.55 FLLGKK588 pKa = 8.87 TGAVLWNHH596 pKa = 5.95 MVRR599 pKa = 11.84 GHH601 pKa = 6.33 PFIHH605 pKa = 6.93 LEE607 pKa = 4.33 GGDD610 pKa = 3.92 EE611 pKa = 3.95 PWAQRR616 pKa = 11.84 LAGSSSYY623 pKa = 10.82 YY624 pKa = 9.26 YY625 pKa = 10.61 EE626 pKa = 4.96 RR627 pKa = 11.84 GQPMGGYY634 pKa = 9.96 SSFAALDD641 pKa = 3.84 MLHH644 pKa = 6.74 HH645 pKa = 7.09 LIVQYY650 pKa = 10.96 SAFEE654 pKa = 4.12 AGYY657 pKa = 8.37 DD658 pKa = 3.78 TLNKK662 pKa = 9.88 TFDD665 pKa = 3.26 NYY667 pKa = 10.83 RR668 pKa = 11.84 ILGDD672 pKa = 3.74 DD673 pKa = 3.63 VVIGDD678 pKa = 3.48 VRR680 pKa = 11.84 VAKK683 pKa = 9.91 TYY685 pKa = 11.11 LNFMRR690 pKa = 11.84 TWNIPISEE698 pKa = 4.25 NKK700 pKa = 10.33 SLVSSKK706 pKa = 11.01 GVFQFLSEE714 pKa = 4.01 VFRR717 pKa = 11.84 GGEE720 pKa = 4.15 CLSPLSFRR728 pKa = 11.84 ADD730 pKa = 3.64 YY731 pKa = 10.43 QSKK734 pKa = 8.02 TFLEE738 pKa = 4.41 RR739 pKa = 11.84 FSFAATAVSRR749 pKa = 11.84 GWSSGLAMEE758 pKa = 5.02 GFGRR762 pKa = 11.84 LILSKK767 pKa = 10.89 DD768 pKa = 3.27 QFLSEE773 pKa = 3.22 IHH775 pKa = 7.07 RR776 pKa = 11.84 YY777 pKa = 8.66 RR778 pKa = 11.84 NRR780 pKa = 11.84 RR781 pKa = 11.84 PSAFIYY787 pKa = 10.59 SLLFALVTFAGEE799 pKa = 4.18 AGWALGSWLHH809 pKa = 5.92 NVVAHH814 pKa = 7.25 DD815 pKa = 4.96 PFPQLLSNKK824 pKa = 7.69 EE825 pKa = 4.09 LEE827 pKa = 4.48 SVEE830 pKa = 4.22 SKK832 pKa = 11.2 NFVFTILRR840 pKa = 11.84 QILFTEE846 pKa = 4.72 AGRR849 pKa = 11.84 IHH851 pKa = 6.98 EE852 pKa = 4.19 LQKK855 pKa = 11.11 AEE857 pKa = 4.16 SGVNQTRR864 pKa = 11.84 QFLDD868 pKa = 3.71 FGSIEE873 pKa = 3.91 FHH875 pKa = 6.95 NDD877 pKa = 2.19 IEE879 pKa = 4.72 NDD881 pKa = 3.43 VDD883 pKa = 4.9 SLWLLARR890 pKa = 11.84 EE891 pKa = 4.43 GKK893 pKa = 8.11 TLLEE897 pKa = 4.55 SADD900 pKa = 3.74 VTPDD904 pKa = 2.7 WVTSAFSYY912 pKa = 9.98 LRR914 pKa = 11.84 KK915 pKa = 9.97 VSGIIPRR922 pKa = 11.84 LSATGVVHH930 pKa = 6.72 PLDD933 pKa = 3.88 YY934 pKa = 10.98 LVLMKK939 pKa = 8.87 TTEE942 pKa = 3.97 DD943 pKa = 3.29 RR944 pKa = 11.84 NNIHH948 pKa = 7.14 KK949 pKa = 10.22 VNQWSPTNHH958 pKa = 6.65 DD959 pKa = 3.27 AEE961 pKa = 4.62 EE962 pKa = 4.07 IFRR965 pKa = 11.84 VRR967 pKa = 11.84 MVDD970 pKa = 3.26 TTFSLIKK977 pKa = 10.4 SNPYY981 pKa = 8.01 YY982 pKa = 10.8 HH983 pKa = 7.13 EE984 pKa = 4.31 VLTGISSPYY993 pKa = 10.21 GLALRR998 pKa = 11.84 PVPEE1002 pKa = 4.27 EE1003 pKa = 3.89 GRR1005 pKa = 11.84 PSGSSS1010 pKa = 2.91
Molecular weight: 113.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.819
IPC2_protein 8.873
IPC_protein 8.799
Toseland 9.341
ProMoST 9.224
Dawson 9.677
Bjellqvist 9.443
Wikipedia 9.882
Rodwell 9.853
Grimsley 9.78
Solomon 9.706
Lehninger 9.648
Nozaki 9.443
DTASelect 9.414
Thurlkill 9.502
EMBOSS 9.809
Sillero 9.619
Patrickios 4.762
IPC_peptide 9.692
IPC2_peptide 8.083
IPC2.peptide.svr19 7.779
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
1010
1010
1010
1010.0
113.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.525 ± 0.0
0.693 ± 0.0
3.465 ± 0.0
5.248 ± 0.0
6.04 ± 0.0
7.129 ± 0.0
2.475 ± 0.0
4.653 ± 0.0
4.554 ± 0.0
12.079 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.485 ± 0.0
3.861 ± 0.0
5.743 ± 0.0
3.267 ± 0.0
6.535 ± 0.0
9.109 ± 0.0
4.059 ± 0.0
6.436 ± 0.0
2.079 ± 0.0
3.564 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here