Amino acid dipepetide frequency for Enterococcus phage phiNASRA1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.409AlaAla: 0.409 ± 0.196
0.246AlaCys: 0.246 ± 0.141
2.783AlaAsp: 2.783 ± 0.545
3.929AlaGlu: 3.929 ± 0.603
2.538AlaPhe: 2.538 ± 0.621
2.538AlaGly: 2.538 ± 0.429
1.064AlaHis: 1.064 ± 0.302
4.502AlaIle: 4.502 ± 0.837
5.485AlaLys: 5.485 ± 0.692
5.157AlaLeu: 5.157 ± 0.876
2.783AlaMet: 2.783 ± 0.589
3.602AlaAsn: 3.602 ± 0.588
1.883AlaPro: 1.883 ± 0.336
1.883AlaGln: 1.883 ± 0.377
1.883AlaArg: 1.883 ± 0.401
3.029AlaSer: 3.029 ± 0.507
3.929AlaThr: 3.929 ± 0.633
4.42AlaVal: 4.42 ± 0.576
0.491AlaTrp: 0.491 ± 0.197
2.374AlaTyr: 2.374 ± 0.45
0.0AlaXaa: 0.0 ± 0.0
Cys
0.409CysAla: 0.409 ± 0.166
0.082CysCys: 0.082 ± 0.093
0.409CysAsp: 0.409 ± 0.204
0.737CysGlu: 0.737 ± 0.305
0.164CysPhe: 0.164 ± 0.127
0.409CysGly: 0.409 ± 0.184
0.246CysHis: 0.246 ± 0.156
0.409CysIle: 0.409 ± 0.203
0.573CysLys: 0.573 ± 0.219
0.573CysLeu: 0.573 ± 0.237
0.082CysMet: 0.082 ± 0.073
0.409CysAsn: 0.409 ± 0.223
0.0CysPro: 0.0 ± 0.0
0.082CysGln: 0.082 ± 0.076
0.246CysArg: 0.246 ± 0.136
0.409CysSer: 0.409 ± 0.187
0.327CysThr: 0.327 ± 0.191
0.246CysVal: 0.246 ± 0.142
0.164CysTrp: 0.164 ± 0.109
0.246CysTyr: 0.246 ± 0.145
0.0CysXaa: 0.0 ± 0.0
Asp
2.865AspAla: 2.865 ± 0.633
0.327AspCys: 0.327 ± 0.163
2.456AspAsp: 2.456 ± 0.475
4.257AspGlu: 4.257 ± 0.569
3.193AspPhe: 3.193 ± 0.559
4.912AspGly: 4.912 ± 0.638
0.655AspHis: 0.655 ± 0.264
4.748AspIle: 4.748 ± 0.7
5.812AspLys: 5.812 ± 0.605
5.157AspLeu: 5.157 ± 0.686
1.965AspMet: 1.965 ± 0.379
4.584AspAsn: 4.584 ± 0.731
1.637AspPro: 1.637 ± 0.516
1.31AspGln: 1.31 ± 0.31
1.637AspArg: 1.637 ± 0.314
2.783AspSer: 2.783 ± 0.453
3.602AspThr: 3.602 ± 0.611
3.929AspVal: 3.929 ± 0.582
0.737AspTrp: 0.737 ± 0.227
2.538AspTyr: 2.538 ± 0.438
0.0AspXaa: 0.0 ± 0.0
Glu
4.83GluAla: 4.83 ± 0.749
0.409GluCys: 0.409 ± 0.162
4.175GluAsp: 4.175 ± 0.848
6.713GluGlu: 6.713 ± 1.481
3.438GluPhe: 3.438 ± 0.541
5.157GluGly: 5.157 ± 0.626
1.555GluHis: 1.555 ± 0.367
3.111GluIle: 3.111 ± 0.493
6.467GluLys: 6.467 ± 0.785
7.777GluLeu: 7.777 ± 1.008
2.374GluMet: 2.374 ± 0.451
3.847GluAsn: 3.847 ± 0.529
2.538GluPro: 2.538 ± 0.558
3.438GluGln: 3.438 ± 0.612
3.029GluArg: 3.029 ± 0.595
3.274GluSer: 3.274 ± 0.503
3.52GluThr: 3.52 ± 0.47
5.894GluVal: 5.894 ± 0.859
2.046GluTrp: 2.046 ± 0.4
3.356GluTyr: 3.356 ± 0.749
0.0GluXaa: 0.0 ± 0.0
Phe
1.392PheAla: 1.392 ± 0.252
0.327PheCys: 0.327 ± 0.188
2.783PheAsp: 2.783 ± 0.482
3.111PheGlu: 3.111 ± 0.495
1.064PhePhe: 1.064 ± 0.359
2.783PheGly: 2.783 ± 0.601
0.246PheHis: 0.246 ± 0.155
4.666PheIle: 4.666 ± 0.614
4.748PheLys: 4.748 ± 0.624
2.128PheLeu: 2.128 ± 0.288
1.31PheMet: 1.31 ± 0.3
3.438PheAsn: 3.438 ± 0.538
0.491PhePro: 0.491 ± 0.217
1.637PheGln: 1.637 ± 0.3
1.719PheArg: 1.719 ± 0.424
2.865PheSer: 2.865 ± 0.547
3.274PheThr: 3.274 ± 0.622
2.538PheVal: 2.538 ± 0.468
0.491PheTrp: 0.491 ± 0.174
1.064PheTyr: 1.064 ± 0.281
0.0PheXaa: 0.0 ± 0.0
Gly
4.339GlyAla: 4.339 ± 1.035
0.409GlyCys: 0.409 ± 0.208
2.947GlyAsp: 2.947 ± 0.412
3.193GlyGlu: 3.193 ± 0.404
3.684GlyPhe: 3.684 ± 0.561
4.748GlyGly: 4.748 ± 1.392
0.819GlyHis: 0.819 ± 0.236
5.403GlyIle: 5.403 ± 0.801
6.385GlyLys: 6.385 ± 0.781
6.713GlyLeu: 6.713 ± 1.313
1.883GlyMet: 1.883 ± 0.371
4.666GlyAsn: 4.666 ± 0.715
0.9GlyPro: 0.9 ± 0.279
1.637GlyGln: 1.637 ± 0.307
2.947GlyArg: 2.947 ± 0.482
3.847GlySer: 3.847 ± 0.803
4.42GlyThr: 4.42 ± 0.832
4.093GlyVal: 4.093 ± 0.761
1.064GlyTrp: 1.064 ± 0.249
3.193GlyTyr: 3.193 ± 0.574
0.0GlyXaa: 0.0 ± 0.0
His
0.737HisAla: 0.737 ± 0.24
0.164HisCys: 0.164 ± 0.111
0.9HisAsp: 0.9 ± 0.276
0.9HisGlu: 0.9 ± 0.297
1.146HisPhe: 1.146 ± 0.355
1.228HisGly: 1.228 ± 0.451
0.246HisHis: 0.246 ± 0.145
1.31HisIle: 1.31 ± 0.33
1.719HisLys: 1.719 ± 0.451
1.392HisLeu: 1.392 ± 0.408
0.573HisMet: 0.573 ± 0.254
1.228HisAsn: 1.228 ± 0.363
0.409HisPro: 0.409 ± 0.2
0.819HisGln: 0.819 ± 0.236
1.228HisArg: 1.228 ± 0.385
0.573HisSer: 0.573 ± 0.225
0.982HisThr: 0.982 ± 0.359
0.819HisVal: 0.819 ± 0.251
0.082HisTrp: 0.082 ± 0.078
1.228HisTyr: 1.228 ± 0.307
0.0HisXaa: 0.0 ± 0.0
Ile
3.602IleAla: 3.602 ± 0.553
0.737IleCys: 0.737 ± 0.261
5.485IleAsp: 5.485 ± 0.61
5.894IleGlu: 5.894 ± 0.951
2.783IlePhe: 2.783 ± 0.57
4.339IleGly: 4.339 ± 0.699
1.064IleHis: 1.064 ± 0.357
3.438IleIle: 3.438 ± 0.489
6.221IleLys: 6.221 ± 0.67
4.339IleLeu: 4.339 ± 0.621
1.883IleMet: 1.883 ± 0.442
4.666IleAsn: 4.666 ± 0.699
2.947IlePro: 2.947 ± 0.421
3.111IleGln: 3.111 ± 0.49
2.21IleArg: 2.21 ± 0.421
4.257IleSer: 4.257 ± 0.513
4.42IleThr: 4.42 ± 0.584
3.929IleVal: 3.929 ± 0.414
0.655IleTrp: 0.655 ± 0.209
1.31IleTyr: 1.31 ± 0.31
0.0IleXaa: 0.0 ± 0.0
Lys
6.631LysAla: 6.631 ± 0.956
0.655LysCys: 0.655 ± 0.318
5.566LysAsp: 5.566 ± 0.651
8.432LysGlu: 8.432 ± 1.01
3.111LysPhe: 3.111 ± 0.392
5.566LysGly: 5.566 ± 0.846
1.719LysHis: 1.719 ± 0.443
4.666LysIle: 4.666 ± 0.71
6.467LysLys: 6.467 ± 0.672
6.794LysLeu: 6.794 ± 1.068
3.111LysMet: 3.111 ± 0.378
5.321LysAsn: 5.321 ± 0.445
4.093LysPro: 4.093 ± 0.653
3.929LysGln: 3.929 ± 0.561
3.602LysArg: 3.602 ± 0.529
4.502LysSer: 4.502 ± 0.968
5.648LysThr: 5.648 ± 0.568
6.467LysVal: 6.467 ± 0.625
1.064LysTrp: 1.064 ± 0.397
3.847LysTyr: 3.847 ± 0.559
0.0LysXaa: 0.0 ± 0.0
Leu
3.684LeuAla: 3.684 ± 0.635
0.327LeuCys: 0.327 ± 0.176
6.058LeuAsp: 6.058 ± 0.654
8.022LeuGlu: 8.022 ± 0.843
2.62LeuPhe: 2.62 ± 0.449
5.894LeuGly: 5.894 ± 1.08
0.982LeuHis: 0.982 ± 0.286
4.502LeuIle: 4.502 ± 0.699
7.777LeuLys: 7.777 ± 0.803
6.713LeuLeu: 6.713 ± 0.858
1.801LeuMet: 1.801 ± 0.345
5.321LeuAsn: 5.321 ± 0.531
2.783LeuPro: 2.783 ± 0.509
4.011LeuGln: 4.011 ± 0.515
2.538LeuArg: 2.538 ± 0.481
4.502LeuSer: 4.502 ± 0.666
4.339LeuThr: 4.339 ± 0.554
5.403LeuVal: 5.403 ± 0.775
1.064LeuTrp: 1.064 ± 0.312
2.947LeuTyr: 2.947 ± 0.658
0.0LeuXaa: 0.0 ± 0.0
Met
1.473MetAla: 1.473 ± 0.395
0.164MetCys: 0.164 ± 0.107
1.31MetAsp: 1.31 ± 0.398
1.31MetGlu: 1.31 ± 0.4
1.31MetPhe: 1.31 ± 0.408
2.374MetGly: 2.374 ± 0.465
0.246MetHis: 0.246 ± 0.132
2.21MetIle: 2.21 ± 0.417
2.62MetLys: 2.62 ± 0.481
1.965MetLeu: 1.965 ± 0.462
0.491MetMet: 0.491 ± 0.18
2.21MetAsn: 2.21 ± 0.36
0.491MetPro: 0.491 ± 0.213
0.819MetGln: 0.819 ± 0.213
1.965MetArg: 1.965 ± 0.442
2.046MetSer: 2.046 ± 0.428
1.719MetThr: 1.719 ± 0.335
1.883MetVal: 1.883 ± 0.454
0.9MetTrp: 0.9 ± 0.368
1.473MetTyr: 1.473 ± 0.354
0.0MetXaa: 0.0 ± 0.0
Asn
3.847AsnAla: 3.847 ± 0.814
0.082AsnCys: 0.082 ± 0.082
2.783AsnAsp: 2.783 ± 0.59
5.894AsnGlu: 5.894 ± 0.678
1.555AsnPhe: 1.555 ± 0.371
6.549AsnGly: 6.549 ± 0.64
1.392AsnHis: 1.392 ± 0.446
4.83AsnIle: 4.83 ± 0.628
6.058AsnLys: 6.058 ± 0.704
5.321AsnLeu: 5.321 ± 0.556
2.292AsnMet: 2.292 ± 0.372
4.093AsnAsn: 4.093 ± 0.584
2.046AsnPro: 2.046 ± 0.482
1.637AsnGln: 1.637 ± 0.324
1.392AsnArg: 1.392 ± 0.238
3.929AsnSer: 3.929 ± 0.533
4.748AsnThr: 4.748 ± 0.825
3.52AsnVal: 3.52 ± 0.522
0.819AsnTrp: 0.819 ± 0.299
3.52AsnTyr: 3.52 ± 0.763
0.0AsnXaa: 0.0 ± 0.0
Pro
1.637ProAla: 1.637 ± 0.377
0.164ProCys: 0.164 ± 0.121
2.374ProAsp: 2.374 ± 0.533
3.438ProGlu: 3.438 ± 0.601
1.31ProPhe: 1.31 ± 0.291
0.246ProGly: 0.246 ± 0.113
0.164ProHis: 0.164 ± 0.132
1.883ProIle: 1.883 ± 0.295
2.62ProLys: 2.62 ± 0.645
2.783ProLeu: 2.783 ± 0.549
0.9ProMet: 0.9 ± 0.231
2.046ProAsn: 2.046 ± 0.405
0.246ProPro: 0.246 ± 0.137
1.228ProGln: 1.228 ± 0.353
0.737ProArg: 0.737 ± 0.234
1.637ProSer: 1.637 ± 0.351
2.538ProThr: 2.538 ± 0.458
1.801ProVal: 1.801 ± 0.416
0.164ProTrp: 0.164 ± 0.107
1.555ProTyr: 1.555 ± 0.455
0.0ProXaa: 0.0 ± 0.0
Gln
2.538GlnAla: 2.538 ± 0.367
0.491GlnCys: 0.491 ± 0.243
1.555GlnAsp: 1.555 ± 0.285
2.456GlnGlu: 2.456 ± 0.46
1.719GlnPhe: 1.719 ± 0.398
1.801GlnGly: 1.801 ± 0.286
0.655GlnHis: 0.655 ± 0.228
3.193GlnIle: 3.193 ± 0.461
2.456GlnLys: 2.456 ± 0.456
3.438GlnLeu: 3.438 ± 0.524
0.819GlnMet: 0.819 ± 0.22
1.473GlnAsn: 1.473 ± 0.303
1.31GlnPro: 1.31 ± 0.364
1.965GlnGln: 1.965 ± 0.447
2.046GlnArg: 2.046 ± 0.393
2.292GlnSer: 2.292 ± 0.512
1.883GlnThr: 1.883 ± 0.321
2.538GlnVal: 2.538 ± 0.455
0.327GlnTrp: 0.327 ± 0.149
2.128GlnTyr: 2.128 ± 0.404
0.0GlnXaa: 0.0 ± 0.0
Arg
1.801ArgAla: 1.801 ± 0.399
0.246ArgCys: 0.246 ± 0.133
2.128ArgAsp: 2.128 ± 0.455
1.965ArgGlu: 1.965 ± 0.446
1.719ArgPhe: 1.719 ± 0.344
1.965ArgGly: 1.965 ± 0.622
0.982ArgHis: 0.982 ± 0.308
2.62ArgIle: 2.62 ± 0.547
3.438ArgLys: 3.438 ± 0.616
2.947ArgLeu: 2.947 ± 0.562
1.064ArgMet: 1.064 ± 0.287
2.21ArgAsn: 2.21 ± 0.445
1.31ArgPro: 1.31 ± 0.313
1.637ArgGln: 1.637 ± 0.369
1.31ArgArg: 1.31 ± 0.272
2.046ArgSer: 2.046 ± 0.337
2.046ArgThr: 2.046 ± 0.343
2.456ArgVal: 2.456 ± 0.43
0.491ArgTrp: 0.491 ± 0.314
1.31ArgTyr: 1.31 ± 0.346
0.0ArgXaa: 0.0 ± 0.0
Ser
3.029SerAla: 3.029 ± 0.665
0.164SerCys: 0.164 ± 0.118
2.62SerAsp: 2.62 ± 0.364
3.602SerGlu: 3.602 ± 0.517
2.374SerPhe: 2.374 ± 0.397
5.075SerGly: 5.075 ± 0.715
2.128SerHis: 2.128 ± 0.406
3.111SerIle: 3.111 ± 0.491
5.157SerLys: 5.157 ± 0.656
4.42SerLeu: 4.42 ± 0.597
1.146SerMet: 1.146 ± 0.278
4.666SerAsn: 4.666 ± 0.531
0.655SerPro: 0.655 ± 0.219
1.883SerGln: 1.883 ± 0.497
1.228SerArg: 1.228 ± 0.286
3.274SerSer: 3.274 ± 0.737
4.502SerThr: 4.502 ± 0.697
3.193SerVal: 3.193 ± 0.597
0.819SerTrp: 0.819 ± 0.284
2.128SerTyr: 2.128 ± 0.542
0.0SerXaa: 0.0 ± 0.0
Thr
3.684ThrAla: 3.684 ± 0.636
0.082ThrCys: 0.082 ± 0.073
3.766ThrAsp: 3.766 ± 0.583
3.847ThrGlu: 3.847 ± 0.503
2.456ThrPhe: 2.456 ± 0.464
4.584ThrGly: 4.584 ± 0.559
1.801ThrHis: 1.801 ± 0.413
5.239ThrIle: 5.239 ± 0.668
6.713ThrLys: 6.713 ± 0.805
5.894ThrLeu: 5.894 ± 0.82
1.473ThrMet: 1.473 ± 0.397
3.929ThrAsn: 3.929 ± 0.608
2.456ThrPro: 2.456 ± 0.429
2.21ThrGln: 2.21 ± 0.346
2.128ThrArg: 2.128 ± 0.4
2.21ThrSer: 2.21 ± 0.524
4.011ThrThr: 4.011 ± 0.542
4.093ThrVal: 4.093 ± 0.465
0.737ThrTrp: 0.737 ± 0.289
1.801ThrTyr: 1.801 ± 0.446
0.0ThrXaa: 0.0 ± 0.0
Val
5.403ValAla: 5.403 ± 0.779
0.327ValCys: 0.327 ± 0.153
5.239ValAsp: 5.239 ± 0.638
4.339ValGlu: 4.339 ± 0.535
3.274ValPhe: 3.274 ± 0.542
4.175ValGly: 4.175 ± 0.724
0.9ValHis: 0.9 ± 0.304
3.52ValIle: 3.52 ± 0.446
5.648ValLys: 5.648 ± 0.807
3.847ValLeu: 3.847 ± 0.468
2.046ValMet: 2.046 ± 0.281
4.339ValAsn: 4.339 ± 0.548
1.965ValPro: 1.965 ± 0.398
2.292ValGln: 2.292 ± 0.432
1.965ValArg: 1.965 ± 0.425
5.157ValSer: 5.157 ± 0.597
3.929ValThr: 3.929 ± 0.547
5.075ValVal: 5.075 ± 0.555
0.655ValTrp: 0.655 ± 0.213
2.456ValTyr: 2.456 ± 0.472
0.0ValXaa: 0.0 ± 0.0
Trp
0.491TrpAla: 0.491 ± 0.204
0.327TrpCys: 0.327 ± 0.154
0.819TrpAsp: 0.819 ± 0.274
1.392TrpGlu: 1.392 ± 0.252
0.819TrpPhe: 0.819 ± 0.24
1.064TrpGly: 1.064 ± 0.31
0.164TrpHis: 0.164 ± 0.112
0.573TrpIle: 0.573 ± 0.2
0.819TrpLys: 0.819 ± 0.252
0.982TrpLeu: 0.982 ± 0.294
0.164TrpMet: 0.164 ± 0.107
0.655TrpAsn: 0.655 ± 0.198
0.0TrpPro: 0.0 ± 0.0
0.573TrpGln: 0.573 ± 0.168
0.819TrpArg: 0.819 ± 0.32
0.491TrpSer: 0.491 ± 0.184
0.819TrpThr: 0.819 ± 0.254
1.637TrpVal: 1.637 ± 0.274
0.246TrpTrp: 0.246 ± 0.118
0.246TrpTyr: 0.246 ± 0.167
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.128TyrAla: 2.128 ± 0.428
0.491TyrCys: 0.491 ± 0.238
2.947TyrAsp: 2.947 ± 0.533
3.52TyrGlu: 3.52 ± 0.599
1.801TyrPhe: 1.801 ± 0.377
1.965TyrGly: 1.965 ± 0.342
0.655TyrHis: 0.655 ± 0.222
3.356TyrIle: 3.356 ± 0.639
4.011TyrLys: 4.011 ± 0.674
2.947TyrLeu: 2.947 ± 0.556
0.737TyrMet: 0.737 ± 0.255
3.602TyrAsn: 3.602 ± 0.625
1.31TyrPro: 1.31 ± 0.393
0.982TyrGln: 0.982 ± 0.256
1.064TyrArg: 1.064 ± 0.321
2.046TyrSer: 2.046 ± 0.498
2.456TyrThr: 2.456 ± 0.554
2.62TyrVal: 2.62 ± 0.702
0.082TyrTrp: 0.082 ± 0.075
1.801TyrTyr: 1.801 ± 0.487
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 62 proteins (12217 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski