Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis)
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2545 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8R877|Q8R877_CALS4 ABC_transp_aux domain-containing protein OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=TTE2143 PE=4 SV=1
MM1 pKa = 7.61 EE2 pKa = 5.14 KK3 pKa = 9.49 WQCTVCGYY11 pKa = 10.3 IYY13 pKa = 10.46 DD14 pKa = 4.2 PEE16 pKa = 6.17 KK17 pKa = 10.88 GDD19 pKa = 3.84 PSQGIPPGTPFEE31 pKa = 4.45 EE32 pKa = 5.71 LPDD35 pKa = 3.91 DD36 pKa = 4.55 WVCPDD41 pKa = 4.2 CGAGKK46 pKa = 10.22 DD47 pKa = 3.98 LFEE50 pKa = 5.36 KK51 pKa = 10.13 MM52 pKa = 4.33
Molecular weight: 5.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.8
IPC2_protein 3.999
IPC_protein 3.872
Toseland 3.681
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.706
Grimsley 3.592
Solomon 3.834
Lehninger 3.783
Nozaki 3.999
DTASelect 4.164
Thurlkill 3.745
EMBOSS 3.808
Sillero 3.986
Patrickios 0.693
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.9
Protein with the highest isoelectric point:
>sp|Q8R6L9|MSCS_CALS4 Small-conductance mechanosensitive channel OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=mscS PE=1 SV=1
MM1 pKa = 7.45 LRR3 pKa = 11.84 TYY5 pKa = 9.82 QPKK8 pKa = 9.4 KK9 pKa = 7.87 RR10 pKa = 11.84 HH11 pKa = 5.46 RR12 pKa = 11.84 KK13 pKa = 8.28 KK14 pKa = 9.71 VHH16 pKa = 5.38 GFRR19 pKa = 11.84 KK20 pKa = 9.99 RR21 pKa = 11.84 MSTKK25 pKa = 10.21 AGRR28 pKa = 11.84 NVLKK32 pKa = 10.49 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.48 GRR39 pKa = 11.84 HH40 pKa = 5.75 RR41 pKa = 11.84 LTAA44 pKa = 4.06
Molecular weight: 5.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.434
IPC2_protein 11.14
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.427
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.149
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.052
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2545
0
2545
762176
37
2862
299.5
33.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.528 ± 0.044
0.761 ± 0.017
4.948 ± 0.035
8.371 ± 0.065
4.401 ± 0.042
6.872 ± 0.046
1.43 ± 0.018
8.961 ± 0.05
8.784 ± 0.052
9.549 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.544 ± 0.02
4.35 ± 0.035
3.577 ± 0.031
2.336 ± 0.025
4.32 ± 0.038
5.273 ± 0.036
4.62 ± 0.035
7.566 ± 0.041
0.788 ± 0.017
4.022 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here