Halobiforma lacisalsi AJ5

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Natrialbales; Natrialbaceae; Halobiforma; Halobiforma lacisalsi

Average proteome isoelectric point is 4.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4151 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M0L9E0|M0L9E0_9EURY Transcription regulator AsnC-type-like protein OS=Halobiforma lacisalsi AJ5 OX=358396 GN=C445_16649 PE=4 SV=1
MM1 pKa = 7.71PPDD4 pKa = 4.77DD5 pKa = 5.18SPTSDD10 pKa = 2.82RR11 pKa = 11.84RR12 pKa = 11.84SVLKK16 pKa = 10.69AAGALGAFLGSSGVAAADD34 pKa = 3.75GDD36 pKa = 4.28DD37 pKa = 4.05EE38 pKa = 6.67ADD40 pKa = 3.45AGATEE45 pKa = 4.47LLVGVSPDD53 pKa = 3.31VSDD56 pKa = 3.87VEE58 pKa = 4.6SAVDD62 pKa = 3.45SSIVDD67 pKa = 3.63GQVVHH72 pKa = 6.2TNEE75 pKa = 4.84AIHH78 pKa = 6.05YY79 pKa = 7.12ATIEE83 pKa = 4.17FPEE86 pKa = 4.15NTPEE90 pKa = 3.87EE91 pKa = 4.31AKK93 pKa = 9.87TAIEE97 pKa = 4.28DD98 pKa = 3.57ALASVDD104 pKa = 3.57EE105 pKa = 4.29IEE107 pKa = 4.39YY108 pKa = 10.4VEE110 pKa = 4.34EE111 pKa = 3.95NATIEE116 pKa = 4.62SFAAPNDD123 pKa = 3.91PYY125 pKa = 11.03YY126 pKa = 10.98GSQHH130 pKa = 6.72APQQVNCEE138 pKa = 4.2GAWDD142 pKa = 3.89EE143 pKa = 4.46TFGDD147 pKa = 3.78SDD149 pKa = 3.93VTISIVDD156 pKa = 3.16TGTAYY161 pKa = 10.55DD162 pKa = 4.16HH163 pKa = 7.17EE164 pKa = 4.51NLAEE168 pKa = 4.18NVDD171 pKa = 3.72DD172 pKa = 5.52RR173 pKa = 11.84IGEE176 pKa = 4.24VFVGGGSDD184 pKa = 3.64PYY186 pKa = 10.21PRR188 pKa = 11.84SGSEE192 pKa = 3.54THH194 pKa = 5.47GTMVSGAAVGATDD207 pKa = 4.23NGTGHH212 pKa = 7.29AGVSNCSMLSARR224 pKa = 11.84ALDD227 pKa = 3.78ASGTGSLADD236 pKa = 3.82IADD239 pKa = 4.01AVQWSADD246 pKa = 3.37QGVDD250 pKa = 4.49VINLSLGSSSDD261 pKa = 2.68WHH263 pKa = 5.63TLRR266 pKa = 11.84NACQYY271 pKa = 11.39AYY273 pKa = 10.5DD274 pKa = 3.86QGCLLVAAAGNSGGSVAYY292 pKa = 8.75PAVYY296 pKa = 10.35DD297 pKa = 3.73SVIAVSALDD306 pKa = 3.39SHH308 pKa = 7.25DD309 pKa = 3.8RR310 pKa = 11.84LASFSNRR317 pKa = 11.84GSEE320 pKa = 4.35IEE322 pKa = 4.02LAAPGTSIVTTTLNDD337 pKa = 3.87GYY339 pKa = 9.23TRR341 pKa = 11.84ASGTSIASPIVAGVAGLVLSEE362 pKa = 4.31YY363 pKa = 10.13PDD365 pKa = 4.3LDD367 pKa = 3.69VEE369 pKa = 4.47TLRR372 pKa = 11.84KK373 pKa = 9.47HH374 pKa = 6.27LRR376 pKa = 11.84QTATDD381 pKa = 3.55VGLSAAAQGYY391 pKa = 9.48GRR393 pKa = 11.84VDD395 pKa = 2.94ADD397 pKa = 3.46AAVNTVPDD405 pKa = 4.51GYY407 pKa = 10.96EE408 pKa = 4.16PEE410 pKa = 4.24NPDD413 pKa = 4.08YY414 pKa = 11.61GDD416 pKa = 5.42DD417 pKa = 5.12DD418 pKa = 5.78DD419 pKa = 6.33EE420 pKa = 6.22EE421 pKa = 7.14DD422 pKa = 3.72DD423 pKa = 4.55TEE425 pKa = 5.66DD426 pKa = 4.01PGIDD430 pKa = 3.72EE431 pKa = 4.41VWNDD435 pKa = 3.46DD436 pKa = 3.54EE437 pKa = 5.22SNHH440 pKa = 5.8VEE442 pKa = 4.65LEE444 pKa = 4.12GEE446 pKa = 4.15SLDD449 pKa = 3.86RR450 pKa = 11.84VAEE453 pKa = 3.85YY454 pKa = 10.28RR455 pKa = 11.84IEE457 pKa = 4.23GRR459 pKa = 11.84GTAEE463 pKa = 3.98PGEE466 pKa = 4.29NANTDD471 pKa = 3.28PDD473 pKa = 3.93DD474 pKa = 4.41PYY476 pKa = 11.7RR477 pKa = 11.84DD478 pKa = 3.23IATTDD483 pKa = 3.16GEE485 pKa = 4.46EE486 pKa = 4.28FVVEE490 pKa = 5.18GYY492 pKa = 10.79LGGYY496 pKa = 9.47VDD498 pKa = 5.47DD499 pKa = 4.26FHH501 pKa = 6.95ITGAVTDD508 pKa = 3.79VEE510 pKa = 4.84TNVDD514 pKa = 3.48LTAVVNGHH522 pKa = 6.72AFDD525 pKa = 4.86LRR527 pKa = 11.84DD528 pKa = 3.64LEE530 pKa = 5.7GVGDD534 pKa = 4.1WEE536 pKa = 5.64ADD538 pKa = 3.6DD539 pKa = 6.33GEE541 pKa = 4.51PQCGDD546 pKa = 3.36EE547 pKa = 4.46TVTASAEE554 pKa = 4.2GSLSGRR560 pKa = 11.84WWGGTDD566 pKa = 2.9SYY568 pKa = 11.35TYY570 pKa = 10.59SLRR573 pKa = 11.84TADD576 pKa = 3.68PCSATVALDD585 pKa = 4.07GPDD588 pKa = 3.51GADD591 pKa = 3.09FDD593 pKa = 6.05LYY595 pKa = 10.21VTLDD599 pKa = 3.27GRR601 pKa = 11.84SPSRR605 pKa = 11.84WDD607 pKa = 3.27YY608 pKa = 11.83DD609 pKa = 3.64EE610 pKa = 6.18SSDD613 pKa = 4.58GSEE616 pKa = 3.85SDD618 pKa = 3.32EE619 pKa = 5.02SITVDD624 pKa = 4.21LEE626 pKa = 4.04GDD628 pKa = 3.47EE629 pKa = 4.27EE630 pKa = 5.04LRR632 pKa = 11.84LQVHH636 pKa = 6.76ANSGSGEE643 pKa = 4.14YY644 pKa = 10.25EE645 pKa = 3.88VTVEE649 pKa = 3.86EE650 pKa = 4.63RR651 pKa = 11.84GRR653 pKa = 3.57

Molecular weight: 68.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M0LTS9|M0LTS9_9EURY 2-keto-3-deoxygluconate kinase OS=Halobiforma lacisalsi AJ5 OX=358396 GN=C445_01956 PE=4 SV=1
MM1 pKa = 7.34AADD4 pKa = 4.16RR5 pKa = 11.84GYY7 pKa = 11.06DD8 pKa = 3.32DD9 pKa = 4.22KK10 pKa = 11.8AFRR13 pKa = 11.84DD14 pKa = 3.75EE15 pKa = 4.49LRR17 pKa = 11.84ANGIRR22 pKa = 11.84PLIKK26 pKa = 10.2HH27 pKa = 6.53RR28 pKa = 11.84IYY30 pKa = 9.95WALDD34 pKa = 3.19HH35 pKa = 6.59AHH37 pKa = 6.85NARR40 pKa = 11.84MDD42 pKa = 3.65RR43 pKa = 11.84DD44 pKa = 3.47RR45 pKa = 11.84YY46 pKa = 8.01NRR48 pKa = 11.84RR49 pKa = 11.84WMVEE53 pKa = 4.13TVFSSLKK60 pKa = 8.84RR61 pKa = 11.84TLGSAVRR68 pKa = 11.84ARR70 pKa = 11.84SWHH73 pKa = 5.61LEE75 pKa = 3.59FRR77 pKa = 11.84EE78 pKa = 4.1MVLKK82 pKa = 10.7CAVYY86 pKa = 9.85NLRR89 pKa = 11.84RR90 pKa = 11.84TVRR93 pKa = 11.84YY94 pKa = 8.29PP95 pKa = 2.95

Molecular weight: 11.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4151

0

4151

1212350

24

1709

292.1

31.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.605 ± 0.055

0.72 ± 0.013

8.783 ± 0.053

9.646 ± 0.062

3.21 ± 0.027

8.569 ± 0.039

1.91 ± 0.019

4.074 ± 0.028

1.65 ± 0.024

8.855 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.654 ± 0.016

2.177 ± 0.019

4.743 ± 0.027

2.219 ± 0.025

6.798 ± 0.04

5.415 ± 0.025

6.295 ± 0.032

8.881 ± 0.041

1.112 ± 0.016

2.681 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski