Porcine rotavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Rotavirus; Rotavirus C

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0K2SR53|A0A0K2SR53_9REOV NSP2 OS=Porcine rotavirus OX=10913 PE=4 SV=1
MM1 pKa = 7.57SDD3 pKa = 3.24VPRR6 pKa = 11.84FDD8 pKa = 4.66LRR10 pKa = 11.84SKK12 pKa = 10.93RR13 pKa = 11.84KK14 pKa = 5.54TQKK17 pKa = 9.38KK18 pKa = 8.25QKK20 pKa = 10.39VDD22 pKa = 3.28IFDD25 pKa = 4.36EE26 pKa = 4.48DD27 pKa = 4.13DD28 pKa = 3.99KK29 pKa = 11.62EE30 pKa = 5.11SSIQVDD36 pKa = 4.07GEE38 pKa = 4.3SDD40 pKa = 3.61SLASADD46 pKa = 3.44TSSNHH51 pKa = 6.35SYY53 pKa = 11.12EE54 pKa = 5.07DD55 pKa = 3.49YY56 pKa = 11.26SKK58 pKa = 11.08AYY60 pKa = 9.65KK61 pKa = 10.4DD62 pKa = 3.68LTLEE66 pKa = 4.42TPVEE70 pKa = 3.98QPIGDD75 pKa = 4.41DD76 pKa = 3.71DD77 pKa = 4.14QVDD80 pKa = 4.01SVSTILEE87 pKa = 4.3SKK89 pKa = 10.42VDD91 pKa = 3.59NSWYY95 pKa = 10.22DD96 pKa = 3.04KK97 pKa = 10.07TVEE100 pKa = 4.0VEE102 pKa = 4.13EE103 pKa = 4.78RR104 pKa = 11.84VQKK107 pKa = 9.76PKK109 pKa = 11.08RR110 pKa = 11.84MTKK113 pKa = 10.02QKK115 pKa = 10.54AQQIDD120 pKa = 4.28EE121 pKa = 4.42DD122 pKa = 4.24TSDD125 pKa = 5.23LKK127 pKa = 11.36LQLAQLSLRR136 pKa = 11.84IQKK139 pKa = 8.92MEE141 pKa = 3.84SEE143 pKa = 4.5TRR145 pKa = 11.84AKK147 pKa = 10.04TIDD150 pKa = 3.53SAYY153 pKa = 9.19HH154 pKa = 5.64TIITQAEE161 pKa = 4.42NLTTPQKK168 pKa = 10.61KK169 pKa = 10.11SLIAAIIATMRR180 pKa = 3.55

Molecular weight:
20.51 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0K2SRD6|A0A0K2SRD6_9REOV NSP3 OS=Porcine rotavirus OX=10913 PE=4 SV=1
MM1 pKa = 8.15DD2 pKa = 5.07GANYY6 pKa = 9.77LAWLARR12 pKa = 11.84DD13 pKa = 3.39IVRR16 pKa = 11.84NLSYY20 pKa = 10.23TALVYY25 pKa = 10.85NNPKK29 pKa = 9.62VAIVTLQDD37 pKa = 3.37SKK39 pKa = 11.87EE40 pKa = 3.91EE41 pKa = 4.22FISFEE46 pKa = 4.33KK47 pKa = 9.68EE48 pKa = 3.49QKK50 pKa = 9.31TPSEE54 pKa = 4.74LIAEE58 pKa = 4.24IQNIVEE64 pKa = 4.5SNLSIEE70 pKa = 4.82DD71 pKa = 4.07KK72 pKa = 10.92INNLLTIRR80 pKa = 11.84YY81 pKa = 8.36ISVYY85 pKa = 10.67VDD87 pKa = 3.43DD88 pKa = 5.87KK89 pKa = 10.99SDD91 pKa = 3.17KK92 pKa = 10.94RR93 pKa = 11.84EE94 pKa = 4.08IVSKK98 pKa = 10.9LLDD101 pKa = 4.29KK102 pKa = 10.69IIKK105 pKa = 8.25TLEE108 pKa = 4.02NKK110 pKa = 9.9VSISEE115 pKa = 4.23TLKK118 pKa = 10.92KK119 pKa = 10.81CIDD122 pKa = 4.62DD123 pKa = 3.62IRR125 pKa = 11.84NEE127 pKa = 4.01AKK129 pKa = 9.61SWRR132 pKa = 11.84IKK134 pKa = 10.64NSSSFKK140 pKa = 10.09NYY142 pKa = 9.99HH143 pKa = 5.38YY144 pKa = 10.73NQLVSDD150 pKa = 4.36FIKK153 pKa = 10.99YY154 pKa = 10.41NEE156 pKa = 3.92FEE158 pKa = 4.06ILEE161 pKa = 4.75DD162 pKa = 3.52EE163 pKa = 5.68DD164 pKa = 3.83EE165 pKa = 4.54YY166 pKa = 11.38RR167 pKa = 11.84WKK169 pKa = 10.91SDD171 pKa = 3.13TLQGLSPNYY180 pKa = 9.03NHH182 pKa = 6.95RR183 pKa = 11.84THH185 pKa = 6.5TLISSIIFAVQSRR198 pKa = 11.84MLRR201 pKa = 11.84YY202 pKa = 9.56NDD204 pKa = 3.43EE205 pKa = 4.06QLEE208 pKa = 4.3VLLFMFNKK216 pKa = 9.44IRR218 pKa = 11.84EE219 pKa = 4.49CYY221 pKa = 9.17NDD223 pKa = 4.35GYY225 pKa = 11.58LEE227 pKa = 4.31LLPNRR232 pKa = 11.84KK233 pKa = 8.54WSHH236 pKa = 5.4TIKK239 pKa = 10.85DD240 pKa = 3.3LRR242 pKa = 11.84TNKK245 pKa = 9.68KK246 pKa = 9.58IKK248 pKa = 9.6MYY250 pKa = 9.16SAKK253 pKa = 9.95IIHH256 pKa = 6.37ATCAMISILHH266 pKa = 7.08ADD268 pKa = 4.29PIDD271 pKa = 4.2PYY273 pKa = 11.25FLAQIVTTFEE283 pKa = 4.59EE284 pKa = 4.94IPAHH288 pKa = 6.13AAKK291 pKa = 10.27QLSSPMTLYY300 pKa = 10.45IGVAQIKK307 pKa = 9.84SNIVISTQRR316 pKa = 11.84AAEE319 pKa = 4.15SVASIKK325 pKa = 10.77PNVSRR330 pKa = 11.84LDD332 pKa = 3.64EE333 pKa = 4.35SQVAEE338 pKa = 4.29WNEE341 pKa = 3.78EE342 pKa = 3.39MDD344 pKa = 3.77EE345 pKa = 4.19YY346 pKa = 11.08HH347 pKa = 6.73FKK349 pKa = 11.17SSILVNMMKK358 pKa = 10.23TNIYY362 pKa = 9.64DD363 pKa = 3.85VPVHH367 pKa = 5.62TFYY370 pKa = 11.2KK371 pKa = 10.33IFNCFSATFHH381 pKa = 6.21TGHH384 pKa = 6.89RR385 pKa = 11.84VDD387 pKa = 4.38NPQDD391 pKa = 3.96AIEE394 pKa = 4.35AQVKK398 pKa = 9.4VEE400 pKa = 3.92YY401 pKa = 10.13TSDD404 pKa = 3.3INRR407 pKa = 11.84EE408 pKa = 4.04MYY410 pKa = 9.66DD411 pKa = 2.82QYY413 pKa = 11.92YY414 pKa = 10.45FLLKK418 pKa = 10.66RR419 pKa = 11.84MLTDD423 pKa = 3.07QFAEE427 pKa = 4.23YY428 pKa = 10.49AEE430 pKa = 4.3EE431 pKa = 4.15MFHH434 pKa = 7.21KK435 pKa = 10.7YY436 pKa = 10.57NSDD439 pKa = 3.12VTAEE443 pKa = 4.32SLASMSNSSNGYY455 pKa = 7.81SRR457 pKa = 11.84SVKK460 pKa = 10.62FIDD463 pKa = 5.01RR464 pKa = 11.84EE465 pKa = 4.01IKK467 pKa = 6.98TTKK470 pKa = 10.6KK471 pKa = 9.58MLHH474 pKa = 6.67LDD476 pKa = 3.88DD477 pKa = 6.17DD478 pKa = 4.61LSKK481 pKa = 11.35DD482 pKa = 4.18LDD484 pKa = 3.72FTNIGEE490 pKa = 4.39QISKK494 pKa = 9.63GIPMGTRR501 pKa = 11.84NVPARR506 pKa = 11.84QTRR509 pKa = 11.84GIFILNWQVAAIQHH523 pKa = 6.29TIAEE527 pKa = 4.27FLYY530 pKa = 10.6KK531 pKa = 9.96KK532 pKa = 10.11AKK534 pKa = 9.99KK535 pKa = 10.15GGFGATFAEE544 pKa = 4.84AYY546 pKa = 8.25VAKK549 pKa = 10.32AATLTYY555 pKa = 10.43GILAEE560 pKa = 4.34ATSKK564 pKa = 11.11ADD566 pKa = 3.65QLILYY571 pKa = 8.59TDD573 pKa = 3.83VSQWDD578 pKa = 3.7ASQHH582 pKa = 4.09NTEE585 pKa = 4.88PYY587 pKa = 9.9RR588 pKa = 11.84SAIINAIKK596 pKa = 10.07EE597 pKa = 3.89ARR599 pKa = 11.84TKK601 pKa = 11.05YY602 pKa = 10.15KK603 pKa = 10.01ISYY606 pKa = 7.25EE607 pKa = 3.97QEE609 pKa = 3.97PKK611 pKa = 10.14VLGMNVLDD619 pKa = 5.15KK620 pKa = 10.67MIEE623 pKa = 3.92IQEE626 pKa = 4.22ALLNSNLLVEE636 pKa = 4.61SQGSKK641 pKa = 10.4RR642 pKa = 11.84PPLKK646 pKa = 9.94IRR648 pKa = 11.84YY649 pKa = 8.39HH650 pKa = 5.84GVASGEE656 pKa = 4.15KK657 pKa = 6.55TTKK660 pKa = 10.0IGNSFANVALITTVFNSLTNSLPSIRR686 pKa = 11.84VNHH689 pKa = 5.66MRR691 pKa = 11.84VDD693 pKa = 3.56GDD695 pKa = 4.26DD696 pKa = 3.95NVVTMYY702 pKa = 9.86TADD705 pKa = 4.92PINVVQEE712 pKa = 4.32AIKK715 pKa = 9.92TKK717 pKa = 10.38YY718 pKa = 10.19KK719 pKa = 11.05DD720 pKa = 2.91MNARR724 pKa = 11.84VKK726 pKa = 10.79ALASYY731 pKa = 9.46TGLEE735 pKa = 3.81MAKK738 pKa = 10.13RR739 pKa = 11.84FIIGGKK745 pKa = 8.6IFEE748 pKa = 4.99RR749 pKa = 11.84GAISIFTAEE758 pKa = 4.3RR759 pKa = 11.84PYY761 pKa = 10.8GTDD764 pKa = 2.76QSVQSTTGSLIYY776 pKa = 10.34SAAVNAYY783 pKa = 9.61RR784 pKa = 11.84GFGDD788 pKa = 4.44GYY790 pKa = 10.86LEE792 pKa = 4.44FMLDD796 pKa = 3.37VLTPPSASIRR806 pKa = 11.84VTGRR810 pKa = 11.84LRR812 pKa = 11.84SLLSPVTLFSTGPISFEE829 pKa = 3.43ITPYY833 pKa = 10.91GLGGRR838 pKa = 11.84MRR840 pKa = 11.84FFQINRR846 pKa = 11.84RR847 pKa = 11.84NMEE850 pKa = 4.02LFKK853 pKa = 10.8ILTSSLSISIEE864 pKa = 3.87PEE866 pKa = 3.66EE867 pKa = 4.09VKK869 pKa = 10.46QYY871 pKa = 11.88SMTPQFKK878 pKa = 10.53ARR880 pKa = 11.84TDD882 pKa = 3.32RR883 pKa = 11.84MIEE886 pKa = 4.06SVRR889 pKa = 11.84IAMKK893 pKa = 10.56SEE895 pKa = 3.74AKK897 pKa = 10.2IITQILVDD905 pKa = 4.0KK906 pKa = 10.27EE907 pKa = 4.11RR908 pKa = 11.84QKK910 pKa = 9.96TLGVPNVATTKK921 pKa = 10.27NRR923 pKa = 11.84QQVEE927 pKa = 4.42KK928 pKa = 10.84ARR930 pKa = 11.84KK931 pKa = 6.81TLSAPKK937 pKa = 9.81EE938 pKa = 4.04VLKK941 pKa = 10.83KK942 pKa = 9.49VSKK945 pKa = 10.46YY946 pKa = 8.33YY947 pKa = 9.95PEE949 pKa = 5.29EE950 pKa = 3.73IFHH953 pKa = 6.33YY954 pKa = 10.24IISNSKK960 pKa = 8.97LHH962 pKa = 6.96KK963 pKa = 9.89SQNDD967 pKa = 3.45EE968 pKa = 3.97KK969 pKa = 11.24VQVYY973 pKa = 8.38MNNSTMIKK981 pKa = 9.62QLQRR985 pKa = 11.84QLGVRR990 pKa = 11.84VAEE993 pKa = 4.9GSHH996 pKa = 5.16LTKK999 pKa = 9.5PTNTLFKK1006 pKa = 10.45LVEE1009 pKa = 3.9KK1010 pKa = 10.28HH1011 pKa = 6.31SPIKK1015 pKa = 10.33ISPSDD1020 pKa = 3.71LVKK1023 pKa = 10.91HH1024 pKa = 5.3SEE1026 pKa = 4.17KK1027 pKa = 10.73YY1028 pKa = 10.41DD1029 pKa = 3.51LSTLQGKK1036 pKa = 9.46KK1037 pKa = 10.15KK1038 pKa = 10.14FLYY1041 pKa = 10.59DD1042 pKa = 3.72LGISGSEE1049 pKa = 3.42LRR1051 pKa = 11.84FYY1053 pKa = 11.3LNSKK1057 pKa = 10.47LLMHH1061 pKa = 7.24DD1062 pKa = 4.58LLLAKK1067 pKa = 10.02YY1068 pKa = 9.39DD1069 pKa = 3.98KK1070 pKa = 10.88LYY1072 pKa = 9.67EE1073 pKa = 4.3APGFGATQLNSLPLDD1088 pKa = 3.64LTAAEE1093 pKa = 4.75KK1094 pKa = 10.88VFSVKK1099 pKa = 10.31VNMPSSYY1106 pKa = 10.65YY1107 pKa = 10.48EE1108 pKa = 4.14LMMLILLYY1116 pKa = 10.42EE1117 pKa = 4.09YY1118 pKa = 11.42VNFVMFTGQTFTCICLPEE1136 pKa = 4.12TTIQSANIAKK1146 pKa = 10.12NVMKK1150 pKa = 10.15MIDD1153 pKa = 4.2NIQLDD1158 pKa = 4.16SVSFQDD1164 pKa = 4.41VIYY1167 pKa = 10.98

Molecular weight:
133.16 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

5693

180

1167

517.5

59.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.499 ± 0.48

1.265 ± 0.371

6.043 ± 0.477

6.253 ± 0.427

4.426 ± 0.315

3.882 ± 0.315

1.862 ± 0.26

7.992 ± 0.32

6.692 ± 0.61

8.221 ± 0.419

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.881 ± 0.251

6.569 ± 0.387

3.952 ± 0.379

3.9 ± 0.307

5.445 ± 0.358

7.096 ± 0.579

6.447 ± 0.477

6.148 ± 0.319

0.755 ± 0.119

3.671 ± 0.428

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski