Porcine rotavirus
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K2SR53|A0A0K2SR53_9REOV NSP2 OS=Porcine rotavirus OX=10913 PE=4 SV=1
MM1 pKa = 7.57 SDD3 pKa = 3.24 VPRR6 pKa = 11.84 FDD8 pKa = 4.66 LRR10 pKa = 11.84 SKK12 pKa = 10.93 RR13 pKa = 11.84 KK14 pKa = 5.54 TQKK17 pKa = 9.38 KK18 pKa = 8.25 QKK20 pKa = 10.39 VDD22 pKa = 3.28 IFDD25 pKa = 4.36 EE26 pKa = 4.48 DD27 pKa = 4.13 DD28 pKa = 3.99 KK29 pKa = 11.62 EE30 pKa = 5.11 SSIQVDD36 pKa = 4.07 GEE38 pKa = 4.3 SDD40 pKa = 3.61 SLASADD46 pKa = 3.44 TSSNHH51 pKa = 6.35 SYY53 pKa = 11.12 EE54 pKa = 5.07 DD55 pKa = 3.49 YY56 pKa = 11.26 SKK58 pKa = 11.08 AYY60 pKa = 9.65 KK61 pKa = 10.4 DD62 pKa = 3.68 LTLEE66 pKa = 4.42 TPVEE70 pKa = 3.98 QPIGDD75 pKa = 4.41 DD76 pKa = 3.71 DD77 pKa = 4.14 QVDD80 pKa = 4.01 SVSTILEE87 pKa = 4.3 SKK89 pKa = 10.42 VDD91 pKa = 3.59 NSWYY95 pKa = 10.22 DD96 pKa = 3.04 KK97 pKa = 10.07 TVEE100 pKa = 4.0 VEE102 pKa = 4.13 EE103 pKa = 4.78 RR104 pKa = 11.84 VQKK107 pKa = 9.76 PKK109 pKa = 11.08 RR110 pKa = 11.84 MTKK113 pKa = 10.02 QKK115 pKa = 10.54 AQQIDD120 pKa = 4.28 EE121 pKa = 4.42 DD122 pKa = 4.24 TSDD125 pKa = 5.23 LKK127 pKa = 11.36 LQLAQLSLRR136 pKa = 11.84 IQKK139 pKa = 8.92 MEE141 pKa = 3.84 SEE143 pKa = 4.5 TRR145 pKa = 11.84 AKK147 pKa = 10.04 TIDD150 pKa = 3.53 SAYY153 pKa = 9.19 HH154 pKa = 5.64 TIITQAEE161 pKa = 4.42 NLTTPQKK168 pKa = 10.61 KK169 pKa = 10.11 SLIAAIIATMRR180 pKa = 3.55
Molecular weight: 20.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.947
IPC2_protein 4.889
IPC_protein 4.8
Toseland 4.647
ProMoST 4.927
Dawson 4.762
Bjellqvist 4.914
Wikipedia 4.66
Rodwell 4.647
Grimsley 4.558
Solomon 4.762
Lehninger 4.724
Nozaki 4.876
DTASelect 5.08
Thurlkill 4.66
EMBOSS 4.673
Sillero 4.927
Patrickios 4.444
IPC_peptide 4.762
IPC2_peptide 4.914
IPC2.peptide.svr19 4.938
Protein with the highest isoelectric point:
>tr|A0A0K2SRD6|A0A0K2SRD6_9REOV NSP3 OS=Porcine rotavirus OX=10913 PE=4 SV=1
MM1 pKa = 8.15 DD2 pKa = 5.07 GANYY6 pKa = 9.77 LAWLARR12 pKa = 11.84 DD13 pKa = 3.39 IVRR16 pKa = 11.84 NLSYY20 pKa = 10.23 TALVYY25 pKa = 10.85 NNPKK29 pKa = 9.62 VAIVTLQDD37 pKa = 3.37 SKK39 pKa = 11.87 EE40 pKa = 3.91 EE41 pKa = 4.22 FISFEE46 pKa = 4.33 KK47 pKa = 9.68 EE48 pKa = 3.49 QKK50 pKa = 9.31 TPSEE54 pKa = 4.74 LIAEE58 pKa = 4.24 IQNIVEE64 pKa = 4.5 SNLSIEE70 pKa = 4.82 DD71 pKa = 4.07 KK72 pKa = 10.92 INNLLTIRR80 pKa = 11.84 YY81 pKa = 8.36 ISVYY85 pKa = 10.67 VDD87 pKa = 3.43 DD88 pKa = 5.87 KK89 pKa = 10.99 SDD91 pKa = 3.17 KK92 pKa = 10.94 RR93 pKa = 11.84 EE94 pKa = 4.08 IVSKK98 pKa = 10.9 LLDD101 pKa = 4.29 KK102 pKa = 10.69 IIKK105 pKa = 8.25 TLEE108 pKa = 4.02 NKK110 pKa = 9.9 VSISEE115 pKa = 4.23 TLKK118 pKa = 10.92 KK119 pKa = 10.81 CIDD122 pKa = 4.62 DD123 pKa = 3.62 IRR125 pKa = 11.84 NEE127 pKa = 4.01 AKK129 pKa = 9.61 SWRR132 pKa = 11.84 IKK134 pKa = 10.64 NSSSFKK140 pKa = 10.09 NYY142 pKa = 9.99 HH143 pKa = 5.38 YY144 pKa = 10.73 NQLVSDD150 pKa = 4.36 FIKK153 pKa = 10.99 YY154 pKa = 10.41 NEE156 pKa = 3.92 FEE158 pKa = 4.06 ILEE161 pKa = 4.75 DD162 pKa = 3.52 EE163 pKa = 5.68 DD164 pKa = 3.83 EE165 pKa = 4.54 YY166 pKa = 11.38 RR167 pKa = 11.84 WKK169 pKa = 10.91 SDD171 pKa = 3.13 TLQGLSPNYY180 pKa = 9.03 NHH182 pKa = 6.95 RR183 pKa = 11.84 THH185 pKa = 6.5 TLISSIIFAVQSRR198 pKa = 11.84 MLRR201 pKa = 11.84 YY202 pKa = 9.56 NDD204 pKa = 3.43 EE205 pKa = 4.06 QLEE208 pKa = 4.3 VLLFMFNKK216 pKa = 9.44 IRR218 pKa = 11.84 EE219 pKa = 4.49 CYY221 pKa = 9.17 NDD223 pKa = 4.35 GYY225 pKa = 11.58 LEE227 pKa = 4.31 LLPNRR232 pKa = 11.84 KK233 pKa = 8.54 WSHH236 pKa = 5.4 TIKK239 pKa = 10.85 DD240 pKa = 3.3 LRR242 pKa = 11.84 TNKK245 pKa = 9.68 KK246 pKa = 9.58 IKK248 pKa = 9.6 MYY250 pKa = 9.16 SAKK253 pKa = 9.95 IIHH256 pKa = 6.37 ATCAMISILHH266 pKa = 7.08 ADD268 pKa = 4.29 PIDD271 pKa = 4.2 PYY273 pKa = 11.25 FLAQIVTTFEE283 pKa = 4.59 EE284 pKa = 4.94 IPAHH288 pKa = 6.13 AAKK291 pKa = 10.27 QLSSPMTLYY300 pKa = 10.45 IGVAQIKK307 pKa = 9.84 SNIVISTQRR316 pKa = 11.84 AAEE319 pKa = 4.15 SVASIKK325 pKa = 10.77 PNVSRR330 pKa = 11.84 LDD332 pKa = 3.64 EE333 pKa = 4.35 SQVAEE338 pKa = 4.29 WNEE341 pKa = 3.78 EE342 pKa = 3.39 MDD344 pKa = 3.77 EE345 pKa = 4.19 YY346 pKa = 11.08 HH347 pKa = 6.73 FKK349 pKa = 11.17 SSILVNMMKK358 pKa = 10.23 TNIYY362 pKa = 9.64 DD363 pKa = 3.85 VPVHH367 pKa = 5.62 TFYY370 pKa = 11.2 KK371 pKa = 10.33 IFNCFSATFHH381 pKa = 6.21 TGHH384 pKa = 6.89 RR385 pKa = 11.84 VDD387 pKa = 4.38 NPQDD391 pKa = 3.96 AIEE394 pKa = 4.35 AQVKK398 pKa = 9.4 VEE400 pKa = 3.92 YY401 pKa = 10.13 TSDD404 pKa = 3.3 INRR407 pKa = 11.84 EE408 pKa = 4.04 MYY410 pKa = 9.66 DD411 pKa = 2.82 QYY413 pKa = 11.92 YY414 pKa = 10.45 FLLKK418 pKa = 10.66 RR419 pKa = 11.84 MLTDD423 pKa = 3.07 QFAEE427 pKa = 4.23 YY428 pKa = 10.49 AEE430 pKa = 4.3 EE431 pKa = 4.15 MFHH434 pKa = 7.21 KK435 pKa = 10.7 YY436 pKa = 10.57 NSDD439 pKa = 3.12 VTAEE443 pKa = 4.32 SLASMSNSSNGYY455 pKa = 7.81 SRR457 pKa = 11.84 SVKK460 pKa = 10.62 FIDD463 pKa = 5.01 RR464 pKa = 11.84 EE465 pKa = 4.01 IKK467 pKa = 6.98 TTKK470 pKa = 10.6 KK471 pKa = 9.58 MLHH474 pKa = 6.67 LDD476 pKa = 3.88 DD477 pKa = 6.17 DD478 pKa = 4.61 LSKK481 pKa = 11.35 DD482 pKa = 4.18 LDD484 pKa = 3.72 FTNIGEE490 pKa = 4.39 QISKK494 pKa = 9.63 GIPMGTRR501 pKa = 11.84 NVPARR506 pKa = 11.84 QTRR509 pKa = 11.84 GIFILNWQVAAIQHH523 pKa = 6.29 TIAEE527 pKa = 4.27 FLYY530 pKa = 10.6 KK531 pKa = 9.96 KK532 pKa = 10.11 AKK534 pKa = 9.99 KK535 pKa = 10.15 GGFGATFAEE544 pKa = 4.84 AYY546 pKa = 8.25 VAKK549 pKa = 10.32 AATLTYY555 pKa = 10.43 GILAEE560 pKa = 4.34 ATSKK564 pKa = 11.11 ADD566 pKa = 3.65 QLILYY571 pKa = 8.59 TDD573 pKa = 3.83 VSQWDD578 pKa = 3.7 ASQHH582 pKa = 4.09 NTEE585 pKa = 4.88 PYY587 pKa = 9.9 RR588 pKa = 11.84 SAIINAIKK596 pKa = 10.07 EE597 pKa = 3.89 ARR599 pKa = 11.84 TKK601 pKa = 11.05 YY602 pKa = 10.15 KK603 pKa = 10.01 ISYY606 pKa = 7.25 EE607 pKa = 3.97 QEE609 pKa = 3.97 PKK611 pKa = 10.14 VLGMNVLDD619 pKa = 5.15 KK620 pKa = 10.67 MIEE623 pKa = 3.92 IQEE626 pKa = 4.22 ALLNSNLLVEE636 pKa = 4.61 SQGSKK641 pKa = 10.4 RR642 pKa = 11.84 PPLKK646 pKa = 9.94 IRR648 pKa = 11.84 YY649 pKa = 8.39 HH650 pKa = 5.84 GVASGEE656 pKa = 4.15 KK657 pKa = 6.55 TTKK660 pKa = 10.0 IGNSFANVALITTVFNSLTNSLPSIRR686 pKa = 11.84 VNHH689 pKa = 5.66 MRR691 pKa = 11.84 VDD693 pKa = 3.56 GDD695 pKa = 4.26 DD696 pKa = 3.95 NVVTMYY702 pKa = 9.86 TADD705 pKa = 4.92 PINVVQEE712 pKa = 4.32 AIKK715 pKa = 9.92 TKK717 pKa = 10.38 YY718 pKa = 10.19 KK719 pKa = 11.05 DD720 pKa = 2.91 MNARR724 pKa = 11.84 VKK726 pKa = 10.79 ALASYY731 pKa = 9.46 TGLEE735 pKa = 3.81 MAKK738 pKa = 10.13 RR739 pKa = 11.84 FIIGGKK745 pKa = 8.6 IFEE748 pKa = 4.99 RR749 pKa = 11.84 GAISIFTAEE758 pKa = 4.3 RR759 pKa = 11.84 PYY761 pKa = 10.8 GTDD764 pKa = 2.76 QSVQSTTGSLIYY776 pKa = 10.34 SAAVNAYY783 pKa = 9.61 RR784 pKa = 11.84 GFGDD788 pKa = 4.44 GYY790 pKa = 10.86 LEE792 pKa = 4.44 FMLDD796 pKa = 3.37 VLTPPSASIRR806 pKa = 11.84 VTGRR810 pKa = 11.84 LRR812 pKa = 11.84 SLLSPVTLFSTGPISFEE829 pKa = 3.43 ITPYY833 pKa = 10.91 GLGGRR838 pKa = 11.84 MRR840 pKa = 11.84 FFQINRR846 pKa = 11.84 RR847 pKa = 11.84 NMEE850 pKa = 4.02 LFKK853 pKa = 10.8 ILTSSLSISIEE864 pKa = 3.87 PEE866 pKa = 3.66 EE867 pKa = 4.09 VKK869 pKa = 10.46 QYY871 pKa = 11.88 SMTPQFKK878 pKa = 10.53 ARR880 pKa = 11.84 TDD882 pKa = 3.32 RR883 pKa = 11.84 MIEE886 pKa = 4.06 SVRR889 pKa = 11.84 IAMKK893 pKa = 10.56 SEE895 pKa = 3.74 AKK897 pKa = 10.2 IITQILVDD905 pKa = 4.0 KK906 pKa = 10.27 EE907 pKa = 4.11 RR908 pKa = 11.84 QKK910 pKa = 9.96 TLGVPNVATTKK921 pKa = 10.27 NRR923 pKa = 11.84 QQVEE927 pKa = 4.42 KK928 pKa = 10.84 ARR930 pKa = 11.84 KK931 pKa = 6.81 TLSAPKK937 pKa = 9.81 EE938 pKa = 4.04 VLKK941 pKa = 10.83 KK942 pKa = 9.49 VSKK945 pKa = 10.46 YY946 pKa = 8.33 YY947 pKa = 9.95 PEE949 pKa = 5.29 EE950 pKa = 3.73 IFHH953 pKa = 6.33 YY954 pKa = 10.24 IISNSKK960 pKa = 8.97 LHH962 pKa = 6.96 KK963 pKa = 9.89 SQNDD967 pKa = 3.45 EE968 pKa = 3.97 KK969 pKa = 11.24 VQVYY973 pKa = 8.38 MNNSTMIKK981 pKa = 9.62 QLQRR985 pKa = 11.84 QLGVRR990 pKa = 11.84 VAEE993 pKa = 4.9 GSHH996 pKa = 5.16 LTKK999 pKa = 9.5 PTNTLFKK1006 pKa = 10.45 LVEE1009 pKa = 3.9 KK1010 pKa = 10.28 HH1011 pKa = 6.31 SPIKK1015 pKa = 10.33 ISPSDD1020 pKa = 3.71 LVKK1023 pKa = 10.91 HH1024 pKa = 5.3 SEE1026 pKa = 4.17 KK1027 pKa = 10.73 YY1028 pKa = 10.41 DD1029 pKa = 3.51 LSTLQGKK1036 pKa = 9.46 KK1037 pKa = 10.15 KK1038 pKa = 10.14 FLYY1041 pKa = 10.59 DD1042 pKa = 3.72 LGISGSEE1049 pKa = 3.42 LRR1051 pKa = 11.84 FYY1053 pKa = 11.3 LNSKK1057 pKa = 10.47 LLMHH1061 pKa = 7.24 DD1062 pKa = 4.58 LLLAKK1067 pKa = 10.02 YY1068 pKa = 9.39 DD1069 pKa = 3.98 KK1070 pKa = 10.88 LYY1072 pKa = 9.67 EE1073 pKa = 4.3 APGFGATQLNSLPLDD1088 pKa = 3.64 LTAAEE1093 pKa = 4.75 KK1094 pKa = 10.88 VFSVKK1099 pKa = 10.31 VNMPSSYY1106 pKa = 10.65 YY1107 pKa = 10.48 EE1108 pKa = 4.14 LMMLILLYY1116 pKa = 10.42 EE1117 pKa = 4.09 YY1118 pKa = 11.42 VNFVMFTGQTFTCICLPEE1136 pKa = 4.12 TTIQSANIAKK1146 pKa = 10.12 NVMKK1150 pKa = 10.15 MIDD1153 pKa = 4.2 NIQLDD1158 pKa = 4.16 SVSFQDD1164 pKa = 4.41 VIYY1167 pKa = 10.98
Molecular weight: 133.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.905
IPC2_protein 8.083
IPC_protein 7.936
Toseland 8.478
ProMoST 8.507
Dawson 8.873
Bjellqvist 8.741
Wikipedia 9.033
Rodwell 9.019
Grimsley 8.873
Solomon 8.916
Lehninger 8.873
Nozaki 8.756
DTASelect 8.668
Thurlkill 8.756
EMBOSS 9.004
Sillero 8.916
Patrickios 4.406
IPC_peptide 8.916
IPC2_peptide 7.351
IPC2.peptide.svr19 7.542
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11
0
11
5693
180
1167
517.5
59.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.499 ± 0.48
1.265 ± 0.371
6.043 ± 0.477
6.253 ± 0.427
4.426 ± 0.315
3.882 ± 0.315
1.862 ± 0.26
7.992 ± 0.32
6.692 ± 0.61
8.221 ± 0.419
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.881 ± 0.251
6.569 ± 0.387
3.952 ± 0.379
3.9 ± 0.307
5.445 ± 0.358
7.096 ± 0.579
6.447 ± 0.477
6.148 ± 0.319
0.755 ± 0.119
3.671 ± 0.428
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here