Avon-Heathcote Estuary associated circular virus 18
Average proteome isoelectric point is 7.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C5I9U1|A0A0C5I9U1_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 18 OX=1618241 PE=4 SV=1
MM1 pKa = 7.39 TARR4 pKa = 11.84 NWVFTINNHH13 pKa = 5.94 FSIDD17 pKa = 3.89 LEE19 pKa = 4.24 EE20 pKa = 4.17 EE21 pKa = 3.86 TWNNNLKK28 pKa = 10.67 LLIAVKK34 pKa = 10.16 EE35 pKa = 4.11 VGEE38 pKa = 4.14 QGTEE42 pKa = 3.73 HH43 pKa = 6.09 LQGYY47 pKa = 9.67 LEE49 pKa = 5.39 LIQPRR54 pKa = 11.84 RR55 pKa = 11.84 FSWVKK60 pKa = 9.74 EE61 pKa = 3.94 RR62 pKa = 11.84 LPLAHH67 pKa = 6.77 LEE69 pKa = 4.01 KK70 pKa = 10.64 RR71 pKa = 11.84 RR72 pKa = 11.84 GSKK75 pKa = 9.68 KK76 pKa = 9.84 QAIQYY81 pKa = 8.36 CLKK84 pKa = 9.93 TISLPSQQCSSPSNTSDD101 pKa = 3.53 TSPQDD106 pKa = 3.17 VDD108 pKa = 3.53 STIPLTIIYY117 pKa = 9.0 PPDD120 pKa = 3.45 MNLSDD125 pKa = 5.66 LYY127 pKa = 11.53 ADD129 pKa = 3.75 YY130 pKa = 11.06 CRR132 pKa = 11.84 SKK134 pKa = 8.78 QTKK137 pKa = 9.35 RR138 pKa = 11.84 QILLAEE144 pKa = 4.37 VRR146 pKa = 11.84 QKK148 pKa = 11.09 LSEE151 pKa = 4.23 GVDD154 pKa = 3.46 DD155 pKa = 3.91 LTIANEE161 pKa = 4.11 YY162 pKa = 9.85 FDD164 pKa = 4.34 VWVSNYY170 pKa = 10.11 RR171 pKa = 11.84 AFEE174 pKa = 4.1 RR175 pKa = 11.84 YY176 pKa = 7.39 RR177 pKa = 11.84 TLITPPRR184 pKa = 11.84 NHH186 pKa = 6.44 EE187 pKa = 4.1 VNVIVIQGPTGTGKK201 pKa = 10.7 SKK203 pKa = 9.63 WCLDD207 pKa = 4.09 NYY209 pKa = 10.08 PNAYY213 pKa = 8.18 WKK215 pKa = 10.05 QRR217 pKa = 11.84 SNWWDD222 pKa = 3.77 GYY224 pKa = 10.33 SGHH227 pKa = 6.99 DD228 pKa = 3.28 TVIIDD233 pKa = 4.6 EE234 pKa = 4.88 FYY236 pKa = 10.81 GWLPFDD242 pKa = 4.26 LLLRR246 pKa = 11.84 VCDD249 pKa = 4.56 RR250 pKa = 11.84 YY251 pKa = 10.46 PLLVEE256 pKa = 4.41 SKK258 pKa = 10.15 GGQIQFVAKK267 pKa = 9.65 TIIITSNSVPSSWYY281 pKa = 8.66 RR282 pKa = 11.84 NSYY285 pKa = 9.31 FDD287 pKa = 3.29 SFVRR291 pKa = 11.84 RR292 pKa = 11.84 VSKK295 pKa = 10.47 WMVFPIWGDD304 pKa = 3.29 QLSFTDD310 pKa = 3.83 YY311 pKa = 11.26 SEE313 pKa = 5.89 AIAHH317 pKa = 6.14 FVNNYY322 pKa = 8.86
Molecular weight: 37.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.145
IPC2_protein 6.186
IPC_protein 6.326
Toseland 6.504
ProMoST 6.751
Dawson 6.678
Bjellqvist 6.605
Wikipedia 6.678
Rodwell 6.664
Grimsley 6.605
Solomon 6.678
Lehninger 6.678
Nozaki 6.912
DTASelect 7.073
Thurlkill 7.102
EMBOSS 7.102
Sillero 7.059
Patrickios 3.986
IPC_peptide 6.693
IPC2_peptide 6.825
IPC2.peptide.svr19 6.776
Protein with the highest isoelectric point:
>tr|A0A0C5I9U1|A0A0C5I9U1_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 18 OX=1618241 PE=4 SV=1
MM1 pKa = 8.17 PYY3 pKa = 9.98 RR4 pKa = 11.84 PKK6 pKa = 10.26 PYY8 pKa = 9.88 SRR10 pKa = 11.84 SLVDD14 pKa = 2.84 YY15 pKa = 10.56 RR16 pKa = 11.84 RR17 pKa = 11.84 ASRR20 pKa = 11.84 INYY23 pKa = 9.74 RR24 pKa = 11.84 IGARR28 pKa = 11.84 LANRR32 pKa = 11.84 VGMSFTKK39 pKa = 10.01 TKK41 pKa = 10.5 RR42 pKa = 11.84 KK43 pKa = 9.36 RR44 pKa = 11.84 QTSGRR49 pKa = 11.84 GVTFQRR55 pKa = 11.84 DD56 pKa = 3.0 QEE58 pKa = 4.3 LIYY61 pKa = 10.33 RR62 pKa = 11.84 KK63 pKa = 9.76 RR64 pKa = 11.84 RR65 pKa = 11.84 MPRR68 pKa = 11.84 KK69 pKa = 9.4 RR70 pKa = 11.84 RR71 pKa = 11.84 RR72 pKa = 11.84 RR73 pKa = 11.84 WKK75 pKa = 9.22 NFIRR79 pKa = 11.84 KK80 pKa = 8.66 SVAASEE86 pKa = 4.31 KK87 pKa = 8.8 TLGSRR92 pKa = 11.84 TVVRR96 pKa = 11.84 NQQFSFAVSGVNANSEE112 pKa = 4.53 DD113 pKa = 3.63 IVGQLALYY121 pKa = 9.81 AVQNGGNEE129 pKa = 3.98 YY130 pKa = 10.74 LRR132 pKa = 11.84 DD133 pKa = 3.73 LNRR136 pKa = 11.84 ICSDD140 pKa = 3.33 TDD142 pKa = 3.24 VSTSGKK148 pKa = 10.58 LIFQSGVMDD157 pKa = 3.55 ATITNISSSDD167 pKa = 3.42 DD168 pKa = 3.38 QDD170 pKa = 4.07 RR171 pKa = 11.84 KK172 pKa = 10.75 DD173 pKa = 3.67 VPIEE177 pKa = 3.57 LDD179 pKa = 3.03 IYY181 pKa = 10.37 EE182 pKa = 4.14 VSARR186 pKa = 11.84 RR187 pKa = 11.84 NFEE190 pKa = 3.9 KK191 pKa = 10.58 GTGDD195 pKa = 5.35 KK196 pKa = 11.33 GLLDD200 pKa = 4.14 CFAEE204 pKa = 4.84 GFLDD208 pKa = 4.3 QGLLSTTGTDD218 pKa = 3.31 TPLSLTTRR226 pKa = 11.84 GSTPWDD232 pKa = 3.73 CPQALSMYY240 pKa = 10.21 RR241 pKa = 11.84 LKK243 pKa = 10.22 IWKK246 pKa = 6.87 KK247 pKa = 7.45 TKK249 pKa = 9.7 YY250 pKa = 9.4 RR251 pKa = 11.84 LSGGQSLSYY260 pKa = 10.66 QMRR263 pKa = 11.84 DD264 pKa = 3.4 PKK266 pKa = 10.48 RR267 pKa = 11.84 HH268 pKa = 4.78 VMEE271 pKa = 4.74 KK272 pKa = 10.52 GYY274 pKa = 10.81 VGEE277 pKa = 4.23 LTGSNLPGVTRR288 pKa = 11.84 WLILIAKK295 pKa = 7.95 PIVGFNVNFNGIAEE309 pKa = 4.1 IRR311 pKa = 11.84 VGVTRR316 pKa = 11.84 KK317 pKa = 9.85 YY318 pKa = 10.59 LYY320 pKa = 10.51 KK321 pKa = 10.65 LNEE324 pKa = 3.96 RR325 pKa = 11.84 NEE327 pKa = 4.35 DD328 pKa = 3.17 KK329 pKa = 10.99 DD330 pKa = 3.95 ALVTT334 pKa = 3.91
Molecular weight: 38.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.283
IPC2_protein 9.589
IPC_protein 10.014
Toseland 10.365
ProMoST 10.028
Dawson 10.511
Bjellqvist 10.189
Wikipedia 10.701
Rodwell 10.847
Grimsley 10.584
Solomon 10.555
Lehninger 10.526
Nozaki 10.335
DTASelect 10.189
Thurlkill 10.379
EMBOSS 10.76
Sillero 10.438
Patrickios 10.496
IPC_peptide 10.57
IPC2_peptide 8.96
IPC2.peptide.svr19 8.694
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
656
322
334
328.0
37.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.268 ± 0.376
1.22 ± 0.231
5.945 ± 0.031
4.573 ± 0.491
3.659 ± 0.263
6.25 ± 1.321
1.067 ± 0.553
5.945 ± 0.616
5.945 ± 0.678
8.537 ± 0.11
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.524 ± 0.412
5.488 ± 0.287
4.116 ± 0.592
4.421 ± 0.381
8.537 ± 1.831
8.384 ± 0.216
6.402 ± 0.348
6.555 ± 0.023
2.439 ± 0.894
4.726 ± 0.385
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here