Amino acid dipepetide frequency for Mycobacterium phage SuperGrey

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.736AlaAla: 14.736 ± 1.824
0.937AlaCys: 0.937 ± 0.205
7.602AlaAsp: 7.602 ± 0.864
7.654AlaGlu: 7.654 ± 0.852
2.916AlaPhe: 2.916 ± 0.414
9.945AlaGly: 9.945 ± 1.324
2.812AlaHis: 2.812 ± 0.43
4.27AlaIle: 4.27 ± 0.517
4.061AlaLys: 4.061 ± 0.525
8.383AlaLeu: 8.383 ± 0.803
2.239AlaMet: 2.239 ± 0.403
2.708AlaAsn: 2.708 ± 0.436
4.79AlaPro: 4.79 ± 0.775
2.864AlaGln: 2.864 ± 0.473
7.706AlaArg: 7.706 ± 0.759
5.207AlaSer: 5.207 ± 0.644
5.884AlaThr: 5.884 ± 0.471
6.717AlaVal: 6.717 ± 0.646
2.916AlaTrp: 2.916 ± 0.49
2.083AlaTyr: 2.083 ± 0.334
0.0AlaXaa: 0.0 ± 0.0
Cys
0.989CysAla: 0.989 ± 0.286
0.156CysCys: 0.156 ± 0.085
1.51CysAsp: 1.51 ± 0.348
0.573CysGlu: 0.573 ± 0.159
0.364CysPhe: 0.364 ± 0.138
1.354CysGly: 1.354 ± 0.312
0.364CysHis: 0.364 ± 0.139
0.0CysIle: 0.0 ± 0.0
0.521CysLys: 0.521 ± 0.149
0.521CysLeu: 0.521 ± 0.184
0.26CysMet: 0.26 ± 0.104
0.417CysAsn: 0.417 ± 0.145
0.937CysPro: 0.937 ± 0.236
0.312CysGln: 0.312 ± 0.166
1.093CysArg: 1.093 ± 0.279
0.417CysSer: 0.417 ± 0.162
0.677CysThr: 0.677 ± 0.163
0.885CysVal: 0.885 ± 0.211
0.208CysTrp: 0.208 ± 0.094
0.26CysTyr: 0.26 ± 0.105
0.0CysXaa: 0.0 ± 0.0
Asp
6.821AspAla: 6.821 ± 0.521
0.937AspCys: 0.937 ± 0.248
4.634AspAsp: 4.634 ± 0.5
3.437AspGlu: 3.437 ± 0.462
1.666AspPhe: 1.666 ± 0.257
6.821AspGly: 6.821 ± 0.671
1.51AspHis: 1.51 ± 0.242
2.447AspIle: 2.447 ± 0.333
1.562AspLys: 1.562 ± 0.289
6.144AspLeu: 6.144 ± 0.51
1.041AspMet: 1.041 ± 0.242
1.25AspAsn: 1.25 ± 0.386
4.53AspPro: 4.53 ± 0.527
2.291AspGln: 2.291 ± 0.323
5.155AspArg: 5.155 ± 0.624
3.385AspSer: 3.385 ± 0.46
3.957AspThr: 3.957 ± 0.417
4.114AspVal: 4.114 ± 0.503
1.666AspTrp: 1.666 ± 0.292
1.979AspTyr: 1.979 ± 0.276
0.0AspXaa: 0.0 ± 0.0
Glu
6.925GluAla: 6.925 ± 0.699
0.729GluCys: 0.729 ± 0.207
3.332GluAsp: 3.332 ± 0.403
3.02GluGlu: 3.02 ± 0.588
2.239GluPhe: 2.239 ± 0.392
3.228GluGly: 3.228 ± 0.417
1.614GluHis: 1.614 ± 0.367
2.447GluIle: 2.447 ± 0.357
1.822GluLys: 1.822 ± 0.264
5.728GluLeu: 5.728 ± 0.637
1.354GluMet: 1.354 ± 0.274
1.822GluAsn: 1.822 ± 0.255
2.708GluPro: 2.708 ± 0.41
3.176GluGln: 3.176 ± 0.409
5.051GluArg: 5.051 ± 0.597
3.228GluSer: 3.228 ± 0.528
3.697GluThr: 3.697 ± 0.543
4.218GluVal: 4.218 ± 0.505
1.51GluTrp: 1.51 ± 0.264
1.822GluTyr: 1.822 ± 0.311
0.0GluXaa: 0.0 ± 0.0
Phe
3.228PheAla: 3.228 ± 0.434
0.312PheCys: 0.312 ± 0.134
2.708PheAsp: 2.708 ± 0.517
1.354PheGlu: 1.354 ± 0.308
0.833PhePhe: 0.833 ± 0.229
2.968PheGly: 2.968 ± 0.617
0.677PheHis: 0.677 ± 0.247
1.25PheIle: 1.25 ± 0.317
0.885PheLys: 0.885 ± 0.261
1.979PheLeu: 1.979 ± 0.27
0.677PheMet: 0.677 ± 0.248
0.937PheAsn: 0.937 ± 0.284
1.614PhePro: 1.614 ± 0.287
1.146PheGln: 1.146 ± 0.31
1.458PheArg: 1.458 ± 0.295
1.562PheSer: 1.562 ± 0.294
2.656PheThr: 2.656 ± 0.368
2.291PheVal: 2.291 ± 0.282
0.781PheTrp: 0.781 ± 0.172
0.729PheTyr: 0.729 ± 0.251
0.0PheXaa: 0.0 ± 0.0
Gly
8.539GlyAla: 8.539 ± 1.294
1.25GlyCys: 1.25 ± 0.307
5.415GlyAsp: 5.415 ± 0.568
4.426GlyGlu: 4.426 ± 0.508
2.603GlyPhe: 2.603 ± 0.436
10.778GlyGly: 10.778 ± 2.964
1.718GlyHis: 1.718 ± 0.274
4.009GlyIle: 4.009 ± 0.507
2.656GlyLys: 2.656 ± 0.335
6.196GlyLeu: 6.196 ± 0.543
2.291GlyMet: 2.291 ± 0.374
2.812GlyAsn: 2.812 ± 0.438
4.426GlyPro: 4.426 ± 0.605
2.916GlyGln: 2.916 ± 0.556
5.155GlyArg: 5.155 ± 0.606
5.936GlySer: 5.936 ± 0.943
6.248GlyThr: 6.248 ± 0.795
6.248GlyVal: 6.248 ± 0.666
2.291GlyTrp: 2.291 ± 0.357
2.187GlyTyr: 2.187 ± 0.354
0.0GlyXaa: 0.0 ± 0.0
His
1.927HisAla: 1.927 ± 0.338
0.208HisCys: 0.208 ± 0.144
1.354HisAsp: 1.354 ± 0.265
1.614HisGlu: 1.614 ± 0.314
0.364HisPhe: 0.364 ± 0.113
2.031HisGly: 2.031 ± 0.317
0.937HisHis: 0.937 ± 0.235
1.718HisIle: 1.718 ± 0.35
0.937HisLys: 0.937 ± 0.206
1.406HisLeu: 1.406 ± 0.279
0.573HisMet: 0.573 ± 0.177
0.833HisAsn: 0.833 ± 0.215
1.354HisPro: 1.354 ± 0.223
1.041HisGln: 1.041 ± 0.225
1.979HisArg: 1.979 ± 0.321
0.989HisSer: 0.989 ± 0.227
1.302HisThr: 1.302 ± 0.298
1.562HisVal: 1.562 ± 0.343
0.573HisTrp: 0.573 ± 0.182
0.729HisTyr: 0.729 ± 0.18
0.0HisXaa: 0.0 ± 0.0
Ile
4.842IleAla: 4.842 ± 0.451
0.364IleCys: 0.364 ± 0.153
4.009IleAsp: 4.009 ± 0.548
3.541IleGlu: 3.541 ± 0.38
0.989IlePhe: 0.989 ± 0.278
3.645IleGly: 3.645 ± 0.445
1.458IleHis: 1.458 ± 0.304
1.302IleIle: 1.302 ± 0.249
1.25IleLys: 1.25 ± 0.242
1.875IleLeu: 1.875 ± 0.354
0.469IleMet: 0.469 ± 0.153
2.083IleAsn: 2.083 ± 0.349
2.916IlePro: 2.916 ± 0.362
1.614IleGln: 1.614 ± 0.241
2.395IleArg: 2.395 ± 0.387
2.291IleSer: 2.291 ± 0.407
3.593IleThr: 3.593 ± 0.418
3.228IleVal: 3.228 ± 0.399
0.833IleTrp: 0.833 ± 0.177
0.833IleTyr: 0.833 ± 0.185
0.0IleXaa: 0.0 ± 0.0
Lys
4.061LysAla: 4.061 ± 0.5
0.521LysCys: 0.521 ± 0.177
1.77LysAsp: 1.77 ± 0.31
1.562LysGlu: 1.562 ± 0.268
1.198LysPhe: 1.198 ± 0.215
2.343LysGly: 2.343 ± 0.316
1.146LysHis: 1.146 ± 0.269
0.989LysIle: 0.989 ± 0.26
1.093LysLys: 1.093 ± 0.27
2.76LysLeu: 2.76 ± 0.366
0.677LysMet: 0.677 ± 0.161
0.885LysAsn: 0.885 ± 0.19
2.135LysPro: 2.135 ± 0.278
1.77LysGln: 1.77 ± 0.3
2.395LysArg: 2.395 ± 0.335
1.718LysSer: 1.718 ± 0.273
2.031LysThr: 2.031 ± 0.324
2.499LysVal: 2.499 ± 0.375
0.833LysTrp: 0.833 ± 0.265
0.885LysTyr: 0.885 ± 0.236
0.0LysXaa: 0.0 ± 0.0
Leu
8.175LeuAla: 8.175 ± 0.815
0.729LeuCys: 0.729 ± 0.261
4.322LeuAsp: 4.322 ± 0.584
4.478LeuGlu: 4.478 ± 0.507
2.447LeuPhe: 2.447 ± 0.365
5.571LeuGly: 5.571 ± 0.693
0.937LeuHis: 0.937 ± 0.211
3.593LeuIle: 3.593 ± 0.447
1.875LeuLys: 1.875 ± 0.344
4.634LeuLeu: 4.634 ± 0.546
1.822LeuMet: 1.822 ± 0.386
2.812LeuAsn: 2.812 ± 0.313
4.895LeuPro: 4.895 ± 0.676
2.864LeuGln: 2.864 ± 0.388
5.415LeuArg: 5.415 ± 0.63
5.415LeuSer: 5.415 ± 0.506
5.728LeuThr: 5.728 ± 0.612
4.582LeuVal: 4.582 ± 0.499
1.562LeuTrp: 1.562 ± 0.316
2.083LeuTyr: 2.083 ± 0.371
0.0LeuXaa: 0.0 ± 0.0
Met
2.239MetAla: 2.239 ± 0.38
0.312MetCys: 0.312 ± 0.125
1.146MetAsp: 1.146 ± 0.255
1.354MetGlu: 1.354 ± 0.239
0.729MetPhe: 0.729 ± 0.21
1.51MetGly: 1.51 ± 0.308
0.104MetHis: 0.104 ± 0.086
1.146MetIle: 1.146 ± 0.24
0.781MetLys: 0.781 ± 0.221
1.718MetLeu: 1.718 ± 0.26
0.677MetMet: 0.677 ± 0.223
0.937MetAsn: 0.937 ± 0.211
1.25MetPro: 1.25 ± 0.258
0.26MetGln: 0.26 ± 0.098
1.51MetArg: 1.51 ± 0.318
2.864MetSer: 2.864 ± 0.417
2.135MetThr: 2.135 ± 0.284
1.458MetVal: 1.458 ± 0.325
0.208MetTrp: 0.208 ± 0.108
0.364MetTyr: 0.364 ± 0.123
0.0MetXaa: 0.0 ± 0.0
Asn
3.228AsnAla: 3.228 ± 0.45
0.26AsnCys: 0.26 ± 0.146
1.822AsnAsp: 1.822 ± 0.311
1.51AsnGlu: 1.51 ± 0.247
0.833AsnPhe: 0.833 ± 0.266
4.009AsnGly: 4.009 ± 0.895
0.937AsnHis: 0.937 ± 0.184
1.562AsnIle: 1.562 ± 0.46
0.989AsnLys: 0.989 ± 0.195
2.239AsnLeu: 2.239 ± 0.346
0.625AsnMet: 0.625 ± 0.15
1.77AsnAsn: 1.77 ± 0.335
2.395AsnPro: 2.395 ± 0.304
0.989AsnGln: 0.989 ± 0.298
2.395AsnArg: 2.395 ± 0.436
1.406AsnSer: 1.406 ± 0.279
2.083AsnThr: 2.083 ± 0.305
1.927AsnVal: 1.927 ± 0.346
0.625AsnTrp: 0.625 ± 0.18
0.625AsnTyr: 0.625 ± 0.149
0.0AsnXaa: 0.0 ± 0.0
Pro
5.311ProAla: 5.311 ± 0.59
0.885ProCys: 0.885 ± 0.218
3.697ProAsp: 3.697 ± 0.435
4.166ProGlu: 4.166 ± 0.399
1.875ProPhe: 1.875 ± 0.327
6.561ProGly: 6.561 ± 0.639
1.822ProHis: 1.822 ± 0.337
2.187ProIle: 2.187 ± 0.345
2.187ProLys: 2.187 ± 0.339
4.166ProLeu: 4.166 ± 0.521
1.77ProMet: 1.77 ± 0.371
1.875ProAsn: 1.875 ± 0.3
3.749ProPro: 3.749 ± 0.533
2.083ProGln: 2.083 ± 0.292
3.176ProArg: 3.176 ± 0.479
3.124ProSer: 3.124 ± 0.434
3.541ProThr: 3.541 ± 0.556
4.27ProVal: 4.27 ± 0.585
1.198ProTrp: 1.198 ± 0.244
1.51ProTyr: 1.51 ± 0.284
0.0ProXaa: 0.0 ± 0.0
Gln
4.166GlnAla: 4.166 ± 0.448
0.312GlnCys: 0.312 ± 0.132
1.51GlnAsp: 1.51 ± 0.248
1.614GlnGlu: 1.614 ± 0.364
1.041GlnPhe: 1.041 ± 0.237
2.239GlnGly: 2.239 ± 0.447
0.573GlnHis: 0.573 ± 0.169
1.77GlnIle: 1.77 ± 0.314
1.562GlnLys: 1.562 ± 0.221
3.385GlnLeu: 3.385 ± 0.36
0.677GlnMet: 0.677 ± 0.177
0.937GlnAsn: 0.937 ± 0.281
2.499GlnPro: 2.499 ± 0.365
1.458GlnGln: 1.458 ± 0.26
2.603GlnArg: 2.603 ± 0.395
2.343GlnSer: 2.343 ± 0.3
1.822GlnThr: 1.822 ± 0.343
2.708GlnVal: 2.708 ± 0.36
0.625GlnTrp: 0.625 ± 0.186
0.833GlnTyr: 0.833 ± 0.306
0.0GlnXaa: 0.0 ± 0.0
Arg
6.3ArgAla: 6.3 ± 0.532
1.093ArgCys: 1.093 ± 0.291
4.582ArgAsp: 4.582 ± 0.524
4.686ArgGlu: 4.686 ± 0.641
2.499ArgPhe: 2.499 ± 0.438
3.697ArgGly: 3.697 ± 0.425
1.51ArgHis: 1.51 ± 0.37
3.905ArgIle: 3.905 ± 0.511
2.916ArgLys: 2.916 ± 0.402
5.467ArgLeu: 5.467 ± 0.65
1.927ArgMet: 1.927 ± 0.344
2.083ArgAsn: 2.083 ± 0.318
3.385ArgPro: 3.385 ± 0.458
2.187ArgGln: 2.187 ± 0.411
5.728ArgArg: 5.728 ± 0.813
4.582ArgSer: 4.582 ± 0.596
3.176ArgThr: 3.176 ± 0.481
5.676ArgVal: 5.676 ± 0.759
2.083ArgTrp: 2.083 ± 0.329
1.875ArgTyr: 1.875 ± 0.287
0.0ArgXaa: 0.0 ± 0.0
Ser
6.457SerAla: 6.457 ± 1.331
0.364SerCys: 0.364 ± 0.151
4.009SerAsp: 4.009 ± 0.507
3.28SerGlu: 3.28 ± 0.462
1.875SerPhe: 1.875 ± 0.385
6.457SerGly: 6.457 ± 0.731
1.198SerHis: 1.198 ± 0.205
2.656SerIle: 2.656 ± 0.375
2.395SerLys: 2.395 ± 0.369
3.489SerLeu: 3.489 ± 0.491
1.51SerMet: 1.51 ± 0.292
2.187SerAsn: 2.187 ± 0.351
3.749SerPro: 3.749 ± 0.397
1.406SerGln: 1.406 ± 0.276
3.28SerArg: 3.28 ± 0.383
4.114SerSer: 4.114 ± 0.561
3.332SerThr: 3.332 ± 0.495
4.947SerVal: 4.947 ± 0.598
1.666SerTrp: 1.666 ± 0.246
1.146SerTyr: 1.146 ± 0.2
0.0SerXaa: 0.0 ± 0.0
Thr
7.081ThrAla: 7.081 ± 0.617
0.625ThrCys: 0.625 ± 0.2
4.009ThrAsp: 4.009 ± 0.569
3.905ThrGlu: 3.905 ± 0.397
1.77ThrPhe: 1.77 ± 0.366
6.405ThrGly: 6.405 ± 0.735
1.666ThrHis: 1.666 ± 0.339
3.072ThrIle: 3.072 ± 0.425
1.875ThrLys: 1.875 ± 0.281
4.686ThrLeu: 4.686 ± 0.591
1.302ThrMet: 1.302 ± 0.241
2.499ThrAsn: 2.499 ± 0.418
5.259ThrPro: 5.259 ± 0.728
1.666ThrGln: 1.666 ± 0.299
3.593ThrArg: 3.593 ± 0.335
3.645ThrSer: 3.645 ± 0.417
4.374ThrThr: 4.374 ± 0.629
5.311ThrVal: 5.311 ± 0.546
1.093ThrTrp: 1.093 ± 0.258
1.875ThrTyr: 1.875 ± 0.427
0.0ThrXaa: 0.0 ± 0.0
Val
6.925ValAla: 6.925 ± 0.6
1.302ValCys: 1.302 ± 0.259
4.947ValAsp: 4.947 ± 0.487
4.947ValGlu: 4.947 ± 0.661
2.395ValPhe: 2.395 ± 0.34
5.259ValGly: 5.259 ± 0.607
1.302ValHis: 1.302 ± 0.251
3.02ValIle: 3.02 ± 0.442
2.083ValLys: 2.083 ± 0.273
5.207ValLeu: 5.207 ± 0.592
1.666ValMet: 1.666 ± 0.277
1.927ValAsn: 1.927 ± 0.303
4.114ValPro: 4.114 ± 0.43
2.864ValGln: 2.864 ± 0.358
5.311ValArg: 5.311 ± 0.598
4.634ValSer: 4.634 ± 0.493
6.144ValThr: 6.144 ± 0.549
5.936ValVal: 5.936 ± 0.655
1.77ValTrp: 1.77 ± 0.296
1.406ValTyr: 1.406 ± 0.295
0.0ValXaa: 0.0 ± 0.0
Trp
2.239TrpAla: 2.239 ± 0.341
0.208TrpCys: 0.208 ± 0.09
1.614TrpAsp: 1.614 ± 0.307
0.937TrpGlu: 0.937 ± 0.286
0.781TrpPhe: 0.781 ± 0.228
1.198TrpGly: 1.198 ± 0.23
0.573TrpHis: 0.573 ± 0.182
1.041TrpIle: 1.041 ± 0.202
1.041TrpLys: 1.041 ± 0.212
1.822TrpLeu: 1.822 ± 0.329
0.781TrpMet: 0.781 ± 0.228
0.625TrpAsn: 0.625 ± 0.223
0.989TrpPro: 0.989 ± 0.237
1.093TrpGln: 1.093 ± 0.256
1.875TrpArg: 1.875 ± 0.378
1.718TrpSer: 1.718 ± 0.385
1.614TrpThr: 1.614 ± 0.283
2.395TrpVal: 2.395 ± 0.437
0.989TrpTrp: 0.989 ± 0.203
0.208TrpTyr: 0.208 ± 0.096
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.291TyrAla: 2.291 ± 0.296
0.312TyrCys: 0.312 ± 0.117
1.354TyrAsp: 1.354 ± 0.329
1.614TyrGlu: 1.614 ± 0.289
0.625TyrPhe: 0.625 ± 0.188
2.083TyrGly: 2.083 ± 0.371
0.573TyrHis: 0.573 ± 0.198
1.146TyrIle: 1.146 ± 0.215
0.781TyrLys: 0.781 ± 0.197
2.135TyrLeu: 2.135 ± 0.341
0.312TyrMet: 0.312 ± 0.127
0.833TyrAsn: 0.833 ± 0.244
1.458TyrPro: 1.458 ± 0.265
0.677TyrGln: 0.677 ± 0.182
2.083TyrArg: 2.083 ± 0.393
0.937TyrSer: 0.937 ± 0.225
1.718TyrThr: 1.718 ± 0.352
2.187TyrVal: 2.187 ± 0.268
0.312TyrTrp: 0.312 ± 0.117
0.729TyrTyr: 0.729 ± 0.208
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 103 proteins (19206 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski